http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
장대식(Dae Sik Chang),김민석(Min Suk Kim),오병훈(Byeong Hoon Oh),정승호(Seung Ho Jeong) 대한전기학회 2010 대한전기학회 학술대회 논문집 Vol.2010 No.7
온도 측정을 위해서 다양한 종류의 온도 센서가 사용되어야 한다. 또한 Thermocouple과 같은 센서는 mV단위의 출력에 비선형 특성을 가지고 있기 때문에 일반적으로 signal conditioning 과정을 수행하고 그 결과를 범용 A/D 컨버터의 입력에 연결하여 데이터 계측 및 저장을 수행한다. 본 연구는 실험 장치에 설치되어 있는 다양한 온도 센서를 EPICS(Experimental Physics and Industrial Control System)를 사용하여 동일한 방법으로 모니터링 하기 위해 개발한 방법에 대한 것이다.
KIOM-79에 의한 p65 단백질의 핵내 이동 및 iNOS 발현 억제
김진숙(Jin-Sook Kim),장대식(Dae-Sik Jang),전영진(Young-Jin Jeon),유호진(Ho-Jin You),박경한(Kyeong Han Park),김 명(Jin Ming),문형윤(Hyung-Yoon Moon),윤상필(Sang-Pil Yoon),장인엽(In-Youb Chang) 대한해부학회 2006 Anatomy & Cell Biology Vol.39 No.2
후박 (Magnolia officinalis), 갈근 (Pueraria lobata), 감초 (Glycyrrhiza uralensis) 및 대극(Euphorbia pekinensis)은 염증성질병을 포함한 다양한 질병을 치료하기 위해 사용되었다. 본 실험에서는 각각의 천연물의 복합추출물인 KIOM-79가 큰포식세포에서 inducible NO synthase (iNOS) 유전자의 발현을 억제시키는 기전을 밝혀내기 위하여 면역형광염색, RT-PCR, electrophoretic mobility shift assay (EMSA) 등을 실시하였다. 면역형광염색 소견을 보면 RAW 264.7 세포에 lipopolysaccharide (LPS)에 의해 유도된 iNOS 단백질의 발현이 KIOM-79에 의해 억제되는 것을 알 수 있다. Western blot과 RT-PCR 분석에 의하면 KIOM-79가 LPS에 의한 iNOS의 발현을 억제함을 확인 할 수 있다. LPS와 KIOM-79를 처리한 RAW 264.7 세포에서 nuclear extract를 추출하여 EMSA로 분석한 결과, LPS에 의해 유도된 NF-κB/Rel의 DNA 결합이 KIOM-79에 의해 억제됨을 확인하였다. 면역형광염색 소견을 보면 NF-κB/Rel의 구성단백질 중의 하나인 p65단백질은 세포질에서 발현되고 있으며, LPS를 처리하면 p65가 핵으로 이동함을 알 수 있다. 이때 KIOM-79를 처리하면 LPS에 의한 p65의 핵 내 이동이 억제됨을 확인할 수 있다. 결론적으로, KIOM-79는 큰포식세포에 작용해 iNOS 유전자의 발현을 억제하며, 이러한 큰포식세포의 활성억제는 p65 단백질의 핵 내 이동 억제를 통해서 유도되는 것으로 사료된다. We demonstrate that KIOM-79, combined extracts isolated from Magnolia officinalis, Pueraria lobata, Glycyrrhiza uralensis, and Euphorbia pekinensis, inhibits LPS-induced expression of iNOS gene in RAW 264.7 cells. Treatment of RAW 264.7 cells with KIOM-79 inhibited LPS-stimulated nitric oxide production in a doserelated manner. Immunohisto-chemical staining of iNOS and RT-PCR analysis showed that the decrease of NO was due to the inhibition of iNOS gene expression. Immunostaining of p65 and EMSA showed that KIOM-79 inhibited NF-κ/Rel nuclear translocation and DNA binding, respectively. Collectively, this series of experiments indicates that KIOM inhibits iNOS gene expression by blocking NF-κ/Rel. Due to the critical role that NO release plays in mediating inflammatory responses, the inhibitory effects of KIOM-79 on iNOS suggest that KIOM-79 may represent a useful anti-inflammatory agent.
정진환,신평균,류진창,장대식,조성환 ( Jin Hwan Chung,Pyung Gyun Shin,Jin Chang Ryu,Dae Sik Jang,Sung Hwan Cho ) 한국응용생명화학회 1997 Applied Biological Chemistry (Appl Biol Chem) Vol.40 No.2
Platycodon root contains abundant pharmaceutical substances and is widely used as a food and a medicinal herb. The three-year-old and the 24-year-old Platycodon roots cultivated in a local farm were analyzed and compared with their pharmaceutical substances to identify the major components. Reverse column HPLC analysis of ethanol extracts from the 24-year-old roots showed some distintive peaks after the retention time of 50 min which were absent in the extracts from the three-year-old roots. This indicates that several pharmaceutical substances are present in the older roots. There were no differences in sterol composition and saponin content between the 24-year and three-year-old roots. The 15-year-old and the 23-year-old Platycodon roots, the 6-year-old Ginseng root, and the three-year-old Condonopsis root all did not show significant anticomplement activity.
정진환,신평균,류진창,장대식,조성환 ( Jin Hwan Chung Pyung Gyun Shin,Jin Chang Ryu,Dae Sik Jang,Sung Hwan Cho ) 한국응용생명화학회 1997 Applied Biological Chemistry (Appl Biol Chem) Vol.40 No.2
Platycodon root contains abundant pharmaceutical substances and is widely used as a food and a medicinal herb. To identify the major components of the three-year-old and the 24-year-old platycodon roots cultivated in a local farm were analyzed and compared with their chemical compositions prior to their pharmaceutical substances, which were used as the fundamental data. There were no differences in chemical composition, inorganic compositions and fatty acid compositions between the 24-year-old and three-year-old roots. Total amino acid contents were 375.8 ㎎% and 891.6 ㎎% in the 24-year-old and three-year-old platycodcon roots, respectively. Sixteen species of amino acid were identified by amino acid autolyzer except cysteine both in the 24-year-old and three-year-old roots. Linoleic acid, which was the highest fatty acid contained both in the 24-year-old and three-year-old roots, was estimated to be 48.7% and 39.1% of total fatty acids, respectively.
쑥갓의 꽃에서 분리한 sesquiterpene lactones의 항균활성
하태정,한효심,장기창,장대식,조동령,양민석,이경동,Ha, Tae-Jung,Han, Hyo-Shim,Jang, Ki-Chang,Jang, Dae-Sik,Cho, Dong-Young,Yang, Min-Suk,Lee, Kyung-Dong 한국응용생명화학회 2003 한국농화학회지 Vol.46 No.3
Thirteen sesquiterpene lactones isolated from the flower of Chrysanthemum coronarium L., which has been widely cultivated in Korea as a vegetable for a long time, were investigated their antimicrobial activities against eight bacteria and five phytopathogenic fungi. The antimicrobial activities of dihydrochrysanolide (6) and 1-epi-dihydrochrysanolide (7) showed strong activities against all the bacteria such as Bacillus subtilis, Bacillus cereus, Staphylococcus aureus and Vibrio parahaemolyticus. Especially, Staphylococcus aureus was investigated that have very strong antibacterial activity to $1.56\;{\mu}g{\cdot}disc^{-1}$, respectively. Also, most of sesquiterpene lactones, which have ${\alpha}-methylene-{\gamma}-butyrolactone$ group, were exhibited strong activity to Gram(+) bacteria than Gram(-) bacteria. In the antifungal test, Rhizoctonia solani and Phytophthora capsici known as phytopathogenic fungi have exhibited all extensive activity about compounds that have ${\alpha}-methylene-{\gamma}-butyrolactone$ group. 쑥갓(Chrysanthemum coronapium L.)의 꽃에서 분리한 13개의 sesequiterpene lactone들에 대한 생리활성 실험의 일환으로, 세균 8종과 곰팡이 5종을 이용하여 항미생물 활성을 검토하였다. 그 결과, 항세균 실험에서 Gram 양성균인 Bacillus subtilis, Bacillus cereus, Staphylococcus aureus 균들과 Gram 음성균인 Vibrio parahaemolyticus균이 쑥갓에서 분리된 13종의 화합물들에 대하여 광범위한 활성을 나타내었으며, 이들 중에 dihydrochrysanolide 유도체가 Staphylococcus aureus균에서 $3.1\;{\mu}g{\cdot}disc^{-1}$이하의 강한 항세균 활성을 보였다. 항진균 실험에서는 ${\alpha}-methylene-{\gamma}-butyrolactone$ group을 가진 화합물들이 Rhizoctonia solani와 Phytophthora capsici에 대해 강한 항진균 활성이 조사되었다.