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알코올 의존 환자의 적혈구막 지질과산화 정도와 적혈구 평균 용적 및 간 효소 지표와의 관련성
정성윤,최인근,서국희,강희정,Jeong, Seong Yun,Choi, Ihn-Geun,Suh, Guk-Hee,Kang, Hee Jung 대한생물정신의학회 1998 생물정신의학 Vol.5 No.2
Objectives : Alcohol-induced oxidative stress has been known to injure various tissues or organs. This stress is related with free radicals which are produced as the result of long-term alcohol consumption. Malonyldialdehyde(MDA) is produced by the interaction of free radicals and cell membrane lipids, and indicates the degree of lipid peroxidation indirectly. The purpose of this study was to investigate the relationship between red blood cell(RBC) membrane lipid peroxidation by free radicals, and associated hepatic injuries and hematologic changes. Methods : Thirty-three subjects diagnosed as alcohol dependence according to DSM-IV diagnostic criteria were evaluated within 72 hours after discontinuing alcohol drinking. Clinical characteristics were evaluated by CAGE questionnaire and Korean Michigan Alcoholism Screening Test(MAST). RBC membrane MDA level was measured as the marker of RBC membrane lipid peroxidation. Aspartate aminotransferase(AST), alanine aminotransferase(ALT) and gamma-glutamyltransferase(GGT) were used as the biochemical markers of liver damage due to alcohol ingestion. The alcohol-induced hematologic change was assessed by mean corpuscular volume(MCV). Results : The results were as follows. Clinical characteristics were not different between two groups having normal and abnormal levels of AST, ALT, GGT or MCV. The levels of MDA were not correlated with the clinical characteristics and serum levels of AST, ALT and GGT. However, there was a significant correlation between the levels of MDA and the value of MCV(p=0.017). Conclusions : These findings suggest that oxidative stress in alcohol dependence may not be reflected in liver enzyme markers such as AST, ALT and GGT, but may be reflected in MCV.
미생물 성장에 따른 Alexandrium catenella와 Chattonella marina에 대한 살조활성의 관계
정성윤 ( Seong-yun Jeong ) 대구가톨릭대학교 자연과학연구소 2019 자연과학연구논문집 Vol.17 No.1
In order to exploit the novel algicidal bacteria as a useful biological agent against harmful algal blooms (HABs), marine bacterial strain was isolated. The inhibitory effect for the growth of HAB species was examined. The isolated strain DC-31 exhibited significant algicidal activity against HAB species, Alexandrium catenella and Chattonella marina. The isolated algicidal bacterium was identified as Janibacter sp. The algicidal activity of the isolate was bacterial growth-dependent, since the strongest algicidal activity occurred in early stationary-phase cultures. A concentration of < 5% showed strong algicidal activity against A. catenella and C. marina. These results indicates that the algicidal activity of DC-31 is potentially useful in controlling HABs. In addition, it was demonstrated that the filtrate of DC-31 were very effective at killing or inhibiting of HAB species.
정성윤 ( Seong-yun Jeong ) 대구가톨릭대학교 자연과학연구소 2021 자연과학연구논문집 Vol.19 No.1
A bacterial strain CU-02 snowed algicidal activity against Alexandrium catenella was isolated from coastal water of Pohang in Korea. The strain was identified as Brachybacterium sp. by culture morphology and biochemical tests. The bacterial culture led to the lysis of algal cells, suggesting that the isolated strain produced a potent algal-lytic compound. Amongst changes in algicidal activity by different culture filtrates, the 10% concentration showed the biggest change in algicidal activity. Estimated algicidal activity was 89%. The swimming movements of A. catenella cells were inhibited and cell walls detached from the main cellular bodies because of treatment of the bacterial culture. With longer exposure time, A. catenella cells were disrupted and algal chloroplasts and cytoplasm subsequently lost their integrity and decomposed, resulting in the appearance of abundant broken thecae. The released algicides were heat-tolerant and stable in pH variations except pH 3.0 and 4.0. When 25 ㎍/㎖ of the crude algicides was applied to A. catenella culture, 89% of A. catenella cells were disrupted within 7 hours. The crude algicides showed significant algicidal activities against other harmful algal bloom (HAB) species. Our results suggest that the algicidal activity of Brachybacterium sp. CU―02 is potentially useful for controlling outbreaks of A. catenella
임상분리 Methicillin Resistant Staphylococcus aureus에 대한 항균활성 시너지 효과
정성윤 ( Seong-yun Jeong ) 대구가톨릭대학교 자연과학연구소 2017 자연과학연구논문집 Vol.15 No.1
최근 병원 감염의 주 원인균이며 기존 항생제에 다제내성을 보이는 MRSA(Methicillin Resistant Staphylococcus aureus)가 문제가 되고 있다. 다제내성균에 대한 치료제로 vancomycin이나 teicoplanin이 사용되고 있으나 그 사용이 아주 제한적이며, 최근에는 이들 항생제에 대한 내성균주가 많이 출현하여 새로운 항생제 개발이 절실히 요구되고 있다. 따라서 본 연구에서는 해양 미생물에서 분리한 항MRSA 활성을 나타내는 YJ-1 균주의 최적배양 조건을 확립하고, 이 균주가 생산하는 항균활성물질을 분리, 정제하여 신규 항생제제로서의 가능성을 제시하고자 하였다. 울진에서 해수를 sampling한 후 가장 강력한 anti-MRSA 활성을 나타내는 YJ-1 균주를 선택하여 분류, 동정하여 Pseudomonas sp. YJ-1으로명명하였다. 본 균주의 최적 성장 온도는 25℃였으며, 20℃와 30℃에서 성장에 큰 차이는 없었으며, 10℃와 4℃에서는 거의 성장하지 못 하였다. 최적 pH는 7.0으로 나타났으며, pH 6.0-9.0 사이에서 비교적 양호한 성장을 보였고, pH 4.0과 10.0에서는 거의 성장하지 못 하였다. 최적 NaCl 농도는 1%에서 가장 높게 나타났지만 NaCl 0% 농도에서와 큰 차이는 없었고, 3.0%까지 양호한 성장률을 보였으나 염농도가 높을수록 점점 성장률이 낮아지다가 염농도 7.0 이상에서는 거의 성장하지 못 하였다. 각종 유기용매 추출, C18 ODS open column, silica gel chromatography, reverse-phase HPLC 등을 이용하여 anti-MRSA 활성을 나타내는 순수 물질을 얻었으며, 이 중 MR1 물질은 임상분리 MRSA 균주인 DA 12와 DA 16 균주에 대해서도 비교적 우수한 활성을 나타내었다. MR1 물질을 농도별, 시간별로 DA 12와 DA 16 균주에 접종하여 항균활성을 비교한 결과, MR1은 vancomycin과 비슷하거나 오히려 높은 항균활성을 나타내어 MRSA에 대한 새로운 항생제 개발의 가능성을 제시 할 것으로 판단된다. 또한 MR1과 vancomycin과 함께 처리했을 때의 항MRSA의 시너지 효과를 조사한 결과, 임상분리 균주인 DA 12와 DA 16에 대해서 모두 우수한 시너지 효과를 나타내었다. 이상의 결과 향후 YJ-1으로부터 정제한 최종 물질의 화학적 특성과 구조를 규명하고, 작용기작을 밝힌다면 차세대 항생제로서의 개발 가능성이 높을 것으로 판단된다. It was isolated marine bacterium, isolate YJ-1 which produced a bactericidal antibiotics against MRSA. This isolate YJ-1 was identified to the genus Pseudomonas and was designated Pseudomonas sp. YJ-1. Pseudomonas sp. YJ-1 relatively grows well in the 25℃, pH 7.0, NaCl 1.0 %. For the purification of the bioactive compounds, YJ-1 was fermented in 37 L PPES-Ⅱ medium, and culture filtrates of YJ-1 was extracted by ethyl acetate, hexane, and 80% MeOH. The 80% MeOH fraction was separated by C18 ODS open column, silica gel chromatography and reverse phase HPLC to yield three anti-MRSA agent, MR1 compound. Compared with vancomycin and ampicillin, the MR1 has the potent activity against MRSA. In addition, the combination of MR1 compound with vancomycin produced a more rapid decrease in the clinical isolates, DA 12 and DA 16 MRSA cells than MR1 compound alone. Taken together, our results suggest that Pseudomonas sp. YJ-1 and its anti-MRSA compound could be employed as a natural antibacterial agent in MRSA infection.
BLE 비콘을 이용한 터널구간에서의 안전운행 정보시스템 개발에 관한 연구
정성윤(Seong-Yun Jeong) 한국산학기술학회 2015 한국산학기술학회 학술대회 Vol.2015 No.1
본 논문은 계속해서 증가하고 있는 도로터널에서의 교통사고를 예방하기 위해 터널구간을 통행하는 운전자에게 안전운행정보를 제공할 수 있도록 저전력 블루투스(Bluetooth Low Energy, BLE)방식의 비콘(beacon)을 이용한 안전운행정보시스템 개발을 구상하였다. BLE 비콘은 저전력, 초소형, 넓은 가용거리, No pairing, 1:N 서비스 등의 장점 때문에 차세대 근거리 데이터통신기술로 주목을 받고 있는 최신의 위치기반 서비스(LBS) 기술이다. 아울러 BLE 비콘을 이용한 안전운행정보시스템을 도입할 경우에 경제적 타당성을 분석하였다.