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석회보르도액 살포농도 및 시기가 3년생 인삼의 생육과 병방제에 미치는 효과
정원권,안덕종,최진국,장명환,권태룡 韓國藥用作物學會 2013 한국약용작물학회지 Vol.21 No.3
Lime-bordeaux mixture has been used to prevent diseases in the field of ginseng. The purpose of this study is to investigate the efficacy of lime-bordeaux depending on the concentration and treatment time on major diseases of ginseng such as Alternaria blight and anthracnose, and to evaluate the root growth of ginseng. Lime-bordeaux caused damage on leaf when it was sprayed on ginseng between April and early May. No difference was found in root growth by spraying lime-bordeaux mixture between ratio 4-4 and 8-8 ratio in concentration. Plot of 6-6 raito and 8-8 ratio appeared to be similar efficacy compared to that of practical chemical control. However, the plot of 4-4 ratio showed lower than that of chemical control.
석회보르도액 처리농도 및 시기가 4, 5년생 인삼의 생육과 병발생에 미치는 영향
정원권,안덕종,최진국,류태석,장명환,권태룡 한국약용작물학회 2014 한국약용작물학회지 Vol.22 No.6
Lime-bordeaux mixture (LBM) has been used instead of pesticides in ginseng field and orchard since the1960’s in Korea. In this experiment, LBM was made with different concentrations and sprayed in the field of ginseng foreco-friendly cultivation. Growth characteristics and disease such as alternaria blight, anthracnose, and gray mold wereinvestigated in 4-5 year old ginseng after spraying LBM. LBM caused a little damage on leaf when it was sprayed at the timeof leafing stage, late April and early May. Root weights of five-year-old ginseng were 43.1 ~ 51.5 g and 41.2 ~ 46.6 g in theplot of mid-April and mid-May treatments, respectively. These growth levels were further reduced as compared with that ofthe chemicals treatment plot. The rate of diseases in the plot of 6-6 and 8-8 ratio were 0.0 ~ 4.8% and 0.0 ~ 4.4%, respectively,which was similar with that in the plot of chemical control for alternaria blight and anthracnose. However, LBM hadlittle effect on controling gray mold. It showed lower control effect in the plot of 4-4 ratio than that of chemical control. Thisresult will be expected to be a useful guide that can be used in the field to the farmers of the ginseng.
인삼(Panax ginseng)에 발생한 Watermelon mosaic virus의 새로운 병원성 계통
정원권,이수헌,남문,이주희,박충열,김병훈,박은혜,이민아,김미경,최홍수,이준성,김정수,최진국,권태룡,이기운 한국식물병리학회 2013 식물병연구 Vol.19 No.4
A disease, supposedly caused by a virus, was observed from Insam (Panax ginseng) fields of Punggi in year2006. It has long believed to be a physiological disorder. However, the incidence of the disease has increasedevery year. When several samples were observed under electron microscope, filamentous virus-like particleswere observed. The nucleotide sequences of the virus were analyzed by RT-PCR with specific primer setsderived from the results of DNA chip. The results indicated that the disease was caused by Watermelon mosaicvirus (WMV). It revealed that the result of the biological assay by the virus was different from that of WMVpreviously found in other crops. Therefore, this is the first report that WMV causes the disease in P. ginsengand the virus is named to be WMV-Insam. 2006년 풍기지역 인삼(Panax ginseng)에서 바이러스병유사증상이 관찰되었으며, 매년 발병율이 증가하였다. 인삼에서 이러한 증상은 지금까지 생리장해의 일부로 다루어졌다. 그러나 전자현미경에서 사상형 바이러스가 관찰되었고, DNA 칩 및 염기 서열 분석으로 Watermelon mosaicvirus(WMV) 감염으로 인한 결과로 확인되었다. 인삼에발생한 WMV 분리주는 생물검정에서 기존에 알려진 WMV분리주들과는 새로운 병원성 계통으로 확인되어 WMV-Insam 계통으로 명명하였다. 본 논문은 인삼에 발생한 WMV에 관한 최초보고이다.
한지형 마늘 생장점 배양체의 세대별 바이러스 감염양상과 생육특성
정원권,이지은,이문중,도한우,연일권,박석희 한국국제농업개발학회 2020 韓國國際農業開發學會誌 Vol.32 No.1
1. 경북지역 한지형 마늘 주산지인 의성지역을 중심으로 OYDV, LYSV, GCLV, SLV 그리고 Allexivirus를 진단한 결과, 모든 마늘잎의 시료가 한 가지 이상의 바이러스에 복합감염되어 있었다.. 2. 생장점 배양한 마늘은 1세대부터 3세대까지 OYDV와 SLV는 전혀 검출되지 않았다. 생장점 배양 마늘의 1세대는 GCLV 3.8%, Allexivirus 3.1%의 바이러스 감염률을 나타내었으며, 생장점 배양 마늘 2세대는 LYSV 3.4%, GCLV 21.7% 그리고 Allexivirus 10.0%를 나타내었다. 생장점 배양 마늘 3 세대는 GCLV 17.5%, Allexivirus 7.5%의 바이러스 감염률을 나타내었다. 3. 의성지역의 바이러스에 감염된 일반마늘 1통의 평균 무게는 29.3 g 이었으나, 생장점 배양에 의하여 증식된 2세대 마늘 1통의 평균 무게는 57.6 g, 3세대 마늘 1통의 평균 무게는 66.2 g 이었다. All conventional garlics cultivated at farmer’s fields were infected with various plant viruses such as Onion yellow dwarf virus (OYDV), Leek yellow stripe virus (LYSV), Garlic common latent virus (GCLV) and Allexivirus. The infection rate of these viruses in northern-type garlics was from 14% to 100% in Uiseong and Yecheon area. For producing virus-free garlic, meristem was removed from garlic plants and cultivated in Murashige & Skoog (MS) medium. No OYDV and Shallot latent virus (SLV) were detected on leaf of garlic from first generation to 3rd generation. Virus infection rates of 2nd generation of garlic plants grown in net houses were 3.4%, 21.7% and 10.0% for LYSV, GCLV and Allexivirus, respectively, and those of 3rd generation were 17.5% and 7.5% for GCLV and Allexivirus, respectively. The average weight of a bulb infected with viruses in Uiseong was 29.3 g but that of bulbs from meristem culture in 2nd and 3rd generations were 57.6 g 66.2 g, respectively, showing more than two times increase in bulb weight.
Serratia plymuthica에 의한 인삼 세균뿌리썩음병 발생
정원권,김영수,최진국,김승한,장명환,권태룡,전용호 한국식물병리학회 2018 식물병연구 Vol.24 No.4
In August of 2011, a wilting disease of ginseng was observed at Bongwha, Gyeongbuk province, Korea. Affected plants initially show withering symptoms on leaves of ginseng. As the disease progresses, withering leaves spread downward, eventually encompassing the whole plant. Leaves lose vigor but remain pale green. Symptoms of roots were brown, and soft rots characterized by moist and watery decay of the whole ginseng root, which initiated as small brown, water-soaked lesions of hairy roots and enlarged to the entire roots. The causal organism isolated from the infected roots was identified as Serratia plymuthica based on its physiological and biochemical characteristics, by cellular fatty acid composition (GC-FAME), the utilization of carbon sources (BioLog System), and 16S rRNA sequence of the isolated bacterium were 99% homologous to those of Serratia plymuthica strains. Artificial inoculation of the bacterium produced the same brown or soft rot symptoms on the ginseng roots, from which the same bacterium was isolated. This is the first report of bacterial root rot caused by the Serratia plymuthica in ginseng in Korea. Serratia plymuthica has been used as antagonistic microorganism for biological control on several crop plants. But it was proved pathogen of ginseng at humid condition in this study.