음양곽(淫羊藿) 열수 추출물의 Smad 신호 억제를 통한 간성상세포의 활성 조절

정지윤,민병구,박정아,변성희,조일제,김상찬,Jung, Ji Yun,Min, Byung-Gu,Park, Chung A,Byun, Sung Hui,Cho, Il Je,Kim, Sang Chan 대한한의학방제학회 2018 大韓韓醫學方劑學會誌 Vol.26 No.3

Objectives : In Traditional Korean Medicine, Epimedium koreanum Nakai has diverse pharmacological activities to treat impotence, forgetfulness, cataract and exophthalmos. Present study investigated anti-fibrogenic effects of E. koreanum water extract (EKE) in hepatic stellate cells (HSCs). Methods : To study anti-fibrogenic effects of EKE, LX-2 cells, a human immortalized HSCs, were pre-treated with $3-300{\mu}g/mL$ of EKE, and then subsequently exposed to 5 ng/mL of transforming growth $factor-{\beta}1$ ($TGF-{\beta}1$). Expression level of ${\alpha}-smooth$ muscle actin was determined by immunoblot analysis. Phosphorylation of Smad, transactivation of Smad, and expression of plasminogen activator inhibitor-1 (PAI-1) were monitored to investigate the effect of EKE on $TGF-{\beta}1-mediated$ signaling pathway. Results : Up to $100{\mu}g/mL$, EKE did not show any cytotoxicity on LX-2 cells. Pre-treatment of EKE ($100{\mu}g/mL$) significantly inhibited ${\alpha}-smooth$ muscle actin expression induced by $TGF-{\beta}1$. In addition, EKE significantly decreased Smad2 and Smad3 phosphorylations, Smad binding element-driven luciferase activity and PAI-1 expression by $TGF-{\beta}1$. Of three flavonoid compounds found in EKE, only quercertin ($30{\mu}M$) attenuated $TGF-{\beta}1-mediated$ PAI-1 expression. Conclusion : These results suggest that EKE has an ability to suppress fibrogenic process in HSCs via inhibition of $TGF-{\beta}1/Smad$ signaling pathway.

실험동물에서의 환경호르몬 물질의 생체내 영향 및 검색법 정립에 대한 연구

정지윤,이영순,Jung, Ji-Youn,Lee, Yong-Soon 대한수의학회 2005 大韓獸醫學會誌 Vol.45 No.4

The major protocol features of the rodent uterotrophic assay have been evaluated using a range of reference chemicals. The protocol variables considered include the selection of the test species and route of chemical administration, the age of the test animals, the maintenance diet used, and the specificity of the assay for estrogens. The rodents were ovariectomized under general anesthesia via bilateral flank incisions and randomly assigned to groups of 5 animals. Chemicals were DEHP, DBP, BPA and NP, were injected sc once daily with combinations of chemicals treatments for 3 days. In the results, the reported estrogenic chemicals DEHP and DBP were both negative in the single dose treatments. But, in the combinations of chemicals treatments, DEHP and DBP increased in bud number of mammary gland. Treatment of ovariectomized mice with combinations of other chemicals resulted in uterine and vaginal hyperplasia. The additive estrogenic effects were seen with the combinations of $17{\beta}$-Bestradiol and DBP treatment. the competitive estrogenic effects were seen with the combinations of $17{\beta}$-Bestradiol and nonylphenol, $17{\beta}$-Bestradiol and bisphenol-A treatments. These results offers a sysmatic and mechanistically informative approach to assessing estrogenicity. it provides a useful profile of activity using a reasonable amount of resources and is compatible with the study of individual chemicals as well as the investigation of interactions among combinations of chemicals. The results described illustrate the intrinsic complexity of evaluating chemicals for estrogenic activities and conform the need for rigorous attention to experimental design and criteria for assessing estrogenic activity.

면역기능 증강 신물질에 대한 마우스의 면역학적 및 혈액학적 변화

정지윤,Jung, Ji-Youn 대한수의학회 2005 大韓獸醫學會誌 Vol.45 No.4

Immu-Forte composed of chitosan, ${\beta}-glucan$, manno-oligosaccharide and pangamic acid was evaluated for its effectiveness as a nonspecific immunostimulator in mice. The effects of Immu-Forte were determined by analysis of cytokines using ELISA and phenotype of leukocyte subpopulations using monoclonal antibodies specific to mouse leukocyte differentiation antigens and flow cytometry. All T cells, all B cells, CD4 T cells, CD8 T cells, macrophages, IL-2, IL-4, IL-12 and IFN-r in Immu-Forte A-treated group increased in 1 months posttreatment and were significantly higher (p < 0.05) than that of control at 1 months posttreatment. All T cells, all B cells, CD4 T cells, CD8 T cells, macrophages and IL-2 in Immu-Forte EX-treated low and middle dose groups increased in 1 months posttreatment and were significantly higher (p < 0.05) than that of control at 1 months posttreatment. In the Immu-Forte soybean-treated group, NK cells and IL-4 were significantly higher in middle dose-treated group, and IL-2, IL-4 and IFN-r were significantly higher in low dose-treated group. In the Immu-Forte F-treated group, all T cells, all B cells, CD4 T cells, CD8 T cells, macrophages, NK cells, IL-2, IL-4, IL-12 and IFN-r in high dose-treated group and all T cells, all B cells, CD4 T cells, CD8 T cells, macrophages, IL-2, IL-4, IL-12 and IFN-r in middle dose-treated group and NK cells, IL-2, IL-4, IL-12 and IFN-r in low dose-treated group were significantly higher (p < 0.05) than that of control at 1 months posttreatment. In conclusion, this study has demonstrated that Immu-Forte had an immunostimulatory effect on mice through proliferation and activation of mouse immune cells.

Intralipidos에 대한 변이원성시험

정지윤,이원우,임종희,남정석,제정환,이광훈,강병철,이병희,박재학,이영순,Jung, Ji-Youn,Lee, Won-Woo,Ihm, Jong-Hee,Nam, Jeong-Seok,Che, Jeong-Hwan,Li, Guang-Xun,Kang, Byeong-Cheol,Yi, Beoung-Hi,Park, Jae-Hak,Lee, Yong-Soon 한국독성학회 1998 Toxicological Research Vol.14 No.3

In order to evaluate the mutagenic potential of Intralipidos produced by Greenmate cooperation. We performed Salmonella typhimurium reversion assay, chromosomal aberration test on chinese hamster ovarian cells and in vivo micronucleus assay using mouse bone marrow cells. In the reverse mutation test using Salmonella typhimurium TA98 and TA100, Intralipidos did not increase the number of revertant at any of the concentration tested in this study. Intralipidos did not increase the number of cells having structural or numberical chromosome aberration in cytogenetic test. In mouse micronucleus test, no significant increase were observed in the occurrence of micornucleated polychromatic erythrocytes in ICR male mice intraperitoneally administered with Intralipidos. These results indicate that Intralipidos has no genetic toxicity under these experimental conditions.

유방의 세침흡인 세포검사: 수기 액상세포검사법(Liqui-$PREP^{TM}$)과 고식적 도말법과의 비교

정지윤,김정식,김영수,김혜정,박지영,Jeong, Ji-Yun,Kim, Jeong-Shik,Kim, Young-Su,Kim, Hye-Jung,Park, Ji-Young 대한세포병리학회 2008 대한세포병리학회지 Vol.19 No.1

Fine needle aspiration (FNA) cytology of the breast is a useful method for diagnosing breast lesions. Yet making the definite diagnosis with performing FNA is limited by some problems, such as the low cellularity, the poor preservation and the obscuring background. Recent studies have found that liquid-based cytology solves such problems, but it is an expensive method and it is limited by the loss of the background information. The purpose of this study is to compare the Liqui-$PREP^{TM}$, a new manual liquid-based method of cytology, and the conventional smears for analyzing breast FNA cytology materials. A total of 31 randomized FNA specimens of breast were studied. In each case, both the conventional smears and the Liqui-$PREP^{TM}$ method were performed, and the smears were evaluated for cellularity, cellular preservation, the background, the cytologic features and the architectural arrangement. The cellularity and architectural arrangement were equal for both preparations. The Liqui-$PREP^{TM}$ specimens showed better cellular preservation, loss of the obscuring background, no overlapping of cells and a smaller area to screen compared with the conventional smears. Moreover, it has the potential advantages of being able to use the remaining specimens for immunohistochemical study and ploidy analysis, and it can reduce the costs for preparation compared with the other liquid-based methods of cytology. But some background information is lost in the Liqui-$PREP^{TM}$ specimens, the same as the other liquid-based methods of cytology. In conclusion, the Liqui-$PREP^{TM}$ and conventional smears showed good correlation, but they have their respective advantages and disadvantages. These results suggest that Liqui-$PREP^{TM}$ can contribute to making the accurate diagnosis with performing breast FNA cytology when it is used along with other methods.

경도지적장애인의 마음읽기와 언어발달에 관한 연구

정지윤 ( Ji Yun Jung ),김화수 ( Wha Soo Kim ),이근용 ( Kun Youg Rhee ) 한국특수교육문제연구소 2014 특수교육저널 : 이론과 실천 Vol.15 No.3

경도지적장애인의 마음읽기와 언어발달의 관계를 알아보기 위하여 경도지적장애인 20명과경도지적장애 집단과 생활연령 및 언어연령을 일치시킨 각 20명을 대상으로 마음이론 과제를 실시하였다. 각 집단의 마음이론 과제 평균을 비교하였으며, 경도지적장애 집단 내의 마음이론 과제 점수와 언어발달 사이의 상관을 알아보았다. 연구 결과, 일차순위 마음이론 과제 수행에서는 경도지적장애 집단과 언어연령일치 집단, 경도지적장애 집단과 생활연령일치 집단 사이에 유의미한 차이가 나타났다. 이차순위 마음이론 과제 수행에는 경도지적장애 집단과 생활연령일치 집단 사이에 의미 있는 차이가 나타났다. 경도지적장애 집단 내의 일차순위 마음 이론 과제의 수행은 BASA 빈칸 채우기와 유의미한 상관을 나타내었고, 이차순위 마음이론 과제 수행에는 BASA 읽기, BASA 빈칸 채우기, 수용어휘력에서 유의미한 상관을 나타내었다. 본 연구는 마음이론과제를 동영상으로 제작하여 의사소통상황에서 마음이해를 통한 언어적 접근의 필요성을 제시하였다. The purpose of this study is to investigate relationship of mind reading and language development. This study was conducted about 20 adult with mild intellectual disability, 20 general people in the chronological age matched group, and 20 general people in the language age matched group. All theory of mind task performance was compared with mild intellectual disability group (MA), chronological-age (CA) matched group and language-age (LA) matched group. And, language development and theory of mind within MA groups’ correlations were examined. The results of this study were as follows: First, a one way ANOVA was carried out comparing the three groups (MA, CA and LA) with respect to their scores on first-order theory of mind task. The main effect for group was significant. Second, the result of analysis showed that there was statistically significant difference in MA vs. CA groups on the second-order theory of mind task. Third, mild intellectual disabilities within the group in the language test and correlated with theory of mind task performance. The findings are discussed in terms of the specificity of the intellectually disabled group. Therefore the result of this study could give suggestion for intervention and understanding of adult with mild intellectual disability.

건전한 카지노 문화의 정착을 위한 도박중독과 충동성에 관한 이해

정지윤(Ji Yun Jung),황현국(Hyun-kuk Hwang) 한국엔터테인먼트산업학회 2016 한국엔터테인먼트산업학회 학술대회 논문집 Vol.2016 No.5

본 연구는 도박 고위험군을 미리 파악하고자 정상적이라 여겨지는 대학생 집단을 대상으로 충동성과 관련된 성격유형의 특징이 도박에 미치는 영향을 알아보고, 그리고 도박집착이 조절실패를 거쳐 궁극적으로 중요관계손상에 미치는 영향관계를 조사하였다. 실증분석에 따른 본 연구의 결론 및 시사점은 다음과 같다. 첫째, 인지충동성, 운동충동성 및 무계획충동성으로 구분된 충동성의 특징들은 도박집착과의 영향관계에서 유의한 영향을 미치지 못하는 것으로 나타났다. 이러한 분석결과는 충동성의 성격특성이 도박집착이라는 도박행위과정에 뚜렷한 영향을 미치는 요인이 될 수 없다는 것을 시사한다. 둘째, 도박행위과정에서 도박집착이 도박조절실패에 미치는 영향력은 39.4%이었고 유의적인 영향을 미치는 것으로 나타났다. 이러한 분석결과는 도박행위가 반복되거나 습관화될 경우, 결과적으로 통제력상실과 같은 도박중독의 경과를 보여줄 수 있음을 시사한다. 셋째, 도박행위과정에서 도박집착 및 도박조절실패가 중요관계손상에 미치는 직접효과는 각각 33.1%, 19.4%로 유의적인 영향을 미치는 것으로 나타났다. 또한 도박집착이 도박조절실패를 통해 중요관계손상에 미치는 간접효과는 7.6%로 나타나 중요관계손상에 대한 도박집착의 영향력은 총 40.8% 이었다. 이러한 분석결과는 도박집착이 직접적으로, 또는 통제력상실과 같은 도박중독의 과정을 거쳐 중요관계로의 손상으로 이어질 수 있음을 시사한다. 따라서 본 연구결과는 도박중독의 위험성뿐 아니라 도박의 폐해로 고통을 경험할 가능성이 높은 습관적 또는 장기 카지노 출입고객에 대해서는 캠페인 또는 중독관련 치료프로그램을 통해 도박 문제에 대한 심각성을 일깨우고, 변화를 유도할 필요성을 제기하고 있다.

지리정보시스템(GIS)을 활용한 지역사회 청소년정책 인프라 분석

정지윤(Jung, Jiyun) 한국청소년정책연구원 2020 한국청소년연구 Vol.31 No.2

본 연구는 지역사회 청소년정책 인프라의 분포와 지역별 차이를 확인하고자 수행하였다. 지역사회는 전국 228개 시군구이며, 청소년정책 인프라는 청소년조례, 청소년정책예산, 청소년정책전달체계이다. 이는 모두 행정구역을 단위로 집계되는 데이터라는 점을 감안하여 지리정보시스템을 활용하여 분포분석과 공간자기상관분석, LISA 분석을 수행하였다. 분석결과, 조례, 예산, 전달체계 모두 지역별 편차가 매우 큰 것을 알 수 있었다. 조례의 경우 1개에서 12개 사이의 분포를 보였으며, 예산은 전체 예산대비 청소년정책예산으로 확인한 결과 0.0%∼4.1%의 분포가 나타났고, 이 중 전체예산대비 청소년정책예산이 0.000%이하인 지역도 있는 것을 확인할 수 있었다. 마지막으로 전달체계는 1개에서 최대 65개의 분포를 보였고, 시설의 지역별 집중화정도를 확인하기 위해 입지계수를 분석한 결과 0.2에서 17.4까지 큰 폭을 보였다. 또한 모란지수가 모두 0보다 크므로 청소년정책 인프라는 특정지역이 증가하면 주변지역의 인프라도 증가하는 양의 공간적 자기상관성을 보였고, 각 요인들은 공간요인을 고려하여 분석함이 타당한 것으로 확인되었다. 결과를 토대로 지역별 편차를 줄여 청소년정책 인프라의 공평성과 평등성을 확보하기 위한 방안을 제안하였다. 주요 내용은 지역사회 내 청소년조례 설치를 위한 노력, 정책예산의 차이를 최소화하기 위한 국고보조금의 차등보조율제도의 적용, 전달체계의 접근성 확보를 위한 노력이다. 본 연구는 기초자료로써 향후에도 청소년정책 인프라에 관한 세부적인 논의가 지속되어야 할 것이다. This study surveys the distribution of youth policy infrastructure and its regional differentiation. The local communities which are analyzed in this study comprise 228 cities, counties, and districts, and the policy infrastructure analyzed comprises the youth ordinances, the youth policy budgets, and the youth policy delivery systems within these local communities. Since these factors are data compiled by way of administrative division, the Geographical Information System(GIS) has been used to identify the geographical distribution. As a result of the analysis on the youth policy infrastructure distribution, the results confirm that all the ordinances, budgets, and delivery systems showed huge regional differentiation. The ordinances ranged between 1 and 12 and the budget for the youth policy took up 0.0% to 4.1% of the total budget, and there was a region’s youth policy budget taking up a maximum of 0.000% of the total budget. Lastly, the delivery system was divided into facilities and dedicated organization. The number of facilities showed a distribution between 1 and a maximum of 65, depending on regions, and the location quotient, which was analyzed to identify the region-specific concentration of facilities, ranged from between 0.2 and 17.4. Based on these results, this study suggests introducing a plan which can help to reduce the regional differentiation by bringing greater fairness and equality in the youth policy infrastructure. The major components of such a plan include greater efforts to enact youth ordinances within local communities, the application of differentiated national subsidy ratios to minimize the gaps in the youth policy budgets, and endeavoring to secure greater access to delivery systems. This study can provide basic data which can help to promote more detailed research and further discussions on youth infrastructure policies.

EU 난민정책 형성과정에서 집행위원회(European Commission)의 역할

정지윤(Ji Yun Jung),박성호(Sung Ho Park) 연세대학교 동서문제연구원 2017 동서연구 Vol.29 No.3

본 연구는 다층거버넌스 (multi-level governance, MLG)이론에 기반하여, 유럽연합(European Union, EU)의 난민정책 형성과정을 분석한다. 특히 EU 집행위원회(European Commission)가 정책결정에 미친 영향력에 초점을 두고, 이를 설명하는 초국적 인과메커니즘을 탐구한다. 핵심가설은 집행위원회가 EU회원국 및 이사회 기구에 정책결정의 영향력을 행사하는 데에, 해당 이슈영역의 권위적 국제기구인 UN난민기구(United Nations High Commissioner for Refugees, UNHCR)와의 초국적 정책연합이 중요 요인으로 작용했다는 것이다. 이러한 가설을 검증하기 위해 본 연구는 현 EU 난민정책의 근간이 형성된 2000년부터 2005년까지의 주요 정책의 도입과정을 살펴본다. This article applies multi-level governance theory to examine refugee policy making in the European Union (EU). The authors focus on the role of European Commission in the policy making process, identifying a transnational causal mechanism which explains the Commission s varying influence vis-a-vis member states and other EU institutions. The core argument is that the Commission s influence depended on whether or not it could build a transnational policy coalition with UNHCR(United Nations High Commissioner for Refugees), the most established international organization on refugee issues. The authors support their claim by examining major refugee policies, introduced during the 2000-2005 periods, which established the foundation of the EU refugee system.

조기위암의 점막하박리술 후 발생한 위주변 농양

정지윤 ( Ji Yun Jung ),김준성 ( Joon Sung Kim ),김병욱 ( Byung Wook Kim ),박성민 ( Sung Min Park ),김기준 ( Gi Jun Kim ),류승지 ( Seung Ji Ryu ),김영욱 ( Young Wook Kim ),지정선 ( Jeong Seon Ji ),최황 ( Hwang Choi ) 대한소화기학회 2016 대한소화기학회지 Vol.67 No.3

Endoscopic submucosal dissection (ESD) for early gastric cancer (EGC) is a widely accepted and well established procedure because of its curative potential and low invasiveness compared with surgery. Perforation is a potential major complication during ESD, and non-surgical treatments such as endoscopic closure with clips are sufficient in most cases. Here, we report a case of perigastric abscess that occurred as a complication of ESD for EGC. The patient improved with administration of antibiotics without surgical intervention. (Korean J Gastroenterol 2016;67:142-145)