GA3처리와 저온습윤처리에 의한 흑노호(Kadsura coccinea)의 종자발아 및 유묘생장 촉진

제병일,전중석,강점순,최영환 한국환경과학회 2023 한국환경과학회지 Vol.32 No.1

Kadsura coccinea (Lem.) A.C. Smith is used as a medicinal plant and cosmetic material in China and Southeast Asia. To mass-produce Kadsura coccinea seedlings, the effects of gibberellic acid (GA3) and cold stratification treatments on seed germination were investigated. Seed germination rate with GA3 treatment was most effective at concentrations of 250 or 500 mg/L. With respect to mean germination time (MGT), mean daily germination, and T50 (days to reach 50% seed germination), the germination-promoting effect was improved as the concentration of GA3 increased. Stem growth of seedlings was the highest following GA3 treatments of 250 and 500 mg/L, and the growth promoting effect gradually decreased as the concentration of GA3 decreased. Root growth was stimulated at GA3 concentrations of 250–1,000 mg/L. Examination of the effect of stratification treatment for 15, 30 and 60 days at temperatures of 0, 5 and 10℃ on the germination rate revealed that the most stratification treatment temperature was 10℃, and the results improved with longer treatment periods. Altogether, GA3 and stratification treatments improved the seed germination rate, shortened the MGT, improved germination uniformity, and produced healthy seedlings.

Effect of storage temperature on chilling injury of ‘Cheongdobansi’, an astringent persimmon of Korea

남현진,제병일,이용재 한국원예학회 2023 Horticulture, Environment, and Biotechnology Vol.64 No.6

Low-temperature storage is used to extend the postharvest storage of horticultural produce. However, during low-temperature storage, certain fruit exhibit physiological disorders and alterations, which is known as chilling injury (CI). Nevertheless, the cause and the details of CI symptoms are unclear. Here we report ‘Cheongdobansi’, an astringent persimmon that showed a pattern of CI development, initiating in the middle of the inner fl esh in contact with white pith, spreading along the vascular tissue, and ending by covering the upper mesocarp. CI critical temperature of ‘Cheongdobansi’ persimmon was 8 the maximum CI induction was at 5 °C. However, temperature showed very low CI induction at −1 and , indicating that −1 is the optimal storage temperature of ‘Cheongdobansi’ persimmon. This result aslo challenges the common idea of CI that lowering the temperature below the critical temperature worsens CI symptoms. Interestingly, the highest ethylene produc tion was detected at 2 °C, with very low occurrence of decay at this temperature, suggesting a new hypothesis that ethylene production might be involved in the inhibition of decay. Moreover, the increase in respiration and decrease in fi rmness of persimmons take precedence over the prominent CI symptoms, where jelly-like fl esh formation and electrolyte leakage occur. Electrolyte leakage showed a signifi cant increase after the onset of visible CI symptoms. Our results provide important clues, such as the correlation between the structure of vascular tissue and CI development in fruit, and between ethylene and CI decay, which are very useful for post-harvest storage of astringent persimmons for agricultural use.

Reprogramming of the Activity of the Activator/Dissociation Transposon Family during Plant Regeneration in Rice

김철민,제병일,Hai Long Piao,Soon Ju Park,김민정,Sung Han Park,Jin Young Park,Su Hyun Park,Eun Kyeong Lee,Nam Soo Chon,Yong Jae Won,Gi Hwan Lee,Min Hee Nam,Doh Won Yun,Myung Chul Lee,Young Soon Cha,이곤호,은무영,한창덕 한국분자세포생물학회 2002 Molecules and cells Vol.14 No.2

Many aspects of epigenetic phenomena have been elu-cidated via studies of transposable elements. An active transposable element frequently loses its ability to mobilize and goes into an inactive state during devel-opment. In this study, we describe the cyclic activity of a maize transposable element dissociation (Ds) in rice. In rice genome, Ds undergoes the spontaneous loss of mobility. However, an inactive state of Ds can be changed into an active state during tissue culture. The recovery of mobility accompanies not only changes in the methylation patterns of the terminal region of Ds, but also alteration in the steady state level of the acti-vator (Ac) mRNA that is expressed by a constitutive CaMV 35S promoter. Furthermore, the Ds-reactiva-tion process is not random, but stage-specific during plantlet regeneration. Our findings have expanded previous observations on Ac reactivation in the tissue culture of maize.

하위 적엽처리가 딸기의 생육 및 생산성에 미치는 영향

이규빈,이정은,제병일,이용재,박영훈,최영환,손병구,강남준,강점순,Lee, Gyu-Bin,Lee, Jung-Eun,Je, Byoung-Il,Lee, Yong-Jae,Park, Young-Hoon,Choi, Young-Whan,Son, Beung-Gu,Kang, Nam-Jun,Kang, Jum-Soon 한국환경과학회 2020 한국환경과학회지 Vol.29 No.3

The purpose of this study was to investigate the effects of defoliation treatment on the growth and yield of strawberries. There was a remarkable growth in the above-ground part and root of untreated strawberry plants possibly due to higher amount of photosynthesis, while overall plant growth was suppressed as the level of defoliation treatment increased. In both the "Seolhyang" and "Maehyang" cultivars examined, defoliation treatment resulted in small fruits and a low number of fruits per plant. Notably, 50% defoliation significantly reduced the number of fruits per plant to 8.2, compared to 13.8 in untreated plants. Defoliation treatment also negatively influenced the fruit quality including color, sugar content, and solid-acid rate. However, no significant changes in fruit firmness was observed in either cultivar. Therefore, retaining enough leaves without defoliation treatment can be important to increasing fruit yield, producing high quality fruits and saving labor required for defoliation.

딸기의 주두 제거와 옥신 처리가 딸기의 과형 및 품질에 미치는 영향

이정은,이규빈,제병일,이용재,박영훈,최영환,손병구,강남준,강점순 (사) 한국생물환경조절학회 2019 생물환경조절학회지 Vol.28 No.1

The effects of stigma removal from floral organ and treatment of auxin on those regions were evaluated on the fruit growth and quality in strawberry (Fragaria ananassa Duch.). The removal of stigma reduced the fruit weight, fruit length, and fruit width, while fruit growth declined as the number of removed stigma increased. The removal of stigma also resulted in reduced fruit firmness, sugar content, and total anthocyanin content, and decreased value of Hunter a for red color and increased value of Hunter b for yellow. The number of seeds deceased as the regions of removed stigma increased. The treatment of auxin on the region of removed stigma improved fruit size, while it reduced fruit firmness, sugar content, and anthocyanin content. The delay in fruit ripening was observed from both stigma removal and auxin treatment. The incidence of malformed fruits increased as the regions with removed stigma increased. The treatment of auxin also promoted fruit malformation. 본 연구는 딸기의 화기에서 주두를 제거하고 주두를제거한 부위에 옥신제인 토마토톤을 처리하여 과실의 생육 및 품질을 검토하였다. 화기에서 주두를 제거함으로써 과중, 과장, 과폭이 낮아졌으며, 주두를 제거하는 부위가 많을수록 과실의 생육이 저하되었다. 또한 화기에서 주두를 제거하면 과실의 경도와 당도가 낮아졌다. 딸기에서 주두를 제거하면 붉은색을 나타내는 Hunter a값이 낮아지고 황색을 띄는 b값이 높아졌으며, 과실의 총안토시아닌 함량도 감소하였다. 또한 주두를 제거하는부위가 많을수록 과실당 종자수가 감소하였다. 주두를제거한 부위에 옥신제인 토마토톤을 처리하면 과실의 크기가 커졌다. 반면 토마토톤을 처리는 대조구에 비해 과실의 경도, 당도 및 안토시아닌 함량은 낮아졌다. 딸기에서 주두를 제거하거나 토마토톤을 처리함으로써 과실의 숙성이 지연되었다. 또한 제거하는 주두부위가 많을수록 기형과가 증가하였고, 토마토톤 처리에 의해서도기형과 발생율은 증가하였다. 따라서 딸기에서 토마토톤사용은 과실의 크기를 증가시키지만 숙성을 지연시키고기형과 발생률을 증가시켰다.

발아온도 및 Spermine, Putrescine이 딸기의 화분 발아에 미치는 영향

이정은,김현도,제병일,이용재,박영훈,최영환,손병구,강남준,강점순,Lee, Jung-Eun,Kim, Hyeon-Do,Je, Byoung-Il,Lee, Yong-Jae,Park, Young-Hoon,Choi, Young-Whan,Son, Beung-Gu,Kang, Nam-Jun,Kang, Jum-Soon 한국환경과학회 2020 한국환경과학회지 Vol.29 No.4

This study was conducted to determine the optimum conditions for the treatment of polyamine to promote pollen germination and improve the fruiting rate by overcoming fertilization defects in low-temperature strawberry cultivation. The optimum temperature for pollen germination of strawberries was 30 ℃ for Seolhyang and 25 ℃ for Maehyang, and Seolhyang had a higher pollen germination rate than that of Maehyang. The addition of polyamines, namely spermine and putrescine to the medium improved the pollen germination rate and spermine treatment showed a better effect than putrescine treatment. The proper polyamine type and treatment concentration for enhancing the potency of pollen germination was 500 μM of spermine for both Seolhyang and Maehyang, which improved the pollen germination rate by 19-23% compared with that of the control. However, combined treatment of spermine and putrescine, resulted in a lower germination rate lower than that of the single treatment. Our results indicated that the treatment of polyamines during flowering in protected cultivation of strawberrise can improve the fruiting rate by overcoming the problem of poor pollen germination due to low temperature.

광부족 조건이 딸기의 생육 및 생산성에 미치는 영향

이규빈,이정은,제병일,이용재,박영훈,최영환,손병구,강남준,강점순,Lee, Gyu-Bin,Lee, Jung-Eun,Je, Byoung-Il,Lee, Yong-Jae,Park, Young-Hoon,Choi, Young-Whan,Son, Beung-Gu,Kang, Nam-Jun,Kang, Jum-Soon 한국환경과학회 2020 한국환경과학회지 Vol.29 No.2

The purpose of this study was to investigate the effect of shading on the growth and productivity of strawberries. Photosynthesis was normally achieved under natural light without shading treatment, and vegetative growth of under- and aboveground part of strawberry plants were excellent. Strawberry fruit productivity and quality were different depending on shading conditions. In natural light, they were improved possibly by balanced vegetative and reproductive growth. However, under light-shading conditions with insufficient sunshine, photosynthetic activity deteriorated and carbohydrate production was therfore inadequate; this adversely affected plant height and quality. The negative effects were more pronounced at 50% shading condition. Shading treatments resulting in insufficient sunshine had a detrimental impact on plant productivity and growth; this implies that proper shading conditions could heip improve yield and fruit quality.

필름코팅 소재들이 배추 종자의 발아 및 활력에 미치는 영향

강점순,김현도,이정은,제병일,이용재,박영훈,최영환 한국환경과학회 2021 한국환경과학회지 Vol.30 No.12

The purpose of this study was to identify for extenders, adhesives, colorants, and fungicides suitable for the production of high-quality film-coated seeds of Chinese cabbage. In addition, the effect of various additives used for film coating on the germination of coated seeds and seedling vigor was investigated to establish appropriate treatment conditions for film coating efficiency. The germination rate and germination speed in Chinese cabbage film-coated seeds did not differ significantly depending on the type and concentration of the extender, while the use of Talc resulted in superior granulation capacity and adhesion of the seed coat in comparison to Zeolite. There was no significant difference in the germination rate depending on the type of adhesive used for film coating of Chinese cabbage seeds, but the germination rate was slightly slower in the film-coated seeds using carboxymethyl cellulose as an adhesive. On the other hand, the seeds coated with a film using 1% PVA as an adhesive not only maintained hardness enough to withstand external pressure, but also showed a high germination rate. In addition, the suitable disinfectant for film coating was venoram, and the treatment concentration was good at 250 mg·L-1. The type of colorant used in the film-coated seeds of Chinese cabbage did not significantly affect the germination rate, germination speed, and seedling vigor. The seeds film-coated using the liquid colorant were superior in appearance to the seeds film-coated with the solid colorant, which implies the possibility of reduction in the manufacturing costs by using the water-soluble paints as colorants for film-coated seeds.

수박종자의 건열 처리가 발아 및 유묘생장에 미치는 효과

박은지,이정은,안성광,제병일,박영훈,이용재,최영환,강점순 한국환경과학회 2023 한국환경과학회지 Vol.32 No.2

This study was conducted to investigate changes in seed vigor based on temperature of dry heat and duration treatment of watermelon seeds and examine the effect on percent of emergence and seedling vigor. When the upper limit temperature of dry heat treatment was raised to 80℃, the percent of the germination decreased. Moreover, T50 was delayed as the upper limit temperature of dry heat treatment increased. The higher the upper limit temperature of dry heat treatment and the longer the treatment period, the higher the percentage of abnormal seedlings. The optimum upper limit temperature for dry heat treatment was 72℃, and the treatment period was five days. Seed vigor was better maintained at 30℃, 45℃, and 52℃, followed by stepwise exposure to high temperatures of 72℃, the upper limit of dry heat treatment, rather than dry heat treatment at a high temperature of 72℃ for 5 days from the initial stage of treatment. When the fungicide was added during the dry heat treatment process, the germination percentage decreased and the percent of the abnormal seedling percentage increased. However, the addition of 10 mg/kg fungicide did not significantly reduce seed vigor.

수박 엘리트 계통의 GBS를 통한 마커이용 육종용 SNP 마커 개발

이준우,손병구,최영환,강점순,이용재,제병일,박영훈,Lee, Junewoo,Son, Beunggu,Choi, Youngwhan,Kang, Jumsoon,Lee, Youngjae,Je, Byoung Il,Park, Younghoon 한국식물생명공학회 2018 식물생명공학회지 Vol.45 No.3

본 연구는 국내 육종 회사에서 개발된 수박(Citrullus lanatus L.) 우량 육성계통 20종을 대상으로 Genotyping-by-sequencing(GBS) 분석을 통해 품종식별, 순도검정, 그리고 마커이용여교잡(Marker-assisted backcross, MABC)용 SNP 세트를 개발하고자 수행되었다. GBS 분석 결과 총 1,100,000천개 raw read 중 77%가 수박 유전체에 mapping되었으며 평균 mapping region은 약 4,000 Kb로 2.3%의 genome coverage를 보였다. Filtering을 통해 평균 depth 31.57의 SNP 총 2,670개를 얻었으며, 20개 계통에 대한 이들의 Polymorphic information content(PIC) 값의 범위는 0.1 ~ 0.38 였다. 이 중 PIC 값이0.3이상이며 각 염색체 별로 5개씩 균등히 분포된 SNP 총 55개를 최종 선발하였다. 사용된 20개 계통의 유연관계분석을 위해 선발된 55개 SNP를 기반으로 한 주성분 분석(Principle component analysis, PCA) 결과 주성분 1 (52%)과 주성분 2 (11%)를 기준으로 4개의 그룹으로 분류 되었으며 각 계통 간 유전자형에 따른 뚜렷한 식별이 가능하였다. 계층적 군집화(Hierarchical clustering) 분석에서도PCA에서와 유사한 분류양상을 관찰할 수 있었다. 따라서 본 연구에서 개발된 SNP 세트는 적용 가능성이 검증된 20개 계통뿐 만 아니라 향후 다양한 수박 육종소재 및 품종에 대한 품종식별, F1 순도검정 및 MABC에 활용될 수 있으리라 기대된다. This study was conducted to develop an SNP set that can be useful for marker-assisted breeding (MAB) in watermelon (Citrullus. lanatus L) using Genotyping-by-sequencing (GBS) analysis of 20 commercial elite watermelon inbreds. The result of GBS showed that 77% of approximately 1.1 billion raw reads were mapped on the watermelon genome with an average mapping region of about 4,000 Kb, which indicated genome coverage of 2.3%. After the filtering process, a total of 2,670 SNPs with an average depth of 31.57 and the PIC (Polymorphic Information Content) value of 0.1~0.38 for 20 elite inbreds were obtained. Among those SNPs, 55 SNPs (5 SNPs per chromosome that are equally distributed on each chromosome) were selected. For the understanding genetic relationship of 20 elite inbreds, PCA (Principal Component Analysis) was carried out with 55 SNPs, which resulted in the classification of inbreds into 4 groups based on PC1 (52%) and PC2 (11%), thus causing differentiation between the inbreds. A similar classification pattern for PCA was observed from hierarchical clustering analysis. The SNP set developed in this study has the potential for application to cultivar identification, F1 seed purity test, and marker-assisted backcross (MABC) not only for 20 elite inbreds but also for diverse resources for watermelon breeding.