산양유 및 산양유 발효유가 웅성 설치류의 생식기능과 지구력에 미치는 영향에 관한 연구

임경순,강재구,최기명,배창준,조우제,Im, Kyung-Soon,Kang, Jae-Ku,Choi, Ki-Myung,Pae, Chang-Joon,Joh, Woo-Jea 대한생식의학회 2000 Clinical and Experimental Reproductive Medicine Vol.27 No.4

Objective: The purpose of this study was to evaluate effects of goat milk and fermented goat milk on reproductive function and stamina of male rodent. Methods: Experiment I: Male ICR mouse was divided into four groups. Group 1 none-treated control; Group 2 received saline; Group 3 received cow milk 10 ml/kg per day for 15 days; Group 4 received goat milk 10 ml/kg per day for 15 days. The cauda epididymal sperm motility and testicular sperm production were investigated. Experiment II: Male SD rat was divided into three groups. Group 1 received saline; Group 2 received goat milk 10 ml/kg per day for 28 days; Group 3 received fermented goat milk 10 ml/kg per day for 28 days. The cauda epididymal sperm motility and testicular sperm production were also investigated. The concentration of testosterone in serum at 1 and 3 weeks after treatment was determined using Immulite 2000 kit. Testes, epididymis, prostate, and seminal vesicle were weighed. Experiment III: Male ICR mouse was divided into four groups. Group 1 none-treated control; Group 2 received saline; Group 3 received goat milk 10 ml/kg per day for 4 weeks; Group 4 received fermented goat milk 10 ml/kg per day for 4 weeks. After treatment, the mouse was forced to swim to test for stamina. Results: In Experiment I, the cauda epididymal sperm motility after in vitro culture for 1 or 3 h was significantly (p<0.05) higher in cow milk and goat milk than in the control and saline. There was no significant difference in the cauda epidymal sperm motility between cow and goat milk. The testicular spermatid number was significantly (p<0.01) higher in goat milk (222.8${\times}10^6$) than in the control (108.6), saline (98.2), and cow milk (118.2). In Experiment II, the cauda epididymal sperm motility after in vitro culture for 1 h was significantly (p<0.05) higher in fermented goat milk than in saline and goat milk. There was no significant difference in the cauda epidymal sperm motility between saline and goat milk but goat milk showed slightly higher sperm motility than saline. After in vitro culture for 3 h, the cauda epididymal sperm motility was significantly (p<0.01) higher in fermented goat milk and goat milk than in saline. The testicular spermatid number was significantly (p<0.05) higher in goat milk than in saline, and significantly (p<0.01) higher in fermented goat milk than in saline. And the serum testosterone levels of rats administered with goat milk or fermented goat milk were increased but were no significant difference among three groups. Also the prostate weight was significantly (p<0.05) increased in the goat and fermented goat milk. In Experiment III, the swimming time in the goat milk and fermented goat milk groups was significantly (p<0.01) longer than in the control and saline. There was no significant difference in the swimming time between goat and fermented goat milk but the fermented goat milk showed slightly longer swimming time than the goat milk. Conclusion: The cauda epididymal sperm motility, the testicular spermatid number and stamina were improved when the mice and rats were drunk with goat milk or fermented goat milk.

돼지 동결정액의 제조와 이용

임경순 ( Kyung Soon Im ) 한국축산학회 1983 한국축산학회지 Vol.25 No.7

우주적 실재에 관한 인식론적 성찰

김혜숙(Hei-Sook Kim),안창림(Chang-Rim Ahn),김성구(Seung-Ku Kim),임경순(Kyung-Soon Im) 한국철학회 2001 哲學 Vol.67 No.-

양자론과 관련한 인식론적 문제들은 지금까지 많이 논의되어왔다. 그러나 현대 우주론과 관련한 인식론적 문제는 거의 논의된 바가 없다. 우주론(cosmology)은 우주 자체에 관한 인간 경험의 한계 때문에 1960년대 이전까지는 사변적 요소를 많이 담고 있었으며, 과연 그것이 과학의 분야일 수 있는지에 관한 논란이 많았다. 그러나 이후 몇 십 년 간 우주론 분야는 눈부시게 성장하였고 고도로 이론화되었다. 본 논문은 우주의 시작과 끝에 관한 이론으로서의 우주론의 내용을 철학적 문제의식의 관점에서 살펴보고 그 안에서 제기되는 인식론적 물음들을 다룬다. 이 물음은 과학적 이론의 정당화와 진리, 검증 불가능한 과학이 론의 정당화 문제, 우주의 객관적 실재에 관한 이론으로서의 우주론 내에서 등장하는 인간원리의 문제들과 관련되어있다. 본 논문은 과학적 이론의 궁극적 정당화는 객관적 실재와의 대응이 아니라 (그 이론이 그 객관적 실재 자체의 기원과 생성에 관한 이론인 경우에는 더욱 그러하다.) 이론의 설명력과 이론 내에서의 수학적ㆍ논리적 정합성, 그리고 인간원리에 의존하는 일종의 선험논변에 의해 이루어짐을 초끈 이론의 경우를 통해서 보이고자 한다.

소의 受精卵 移植에 관한 硏究 : II. 性成熟 過程의 처녀우의 多排卵 誘起와 受胎에 관한 硏究

任京淳,李用斌,鄭丘敏,金鍾國 서울大學校 農科大學 1986 서울대농학연구지 Vol.11 No.1

This experiment was carried out to investigate the effects of superovulation on the ovarian response and embryo production of young and matured heifers. The possibility of mon-surgical recovery from the young heifers and transfer of recovered embryos to the mature recipients was also investigated. A total of 8 heifers(5 heads of Holstein, 2 Heads of Korean Native Cattle(KNC)and one head of Angus×Hereford crossbreed)were devided into 2 groups by their age. Young heifers(Group I)were 7∼10 months old and mature heifers(Group II)were 12∼18 months old. Group I and II were superovulated with muscular injection of 1,000 or 2,000 IU Pregnant Mare's Serum Gonadotrophin(PMSG)on day 10∼12 of estrus cycle followed by muscular injecion of 25mg Prostaglandin F ₂α(PGF₂α) 48 hours later. Heifers were artificially inseminated or copulated 2∼3 times with the interval of 12 hours while they were in heat. The embryos were collected non-surgically on day 6 of estrus cycle. The results obtained from this study were summarized as follows: 1. The average number of corpus lutea(CL) by the superovulation treatment was much greater in Group 11(9.2)than in Group 1(3.2) 2. The average number of unruptured follicles by the superovulation treatment was much greater in Group II(6.8)than Group I(1.0) 3. The interval to onset of estrus after PGF₂αinjection was longer in Group I(44.2 hours)than in Group II(31.8 hours).

토끼의 수정란의 동결 보존 및 이식에 관한 연구 Ⅲ. 초자화동결 및 융해가 토끼 상실배의 생존성에 미치는 영향

이현정,최화식,이창규,임경순 서울大學校農科大學 農業開發硏究所 1991 서울대농학연구지 Vol.16 No.1

본 실험은 토끼 상실배의 초자화동결에 있어서 동결액에 수정란의 노출, 동결액에서 평형시간, 스트로우내 수정란의 분주방법, 동결 후 동결액의 초자화 상태 및 동해방지제의 농도가 동결 융해후 수정란의 발생능에 미치는 영향을 구명하기 위하여 실시하였으며 얻어진 결과는 다음과 같다. 1. 토끼 상실배를 실온에서 10% glycerol + 20% 1,2-propanediol에 10분간 노출후 25% glycerol + 25% 1,2-propanediol 또는 30% glycerol + 30% 1,2-propanediol에 30초∼1분간 노출하고 배양했을 때 팽윤배반포로 발달한 수정란의 비율은 각각 86.6와 75%로 두동해방지제를 25%씩 첨가한 처리가 30%씩 첨가한 처리보다 현저히 높았다. 2. 토끼 상실배를 10% glycerol + 20% 1,2-propanediol에서 5 또는 10분간 평형후 동결 융해하여 배양했을 때 팽윤배반포로 발달한 수정란의 비율은 각각 75.0과 86.1%로, 5분보다 10분 평형시켰을 때가 수정란의 발생비율이 현저히 높았다. 3. 동결 융해 후 배양했을 때 팽윤배반포로 발생한 수정란의 비율은 스트로우내 초자화동결액 배열이 이중일경우가 86.1%로, 단순배열의 31.8%보다 현저히 높았다. 4. 동결 융해 후 배양했을 때 팽윤배반포로 발생한 수정란의 비율은 배를 함유한 세포외 동결액 부분이 초자화한 경우가 86.1%로, 비초자화한 경우의 28.0%에 비해 현저히 높았다. 5. 동결 융해 후 배양했을 때 팽윤배반포로 발생한 수정란의 비율은 세포외 동결액의 동해방지제의 농도 25% glycerol + 25% 1,2-propanediol 처리가 86.1%로 30% glycerol + 30% 1,2-propanediol 40.%, 20% glycerol + 20% 1,2-propanediol처리 0.0%보다 현저히 높았다. These experiments were carried Out to investigate the effects of exposure of embryos to vitrification solution, equilibration time in intracellular vitrification solution, the system of arrangement of vitrification solution to straw, the vitrified state of extracellular vitrification solution after freezing and the concentration of cryoprotectants in extracellular vitrification solution on the developmental ability of frozen-thawed rabbit morula to expanded blastocyst. The results are as follow. 1. When the rabbit morula were exposed to 25% glycerol +25% 1,2-propanediol or 30% glycerol + 30% 1,2-propanediol for less than 1 min after exposure to 10% glycerol + 20% 1,2-propanediol for 10 min and cultured, the ratio of embryos developed to expanded blastocyst was 86.6 and 75.0%, respectively. The 25% glycerol + 25% 1,2-propanediol showed higher developmental ability than 30% glycerol + 30% 1.2-propanediol. 2. When the rabbit morula were equilibrated at 10% glycerol + 20% 1,2-propanediol for 5 or 10min, the ratio of embryos developed to expanded blastocyst was 75.0% for 5 min and 86.1% for 10min. The 10 min equilibration time showed higher developmental potency than 5 min. 3. The ratio of embryos developed to expanded blastocyst after freezing and thawing was 86.1% in the Straw of double column and 31.8% in the straw of single column. The straw of double column showed significantly higher percentage of development than the straw of single column. 4. The ratio of embryos developed to expanded blastocyst after freezing and thawing followed by incubation was 86.1% when extracellular vitrification solution in straw was transparency and 28.0% when extracellular vitrification solution was opaque. The vitrified state of extracellular vitrification solution affected significantly the developmental potency of embryos after freezing and thawing. 5. When the embryos are vitrified at 20% glycerol + 20% 1,2-propanediol. 25% glycerol + 25% 1,2-propanediol and 30 glycerol + 30% 1,2-propanediol, the ratio of embryos developed to expanded blastocyst after freezing and thawing was 0.0, 40.0, 86.1% respectively. The highest developmental potency was obtained when the morula were vitrified at 25% glycerol + 25% 1 .2-propanediol vitrification solution.

돼지의 정액 희석액 개발에 관한 연구

임경순 서울대학교 농업개발연구소 1999 농업생명과학연구 Vol.3 No.-

This study was carried out to evaluate the effect of pH and storage temperature on the viability of boar sperm diluted with extender KpA for swine arificial insemination. The results obtained in this experiment were summarized as follows: 1. The viability of boar sperm diluted with KpA was higher than that with BTS during storage and especially more than 60% of sperm viability in KpA was maintained through 6 days. The pH values of all extenders were kept during storage of semens following dilution. 2. The sperm diluted with acidic (pH 6.3~6.8) or alkalic (pH 7.8~8.0) KpA and stored at 4℃ or 37℃ were more sensitive in viability than that with neural pH (6.8~7.3) and at 17℃ storage. But pH values of all conditions were not increased rapidly. Especially acidic and alkalic diluents were more stable in pH after dilution. Conclusively, extender pH and storage temperature were the important factors for sperm viability. The KpA setting up around neutral pH was the optimal boar semen extender for maintaining liquid semen at 17℃

소의 受精卵移植에 관한 硏究

任京淳,李用斌,鄭丘敏 서울大學校農科大學 1984 서울대농학연구지 Vol.9 No.1

These experiments were conducted to investigate ovarian response and estrus condition following 5 times repeated superovulations and to compare the recovery rate and the cleavage stage of embryos in superovulation and single ovulation in Korean Native Cattle(KNC). Four parous cows were superovulated with the musclar injection of 25mg PGF₂α 40-48hrs after 2,000IU PMSG injection on 9-12days of estrus cycle. The superovulations were induced repeatedly at 5 times with the intervals of 60±15, 90±10, 145±10 and 155±10 days, respectively, and the superovulated ova were recovered on 6-10days following estrus by non-surgical method. Ova were also recovered from other five single ovulated cows on 2-15 days following estrus by non-surgical method. Some embryos were transferred to KNC and Holstein recipients. The results obtained in this study are summarized as follows: 1. Average number of ova ovulated from A,B,C and D donors superovulated at five times was 7.4, 14.8, 3.8, and 8.4 and there was remarkable difference among donors. 2. Average number of ovulated ova in 1st, 2nd, 3rd, 4th and 5th repeated superovulations with 60±15, 90±10, 145±10 and 155±10 days interval between superovulations was 6.0, 10.3, 7,3, 10.8 and 10.3 The number of ovulated ova in 1st and 3rd was lower but that in 2nd, 4th and 5th was higher and there was no difference among 2nd, 4th and 5th superovulations. 3. The rate of small corpus lutea(<5mm) in 1st, 2nd, 3rd, 4th and 5th repeated superovulations with 55±10, 80±5, 90±5 and 150±15 days interval between superovulations was 12, 41, 35, 29 and 15%, respectively. The rate was decreased according to increase of interval. 4. The cows was comming into standing estrus after average of 30hrs following PGF₂α injection and 73.7% of the cows was comming into heat between 1-2days following PGF₂α. 5. The rate of normal estrus cycle (18-25days) between 1st, 2nd, 3rd, 4th and 5th repeated superovulations with intervals of 60±15, 90±10, 145±10 and 155±10days was 60.0, 40.8, 58.3 and 62.5%, respectively. The rate was increased according to increase of the intervals and the rate between 4th and 5th superovulations reached to the same level as that between 1st and 2nd superovulations. 6. The repeated superovulation showed lower average recovery rate of ova (33.4%) than that of the single ovulation. 85.7% The recovery rate of ova in 5th superovulations was 50.0, 13.6, 14.3, 37.2 and 48.4% and the rate of 2nd and 3rd superovulations with short intervals was lower than that of 4th and 5th superovulations with longer interval. 7. Of 48 ova recovered from the superovulated cows on 6-10 days after estrus, the rate of normal and abnormal ova(nonfertilized, degenerated and broken) was 60.4 and 39.6%, respectively. The cleavage stage of embryos was early-middle morula on 6½-7 days, middle-tight morular on 7½-8 days and tight morular-hatched blastocyst on 8½-10days. 8. Of a total of 18 ova recovered from the single-ovulated cows on 2-15 days after estrus, the rate of normal and abnormal ova was 55.6 and 44.4%, respectively. The cleavage stage of embryos was 32 cell morula on 9-12th days and elongated hatched blastocyst on 15th days. 9. The successful recovery rate of ova from the cow which catheter was fixed in its uterus body was 83%, while that from the cow which catheter was fixed in uterus horn was 83% There was no difference between uterus body and uterus horn. The recovery rate of ova with massage was 91.7% while that of ova without massage was 0.0% The ova couldn't be recovered without massage while flushing uterus. 10. When fertilized ova from KNC donors were tranferred to 3 Holstein and 2 KNC recipients, all the recipient were coming into heat and non-pregnant. 11. For the induction of twin, 2 Holstein cows which were in natural heat and inseminated were transfered with the ova from KNC and Hereford donors and were conceived, but calved single.