Blue mussel-derived antioxidant peptides PIIVYWK and FSVVPSPK exert osteogenic activities in mouse mesenchymal stem cells via MAPK-dependent BMP signaling pathway

제재영,형준호,윤순도,이창문 한국공업화학회 2016 한국공업화학회 연구논문 초록집 Vol.2016 No.1

Nowadays, a number of therapeutic agents for osteoporosis are available and focused on inactivation of osteoclasts. However, unfortunately the long-term use of anti-osteoporotic medications is associated with serious side effects. In this study, we examined osteogenic activities of blue mussel-derived antioxidant peptides PIIVYWK and FSVVPSPK in mouse mesenchymal stem cells (MSCs). Two antioxidant peptides increased osteogenic biomarkers such as alkaline phosphatase, type I collagen, osteocalcin and mineralization. Treatment antioxidant peptides in MSCs resulted in the increased expression of BMP-2, p-Smad1/5, Runx2, Dlx5, and osterix as well as phosphorylation of MAPKs. Further investigation using noggin, BMP antagonist, and MAPK inhibitors, we found that antioxidant peptides PIIVYWK and FSVVPSPK modulated osteoblast differentiation through ERK1/2 and JNK1/2-dependent BMP signaling pathway in MSCs.

모델 계에서의 진주담치 자숙 엑스분의 항산화 효과

제재영,최수진,박인규,안창범 한국식품영양과학회 2011 한국식품영양과학회 학술대회발표집 Vol.2011 No.10

Composition of Biologically Active Substances and Antioxidant Activity of New Zealand Deer Velvet Antler Extracts

제재영,박표잠,김은경,김현아,전병태,안창범,임동환 한국축산식품학회 2010 한국축산식품학회지 Vol.30 No.1

Deer velvet antler was subjected to the extraction process using boiling water at three different temperatures (100, 110 and 120℃) and 70% ethanol solution. Functional components such as uronic acid, sulfated-glycosaminoglycans (sulfated-GAGs) and sialic acid in the extracts were analyzed, and their antioxidant activities were investigated using several in vitro models. Uronic acid and sulfated-GAGs content of each extract significantly decreased with increasing extraction temperature (p<0.05), while the residues obtained from the upper and middle part of the antler had a higher uronic acid content than the residues obtained from the base section. Sialic acid contents were highest in compounds extracted at 110℃, followed by 120 and 100℃. The 70% ethanol extracts also had a high levels of uronic acid content, but not for sulfated-GAGs and sialic acid. All extracts showed good antioxidant ability in a dose-dependant manner, with the 100℃ residue exhibiting the strongest activity compared to the 110 and 120℃ extracts. In relation to the hydroxyl radical scavenging activity and reduction power, the 70% ethanol extract exhibited the strongest activity. Furthermore, the velvet antler extracts inhibited apoptosis in hydrogen peroxide-induced PC-12 cells.

Antimicrobial activity of chitosan-copper complexes against Vibrio vulnificus and Vibrio anguillarum

제재영 한국키틴키토산학회 2009 한국키틴키토산학회지 Vol.14 No.4

In this study, chitosan-copper complexes were synthesized with different ratio in order to improve its antimicrobial activity. Native chitosan and chitosan-copper complexes (5% chitosan-Cu2+ and 10% chitosan-Cu2+) were evaluated their antimicrobial effects against Vibrio vulnificus and Vibrio anguillarum, which gave rise to the outbreak of fish disease in aquacultural farms. Three kinds of samples suppressed the growth inhibition depending on incubation times and concentrations, and the activity was increased with increasing concentration of copper ions. Thermal stability experiment revealed that native chitosan and chitosan-copper complexes incubated at high temperatures showed excellent antimicrobial activity as compared to incubation at room temperature. The activity was also depending on inoculation times. These results indicate that chitosan-copper complexes would be used as antimicrobial agents against fish pathogenic bacteria such as vibrio species.

Prolyl Endopeptidase Inhibitory Activity of Sulfated Carboxymethyl Chitin

제재영,김세권,변희국 한국키틴키토산학회 2005 한국키틴키토산학회지 Vol.10 No.2

In order to improve the functional properties of chitin, sulfated carboxymethyl-chitin (SCM-chitin) was synthesized bychemical modifications. In addition, prolyl endopeptidase (PEP) inhibitory activity of SCM-chitin was investigated. SCM-chitinshowed noncompetitive with a substrate using Dixon plots, and the inhibition constant (Ki) of SCM-chitin was 8.0 mg/mL.

Antioxidant Peptide Isolated from Muscle Protein of Bullfrog, Rana catesbeiana Shaw

제재영,천충길,김세권 한국식품영양과학회 2007 Journal of medicinal food Vol.10 No.3

Bullfrog (Rana catesbeianaShaw) muscle protein was enzymatically hydrolyzed for extraction of an antiox-idant peptide. Antioxidant peptide from bullfrog muscle protein hydrolysate (APBMH) was purified using consecutive chro-matographic methods, and the amino acid sequence was identified as being Leu-Glu-Gln-Gln-Val-Asp-Asp-Leu-Glu-Gly-Ser-Leu-Glu-Gln-Glu-Lys-Lys (molecular mass of 1,988 Da) by quantitative time-of-flight electrospray ionization massspectroscopy. To assess antioxidant activities of APBMH, two different in vitrosystems were employed: free radical scav-enging activity by electron spin resonance (ESR) spectroscopy and polyunsaturated fatty acid (PUFA) peroxidation inhibitionassay. ESR revealed that APBMH is an effective free radial scavenger with activity similar to that of vitamin C against 1,1-diphenyl-2-picrylhydrazyl, hydroxyl, and superoxide radicals, and its 50% inhibitory concentration values were 179.4 .M,162.7 .M, and 176.1 .M, respectively. APBMH also significantly retarded PUFA oxidation, and more potently than did .-tocopherol, which was used as a positive control. In addition, the ability of APBMH to inhibit the oxidative damage of DNAwas assessed, in vitro, by measuring the conversion of supercoiled pBR322 plasmid DNA to the open circular form. It wasfound that APBMH significantly protected hydroxyl radical-induced DNA damage dose-dependently.

Antioxidant, Anti-acetylcholinesterase and Composition of Biochemical Components of Russian Deer Velvet Antler Extracts

제재영,박표잠,임동환,전병태,고강희,안창범 한국축산식품학회 2011 한국축산식품학회지 Vol.31 No.3

Russian deer velvet antlers were divided into three parts and subjected to a extraction process using hot water at 100, 110,and 120oC or an extraction with 70% ethanol. Each extract was analyzed for its biochemical components, including uronic acid, sulfated-glycosaminoglycans (sulfated-GAGs), and sialic acid, and the antioxidant and anti-acetylcholinesterase activities were investigated. Different levels of uronic acid and sulfated-GAGs were observed in the extracts according to the water temperature used for the extraction, and contents decreased with increasing extraction temperature. The upper layer of each extract showed high amounts of uronic acid and sulfated-GAGs, followed by the middle and base layers. Ethanol extraction was more effective for recovering uronic acid than sulfated-GAGs. Sialic acid content was the highest in the 110℃ extracts but was not observed in the ethanol extracts. Velvet antler extracts showed strong antioxidant activities against DPPH and hydrogen peroxide as well as strong reducing power in a dose-dependent manner. However, the antioxidant activities were different in each layer and according to the extraction method. Additionally, velvet antler extracts exhibited inhibitory activity against acetylcholinesterase, which is associated with Alzheimer’s disease, in a dose-dependent manner. These results suggest that velvet antler extracts are useful as a functional food ingredient and/or a pharmaceutical.

Free Radical Scavenging Activity of Enzymatic Hydrolysates of Hot Water Extract from the Shell of Reeve's Turtle (Chinemys reevesii)

제재영,김은경,박표잠,강미경,안창범 한국수산과학회 2008 Fisheries and Aquatic Sciences Vol.11 No.2

The shell of Reeve’s turtle has been used as a traditional folk medicine in Korea. We produced a hot water extract from Reeve’s turtle shell according to the traditional medical practice. To release bioactive peptides, the hot water extract was enzymatically hydrolyzed with various proteases, and the free radical scavenging activity of the hydrolysate was investigated against 1,1-diphenyl-2-picryl-hydrazyl (DPPH), hydroxyl and peroxyl radicals. The free radical scavenging activity of the enzymatic hydrolysates varied from 1 to 79% depending on the enzymes, free radical species, and concentration. The EC50 values demonstrated that the enzymatic hydrolysates of hot water extract from the shell of Reeve’s turtle are potential antioxidants.

Health Benefits of Chitosan-Phytochemical Conjugates

제재영 한국당과학회 2015 한국당과학회 학술대회 Vol.2015 No.02

갯가재의 식품성분

제재영(Jae Young JE),백지영(Ji Young BAEK),안창범(Chang Bum AHN) 전남대학교 수산과학연구소 2007 수산과학연구소논문집 Vol.16 No.-

수산학 상 중요 종의 하나인 갯가재의 식품학적 기초자료를 얻기 위해 함질소화합물, 지질 각 획분의 지방산, 무기질, 유기산 및 당을 분석하였다. 총유리아미노산 함량은 1,678.36±5.09 mg/100 g이었으며, sarcosine의 함량이 30.83%로 매우 높은 것이 특징적이었고, 다음으로 arginine (20.22%), taurine(8.69%), glycine (6.43%) 및 alanine (5.01%)의 순으로 그 함량이 높았으며, 이들 아미노산이 전 유리아미노산의 약 71%를 차지하였다. 핵산관련물질은 inosine과 IMP의 함량이 각각 38.22±2.68 mg/ 100 g 및 16.82±1.93 mg/ 100 g으로 높았으며 ATP의 함량이 가장 낮았다. 총엑스분질소 함량은 516.36 mg/100g이었으며 이 중 유리아미노산 질소가 314.85mg/100 g로 가장 많은 비율을 차지하였고, 다음으로 총크레아티닌 질소, betaine 질소, 암모니아 질소의 순이었다. 유기산은 총 모두 8종이 동정되었으며, acetic acid, lactic acid 및 citric acid가 각각 195.65±4.24, 81.55±2.96 및 40.97±0.93 mg/100 g으로 그 함량이 많았다. 당은 glucose와 arabinose가 동정되었으며 각각 71.09±2.37 및 1.03±0.04 mg/100 g을 차지하고 있었다. 총지질과 인지질의 주요 구성지방산은 C22:6, C20:5, C23:0, C18:2 및 C16:0이었으며, 폴리엔산의 함량이 약 80% 이상으로 매우 높은 것이 특징적이었고, 폴리엔산 중에서도 C22:6 및 C20:5가 대부분을 차지하였다. 모노엔산의 함량은 가장 낮았는데 이 같은 경향은 중성, 인 및 당지질의 경우도 마찬가지였다. 중성 및 당지질의 주요 구성지방산은 C20:5, C22:6, C18:3n-6, C23:0 및 C16:0이었는데 총지질이나 인지질에 비해 C18:3n-6의 함량이 높고, C22:6 및 C20:5는 상당히 낮았다. 당지질의 경우는 다른 어느 지질획분에서보다 폴리엔산의 함량이 낮고 포화산의 함량이 높았으며, 특히 홀수 지방산인 C23:0의 함량이 매우 높았고, C18:3n-6의 함량이 높은 반면 EPA의 함량이 특히 낮았다. 무기질 중에서는 Na와 K가 각각 433.0±5.4 및 276.5±4.6 mg/100 g으로 많은 양을 차지하고 있었다. 중금속 중에서는 Zn이 0.81±0.02 mg/100 g으로 그 함량이 높았다. Nitrogenous compounds (free amino acids, nucleotides and their related compounds, total creatinine, betaine, TMAO, and TMA), non-volatile compounds (sugars and organic acids), composition of fatty acid of lipid, and minerals in the edible mantis shrimp (Oratosquilla oratoria) were investigated. The content of total free amino acids was 1,678.36 mg/100 g and the predominant amino acids were sarcosine, arginine, taurine, glycine and alanine. These amino acids accounted for 71% of total free amino acids. Among the nucleotides and their related compounds, inosine was the major component, holding 38.22±2.68 mg/100 g. Free amino acid-N was the most abundant, resulting 60.97% of extractive nitrogen which was 314.85 mg/100 g and next total creatinine-N, betaine-N, and ammonia-N in order. In the non-nitrogenous compounds, acetic acid, lactic acid, and citric acid were the major portion of the 8 kinds of organic acids detected and glucose had the highest content of the 2 kinds of sugar detected. The predominant minerals were Na and K, which comprised 433.0±5.4 and 276.5±4.6 mg/100 g, respectively. The major fatty acids were C22:6, C20:5, C23:0, C18:2, and C16:0 in total lipid and phospholipid and C20:5, C22:6, C18:3n-6, C23:0, and C16:0 in neutral lipid and glycolipid. The C22:6 content of the neutral lipid and glycolipid was much lower than that of the total lipid and phospholipid. The content of monounsaturated fatty acids was the lowest in all lipid fraction. In glycolipid, C23:0 was even higher compared to that of any other lipid fraction, but the content of C20:5 was lower.