생리장해 저항성 대목 배연 “3호” 선발

조광식(Kwang-Sik Cho),강삼석(Sam-Seok Kang),손동수(Dong-Su Son),송장훈(Jang-Hoo Song),김윤경(Yoon-Kyeong Kim),정상복(Sang-Bouk Jeong),신일섭(Il-Sheob Shin),김명수(Myung-Su Kim) 한국생물환경조절학회 2007 한국생물환경조절학회 학술발표논문집 Vol.16 No.2

실험실에서 제조된 인체 표피에서 표피 단백항원 및 기저막 구성 성분의 발현

조광현 ( Kwang Hyun Cho ),이동윤 ( Dong Youn Lee ),은희철 ( Hee Chul Eun ),박경찬 ( Kyoung Chan Park ),윤재일 ( Jai Il Youn ) 대한피부과학회 1996 대한피부과학회지 Vol.34 No.2

Background: A number of in vitro skin models have been developed for the purpose of the screening of cosmetics, pharmaceuticals, and environmental chemicals. To mimic the skin in vivo, a model should resemble morphologically and biochemically the parent, tissue. Objective : The purpos of this study is to study the differentiation and organization of the artificial epidermis in comparsion with epidermis in vivo based on the expression of epidermal protein antigens and basement membrane components. Methods : Human keratinocytes were cultured on deepidermidized dermis (RE-DED) or on fibroblast-populated collag-,n matrix (LSE). After 10 days culture, the sections of RE-DED and LSE were stained with hematoxylin and eosin. An immunohistochemical study was also performed with the sections of RE-DED and LSE using antibodies recognizing proliferating cell nuclear antigens (PCNA), epidermal growth factor receptor (EGFR), keratin 1, involucrin, filaggrin, loricrin, keratin 13, type IV collagen, and laminin. Results : In both culture systems(RE-DED and LSE) a multilayered epidermis with a horny layer was observed. In the human epidermis reconstructed by both culture systems, differentiation markers appeared but with a topography slightly different from that of epidermis in vivo, and components of the basement membrane was also expressed. Conclusion : Our findings suggest the epidermis obtained in both culture systems(RE-DED and LSE) resembled in vivo epidermis morphologically and biochemically, although it was not the same. (Kor J Dermatol 1996;34(2): 264-272)

유전 및 육종 : 홀스타인의 국제유전평가를 위한 모형개발에 관한 연구

조광현 ( Kwang Hyun Cho ),박병호 ( Byoung Ho Park ),최재관 ( Jac Kwan Choi ),최대과 ( Dae Kwa Choi ),최태정 ( Tae Jeong Choi ),최연호 ( Yun Ho Choy ),이승수 ( Seung Su Lee ),조총일 ( Chung Il Cho ) 한국동물자원과학회(구 한국축산학회) 2013 한국축산학회지 Vol.55 No.1

본 연구는 국내 국가단위 평가결과의 문제점을 해결하면서 유전능력평가시스템을 고도화하고 우리나라가 국제유전평가에 참여하기 위하여 국제평가기구에서 요구하는 검증작업을 통과하기 위하여 수행하였다. 본 연구에 이용된 자료는 농협중앙회 젖소개량부에서 수집한 분만일이 2001년부터 2009년까지의 검정성적으로 총 1,416,589개이며 산차는 5산으로 제한하였으며, 누적착유일은 75~305일로 제한하였고, 전체 혈통자료는 2,279,741개이며 부모를 갖는 개체는 535,409개이고 아비소는 2,467두로 구성되어 있는 기록들을 이용하였다. 유량, 유지방, 유단백에 대한 육종가는 신규로 개발한 다형질모형(Multiple traits model)을 이용하여 육종가를 구하였으며 기초작업은 SAS version 9.2와 R프로그램을 이용하였으며 유전모수를 추정하기 위하여 VCE 6.0을 이용하였다. 전반적으로 유전적 추세는 꾸준히 지속되어 오고 있으며 산차별 차이가 두드러지게 나타나지는 않았다. 유지방을 제외하고 유량과 유단백 형질의 추세가 잘 추정되었음을 알 수 있다. 유전평가분석결과 상위 1000두의 랭킹안에 최근의 생년을 갖는 딸소가 기존에 사용한 모수로 분석한 결과보다 딸소가 증가했음을 알 수 있다. 국제유전평가모형을 통해 새로 평가한 결과 씨수소상위 100두를 기존 평가모형에 의한 평가결과와 비교했을 때, 2006년생은 23두에서 28두로 2007년생은 12두에서 20두로 2008년생은 2두에서 8두로 증가하였다. 이는 최근의 딸소나 씨수소의 유전능력이 우수함에도 불구하고 상위에 랭크되지 못했던 결과가 새 모형의 적용으로 보완하여 새로운 평가분석에 적용할 수 있을 것으로 판단된다. 따라서 기존의 단형질 모형 대신 다형질 모형을 이용한 분석방법으로 평가를 실시하고 국제유전평가에도 다형질 모형을 이용한 육종가를 제시함으로써 다형질 선발의 정확도가 향상될 수 있을 것이다. This study was aimed to solve the problems of current national genetic evaluation systems in Korea and its development to pass the verification processes as required by International Bull Evaluation Service(Interbull). This will enable Korea to participate in international genetic evaluation program. A total of 1,416,589 test-day milk records with calving dates used in this study were collected by National Agricultural Cooperative Federation from 2001 to 2009. Parity was limited up to fifth calving and milk production records were adjusted to cumulative 305 day lactation. The pedigree consisted of 2,279,741 animals where 2,467 bulls had 535,409 parents. A newly developed multiple trait model was used in calculation of breeding values for milk yield, milk fat, and protein yield. Data were edited with SAS(version 9.2) and R programs, and genetic parameters were estimated using VCE 6.0. Results showed a continuous increase in genetic potentials, in general, and no remarkable differences were found between performances by parity. Except fat yield, potentials in milk yield and protein yield were well calculated. We found an increased number of daughters per each top ranked 1,000 bulls in recent years of calf births compared to the cases of previous evaluations. Of the bulls ranked top 100 by our new models(multiple-trait models) we found that increased numbers of bulls were included. Of twenty eight bulls born in 2006, twenty bulls born in 2007 and eight bulls born in 2008 that were listed by new models, only 23, 12, and 2 bulls born in respective years were represented on top 100 by old single-trait models. Re-ranking of the daughters or sires by multiple-trait models suggest that this new multiple trait approach should be used for dairy cattle genetic evaluation and seed-stock selection in the future to increase the accuracy of multiple trait selection. Breeding values for these traits should also be calculated by new method for international genetic evaluation.

홀스타인 젖소의 케톤증과 관련된 원유속 아세톤과 β-히드록시부틸산 함량에 대한 (공)유전력

조광현,조충일,이준호,박경도,Cho, Kwang-Hyun,Cho, Chung-Il,Lee, Joon-Ho,Park, Kyung-Do 한국데이터정보과학회 2015 한국데이터정보과학회지 Vol.26 No.4

This experiment was conducted to estimate the heritability and coheritablity of daily milk yield, acetone and ${\beta}$-hydroxybutyrate (BHBA) concentrations in raw milk. The average concentrations of acetone and BHBA were $135.54{\pm}96.29{\mu}mol$ and $61.08{\pm}66.76{\mu}mol$, respectively, and the differences between high group and low group cows were highly significant (p <0.01). The estimates of heritability of daily milk yield, acetone and BHBA concentrations were in the range of 0.18~0.21, 0.11~0.13 and 0.01~0.02, respectively. The estimate of heritability of $Log_e$acetone did not change much, while that of $Log_eBHBA$ increased to 0.03~0.04. The estimates of phenotypic and genetic correlation coefficients between acetone and BHBA were 0.44 and 0.48, respectively. In low milk yield group, the coheritability estimates of BHBA and $Log_eBHBA$ when selection was for daily milk yield were 0.26 and 0.32, respectively. These were higher than the coheritability estimate of acetone when selection was for daily milk yield. The same trend was noted in the coherihability estimates from the whole records using both high and low milk yield groups together. BHBA concentration seemed to be more effectively responding than acetone concentration when selection was for daily milk yield. 본 연구는 젖소 35,645두의 검정기록, 총 294,834를 이용하여 1회 착유량과 아세톤 및 ${\beta}$-히드록시부틸산 (BHBA) 함량의 (공)유전력을 추정하기 위하여 수행되었다. 아세톤과 BHBA의 평균 함량은 원유 1리터당 각각 $135.54{\pm}96.29{\mu}mol$과 $61.08{\pm}66.76{\mu}mol$였으며, 상위군과 하위군간 아세톤과 BHBA 함량의 차이는 각각 $6.12{\mu}mol/L$과 $4.35{\mu}mol/L$로서 모두 유의적인 차이(p<0.01)를 나타내었다. 1회 착유량의 유전력은 0.18~0.21, 아세톤 함량은 0.11~0.13과 BHBA 함량은 0.01~0.02의 범위에서 추정되었다. 아세톤과 BHBA의 표현형상관과 유전상관은 각각 0.44와 0.48로 추정되었으며, BHBA 함량이 $500{\mu}mol/L$ 이상부터는 아세톤과의 상관관계가 현저히 낮아지는 현상을 나타내었다. 하위군에서 1회 착유량에 대한 아세톤과 $Log_e$아세톤의 공유전력은 각각 0.02와 0.16으로 추정된 반면, BHBA와 $Log_eBHBA$의 공유전력은 각각 0.26과 0.32로 아세톤의 추정치보다 높게 나타났으며, 이러한 결과는 전체자료로 추정된 결과와도 일치하였다. 따라서 아세톤 함량보다는 1회 착유량과 공유전력이 높은 BHBA 함량이 1회 착유량에 대한 선발을 통하여 간접선발 반응을 유도하는데 더 효과적이며, 지시형질로서 바람직하였다.

유전 및 육종 : 젖소 국제유전능력 평가를 위한 종모우별 다형질 Effective Daughter Contribution 추정

조광현 ( Kwang Hyun Cho ),최태정 ( Tae Jeong Choi ),조충일 ( Chung Il Cho ),박경도 ( Kyung Do Park ),도경탁 ( Kyoung Tag Do ),오재돈 ( Jae Don Oh ),이학교 ( Hak Kyo Lee ),공홍식 ( Hoong Sik Kong ),이준호 ( Joon Ho Lee ) 한국동물자원과학회(구 한국축산학회) 2013 한국축산학회지 Vol.55 No.5

This study was conducted to investigate the basic concept of multiple trait effective daughter contribution(MTEDC) for dairy cattle sires and calculate effective daughter contribution(EDC) by applying a five lactation multiple trait model using milk yield test records of daughters for the Multiple-trait Across Country Evaluation(MACE). Milk yield data and pedigree information of 301,551 cows that were the progeny of 2,046 Korean and imported dairy bulls were collected from the National Agricultural Cooperative Federation and used in this study. For MTEDC approximation, the reliability of the breeding value was separated based on parents average, own yield deviation and mate adjusted progeny contribution. EDC was then calculated by lactation using these reliabilities. The average number of recorded daughters per sire by lactations were 140.57, 94.24, 55.14, 29.20 and 14.06 from the first to fifth lactation, respectively. However, the average EDC per sire by lactation using the five lactation multiple trait model was 113.49, 89.28, 73.56, 54.02 and 35.08 from the first to fifth lactation, respectively, while the decrease of EDC in late lactations was comparably lower than the average number of recorded daughters per sire. These findings indicate that the availability of daughters without late lactation records is increased by genetic correlation using the multiple trait model. Owing to the relatedness between the EDC and reliability of the estimated breeding value for sire, understanding the MTEDC algorithm and continuous monitoring of EDC is required for correct MACE application of the five lactation multiple trait model.

대구의 모바일 시험인증 현황 및 소개

조광현(Cho Kwang Hyun),오세일(Oh Se IL),이성훈(Lee Sung Hun) 한국통신학회 2013 한국통신학회 학술대회논문집 Vol.2013 No.6

영남지방 도축돈에 대한 폐렴발생 조사

조광현 ( Kwang Hyun Cho ),박인화 ( In Hwa Park ),도재철 ( Jae Cheul Do ),장성준 ( Sung Jun Chang ),박노찬 ( No Chan Park ),권헌일 ( Huon Il Kwon ),박덕상 ( Duk Sang Park ) 한국가축위생학회 1996 韓國家畜衛生學會誌 Vol.19 No.2

Lungs from 109 slaughter pigs with gross lesions indicating enzootic Pneumonia of pigs(EPP) and 16 grossly normal lungs, all originating from seven different herds, were subjected to microbiological examinations. The microbiological studies were included both bacterial and mycoplasmal culture. From lungs of 125 slaughter pigs, 87.2% pigs were pneumonia lesions alone or complexly. Mycoplasma spp., pasteurella multocida (P. multocida), Streptococcus spp., and Actinobacillus pleuropneumoniae(A. pleuropneumoniae) were detected in 39.4%, 42.2%, 13.8%, and 3.7% of the pneumonic lungs, respectively. P. multocida was the most frequently isolated organism in pneumonic lungs. Mycoplasmas not isolated organism in 33.9% the pneumonic lungs even if there are gross lessions mycoplasmas. The amounts of pneumonia in lungs with Mycoplasma spp. alone, a concurrence of Mvcoplasma spp. and P. multocida, P. multocida alone, a concurrence of P. multocida and A. pleuropneumoniae, and a concurrence of Mycoplasma spp. and A. pleuropneumoniae were 10.1%, 22.7%, 18.7%, 25%, and 30%, respectively. These findings indicated that P. multocida might be involved in the pathogenesis of pneumonia in slaughter pigs. Mycoplasma spp. was also, in this study, associated with higher frequency of pneumonia. The frequency of pigs snout lesion grade 0~5 inclusive were 27.2%, 28%, 19.2%, 16%, 6.4%, and 3.2% from 125 slaughter pigs. 32(25.6%) pigs were positive and 13~30% in the pigs from seven herds were found to be infected with atrophic rhintis(AR). A total of 46 P. multocida strains in pneumonic lungs were further characterized by capsular serotyping and testing for production of dermonecrotic toxin. 42(91.3%) of strains were capsular A and 4(8.7%) were type D. Out of the type A and type D strains, 86% and 75% were toxigenic, respectively.

멜라닌세포가 포함된 인공표피의 배양

조광현,이동윤,양성규,여운철,박경찬,윤재일 ( Kwang Hyun Cho,Dong Youn Lee,Seong Gyu Yang,Un Cheol Yeo,Kyoung Chan Park,Jai Il Youn ) 대한피부과학회 1997 대한피부과학회지 Vol.35 No.3

Background: Melanocytes grown in pure monolayer culure lack many of the cellular interactions that exist in vivo. This can be partially overcome by growing melanocytes together with other epidermal cells in skin equivalent models. Objective : The objective of the present study was to grow human melanocytes in human epidermis reconstructed on dermal substrates in vitro and to examine their response to UV radiation. Methods : The skin equivalents were prepared by seeding cultured human keratinocytes together with cultured human melanocytes(in a ratio of 5%) onto de-epidermized dermis. After 7 days of culture, they were exposed to UVB irradiation(total 150m J/cm over 5days). On day 12 of air exposure the sections of the skin equivalents were prepared for histology. The structure of the skin equivalents was studied following staining with hematoxylin and eosin. Melanocytes were characterized by DOPA staining and by immunohistochemistry. Results : Melanocytes were localized singly within the basal layer of the reconstructs. Melanin was also visible both in the melanocytes and in neighboring keratinocytes. There was an increase in melanocyte size and dendricity following UV irradiation. Melanocytes became positive to staining with HMB-45 antibody following UV irradiation. Conclusion : Our results indicate that melanocytes grown in reconstructed human epidermis are functional and capable of responding to UV irradiation. (Kor J Dermatol 1997;35(3): 526-531)

고성능 내후성강의 철도교 적용에 관한 연구

조광일(Cho, Kwang-Il),박찬희(Park, Chan-Hee),김종민(Kim, Jong-Min),박용명(Park, Yong Myung) 대한토목학회 2017 대한토목학회 학술대회 Vol.2017 No.10

파노라마 이미지 스티칭 기술을 이용한 3차원 얼굴 모델 텍스쳐링 기법

조광현(Kwang-Hyeon Cho),김계영(Gye-Young Kim),최형일(Hyung-Il Choi) 한국컴퓨터정보학회 2011 한국컴퓨터정보학회 학술발표논문집 Vol.19 No.1

본 논문에서는 사용자의 정면, 측면 영상을 이용하여 3차원 얼굴 모델에 적합한 텍스쳐 맵을 생성, 이를 이용하여 3차원 얼굴 모델을 생성하는 방법을 제안한다. 기존 3차원 얼굴 모델은 매핑 할 텍스쳐 영상들을 하나로 통합하여 이를 원통형 좌표계를 통해 텍스쳐링 하는 방법이 이용되고 있다. 이때 정면과 측면의 영상을 3차원 얼굴 모델의 중심축을 기준으로 계산하여 좌표에 맞게 투영시키고 통합 및 보간하여 텍스쳐링 하게 된다. 사용자는 이를 위하여 정면과 측면 영상을 원통형 좌표에 맞게 통합시키는 작업이 필요하다. 본 논문은 사용자에게 수반되는 이러한 작업을 줄이고 발생될 수 있는 텍스쳐의 왜곡을 최소화하기 위한 방법을 제시한다. 2차원 정면 측면 이미지를 3차원 얼굴모델에 투영시키고 정면과 측면을 구분하여 각 텍스쳐 간 경계 부분을 자연스럽게 처리하기 위해 파노라마 이미지 스티칭 기술을 이용하여 텍스쳐링 한 뒤 얼굴 모델을 생성하는 방법에 대해서 기술한다.