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Pseudomonas sp. JH007에 의한 DL-2-Chloropropionic Acid로부터 D-Lactic Acid의 생산
정자헌,황인균,방원기 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.3
DL-2-chloropropionic acid로부터 D-lactic acid를 생산하기 위하여, 토양으로부터 DL-2-chloropropionic acid를 유일한 탄소원 및 에너지원으로 이용할 수 있는 균주 80여종을 분리하였으며, 분리균주들로부터 DL-2-chloropropionic acid로부터 D-lactic acid 생산성이 우수하며, L-lactic acid를 생산성이 우수하며, L-lactic acid를 생산하지 않은 균주 JH-007을 선별하여 Pseudomonas sp.로 동정하였다. DL-2-chloropropionic acid로부터 D-lactic acid를 생산하기 위한 최적 조건을 조사하기 위하여 3 g/l의 DL-2-chloropropionic acid가 포함된 LB 배지에서 배양하여 수확한 후 수확된 균체를 효소원으로 사용하였다. D-lactic acid 생산시 최적 반응액 조건은 125 mM sodium carbonate buffer에서 휴지균체 10 g/l와 3 g/l의 DL-2-chloropropionic acid를 사용할 때였으며, 최적 반응 pH는 10.0, 최적반응 온도는 30℃이었다. 최적조건하에서 반응액에 1 g/l의 DL-2-chloropropionic acid를 간헐적으로 첨가하여 5시간 반응시켰을 때 5.72 g/l의 D-lactic acid가 생산되었으며, 전환율은 98.4%였으며, 광학순도는 99.8%이었다. For the production of D-lactic acid from DL-2-chloropropionic acid, about 80 strains of bacteria capable of assimilating DL-2-chloropropionic acid as a sole carbon and energy source were isolated from the soil. JH-007 strain that showed the higest productivity of D-lactic acid and didn't produce L-lactic acid from DL-2-chloropropionic acid was selected from them and identified as Pseudomonas sp. The optimal conditions for the production of D-lactic acid from DL-2-chloropropionic acid were examined. The resting cells of JH-007 cultured in LB medium containing 3 g/l of DL-2-chloropropionic acid were used as an enzyme source. The reaction mixtures for the maximal production of D-lactic acid were consist of 10 g/l of resting cells and 3 g/l of DL-2-chloropropionic acid in 125 mM sodium carbonate buffer. The optimal pH for the reaction was 10.0 and the optimal temperature was 30℃. When 1 g/l of DL-2-chloropropionic acid was added intermittently to the reaction mixture under the above condition, 5.72 g/l of D-lactic acid was produced after incubation of 5 hrs. This amount of D-lactic acid corresponded to a 98.4% yields and the optical purity was 99.8%.
전신성 홍반성 루푸스의 진단 실태 및 지연 요인과 예후와의 연관성
정자헌 ( Ja Hun Jung ),심승철 ( Seung Cheol Shim ),장대국 ( Dae Kook Chang ),김태환 ( Tae Hwan Kim ),전재범 ( Jae Bum Jun ),정성수 ( Sung Soo Jung ),유대현 ( Dae Hyun Yoo ),김성윤 ( Seong Yoon Kim ),배상철 ( Sang Cheol Bae ) 대한류마티스학회 2001 대한류마티스학회지 Vol.8 No.3
Objective: This study is aimed to investigate clinical time course in the diagnosis of SLE, clinical features at disease onset, and their effects on organ damage and disease activity Methods: We evaluated 244 patients in the Korean Hanyang Lupus Cohort by medical record review and direct interview: the time at disease onset, clinical diagnosis, and diagnosis meeting ACR criteria for SLE (ACR diagnosis), clinical features at onset, SLICC/ACR damage index, SLAM-R, education, and income. The risk factors of delayed diagnosis and their effect on damage and activity were examined with uni and multivariate analyses. Results: Mean age and disease duration were 33.9 (±11.9) and 6.2 (±4.4) years. The mean time from onset to clinical diagnosis and ACR diagnosis were 2.4 (±3.6) and 2.7 (±3.3) years. The clinical features at disease onset were as follows; arthritis (46.3%), skin rash (13.7%), fever (7.4%), hematologic disorder (7.4%), nephritis (5.7%), Raynaud phenomenon (3.7%), neuropsychiatric syndrome (2.4%). Diagnosis was delayed when oral ulcer, photosensitivity, and arthritis were presented as 1st clinical features compared to when nephritis, malar rash, and fever as 1st clinical features. Delayed diagnosis (based on median time in clinical diagnosis) was not associated with damage, disease activity, education, and income. Conclusions: SLE was diagnosed late over 2 years after first symptoms onset and the time interval from clinical symptoms to ACR diagnosis is about 4 months; the delayed diagnosis and the prognosis in terms of damage and activity might depend on 1st clinical features.
정자헌(Ja Hun Jung),손동현(Dong Hyun Sohn),박준용(Joon Yong Park),오광택(Kwang Taek Oh),한동수(Dong Su Han),양석철(Suck Chul Yang),손주현(Ju Hyun Sohn),김순길(Soon Kil Kim),김호중(Ho Jung Kim) 대한내과학회 1996 대한내과학회지 Vol.51 No.4
N/A Objectives: Hyponatremia is the most common finding of electrolyte disorder in patients admitted to medical ward. It is suggested that the prognosis of patients with hyponatremia is worse than that with normal sodium concentration in morbidity and mortality. We performed the study to evaluate the incidence and cause of hyponatremia in inpatients admitted to medical ward via emergency room with prospective study. Methods: Hyponatremia was defined as a serum sodium concentration equal to or less than 134 mEq/L. A total 225 medical patients were prospectively studied for 45 days. Other chemistry and electrolytes of blood and urine were determined within 24 hrs following admission. Results: 1) Of medical inpatients through emergency room, hyponatremia was detected in high frequency (16%) and mild hyponatremia (125-134 mEq/L) was showed in 90% or more. 2) The most common cause of hyponatremia was decreased effective circulating blood volume group (76%), and its common underlying disease was liver disease. 3) Compared to other groups, the group with decreased effective circulatory volume showed significant decrease in spot urine sodium concentration, fractional excretion of uric acid and serum creatinine concentration. 4) There was no correlation between serum concentration of hyponatremia and other parameters associated with it. As it was corrected, no significant simultaneous changes on BUN and serum uric acid concentration was noted. Conclusion: Interpretation of this data depend on not decreased circulatory volume but also other compounding factor as production and excretion of which is related to underlying variable disease.