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피지선세포에서 SREBP-1 활성에 의한 우방자추출물의 지질 생성 촉진 작용
인명희,문연자,우원홍 한의병리학회 2020 동의생리병리학회지 Vol.34 No.1
Arctii Lappa Fructus has the numerous health benefits, including antioxidant, anti-inflammatory, and anti-carcinogenic properties. Skin lipids are one of several factors that maintain epidermal barrier function. This study was to explore the lipogenic effect by ethanol extract of Arctii Lappa Fructus (EAF) in sebocytes. First, it was confirmed that EAF exhibited high antioxidant activity and collagenase activity inhibition. We found that cholesterol and triglyceride levels of cells by EAF were increased significantly in a dose-dependent manner. Moreover, EAF increased the expression of transcription factor sterol regulatory element-binding protein-1 (SREBP-1) in the cells. These results suggest that EAF induces lipogenesis in cells through the activation of SREBP-1.
최형욱,인명희,리순화,김은주,임규상,우원홍,Choi, Hyung-Wook,In, Myung-Hee,Lee, Shun-Hua,Kim, Eun-Joo,Lim, Kyu-Sang,Woo, Won-Hong 대한한방안이비인후피부과학회 2015 한방안이비인후피부과학회지 Vol.28 No.4
Objective : This research has been conducted to comparative analysis of anti-inflammatory effects to suggest a usefulness of ethanol extracts fromGentianae sino-ornata(GSO) as a functional material for inflammatory activities.Methods : Cell viability was measured by neutral red (NR) assay, and nitric oxide (NO) production in RAW264.7 cells was monitored by measuring the nitrite content in culture medium. The expressions of cyclooxigenase-2 (COX-2) was determined by western blot analysis, and Inducible nitric oxide syntase (iNOS) and cytokine were determined by reverse transcriptase-polymerase chain reaction (RT-PCR).Results : When the GSO extract was added the concentration of 5-20 ㎍/㎕, the viability of cells was maintained 90% or more at all levels. NO production was suppressed by the treatment of GSO in LPS-stimulated RAW264.7 cells. GSO inhibited the expression of iNOS, COX-2, IL-1βand IL-6 in LPS-stimulated RAW264.7 cells.Conclusions: From this results, we consider that GSO can be a useful therapeutic and preventive approach to various inflammatory diseases as a functional material for inflammatory activities.
매괴화(??花) 에탄올추출물이 α-MSH로 유도된 과색소 형성 억제와 작용기전 연구
이진호,인명희,강석훈,문연자,우원홍,임규상,Lee, Jin-Ho,In, Myung-Hee,Kang, Suk-Hoon,Mun, Yeun-Ja,Woo, Won-Hong,Lim, Kyu-Sang 대한한방안이비인후피부과학회 2015 한방안이비인후피부과학회지 Vol.28 No.1
Objective : This study investigated the inhibitory mechanism of the hypopigmentating effects on ethanol extract of Rosae rugosae Flos (ERR) that has not yet been examined. Methods : We analyzed the anti-melanogenic effects of ethanol extracts from Rosae rugosae Flos by tyrosinase activity, melanin contents. We also examined protein expression levels of tyrosinase, TRP-1, TRP-2, MITF and ERK by western blot analysis in melanoma cells. Results : In this investigation, ERR effectively reduced ${\alpha}$-MSH-stimulated melanin synthesis by suppressing expression of tyrosinase and tyrosinase-related protein-1 (TRP-1). On the other hand, the expression of tyrosinase-related protein-2 (TRP-2) were not affected by treatment with ERR. ERR inhibited the expression of microphthalmia-associated transcription factor (MITF) as a key transcription factor for tyrosinase expression regulating melanogenesis. The upstream signaling pathway including cAMP response element-binding protein (CREB) and MAPKs were also inhibited by ERR. Pretreatment with PD98059, ERK inhibitor, attenuated the inhibitory effect of ERR on ${\alpha}$-MSH-induced tyrosinase activity. Conclusions : Our study suggested that the anti-melanogenic activity of ERR is correlated with the suppression of tyrosinase gene through CREB/MITF/ERK pathway.
밀싹 추출물의 ERK와 Akt 인산화를 경유한 인간 모유두세포 증식 촉진효과
박설아(Seol a Park),인명희(Myoung hee In),우원홍(Won hong Woo),문연자(Yeun ja Mun) 대한약학회 2017 약학회지 Vol.61 No.3
We investigated the effect of Wheat Sprout MeOH extract (WSM) on cell proliferation, and analyzed the molecular mechanism in human hair dermal papilla cells (HHDPCs). After exposure to WSM in HHDPCs, cell proliferation was increased compared with minoxidil-treated cells. Treatment with WSM led to phosphorylation of extracellular signal-related kinase (ERK) and Akt. Moreover, WSM-mediated increase of cell proliferation was attenuated by PD98059, ERK inhibitor, and LY294002, Akt inhibitor. These results suggest that WSM induced cell proliferation in HHDPCs through activation of ERK and Akt.
최형욱(Hyung Wook Choi),인명희(Myung Hee In),문연자(Yeun Ja Mun),임규상(Kyu Sang Lim),우원홍(Won Hong Woo) 한의병리학회 2015 동의생리병리학회지 Vol.22 No.2
In this study, we investigated the effect of ethanol extracts from Gentianae scabrae bunge (GSB) on the activities of antioxidant, whitening and anti-inflammation. Viability of cells was measured by neutral red (NR) assay, and inhibitory effects of GSB on melanin synthesis was determined the melanin production in B16F10 cells. The expression level of matrix metalloproteinase-1 (MMP-1) in media was analyzed by ELISA kit, and nitric oxide (NO) production in RAW264.7 cells was monitored by measuring the nitrite content in culture medium. GSB showed highly efficacy in 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, and significantly reduced melanin synthesis in B16F10 cells. MMP-1 production in UVB-stimulated human dermal fibroblast (HDF) cells was inhibited by GSB treatments. NO production was suppressed by the treatment of GSB in LPS-stimulated RAW264.7 cells. From this results, it was indicated that GSB could be utilized as anti-aging and whitening cosmetic ingredients.