FK506이 T 림프구 사멸에서 활성산소 생성에 미치는 영향

이호균(Ho Kyun Lee),정상영(Sang Young Chung),최수진나(Soo Jin Na Choi) 대한외과학회 2009 Annals of Surgical Treatment and Research(ASRT) Vol.77 No.5

Purpose: Tacrolimus (FK506) has been widely used as an immunosuppressant in organ transplanted recipients to suppress organ rejection phenomenon. We investigated the role of oxidative stress and heme oxygense-1 by FK506 on human Jurkat T cells. Methods: The cells viability was examined by DAPI stain, enzyme activity of caspase family proteins, and western blotting for Baks, PUMA, iNOS, HO-1. Cells were cultured in the absence or presence of CoPPIX or ZnPPIX and the fluorescence intensity was analyzed using a flow cytometry. Results: Treatment with FK506 increased the generation of reactive oxygen species (ROS), including hydrogen peroxide and superoxide anion, and NO in Jurkat cells in a dose-dependent manner. Immunohistochemistry and Western blot analysis data revealed the hemoxygenase-1 (HO-1) was induced by the addition of FK506 in Jurkat cells. Induction of CoPP, HO-1 inducer, resulted in decreased intracellular H₂O₂ and NO concentrations. Instead ZnPP, an HO-1 competitive inhibitor did it reversely. In addition, ZnPP regulates iNOS protein synthesis by inhibition of HO-1. Conclusion: Increase of HO-1 expression would induce to decrease the intracellular H₂O₂ and NO concentrations. Also, HO-1 would regulate iNOS protein synthesis. Consequently, we can expect the regulation of HO-1 expression with concomitants use of FK506 to suppress organ rejection phenomenon by enhancing apoptosis.

이호정 교수 근영

이호정 ( Lee Ho Jeong ) 서울대학교 법학연구소 2002 서울대학교 法學 Vol.43 No.1

허브 형상에 따른 정풍량 환기팬의 유동과 성능특성

이호호(Ho Ho Lee),최항철(Hang Cheol Choi),정재구(Jae Goo Jung),이윤표(Yoon Pyo Lee),신유환(Yoo Hwan Shin),정진택(Jin Taek Chung) 한국유체기계학회 2011 유체기계 연구개발 발표회 논문집 Vol.2011 No.11

The constant air volume flow fan can maintain constant flow rate to the wide range of exit pressure. Therefore, the use of this fan is increasing recently for ventilation of high building. Brushless DC motor is adopted to this fan because that has advantages of compactness and performance. But this type of motor protrude from impeller hub side to fan inlet. The Impeller inlet flow is influenced by size of this obstacle called hub. In this paper, the influence of hub shape on the fan performance characteristics are experimentally and numerically analyzed. CFX 12.0 is used to perform the fan internal flow analysis and numerical results are compared with the experiments. Depending on hub shape, internal loss is generated and the performance and efficiency are reduced. The best performance is occurred around h/b1=0.25. The results of this study will be contribute to initial design of constant air volume flow fan development.

도서산림 관리를 위한 관련 법제도 고찰과 개선방안에 관한 연구

이호상 ( Lee¸ Ho-sang ),현창석 ( Hyun¸ Chang-seok ),이보라 ( Lee¸ Bo-ra ),이광수 ( Lee¸ Kwang-soo ),신순호 ( Shin¸ Soon-ho ) 한국도서(섬)학회 2021 韓國島嶼硏究 Vol.33 No.2

본 연구는 도서지역 산림 관리를 위해 현행 법·제도에 관한 고찰을 통하여 지속적인 도서지역의 산림 생태계 보전을 위한 도서산림관리 방향을 제시하는데 있다. 최근 국내외적으로 도서 생물다양성 보전 등에 대한 논의가 증가하고 있으며, 우리나라 도서(섬) 관리정책도 주민의 삶, 경제, 생태적 지속성 등을 고려하는 방향으로 변화되고 있다. 우라나라의 도서는 각 부처에 따라 그 수치가 상이하나 최근의 발간한 관련 서적과 행정안전부 및 해양수산부 발표에 따르면 2020년 4월 기준으로 총 3,383개(유인도서 465개, 무인도서 2,918개)로 파악되었으며, 산림의 분포하는 도서는 2,325개(69.5%)로 산림면적은 총 220,819㏊로 전국 산림면적의 약 3.5%를 차지하고 있다. 도서지역 관련하여 유인도서는 행정안전부와 국토교통부, 무인도서는 해양수산부, 특수도서는 환경부와 해양수산부 그리고 행정안전부가 법적 근거를 가지고 관리함으로써 도서 관리주체가 다원화 되어 있고, 주로 유인도서는 개발, 무인도서 및 특수도서는 보전적인 특징을 가지고 있다. 산림분야는 산림보호를 직접적인 대상으로 하는 「산림보호법」이 있고 그 밖에「자연환경보전법」 등과 같이 환경보전이나 생태계보전과 관련하여 산림을 간접적으로 보호하는 법체계를 가지고 있다. 도서지역에는 여타지역과 기후나 자연 생태적 환경이 다르고, 오랫동안 육지부와 격절되어 있는 삶의 여건에 의해 산림자원적 측면에서 독특한 특성을 가지고 있다. 그러나 이러한 산림자원은 근래 개발행위와 생태계 자체의 교란 등으로 인해 여러 가지 부정적 변화가 야기되고 있다. 도서지역의 산림보호는 섬 자체의 가치를 제고해 줄 뿐만 아니라 독특한 산림자원의 안정적 보호라는 측면에서 매우 중요하다. 본 논문에서는 이 같은 도서산림을 보호하는데 궁극적 목적을 두고 그 제도적 개선방안을 모색하고자 한다. 도서산림 관리를 위한 법제의 개선안으로 첫째, 현행 법령 등을 개정하는 방안과 ‘(가칭)도서지역 산림보전 및 관리에 관한 법률’을 제정하는 방안을 제시하고자 한다. 현행 법률 등의 개정(안)은 도서지역 산림관리 위주로 산림자원조성과 산림보호 및 예산을 강화하기 위한 규정만을 개정하는 안으로 가장 용이한 개선방안이라고 할 수 있다. ‘(가칭)도서지역 산림 보전 및 관리에 관한 법률’을 제정하는 방안은 도서산림 관리의 권한을 보다 명확하게 하여 부처간 충돌문제가 발생하지 않을 수 있다. 이를 통해 도서지역 산림관리의 실효성을 확보하고, 산림청이 도서지역에서도 산림의 체계적인 관리에 대한 책임을 지는 당위성을 확보할 수 있을 것이다. This study was undertaken to suggest the direction of island forest management for the conservation of the forest ecosystem in the island region through the review of the current legal system for forest management in the island area. Recently, there has been an increasing discussion on the conservation of island biodiversity both domestically and internationally, and the island management policy in Korea has also been changed to consider the life, economy, and ecological sustainability of local residents. The total number of islands that are geographically cut off from land and surrounded by the sea was 3,383(465 inhabited islands and 2,918 uninhabited islands), 2,325 (55.9%) distributed forests and 220,819ha forest area, which is estimated to account for about 3.5% of the nation's forest area. In relation to the island area, the principal agents in island management are diversified by managing the populated islands via the Ministry of the Interior and Safety, the uninhabited islands with the Ministry of Oceans and Fisheries, the Ministry of Environment, the Ministry of Oceans and Fisheries, and the Ministry of the Interior and Safety. Mainly, inhabited islands are developed while uninhabited islands and special islands have conservation characteristics. In the forest sector, other than the Forest Protection Act, which is directly subject to forest protection, forests are indirectly protected in connection with environmental conservation or ecosystem conservation, including by the Natural Environmental Conservation Act. The legal system that approaches the concept of island forest as a different concept rather than that of a collective is needed, as most of the current forest-related laws are applied to develop, use, preserve, and manage forests and mountainous areas. As a measure to improve the legal system for the management of island forests, first, it is intended to suggest a plan to revise the current laws and regulations and to enact the Act on the Conservation and Management of Forests on Islands (tentative name). The revision (proposal) of the current law is the easiest improvement plan by revising only the regulations to create forest resources, protect forests, and strengthen budgets, mainly for forest management in the island areas. The plan to enact the Act on the Conservation and Management of Forests in Island Areas (tentative name) may make the authority of the management of island forests clearer, so that no conflict between ministries may occur. Through this, it will be possible to secure the effectiveness of island forest management and to secure the necessity of the Forest Service to take responsibility for the systematic management of forests in the island areas.

국소배기시설의 덕트피팅 압력손실계수 예측

이호형 ( Ho Hyung Lee ),이승철 ( Seung Chul Lee ),옥윤용 ( Yun Yong Ok ),이승호 ( Seung Ho Lee ) 대한설비관리학회 2014 대한설비관리학회지 Vol.19 No.4

Traditionally, the coefficients have been obtained using experimental measurements according to AHSRAE Standard 120, a process which is time consuming and expensive. An alternative is to use CFD, but this has uncertainty due to the approximations used in modeling turbulence. This study estimated that the pressure loss coefficients of duct fitting(elbow, tee) by turbulence model(Standard k-ε, Realizable k-ε, RSM, SST k-ω) using CFD techniques. The results show that Realizable k-ε model could accurately predict the pressure loss coefficients from all cases.

수술후 7년에 재발한 췌장의 고형 유두상 상피종양

이호심(Ho Sim Lee),이성걸(Seong Geol Lee),이정선(Jeong Sun Lee),최창환(Chang Whan Choi),김용기(Young Ki Kim),김종숙(Jong Sook Kim),우제호(Je Ho Woo) 대한소화기학회 1991 대한소화기학회지 Vol.23 No.4

Solid and papillary epithelial tumor that occurs chiefly in young woman. It is amenable to surgical resection and cure. Recurrence after operation and distant metastasis are rare. But, a few cases with recurrence or distant metastasis have been reported. We have encounted a case of solid and papillary tumor of the pancreas with distant metastasis and recurrence 7 years after resection in 34 years old female, so report it.

허브 형상에 따른 정풍량 환기팬의 유동과 성능특성

이호호(Ho Ho Lee),최항철(Hang Cheol Choi),정재구(Jae Goo Jung),이윤표(Yoon Pyo Lee),신유환(Yoohwan Shin),정진택(Jin Taek Chung) 한국유체기계학회 2012 한국유체기계학회 논문집 Vol.15 No.2

Mycophenolic Acid에 의해 유도된 Jurkat 세포주 세포자멸사에서 Heme Oxygenase-1의 발현이 미치는 영향

이호균(Ho Kyun Lee),최수진나(Soo Jin Na Choi) 대한외과학회 2010 Annals of Surgical Treatment and Research(ASRT) Vol.78 No.6

Purpose: This study demonstrates that pharmacologic induction of heme oygenase-1 (HO-1) along with catalytic activation significantly modulated apoptosis of Jurkat cells induced by mycophenolic acid (MPA). Methods: Cells were cultured with the presence or absence of MPA. Flow cytometric analysis was performed after propidium iodide staining. Western blotting of HO-1, Bcl, and Bax was also performed. Cells were stained 4’-6-Diamidino-2-phenylindole (DAPI) and measured by flow cytometry in the absence or presence of CoPPIX. Results: Treatment of MPA decreased cell viability in a dose- and time-dependent manner. MPA-induced cell death was confirmed as apoptosis characterized by sub G0/G1 phase arrest. Expression of HO-1 assumes a pattern of decline after rising at the initial phase. CoPPIX, HO-1 inducer, significantly inhibited the cisplatin-induced apoptosis. Treatment of MPA resulted in reactive oxygen species (ROS) generation in Jurkat cells. CoPPIX attenuated ROS production in MPA-treated cells. Conclusion: This result suggests that the protective mechanism of HO-1 on MPA-induced cytotoxicity is associated with direct inhibition of ROS generation and mitochondrial permeability transition.

춘천 지역에서 발생한 쯔쯔가무시병의 임상적 특징

이호권 ( Ho Gwon Lee ),민슬기 ( Seul Ki Min ),공승진 ( Seung Jin Kong ),이수정 ( Su Jung Lee ),송헌호 ( Hun Ho Song ),윤종우 ( Jong Woo Yoon ),이명구 ( Myung Goo Lee ),신동훈 ( Dong Hoon Shin ),강성하 ( Sung Ha Kang ),이정열 ( Jeo 대한내과학회 2005 대한내과학회지 Vol.69 No.2

목적 : 쯔쯔가무시병 환자의 일부는 중증감염으로 중환자실 집중치료를 필요로 하며 이 중 사망하는 환자들도 있다. 본 연구는 춘천 및 인근 지역에서 발생한 쯔쯔가무시병 환자의 임상적인 특징과 이 중 중증환자들의 특징을 분석하여 지역사회의 쯔쯔가무시병의 진단과 치료 및 교육에 도움이 되고자 하였다. 방법 : 1997년 4월부터 2002년 12월까지 한림대학교 부속 춘천성심병원에서 쯔쯔가무시 병으로 진단되었던 81예의 환자들의 의무기록 열람을 통한 후향적 분석을 시행하였다. 81예 환자들의 내원시 임상소견을 분석하였고, 이 중 본 병원에서 치료 받았던 79예의 환자들의 치료결과를 분석하였으며, 내원시 일반병실로 입원한 환자와 중환자실로 입원하였던 환자의 임상소견을 비교하여 중증환자들의 특징을 비교하였다. 결과 : 73예의 환자가 가을에 발생하였으며, 70예에서 발열이 있었고, 가피가 발견된 경우는 58예(71.6%)였다. Doxycycline 치료 후 45예의 환자가 48시간내 열이 없어졌으나 13예에서는 5일이상 열이 지속되었다. 79예 중에서 중환자실로 입원한 경우가 25예 였으며 이 중 8예에서 기계환기 치료를 받았고, 3예가 다발성 장기부전으로 사망하였다. 중환자실에 입원한 중증환자들은 백혈구수가 많았고, BUN과 크레아티닌이 높았고, 알부민 수치가 낮았다. 결론 : 쯔쯔가무시병은 중증질환으로 이환될 수 있으며 특히 고령이거나 만성질환을 가지고 있는 환자에게서는 치명적인 결과를 초래할 수 있으므로 감염발생의 위험이 있는 지역사회에서 유행 시기에 발열 등의 증상이 있을 때 조기 진단이 필수적이며 이를 위한 교육이 필요하다. 그리고 중증환자에서 백혈구수, 혈청 알부민, BUN, 크레아틴 등에서 차이를 나타내어 쯔쯔가무시 병의 예후를 예측하는 요소로 사료된다. Background : Tsutsugamushi disease is an acute febrile disease in Korea. Some patients with tsutsugamushi disease have severe illness, which needs care in intensive care unit, and may die due to the disease. We analyzed the cases with tsutsugamushi disease who lived in Chuncheon and neighboring communities to find out clinical manifestations, laboratory findings especially in severe cases. Methods : We reviewed the clinical records of the 81 cases who were diagnosed as tsutsugamushi in Chuncheon Sacred Heart Hospital from April, 1997 to December, 2002. We analyzed the clinical and laboratory findings of the 81 cases, and the treatment results of 79 cases who recieved the treatment in this hospital. We also compared the characteristics of the patients between admission to general ward and intensive care unit (ICU). Results : Seventy three cases developed the disease in autumn. Seventy cases had fever. 58 cases had eschar in their bodies. With the doxycycline treatment, forty five cases subsided the fever within 48 hours, but in 13 cases, fever continued after 5 days of treatment. Twenty five cases were admitted to ICU, 8 cases among them were treated with ventilator, 3 cases died due to the multi-organ failure. When we compared the clinical characteristics of the patients admitted to ICU with those to general wards, leukocyte count, the level of BUN and creatinine were higher, the albumin level was lower in ICU patients than those of general ward patients. Conclusion : Tsutsugamushi disease can progress to severe disease, and be fatal to the patients. So it is necessary to diagnose early and treat carefully. We suggest that leukocyte count, BUN, creatinine, or albumin can be used to predict the prognosis.(Korean J Med 69:190-196, 2005)

임펠러 슈라우드 면의 형상 변화에 따른 다익팬 성능

이호호(Ho Ho Lee),최항철(Hang Cheol Choi),이원석(Won Suk Lee),정재구(Jae Goo Jung),이윤표(Yoon Pyo Lee),김광호(Kwang Ho Kim),정진택(Jin Taek Chung) 한국유체기계학회 2010 유체기계 연구개발 발표회 논문집 Vol.2010 No.12