현장 : 군선교이야기 : 나의 사랑 푸른 제복의 장병들 !

인동철 기독교대한성결교회 활천사 1997 활천 Vol.522 No.-

Babesia gibsoni에 감염(感染)된 개의 혈청단백분획(血淸蛋白分劃)에 관한 연구

인동철,이주묵,채준석,차현성,Ihn, Dong-cheol,Lee, Joo-muk,Chae, Joon-seok,Cha, Hyeon-sung 대한수의학회 1991 大韓獸醫學會誌 Vol.31 No.1

To observe the changes of serum proteins according to the process of Babesia gibsoni(B gibsoni) infection, the babesia protozoa($10^8/kg$) were inoculated into the cephalic vein of healthy dogs. The serum proteins of experimentally infected dogs were separated by using cellulose acetate electrophoresis. The results obtained were as follows; 1. Cellulose acetate electrophoresis was fractionated to total 6 of bands such as, albumin, ${\alpha}_1$, ${\alpha}_2$, ${\beta}_1$, ${\beta}_2$ and $\gamma$-globulin. 2. The concentration of total protein was shown a decreasing tendency after B gibsoni infection. Albumin and A/G ratio were lowered through all periods of the infection, but they were not significant changes. 3. The level of ${\alpha}_1$-globulin was significantly(p<0.05) incresed in early stage of the infection. 4. The levels of ${\alpha}_2$ and total $\alpha$-globulin were shown highly significant decreases (p<0.01) through all periods of the infection. 5. The levels of ${\beta}_1$ and total $\beta$-globulin had highly significant changes (p<0.01) that was increased in early stage of infection and decreased later. 6. The level of $\gamma$-globulin was seen to be constantly increased through all periods of infection. It was a highly significant change (p<0.01). 7. Plasma protein: fibrinogen (PP:F) ratio was shown a temporally significant increase (p<0.05) following the decrease in early infection.

기고 -혹서기 사양관리

인동철,In, Dong-Cheol 대한양계협회 2015 월간 양계 Vol.47 No.6

Babesia gibsoni에 感染된 개의 血淸蛋白分劃에 관한 硏究

印東哲,李周默,蔡俊錫,尹昌模 全北大學校 附設 畜産開發硏究所 1991 畜産開發硏究報告 Vol.4 No.-

To observe the changes of serum proteins according to the process of Babesia gibsoni(B gibsoni) infection, the protozoa(10^8/㎏) were inoculated into the cephalic vein of healthy dogs. The serum proteins of experimentaly infectied dogs were analyzed by cellulose acetate electrophoresis. The results obtained were as follows ; 1. The electrophoretic pattern of serum protein revealed total 6 of bands such as, albumin, α_1 and α_2-globulin, β_1 and β_2-globulin and γ-globulin. 2. Total serum protein showed a decreasing tendency after B gibsoni infection. Albumin and A/G ratio maintained low levels during the period of the infection, although they were not significant. 3. The levels of α_1-globulin significantly increased (P<0.05) in the early stage of the infection. 4. The levels of α_2-globulin and total α-globulin showed highly significant decreases (P<0.01) during the period of the infection. 5. The levels of β_1-globulin and total β-globulin were highly significant increse (P<0.01) in the early stage of infection and decreased later. 6. The γ-globulin constantly increased during the period of the infection (P<0.01). 7. Plasma protein : Fibrinogen (PP : F) ratio revealed a temporally significant increase (P<0.05) after decrease in the early infection.

법제유황의 실용적 제조에 따른 물리 화학적 분석 및 독성, 항균 작용에 관한 연구

인동철 ( Dong Chul In ),유도현 ( Do Hyeon Yu ),박철 ( Chul Park ),박진호 ( Jin Ho Park ) 한국동물위생학회 2012 韓國家畜衛生學會誌 Vol.35 No.3

Despite of a long history of the sulfur on the disease healing effect, there were limited ways of applying sulfur to animal and human. We have developed the detoxified sulfur (non toxic sulfur) method to make it practical and mass production possible through laboring for many years. This study practiced scanning electron microscope (SEM), Energy dispersive X-ray spectrometer (EDS) and secondary ion mass spectrometry (SIMS) analysis to investigate the physicochemical aspect of detoxified sulfur. We also performed the oral toxicity experiment to mice, and anti-bacterial test of the detoxified sulfur. Based on the SEM, EDS and SIMS results, the united particles in the mass form with the similar component intensity with the raw sulfur were observed, and hydrogen sulfide ion (HS-) component which is regarded as a toxic matter, was decreased after detoxification. Indeed, toxicity test on the mice (10 males, 10 females) showed no clinical, histopathological changes with the 5 times amount (2,500 mg/kg) of the actual doses. However, the male-mice showed decreased in body weight by 23.6%, 24.3% in the 7th, 14th day, respectively, after detoxified sulfur. Moreover, the female-mice administered the detoxified sulfur showed decreased in body weight by 28.7% (P<0.05) than that in the control group on the 14th day. The result of antibacterial test on the detoxified sulfur showed antibacterial effect (27%) to inhibit the growth of Staphylococcus aureus. It is shown that detoxified sulfur can be used as feed additive and has an affect on the farm perfomance.

개의 Babesia gibsoni 감염예방에 관한 연구 1. 항원의 Sonication 및 Formalin 처리에 의한 예방접종

채준석,인동철,이주묵,윤창모,Chae Joon-Seok,Ihn Dong-Cheol,Lee Joo-Muk,Yoon Chang-Mo 한국임상수의학회 1990 한국임상수의학회지 Vol.7 No.1

To examine the effects of vaccination against Babesia gibsoni infection in dogs, 15 normal mixed-breed dogs(5 month to 1 year old) divided into 3 groups with 5 dogs in a group. One of them was selected as control group(group A) and others were selected as experimental groups(group B and C). The group B was vaccinated with sonicated antigens and the group C was vaccinated with 0.2% of formalin treated antigens. The results obtained in the examination were summarized as follows : 1. In the western blot, the lane A revealed specific two bands on the regions of 54kd and 100kd, respectively. 2. After the first vaccination, the antibody titers of group B and C were higher 5 times(1 : 200) than those of control group(1:40). After the second vaccination, the antibody titers of group B and C have not changed. When challenged with the protozoa(Babesia gibsoni), the antibody titers(1 : 5,000) were elevated in all groups. But these were not exceeded over 1 : 5,000 for 4 weeks. 3. After challenge, the peak time of increased numbers of the protozoa was the 15th day (12-18 days) in all groups. During these days, the rate of parasitized erythrocytes in control group was 55.0${\pm}$5.4%. But those of group B and group C were 26.0${\pm}$6.4%, and 15.6${\pm}$7.8%, respectively. 4. After challenge, all of the values of PCV, Hb, RBC were shown to decrease in all of the control and experimental groups. 5. The total leukocytes counts are shown a tendency of reduction in all groups after challenge. 6. In all groups, there were increase in lymphocytes and monocytes after challenge.

Caine Babesia spp. 감염증예

채준석,인동철,한재철,김남수,이주묵,최인혁,Chae Joon-Seak,Ihn Dong-Chul,Han Jae-Chul,Kim Nam-Soo,Lee Joo-Muk,Choi In-Hyuk 한국임상수의학회 1989 한국임상수의학회지 Vol.6 No.1

A dog which was hospitalized to Veterinary Teaching Hospital, College of Veterinary Medicine, Jeonbug National University on December 28, 1988 was revealed severe anemia: hemoglobinuria and weakness. In the inspections, abdominal pain and spleno megaly at the ventral abdomen were detected by palpations. In the examinations of blood, the obtained results were summarized as follows: Babesla spp. was identified on the blood smear stained with Giemsa. The Babesia spp. was assumed to the Babesia gibsoni for the their small size and pleomorphism such as comma form, ring form and dot form. In the blood examinations of the patient, Ht: 22.5%, RBC:354${\times}$10$^4$/${\mu}\ell$, Hb: 8.8g/dl, serum protein: 8g/dl, and WBC count was 21, 425/${\mu}\ell$. In the chemical examinations of serum, the value of AST(GOT) was 30iu and ALT(GPT) was 20iu, respectively. The blood sugar was 60mg/d1. In the urine test, urine protein was 30mg/d1 and the hemoglobin In the urine was the +++ and occult blood reaction(Benzidine test) in the feces was +++. Splenomegaly was confirmed by X-ray examination. To confirm for the Babesia spp. infection, 5ml of the whole blood of the patient(3% of Parasitized erythrocytes) were inoculated into the cephalic vein of the two normal dogs. In the blood of experimental dogs which were inoculated parasitized blood, Babesia spp. was detected in the two doss and pleomorphic parasites were observed, too. In the blood examinations of No. 1 the Ht and RBC were decreased to 6.8% and 52${\times}$10$^4$/${\mu}\ell$, respectively. WBC count was 10.600/${\mu}\ell$ and serum protein was 6.8g/dl. The rates of parasitized erythrocytes were 15% in the experimental dog. Also +++ of the hemoglobin was detected in the urine. In the X-ray examination, splenomegaly was comfirmed and it was confirmed by autopsy of the experimental dog(No. 1).

Development and Evaluation of a SYBR Green Real-time PCR Assay for Canine Cytokine Gene Expression

유도현,인동철,박철,박진호 한국임상수의학회 2010 한국임상수의학회지 Vol.27 No.5

Cytokines are important mediators of the immune response, and quantitating cytokine mRNA is a highly sensitive and attractive method for measuring cytokine production. The objective of the current study was to develop and validate a SYBR green quantitative real-time reverse transcriptase PCR (qRT-PCR) assay for measuring canine cytokine mRNA. The optimal annealing temperatures (Ta) of the designed primers were 62oC for interleukin (IL)-1β, IL-6 and IL-10; 60oC for glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and tumor necrosis factor (TNF)-α; and 58oC for high mobility group box 1 (HMGB1). Primer efficiencies of all primers calculated for standard curve samples were between 97.1% and 102.6%. No evidence of secondary structure or primer-dimer formation was seen via melt-curve analysis or gel electrophoresis. The developed qRT-PCR assays are highly specific and sensitive and can be used to quantify gene expression levels of canine cytokines.

산삼배양액을 급여한 돼지에서 근육의 프로테옴 분석

설재원,채준석,강형섭,강춘성,인동철,박상열 한국임상수의학회 2011 한국임상수의학회지 Vol.28 No.1

Proteomics is a useful approach to know protein expression, post-translational modification and protein function. We investigated the protein expression pattern and identity in pigs fed with the tissue culture medium waste after harvest of Korean wild ginseng (TCM-KWG) (Panax ginseng). Two groups (n = 30/group) of pigs were administered with 0 (control) and 16 ml/L (treatment) TCM-KWG through drinking water. After 4 weeks, we examined the protein expression pattern of longissimus dorsi muscle by Two-dimensional electrophoresis analysis. TCM-KWG treatment significantly increased two spot's density, and markedly reduced one spot's density in the muscles. We identified 3 proteins (heat shock protein 90-alpha, myosin binding protein and cofilin 2) by the ESI-MS/MS (Q-TOF2, Micromass). These results demonstrate that TCM-KWG treatment may play a protection role against physiological stress in pigs,like as increased heat shock protein 90-alpha.