Draft genome sequence of Senegalimassilia sp. KGMB 04484 isolated from healthy Korean human feces

한국일,강세원,김지선,이근철,엄미경,서민국,김한솔,박승환,이주혁,박잠언,오병섭,유승엽,최승현,이동호,윤혁,김병용,이제희,이정숙,Han, Kook-Il,Kang, Se Won,Kim, Ji-Sun,Lee, Keun Chul,Eom, Mi Kyung,Suh, Min Kuk,Kim, Han Sol,Park, Seung-Hwan,Lee, Ju Huck,Park, The Microbiological Society of Korea 2019 미생물학회지 Vol.55 No.2

본 연구에서는 건강한 한국인 분변으로부터 Senegalimassilia sp. KGMB 04484 균주를 분리하였으며 PacBio Sequel 플랫폼을 이용하여 유전체서열을 분석하였다. 유전체는 G+C 구성비율이 61.18%이며, 2,300개의 유전자와 2,139개의 단백질 코딩 유전자, 21개의 rRNA 및 51개 tRNA로 구성되었으며, 염색체의 크기는 2,748,041 bp였다. 유전체의 주요 특징은 가수분해효소와 지방산생합성 및 대사에 관련된 유전자를 포함한다. 이러한 유전체의 분석은 KGMB 04484 균주가 사람의 건강 및 소화에 관여할 것으로 여겨진다. Senegalimassilia sp. KGMB 04484 was isolated from fecal samples obtained from a healthy Korean. The whole-genome sequence of Senegalimassilia sp. KGMB 04484 was analyzed using the PacBio Sequel platform. The genome comprises a 2,748,041 bp chromosome with a G+C content of 61.18%, 2,300 total genes, 2,139 protein-coding gene, 21 rRNA genes, and 51 tRNA genes. Also, we found that strain KGMB 04484 had some genes for hydrolysis enzyme, fatty acid biosynthesis and metabolism in its genome based on the result of genome analysis. Those genes of KGMB 04484 may be related to regulation of human health and digest.

기업규모별 위기관리 차이에 관한 탐색적 연구

홍한국(Han-Kook Hong),이봉구(Bong-koo Lee),임병학(Byung-hak Leem) 한국콘텐츠학회 2023 한국콘텐츠학회논문지 Vol.23 No.2

국내 기업의 대부분은 과거 위기에 직면한 경험이 있었음에도 불구하고 위기관리에 대한 인식이 부족하다. 이들은 위기를 단지 악재로만 간주하여 감추려고만 했을 뿐 위기를 사전에 예방하고 위기상황을 새로운 기회로 전환시키고자 하는 적극적인 대처는 부족한 것이 현실이다. 즉, 기업들은 잠재적 위기에 대한 사전계획이나 구체적인 대비책을 체계적으로 세우지 못했고, 큰 위험이나 사고를 겪은 후에야 비로소 위기관리에 대한 문제의식을 갖는 경향이 강했다. 이러한 위기에 대한 인식으로 인해서 위기관리에 관한 연구, 특히 기업규모별로 위기관리 관련 차이를 고찰한 연구는 미미한 실정이다. 이에 본 연구는 국내 201개의 기업을 대상으로 위기유형별 기업이 경험한 위기수준, 위기관리 중요성인식과 대응수준, 그리고 향후 중점 위기관리 대상에 대한 인식에 있어 기업규모별 차이를 고찰하였다. 본 연구는 기업이 적절한 위기대응 전략을 선택하고자 할 때 위기관리자가 위기를 효과적으로 관리하는 데 도움을 준다. Recently, most of domestic companies have struggled to deal with different kinds of crisis accrued from various sources. However, their perception on the crisis and crisis management system are extremely vulnerable. In particular, although almost all companies have had many business related crisis, they have tended to ignore the crisis with simply considering it as unlucky and not to approach it provocatively in preparing some preventive measures and turning it into an opportunity due to the lack of recognizing the importance of risk management. In other words, companies have not systematically prepared detailed plans for potential crisis, rather have tended to recognize the importance of having crisis management system after experiencing a detrimental crisis or accident. Due to the perception of these risks by companies, studies on risk management, especially those that examine the differences in risk management by the company size, are insignificant. The purpose of this study is to investigate the differences by the company size in terms of the degree of crisis experienced previously, the perception on the importance of crisis management system, the level of crisis management implementation, and the areas of crisis management being focused in the future for 201 domestic companies. This study helps crisis managers to manage crises effectively when the company want to select proper crisis response strategies.

흉부외상이 동반된 다발성 외상환자에서 폐손상 점수가 중환자실 치료에 미치는 영향

한국남 ( Kook Nam Han ),최석호 ( Seok Ho Choi ),김영철 ( Yeong Cheol Kim ),이경학 ( Kyoung Hak Lee ),이수언 ( Soo Eon Lee ),정기영 ( Ki Young Jeong ),서길준 ( Gil Joon Suh ) 대한외상학회 2011 大韓外傷學會誌 Vol.24 No.2

Purpose: Chest injuries in multiple trauma patients are major predisposing factor for increased length of stay in intensive care unit, prolonged mechanical ventilator, and respiratory complications such as pneumonia. The aim of this study is the evaluation of lung injury score as a risk factor for prolonged management in intensive care unit (ICU). Methods: Between June to August in 2011, 46 patients admitted to shock and trauma center in our hospital and 24 patients had associated chest damage without traumatic brain injury. Retrospectively, we calculated injury severity score (ISS), lung injury score, and the number of fractured ribs and performed nonparametric correlation analysis with length of stay in ICU and mechanical ventilator support. Results: Calculated lung injury score (<48 hours) was median 1(0-3) and ISS was median 30(8-38) in study population. They had median 2(0-14) fractured ribs. There were 2 bilateral fractures and 2 flail chest. Ventilator support was needed in 11(45.8%) of them for median 39 hours (6-166). The ISS of ventilator support group was median 34(24-34) and lung injury score was median 1.7(1.3-2.5). Tracheostomy was performed in one patient and it was only complicated case and ICU stay days was median 9(4-16). In correlation analysis, Lung injury score and ISS were significant with the length of stay in ICU but the number of fractured ribs and lung injury score were predicting factors for prolonged mechanical ventilator support. Conclusion: Lung injury score could be a possible prognostic factor for the prediction of increased length of stay in ICU and need for mechanical ventilator support.

Draft genome sequence of Olsenella sp. KGMB 04489 isolated from healthy Korean human feces

한국일,강세원,김지선,이근철,엄미경,서민국,박승환,이주혁,박잠언,오병섭,유승엽,최승현,이동호,윤혁,김병용,양승조,이정숙,Han, Kook-Il,Kang, Se Won,Kim, Ji-Sun,Lee, Keun Chul,Eom, Mi Kyung,Suh, Min Kuk,Park, Seung-Hwan,Lee, Ju Huck,Park, Jam-Eon,Oh, Byeon The Microbiological Society of Korea 2018 미생물학회지 Vol.54 No.4

Olsenella 속 균주들은 척추동물의 구강, 반추위 및 분변 등에서 분리된 것으로 알려져 있다. 본 연구에서는 건강한 한국인 분변으로부터 Olsenella sp. KGMB 04489 균주를 분리하였으며 PacBio Sequel 플랫폼을 이용하여 Olsenella sp. KGMB 04489 균주의 유전체서열을 분석하였다. 유전체는 G + C 구성 비율이 65.5%이고, 1,838개의 유전자와 rRNA 13개, tRNA 52개로 구성되었으며, 염색체의 크기는 2,108,034 bp였다. 또한, 유전체 분석 결과를 통해 가수분해효소와 항생제 합성 및 내성과 관련된 다양한 유전자를 발견하였다. The genus of Olsenella has been isolated from vertebrate animal mouth, rumen, and feces. Olsenella sp. KGMB 04489 was isolated from fecal samples obtained from a healthy Korean. The whole-genome sequence of Olsenella sp. KGMB 04489 was analyzed using the PacBio Sequel platform. The genome comprises a 2,108,034 bp chromosome with a G + C content of 65.50%, 1,838 total genes, 13 rRNA genes, and 52 tRNA genes. Also, we found that strain KGMB 04489 had some genes for hydrolysis enzymes, and antibiotic biosynthesis and resistance in its genome based on the result of genome analysis.

Draft genome sequence of Ruminococcus sp. KGMB03662 isolated from healthy Korean human feces

한국일,강세원,엄미경,김지선,이근철,서민국,김한솔,박승환,이주혁,박잠언,오병섭,유승우,유승엽,최승현,이동호,윤혁,김병용,이제희,이정숙,Han, Kook-Il,Kang, Se Won,Eom, Mi Kyung,Kim, Ji-Sun,Lee, Keun Chul,Suh, Min Kuk,Kim, Han Sol,Park, Seung-Hwan,Lee, Ju Huck,Pa The Microbiological Society of Korea 2019 미생물학회지 Vol.55 No.3

본 연구에서는 건강한 한국인 분변으로부터 Ruminococcus sp. KGMB03662 균주를 분리하고 유전체서열을 PacBio Sequel 플랫폼을 사용하여 분석하였다. 염색체의 크기는 2,707,502 bp로 G + C 구성 비율은 43.09%, 총 유전자수는 2,484개, 단백질 코딩 유전자는 2,367개, rRNA는 14개 및 tRNA는 53개로 구성되었다. 본 유전체로부터 가수분해효소, 지방산생합성 및 대사와 항생제생합성 및 내성 관련 유전자를 확인하였다. 이러한 유전체의 분석은 KGMB03662 균주가 사람의 건강 및 질병에 관여할 것으로 여겨진다. Ruminococcus sp. KGMB03662 was isolated from fecal samples obtained from a healthy Korean. The whole-genome sequence of Ruminococcus sp. KGMB03662 was analyzed using the PacBio Sequel platform. The genome comprises a 2,707,502 bp chromosome with a G + C content of 43.09%, 2,484 total genes, 2,367 protein-coding gene, 14 rRNA genes, and 53 tRNA genes. In the draft genome, genes involved in the hydrolysis enzyme, fatty acid biosynthesis, fatty acid metabolite, antibiotic biosynthesis, and antibiotic resistance have been identified. Those genes of KGMB03662 may be related to the regulation of human health and disease.

치아우식 원인균에 대한 한약재 추출물의 항균효과

이희원 ( Hee Woen Lee ),이푸른하늘 ( Pureunhaneul Lee ),권현정 ( Hyun Jung Kwon ),한국일 ( Kook Il Han ),한만덕 ( Man Deuk Han ) 한국치위생과학회 2013 치위생과학회지 Vol.13 No.1

Antimicrobial activity of extract from some traditional oriental medicinal plants were evaluated for their antimicrobial activity against six oral pathogens, Streptococcus salivarius, Streptococcus oralis, Streptococcus mutans, Streptococcus sanguinis, Lactobacillus acidophilus and Lactobacillus casei, which are associated with caries disease. The antimicrobial activity was examined by determining the inhibition zone using the disc diffusion assay. In antibacterial activity test, extracts of Scutellaria baicalensis, Chrysanthemum indicum, Kochia scoparia, Hydnocarpus anthelmintica and Caesalpinia sappan showed inhibitory effects (40 mg/ml) against tested caries bacteria. Especially, the C. sappan extract showed the strongest activity on S. oralis (40 mm), L. casei (35 mm) and S. mutans (28 mm). Thus, this result suggests that C. sappan may be applicable to preventing dental caries.

메조크기의 다공성 탄소에 담지된 고담지 합금 촉매에 대한 연구

봉성율(Bong, Sung-Yool),한국일(Han, Kook-Il),이재승(Lee, Jae-Seung),김인(Kim, In),김하석(Kim, Ha-Suck),김영애(Kim, Young-Ae),이윤우(Lee, Youn-Woo) 한국신재생에너지학회 2005 한국신재생에너지학회 학술대회논문집 Vol.2005 No.11

펄스 전기환원법을 이용한 고분자 전해질 연료전지용 저담지 전극의 제조

이재승(Lee, Jae-Seung),서정숙(Seo, Jung-Sook),한국일(Han, Kook-Il),김하석(Kim, Ha-Suck) 한국신재생에너지학회 2005 한국신재생에너지학회 학술대회논문집 Vol.2005 No.11

Streptococcus dysgalactiae로부터 분리된 히알루론산과 황화된 유도체의 구조와 항염증 활성

홍창일(Chang-Il Hong),정의길(Eui-Gil Jung),한국일(Kook-Il Han),김용현(Yong Hyun Kim),이성희(Sung Hee Lee),이홍섭(Hong Sub Lee),한만덕(Man-Deuk Han) 한국생명과학회 2016 생명과학회지 Vol.26 No.5

히알루론산(HA, Hyaluronic acid)은 β-1, 3-N-acetyl glucosamine과 β-1, 4-glucuronic acid가 반복된 선형 폴리머 고분자로서 생물학적 활성 및 생체친화성 특성 때문에 의약 및 약학분야에서 중요한 분자로 여겨지고 있다. 본 연구는 HA을 S. dysgalactiae으로 얻고, 화학적 방법을 통해 황화된 히알루론산(S-HA, Sulfated hyaluronic acid)유도체를 합성하여 그 구조와 항염증 활성을 비교하였다. HA의 생산은 S. dysgalactiae를 5 l 생물반응기를 이용하여 대량 배양하여 수용성 히알루론산(HA-WS, water soluble hyaluronic acid)과 비수용성 히알루론산(HA-WI, water insoluble hyaluronic acid)을 분리 정제하였다. 특히 HA-WI를 황화시켜 황화된 히알루론산(S-HA) 유도체를 합성하였으며, 그 수율은 90%로 나타났다. 합성된 S-HA의 구조를 FT-IR 및 ¹H/<SUP>13</SUP>C-NMR를 통해 S. dysgalactiae 로부터 생산된 표준 HA, HA-WS 및 HA-WI와 비교 분석한 결과, 황으로 치환된 양상을 확인하였다. 또한, S-HA의 항염증 활성을 RAW 264.7 대식세포를 통해 확인한 결과, S-HA는 천연 형태의 HA (HA, HA-WS)보다 nitric oxide (NO)와 COX-2 및 PGE₂ 유전자 발현이 유의하게 낮게 발현되었다. 염증 매개 cytokine인 TNF-α (<80 pg/ml) 및 IL-6 (<100 pg/ml)의 생성도 S-HA가 천연 HA보다 낮은 수준으로 정량되었다. 이 같은 결과에서 황화된 S-HA은 천연 히알루론산보다 용해성이 우수하고 염증관련 사이토카인의 생성 억제를 통해 항염증 효과를 나타내므로 염증치료제, 성형 및 생체 적용 약물전달 소재로 그 활용이 기대된다. Hyaluronic acid (HA) is an important macromolecule in medical and pharmaceutical fields. HA is a natural and linear polymer composed of repeating disaccharide units of β-1, 3-N-acetyl glucosamine and β-1, 4-glucuronic acid. This work aimed to confirm the structural characteristics and anti-inflammatory activities of HA and its chemically sulfated-HA. HA was produced from a fed-batch fermentation process using Streptococcus dysgalactiae in a 5 l bioreactor. HA was isolated water-soluble form (HA-WS) and water-insoluble form (HA-WI) from culture medium, and was obtained chemically sulfated-derivative (S-HA) that resulted in a 90% yield from HA-WI. The structural features of the sulfated- HA (S-HA) were investigated by FT-IR and ¹H-NMR spectroscopy. The FT-IR and NMR patterns revealed the similarity in both the FTIR spectrum as well as NMR spectrum of both reference standard and purified HA from S. dysgalactiae. The anti-inflammatory activities of HA and S-HA were examined on LPS-induced RAW 264.7 cells. S-HA was significantly inhibited production of pro-inflammatory mediators such as nitric oxide (NO) and PGE₂ and the gene levels of iNOS and COX-2, which are responsible for the production of NO and PGE₂, respectively. Furthermore, S-HA also suppressed the overproduction of pro-inflammatory cytokine TNF-α (<80 pg/ml) and IL-6 (<100 pg/ml) compared to that of HA-WI. The present study clearly demonstrates that HA-S exhibits anti-inflammatory activities in RAW 264.7 macrophage cells.

중형폐기물 소각시설의 수은, 납, 비소, 셀렌 배출특성

이한국,Lee Han-Kook 한국환경보건학회 2006 한국환경보건학회지 Vol.32 No.1

The aim of this study is to evaluate the emission characteristics of mercury, lead, arsenic, and selenium from medium size municipal solid waste incinerators(MSWIs) in Korea. The concentrations of mercury, lead, arsenic, and selenium emitted from medium size MSWI stack were $2.67\;{\mu}g/Sm^3,\;0.38\;mg/Sm^3,\;1.33\;{\mu}g/Sm^3,\;0.28\;{\mu}g/Sm^3$, respectively. The concentration levels of mercury, lead, arsenic in flue gas from medium size MSW incinerator stacks selected were nearly detected under the Korea criteria level. Removal efficiencies of mercury, lead, arsenic, and selenium in waste heat boiler(WHE) and cooling tower(CT) were $90.36\%,\;69.76\%,\;43.04\%,\;40.64\%$, respectively. In general, the removal efficiencies of mercury and lead in WHE were higher than those of arsenic and selenium in WHE. Emission gas temperature reduction from waste heat boiler(WHB) and cooling tower(CT) can control mercury and lead of medium size MSWIs. To evaluate the relationship between mercury, lead, arsenic, selenium of fly ash and those of flue gas, it was carried out to correlation analysis of each metal concentration in the fly ash and in the flue gas from medium size MSWIs. From the correlation analysis, the coefficients of mercury, lead, arsenic, and selenium were 0.61, -0.38, 0.87, 0.28, respectively. The results of correlation analysis revealed that it should be highly positive to the correlation coefficients of mercury and arsenic in the fly ash and those of the flue gas emitted from medium size MSWIs. As it were, the concentrations of mercury and arsenic of flue gas from medium size MSWIs are high unless mercury and arsenic in fly ash are properly controlled in dust collection step in medium size MSWIs. It was also concluded that mercury, lead, arsenic, and selenium from MSWIs stacks could be controlled by waste heat boiler(WHE) and dust collecting step in medium size MSWIs.