CTLL - 2 세포주에서 transforming growth factor - β가 CD8의 발현유도에 미치는 영향

박수영(Soo Young Park),장윤혜(Yoon Hae Chang),조영주(Young Joo Cho) 대한천식알레르기학회 2000 천식 및 알레르기 Vol.20 No.1

N/A Background: Transforming growth factor-β (TGF-β) has multiple regulatory effects on cells of the immune system, and it has been suggested that differentiation of lymphoid cells is influenced by low concentrations of this cytokine. Objectives : The aim of this study was to investigate the role of TGF-β in regulation of T cell growth and differentiation, and to compare this effect with that of other cell signals known to be important in T cell ontogeny. Methods .We used the CTLL-2 cell line in the presence of IL-2. Surface phenotype expression was analysed to see whether these cells could be switched to the other subtype of cells. Results : Treatment of CTLL-2 cells with TGF-β resulted in dose dependent growth inhibition and morphological changes. During routine passage, less than 5% of cells were CD8α positive, whereas 38% of cells expressed CD8α when treated with IL-2 plus TGF-β. However, TPA plus calcium ionophore, IFN-γ, or TNF-α caused no significant changes in the proportion of CD8 cells. Conclusion : Our results show that this experiment can be a useful model for investigating CD8 precursor potentials in populations of CD4-CD8- (double negative) cells, and such a model may offer a way to study the molecular regulation of CD8 gene expression.

3차 의료기관을 방문한 천식 환자에서 아토피 유병률의 변화

박흥우(Heung Woo Park),김상헌(Sang Heon Kim),김상훈(Sang Hoon Kim),장윤혜(Yoon Hae Chang),정희(Hee Chung),이병재(Byung Jae Lee),김윤근(Yoon Keun Kim),조상헌(Sang Heon Cho),민경업(Kyung Up Min),김유영(You Young Kim) 대한천식알레르기학회 2000 천식 및 알레르기 Vol.20 No.1

N/A Background: The prevalence of atopy and atopy-related disease has been known to increase during the past decade. Objective : This study was desinged to evaluate the changes of atopy prevalence among asthmatic patients and the causative allergens. Method : A total of 698 asthmatic patients visiting Seoul National University Hospital in 1983, 1990 and 1997 was recruited. Skin prick test with common inhalant allergens was carried out and interpreted as positive when the mean wheal size formed by allergen was larger than 3 mm. Atopy was defined when skin reaction to one or more allegens was positive. Result. The prevalence of atopy among asthmatic patients was 54% in 1983, 48.8% in 1990 and 58% in 1997, respectively. The prevalence of atopy decreased as the age of patients in- creased. Recently, old asthmatic patients ( >55 years) showed a higher tendency to visit the tertiary referral hospital. When the data were analyzed according to age, the prevalence of atopy significantly increased among asthmatic patients aged 15-35 years, in whom the prevalence was 60.4% in 1988 and 81.3% in 1997 (p< 0.05). The positive rate of skin test to Dermatophagoides pteronyssinus, cat fur, mugwort, ragweed, and grass pollen were significantly higher in 1997 than in 1983 (p < 0.05). Conclusion : The prevalence of atopy among asthmatic patients aged 15-85 years showed significant increase in 1997 compared to 1983. The changes of skin response to Dermatophagoides pteronyssinus, cat fur, mugwort, ragweed, and grass pollen contributed to the increase of atopy.

마우스 CD4 CD8 음성 세포 및 CD8 양성세포의 Interleukin 2에 의한 CD8 양성 세포와 자연살해 세포로의 전환 (The effect of interleukin 2 on the induction Of Nk 1.1 expression in CD8+ and CD4 - CD8 - T Cell)

조영주,장윤혜 (Young Joo Cho,Yoon Hae Chang) 대한천식알레르기학회 1998 천식 및 알레르기 Vol.18 No.2

Background: Murine IL-2-induced lymphokine-activated killers can be divided into two mutually exclusive subset:NK1.1'CD8 and NK1.1 CD8+. However, there is a strong evidence that NK cell may belong to T cell lineage. Recently novel lymphocyte subsets, present in the adult murine thymus, CD3+NK1.1'TCRap(TNK) cell is readily identifiable in fresh obtained murine adult CD4 CD8 thymocytes. Material and method: We sorted out CD4 and CD8 (double negative.' DN) cells and CD8+ cells from murine spleen and cultivated these cells with IL-2. And the surface B220, CD8, NK1. 1 and cytopasmic NK1.1 was analysed simultaneously to see whether these cells can be switched to the other subtype of cells. Result: Purified DN cells were switched to several subtype of cells'. CD8'B220+(LAK cells), NK1.1'B220+(LAK cells), CD8 B220, cytoplasmic NK1.1+B220 cells. Purified CD8 cells were switched to CD8+B220' LAK cells and cytoplasmic NK1.1+ CD8+ B220+ and cytoplasmic NK1.1' CD8 B220 cells. In addition, the CD8' cells originated from DN cells do not express the cytoplasmic NK1.1 in contrary to the sorted CD8 cells. Conclusion .' Our results indicated that these will be useful models to investigating CD8 precursor potentials in populations of CD4 CD8 (doble negative) cells and relationship of NK1.1 These results also supports the hypothesis that T cells and NK cells have same ontogeny and CD8 effector functions are potentially diverse and could be exploited by various conditions that switch off host protected cytolytic response. These model offer a way to study the molecular regulation of CD8 gene expression.

중증 기관지천식 환자의 말초단핵구에서 자연살해세포의 비율과 자연살해세포에서 interferon gamma 의 생산

천은미(Eun Mi Chun),김미선(Mi Sun Kim),장윤혜(Yoon Hae Chang),박성숙(Sung Sook Park),조영주(Young Joo Cho) 대한천식알레르기학회 2000 천식 및 알레르기 Vol.20 No.3

N/A Background: and objective: The natural killer (NK) cells which play an important role in defense immune system are supposed to be involved in the pathogenesis of bronchial asthma. The goal of this study is to analyze the role of NK cells in the pathogenesis of bronchial asthma by examining the proportion of NK cells in peripheral blood mononucler cells (PBMCs) and production of interferon gamma (IFN-γ) in NK cells between normal group and asthmatic group. Methods: Ten patients with moderate to severe persistent asthma sensitive to house dust mite were enrolled as asthmatic group. PBMCs were activated by 12-0-tetracanoylphorbol-13- acetate (TPA) and calcium inonophore for 18 hours. Surface CD3 and CD56 antigens with intracytoplasmic IFN-γ staining were performed simultaneously and the results were analyzed by three color fiow cytometer. Results : The percentage of CD56+ positive NK cells in PBMCs from asthma group was lower compared to control group (15.4±3.9% vs 19.8±4.5%). However, There was no signficant difference of IFN-γ production in CD56+ NK cells between two groups (30.3±3.9% vs 25.9±5.8%. P>0.05). IFN-γ producing CD3+ T cells were significantly higher in asthma group compared with normal control group (36.3±1.8% vs 28.4±5.7%, p<0.05). The ratio of TNK cells expressing both CD56 and CD3 was not different between asthma group and control group (4.7±1.4 % vs 5.9±1.8%, p<0.05). Conclusion : The results suggest that aggravation of asthma symptoms in severe asthma may be caused partly by decrease in NK cells. The increased production of IFN-γ in asthma patients suggest that IFN-γ may function as inflammatory cytokine.

만성기침 환자에서 유도객담내 호산구와 기관지과민성 및 천식 치료 반등도와의 관계

이병재(Byung Jae Lee),정재원(Jae Won Chung),장윤석(Yoon Suk Jang),장윤혜(Yoon Hae Chang),김상훈(Sang Hoon Kim),정희(Hee Chung),김윤근(Yoon Keun Kim),조상헌(Sang Heon Cho),민경업(Kyung Up Min),김유영(You Young Kim) 대한천식알레르기학회 2000 천식 및 알레르기 Vol.20 No.6

N/A Background: The value of the induced sputum examination in chronic cough has not been determined. We performed this study to investigate the relationship between eosinophil percentage on induced sputum and bronchial responsiveness to methacholine or capsaicin, and responses to anti-asthmatic treatment in chronic cough patients. Subjects and Methods Forty-seven patients with chronic cough persisting for more than 1 month without current wheezing or dyspnea were studied. According to the eosinophil percentage on induced sputum, the subjects were divided into two groups group A (sputum eosinophil ≥ 3%) and group B (sputum eosinophil < 3%). Methacholine bronchial provocation test (MBPT) and capsaicin challenge, and responses to anti-asthmatic treatment were compared between the two groups. Results : Group A consisted of 26 subjects and group B consisted of 21 subjects. There were no differences in sex, clinical characteristics of cough, atopy prevalence, and peripheral eosinophil counts except serum IgE level between the two groups. MBPT positivity was much higher in group A than group B (46.2% vs 0%, p<0.001), but there was no difference in capsaicin test positivity(44.0% vs 50.0%). Group A showed much higher response rates to anti-asthmatic treatments than in group B (73.1/o vs 19.09o, p(0,001). Conclusion : Eosinophilic airway inflammation in chronic cough was related to methacholine bronchial hyperresponsiveness, but not to capsaicin cough threshold. Induced sputum eosinophil percentage was a good indicator in predicting the response to anti-asthmatic treatment in most chronic cough patients. (J Asthma Allergy Clin Immunol 20: 895-905, 2000)

기관지천식 환자들에서 유병기간에 따른 기도의 구조변화

조영주,신미승,장윤혜,장중현,박성숙,최혜영 대한알레르기학회 1998 천식 및 알레르기 Vol.18 No.4

Backgrount: Bronchial asthma is classically defined as a reversible obstruction and hypsrresponsiveness of the airway attributed to an inflammatory process. However, some individuals with asthma show an irreversible component of airflow obstruction. It may be associated with structural changes in the airway resulting from severe or long standing airway inflammation and remodelling. Objective: The study was undertaken to compare the clinical characteristics of patient and airway remodelling as shown in bronchial wall thickness in HRCT according to the duration of asthma. Material and methods: A retrospective clinical study was done on 119 patients with bronchial asthma, who had been admitted to Ewha Womans University Mokdong Hospital. Patients were divided to three groups according to disease duration and, clinical characteristics, pulmonary function test and HRCT were done. Results: Basal FEV, and FVC was significantly lower in patient with longer duration. (p $lt;0. 05) However pulmonary function was improved regardless of disease duration after 2 weeks steroid and bronchodilator therapy, and there was no significant difference in level changes according to the disease duration. The inner diameter of the bronchi and thickness of the bronchial wall at segmental and subsegmental bronchi increased significantly in patient with longer duration of asthma(p$lt;0.05). Conclusion: These findings showed that airway remodelling was more extensive in patients with longer duration of disease resulting in decreased pulmonary function. These facts suggested that early anti-inflammatory therapy would be helpful for prevention of airway remodelling.

에폭시수지가 함유된 근관충전제 (root canal sealer) 에 의한 접촉 두드러기

김상훈,김유영,김윤근,정희,이병재,민경업,조상헌,장윤혜 대한알레르기학회 2000 천식 및 알레르기 Vol.20 No.2

Epoxy resin based on Diglycidyl-ether-Bisphenol-A has been commonly used in dental fillings and dentures. Epoxy resin is a well known low molecular weight chemical sensitizer, but contact urticaria from epoxy resin has been infrequently reported despite it's wide use in dental practice. We report a case of a 48-year-old housewife who presented a recurrent episode of perioral erythema and angioedema associated with dental work. These symptoms, which occurred within 2 hours of having dental fillings, were aggravated with repeated dental procedures for a period of several days. Her symptoms improved after removal of dental fillings. Her contact urticaria to epoxy resin was reproduced by patch test with AH26 root canal sealer containing Bisphenol-A type epoxy resin. Delayed type reaction to epoxy resin was also noted. Her symptoms did not reoccur after use of another type of root canal sealer.

Phenobarbital에 의한 anticonvulsant hypersensitivity syndrome 1례

김상훈,김유영,김윤근,정희,이병재,민경업,조상헌,장윤혜 대한알레르기학회 2000 천식 및 알레르기 Vol.20 No.1

Anticonvulsant hypersensitivity syndrome (AHS) is a potentially fatai drug reaction with cutaneous and systemic reaction to the arene oxide-producing anticonvulsants such as phenytoin, carbamazepine, and phenobarbital. In most cases, AHS characterized by fever, rash, lymphadenopathy and multiorgan-system abnormalities. Fatal outcomes are most often associated with liver failure. We experienced a 35-years-old man presenting typical characteristics of phenobarbital induced AHS. He presented fever, rash, lymphadenopathy, hepatitis, lung involvement and hematologic abnormalities. After use of systemic steroid, symptoms, signs and laboratory abnormalities were gradually disappeared. We report a case of AHS diagnosed by characteristic clinical and laboratory findings with a brief review of the literature.

관상동맥혈관조형술과 심내막조직검사로 증명한 심장과 관성동맥을 침범한 Churg - Strauss 증후군

김상훈,김상헌,김유영,김윤근,박흥우,정희,이병재,민경업,조상헌,장윤혜,연태진 대한알레르기학회 2000 천식 및 알레르기 Vol.20 No.2

Churg-Strauss syndrome (CSS) is a rare systemic disease characterized by vasculitis and peripheral eosinophilia in patients with an atopic constitution. Cardiac involvement is an important cause of morbidity and mortality in patients with CSS but coronary artery involvement is very rarely documented. We report a case of a 27-years-old female with CSS who had coronary vasculitis. Coronary angiography and endomyocardial biopsy demonstrated coronary vasculitis and cardiac involvement.