부산지역 하천에서 분리된 장내세균 Escherichia coli와 Klebsiella pneumoniae의 광범위 베타 락탐 분해효소 (Extended-Spectrum ${\beta}-Lactamase$)에 대한 유형별 분류

이훈구,김혜진,김군도,Lee, Hun-Ku,Kim, Hye-Jin,Kim, Gun-Do 한국미생물학회 2007 미생물학회지 Vol.43 No.2

본 연구는 부산지역 하천에서 plasmid 매개성 광범위 ${\beta}-lactam$ 분해효소(extended spectrum ${\beta}-lactamase;\;ESBL$)를 생성하는 세균을 분리하여 생성된 광범위 ${\beta}-lactam$ 분해효소를 유형별로 분류하기 위함이다. Escherichia coli 6균주와 Klebsiella pneumoniae 15균주가 피전달균주인 Escherichia coli J53 $Azid^{R}$에 plasmid 매개성 광범위 ${\beta}-lactam$ 분해효소 생성 인자를 전달하였다. PCR 후 얻은 유전인자를 plasmid로 매개된 광범위 ${\beta}-lactam$ 분해효소 유전자의 염기서열 분석결과, E. coli와 K. pneumoniae 유전자를 BCM Search Launcher 및 GenBank nucleotide database를 통하여 검색한 결과 ESBL 유형은 TEM-52형과 SHV-12형이었다. TEM-52는 E. coli와 K. pneumoniae 양쪽에서 분리되었다. 그러나 SHV-12는 K. pneumoniae에서만 분리되었다. 이상의 결과로부터 plasmid 매개성 광범위 ${\beta}-lactam$ 분해효소를 생성하는 세균이 한국에서는 이미 임상범위를 넘어 자연계까지 확산되었음을 알 수 있었다. The purpose of this study was typing the plasmid mediated extended spectrum ${\beta}-lactamases$ produced by enteric bacteria isolated from rivers in Pusan. Six strains of Eschericha coli and fifteen strains of Klebsiella pneumoniae transferred their plasmid mediated extended spectrum ${\beta}-lactamase$ genes to the recipient strain Eschericha coli J53 $Azid^{R}$. The plasmid mediated extended spectrum ${\beta}-lactamase$ genes were sequenced directly after PCR and the types were determined by the BCM Search Launcher and GenBank nucleotid database. Determined types of the plasmid mediated extended spectrum ${\beta}-lactamases$ were TEM-52 and SHV-12. TEM-52 was isolated from both Escherichia coli and Klebsiella pneumoniae. However SHV-12 was isolated from Klebsiella pneumoniae only. The results indicated that the plasmid mediated extended spectrum ${\beta}-lactamase$ producing bacteria spreded over the area of clinical to the nature in Korea.

Types of Extended-Spectrum β-Lactamase Produced in Enteric Bacteria Isolated from Sewage Plant Drain Water

Gun-Do Kim(김군도),Hun-Ku Lee(이훈구) 한국생명과학회 2010 생명과학회지 Vol.20 No.5

본 연구는 임상검체만이 아닌 주변 생활환경에도 광범위 베타 락탐분해효소를 생성하는 균주(extended-spectrum β-lactamase, ESBL)가 존재하는지를 확인하고 만약 존재할 경우 그 균주를 분리하고 ESBL유형을 알아보기 위하여 실시되었다. 부산 광안리 하수처리 방류수에서 이중 디스크 확산 검사 결과 양성반응을 나타낸 29균주를 선별하였다. 이중 sodium azide에 내성을 가진 피전달 균주인 Escherichia coli J5에 교차접합이 이루어진 15균주를 대상으로 indole, methyl-red, Voges-Proskauer, Simmon's citrate 시험과 decarboxylase-dihydrolase 및 여러 종류의 당 발효 시험 등 생화학 검사를 실시한 결과 Klebsiella pneumoniae(13균주)와 Escherichia coli(2균주)가 동정되었다. 등전점, PCR, 유전자서열 분석을 실시하여 ESBL 유형을 결정하였다. Klebsiella pneumoniae의 ESBL 생성유형은 SHV-12(4균주)와 SHV-12/TEM-1(9균주)의 2종류로 구분되었고, Escherichia coli의 ESBL 생성유형은 TEM-1(2균주)로 판명되었다. This study focused on typing of the extended-spectrum β-lactamase (ESBL) produced in organisms isolated from a natural environment, rather than a clinical setting. Samples were collected from drain water issuing from a sewage plant in Kwanganri (Busan, Korea). Following double disk synergy testing, 29 strains were selected as potential ESBL positive strains. Of these, 15 strains were transconjugants of the sodium azide resistant recipient strain Escherichia coli J53 and analyzed biochemically including indole, methyl-red, Voges-Proskauer, Simmon's citrate, decarboxylase-dihydrolase and sugar- fermentation tests. The tests classified the 15 strains as Klebsiella pneumoniae (n=13) and Escherichia coli (n=2). The type of ESBL from each strain was deduced by isoelectric focusing point analysis and DNA sequencing. The results indicated that the types of ESBL were SHV-12 (n=4) and SHV-12/TEM-1 (n=9) from K. pneumoniae and TEM-1 (n=2) from E. coli strains.

부산 수영공공하수처리시설에서 분리된 광범위 항균제 베타락 탐 분해효소(Extended-Spectrum ${\beta}$-Lactamase, ESBL) 유형

김군도,이훈구,Kim, Gun-Do,Lee, Hun-Ku 한국미생물학회 2010 미생물학회지 Vol.46 No.1

본 연구는 부산 환경관리공단 수영사업소 산하 수영공공하수종말처리장 하수로부터 광범위 베타락탐 분해효소(ESBL, extended-spectrum ${\beta}$-lactamase)의 유형을 파악하기 위하여 이루어졌다. 수영공공하수처리시설은 부산광역시의 동북부 생활하수와 수세식변기의 오수가 표준활성슬러지공법으로 처리되며 일 생활하수 처리량은 총 550,000톤이다. 이중디스크 확산 검사, 제 3세대 세파계열 항균제에 대한 최소억제농도 시험을 통하여 14균주를 선별하였다. Indole, methyl-red, Voges-Proskauer, Simmon's citrate, decarboxylase-dihydrolase 시험과 당 발효 시험 등 생화학 검사를 통하여 동정한 결과 Escherichia coli (10균주), Klebsiella pneumoniae (4균주)가 동정되었다. 이를 전달균주로, sodium azide에 내성을 가진 피전달 균주인 E. coli J53에 교차접합을 시켜 11균주 (E. coli 9균주, K.pneumoniae 2균주)가 접합이 이루어졌다. 접합자중 2균주는 ESBL 유전자를 전달받지 못하였고 9균주는 부모로부터 ESBL 유전자를 전달받았다. 등전점, 유전자서열과 단백질서열 분석 등을 통하여 피전달균주인 E. coli J53에 전달된 유전자유형은 TEM형의 모형인 TEM-1과 SHV-12형으로 규명되었다. The study performed to identify the type of ESBL against strains which are producing extendedspectrum ${\beta}$-lactamases and isolated from sewage in Suyeong sewage disposal plant, Busan Environmental Corporation. By the standard activated sludge method, Suyeong sewage disposal plant purify living and lavatory sewage gathering from the northeast Busan and the facility purify total 550,000 tons of living sewage disposal a day. 14 strains were isolated by double disk synergy test and the third generation cepha-antibiotics test. Indole, methyl-red, Voges-Proskauer, Simmon's citrate, decarboxylasedihydrolase and sugar-fermentation tests identified as Klebsiella pneumoniae (n=4) and Escherichia coli (n=10). Plasmid-mediated transmission test against isolated 14 strains proved 11 strains transmitted resistance to recipient E. coli J53 (sodium $azide^R$, $ceftazidime^S$). 9 strains of conjugant were expressed ESBL genes transferred from parental strain but 2 conjugants did not expressed. The type of ESBL from each strain was determined by isoelectric focusing points, DNA and amino acids sequencing. The results indicated that the types of ESBL transmitted to recipient E. coli J53 were TEM-1, the parental TEM type and SHV-12 type.

교류 전기철도 급전계통 4도체군 전차선로 분석 및 FDTD 방법을 이용한 선로 주변 유도전압 계산에 관한 연구

류규상(Kyu-Sang Ryu),염형선(Hyoung-Sun Yeom),조규정(Gyu-Jung Cho),이훈도(Hun-Do Lee),김철환(Cheol-Hwan Kim) 대한전기학회 2016 전기학회논문지 Vol.65 No.12

AC railway feeding system use single phase to supply power to the railway vehicles. And the system use the rail track as a return current path, so that current flows in the rail. In this situation inductive interference on communication system and unsafe environment can appear on railway system. Therefore knowing the current distribution of catenary system and analysing the return current is required. In this study detail return current distribution was analyzed by modeling the catenary system as 4-conductors group. The distribution characteristics and trends of return current were studied by using the PSCAD/EMTDC and FDTD method that based on Maxwell equation was used to calculate the induced voltage. Simulation code was made by MATLAB. Using this study result data, we can suggest the proper installation location of digital device and these data can be used for additional studies related to return current or induced voltage as a base data.

단선 AT 급전계통의 전차선-레일 단락 고장전류 분석

조규정(Gyu-Jung Cho),류규상(Kyu-Sang Ryu),이훈도(Hun-Do Lee),허승훈(Seung-Hun Heo),김철환(Chul-Hwan Kim),권성일(Sung-Il Kwon),김철환(Cheol-Hwan Kim) 대한전기학회 2015 전기학회논문지 Vol.64 No.12

In general, AT(Auto-transformer) is used to improve voltage sag and inductive interference in power lines and communication lines in AC electrical railway feeding system. Especially, Korean AT feeding system has different composition compared to other countries like Japan or France, so that it has some special characteristics. However, relays imported from other countries have been used in Korea, and settings of these relays should be modified and reflect distinct characteristics of Korean AT feeding system. Therefore, study about fault analysis based on modeled AT feeding system is important. In this paper, we modeled a single track AT feeding system by using PSCAD and analyzed fault current flow in case of trolley-rail short circuit fault. Finally, we presented current magnitude of each branch expressed by boosting current of AT.

백서의 급성폐손상에서 surfactant의 항염증작용과 호중구의 NK-κ B 활성과의 관계

안창혁 ( An Chang Hyeog ),차영주 ( Cha Yeong Ju ),이경희 ( Lee Gyeong Hui ),유철규 ( Yu Cheol Gyu ),이병준 ( Lee Byeong Jun ),정도영 ( Jeong Do Yeong ),이훈 ( Lee Hun ),신종욱 ( Sin Jong Ug ),김재열 ( Kim Jae Yeol ),박인원 ( Park 대한결핵 및 호흡기학회 2002 Tuberculosis and Respiratory Diseases Vol.53 No.5

광복 후 한국야학의 유형과 교육사적 의의

이훈도 한국교육철학회 1997 교육철학 Vol.15 No.-