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Development and Evaluation of a SYBR Green Real-time PCR Assay for Canine Cytokine Gene Expression
유도현,인동철,박철,박진호 한국임상수의학회 2010 한국임상수의학회지 Vol.27 No.5
Cytokines are important mediators of the immune response, and quantitating cytokine mRNA is a highly sensitive and attractive method for measuring cytokine production. The objective of the current study was to develop and validate a SYBR green quantitative real-time reverse transcriptase PCR (qRT-PCR) assay for measuring canine cytokine mRNA. The optimal annealing temperatures (Ta) of the designed primers were 62oC for interleukin (IL)-1β, IL-6 and IL-10; 60oC for glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and tumor necrosis factor (TNF)-α; and 58oC for high mobility group box 1 (HMGB1). Primer efficiencies of all primers calculated for standard curve samples were between 97.1% and 102.6%. No evidence of secondary structure or primer-dimer formation was seen via melt-curve analysis or gel electrophoresis. The developed qRT-PCR assays are highly specific and sensitive and can be used to quantify gene expression levels of canine cytokines.
유도현,You, Do-Hyun 대한전기학회 2006 전기학회논문지C Vol.55 No.7
[ $TiO_2-Nb_2O_5$ ] sol was prepared using sol-gel method. Crystalline properties of gel powder changed from rutile phase to anatase phase with increasing $Nb_2O_5$ additives at $800^{\circ}C$, while they retained rutile phase regardless of $Nb_2O_5$ additives at $900^{\circ}C,\;1,000^{\circ}C$. They retained amorphous phase from $50^{\circ}C\;to\;400^{\circ}C$, retained anatase phase from $500^{\circ}C\;to\;600^{\circ}C$ and had rutile phase over $700^{\circ}C$ at 1mole% $Nb_2O_5$ additive. After $TiO_2-Nb_2O_5$ sol retained low viscosity with normal chain structure for a long time, its viscosity increased fast with network structure. DTA properties of gel powder had endothermic reaction due to evaporation of propanol and water about $78^{\circ}C$, had exothermic reaction due to propanol combustion about $290^{\circ}C$ and had exothermic reaction due to changing of $TiO_2$ phase about $640^{\circ}C$.
CD11b as a Biomarker for Canine Systemic Inflammatory Response Syndrome and Sepsis
유도현,노동호,송루희,김준환,이다미,김수희,박철,박진호 한국임상수의학회 2010 한국임상수의학회지 Vol.27 No.6
The aim of this study is to investigate neutrophil activation markers among canine ICU (intensive care unit) control, systemic inflammatory response syndrome (SIRS) and sepsis. These markers include WBC (white blood cells), platelets counts, blood film examination (neutrophilic band to segmentation ratio and neutrophilic degenerative changes), and flow cytometric analysis (CD11b expression of neutrophils). As a result, the mean CD11b fluorescence intensity. of neutrophils and the neutrophilic degenerative change scores were both significantly higher in sepsis group (P < 0.05). In addition, mortality was also found to be correlated with the up-regulation of CD11b expression in circulating neutrophils. This study demonstrates that CD11b expression of neutrophils could be more a reliable biomarker to predict prognosis in ICU patients than traditional blood film examination according to this study.
유도현 대한전기학회 2006 전기학회논문지C Vol.55 No.7(C)
- TiO2-Nb2O5 sol was prepared using sol-gel method. Crystalline properties of gel powder changed from rutile phase to anatase phase with increasing Nb2O5 additives at 800℃, while they retained rutile phase regardless of Nb2O5 additives at 900℃, 1,000℃. They retained amorphous phase from 50℃ to 400℃, retained anatase phase from 500℃ to 600℃ and had rutile phase over 700℃ at 1mole% Nb2O5 additive. After TiO2-Nb2O5 sol retained low viscosity with normal chain structure for a long time, its viscosity increased fast with network structure. DTA properties of gel powder had endothermic reaction due to evaporation of propanol and water about 78℃, had exothermic reaction due to propanol combustion about 290℃ and had exothermic reaction due to changing of TiO2 phase about 640℃.
Leukocyte Markers Differentiate Non-Infected from Spontaneously Infected Dairy Cows
유도현,이종현,송루희,노동호,이영화,이미진,박진호 한국임상수의학회 2009 한국임상수의학회지 Vol.26 No.6
Spontaneously infected and non-infected dairy cows were assessed in a cross-sectional study aimed at determining whether bovine leukocyte markers may diagnose intra-mammary infections (bovine mastitis). Animals located in herds where bovine mastitis was highly prevalent were investigated (n = 31 animals). The expression of three cell-surface markers (CD11b, CD4 and CD8) was assessed, and the somatic cell count (SCC) and bacteriological analyses (both cultures and PCR tests) were also conducted. Cows identified as infected revealed statistically significant higher milk leukocyte CD11b, CD4 percentage and milk CD4/CD8 ratios than non-infected cows. Immunological markers may diagnose spontaneous bovine mastitis.
솔젤법에 의해 제작된 TiO2 솔과 SiO2 솔의 점도 특성에 대한 분석
유도현 대한전기학회 2005 전기학회논문지C Vol.54 No.12-C
TiO2 sol and SiO2 sol were prepared using sol-gel method. As H2O/Alkoxide ratios increased, sol had cluster structure and as H2O/Alkoxide ratios decreased, sol had linear structure. Gelation time of TiO2 sol was faster than that of SiO2 sol according to the time. In comparison with initial viscosity between TiO2 sol and SiO2 sol, TiO2 sol was highest at H2O/Ti(OC3H7)4=5, SiO2 sol was almost constant according to H2O/Si(OC2H5)4 ratios.
Detection of Canine Lymphoma by the Amplification of Antigen Receptor Gene Rearrangements
유도현,이영화,이종현,노동호,송루희,이미진,최을수,박진호 한국임상수의학회 2009 한국임상수의학회지 Vol.26 No.5
We performed the PARR (PCR to detect antigen receptor rearrangements) test on DNA isolated from twelve archival canine cytological slides including nine lymphoma, two reactive lymphocytes and one sample from Ehrlichia canis infected dog. As a result, our PCR control gene, Cμ, was successfully amplified from all of the DNA samples. Six out of nine lymphoma samples showed a clonal rearrangement of immunoglobulin gene whereas three samples did a clonal rearrangement of T cell receptor gamma (TCRγ) gene. However, we observed no visible or clear bands from PCR conducted using our antigen receptor rearrangement primers on DNA from a reactive lymphoid cell proliferation used as a negative control. False-positive amplification in TCRγ gene was observed only in one sample from E. canis infection. The use of archival cytological specimens demonstrated in this study offers potential advantages for cost-effective specimen acquisition and efficient high-fidelity DNA analysis.