펩타이드H 에 의한 estrogen 의존성 유방암세포 증식억제

박잠언,김한복 호서대학교 기초과학연구소 2015 기초과학연구 논문집 Vol.23 No.1

청국장에서 분리하여 변형한 펩타이드 H (EVYYMY) Im M과 5pM daidzein 를 인간 유방암세포 MCF7 세포에 처리하면 세포증식은 억제 되었다. 또한 microarray data 분석을 통해 pp3rl (protein phosphatase 2B regulatory subunit1) 은 control에 비해 2.17배 증가하였고 ndrgl(n-myc downstream regulated 1 )은 1.85 배 감소했음을 확인했다 .

인간유방암 MDA-MB-231 세포에서 청국장추출물에 의한 TNFα 발현억제

박잠언,강충경,김한복,Park, Jameon,Kang, Choong Kyung,Kim, Han Bok 한국미생물학회 2016 미생물학회지 Vol.52 No.3

청국장은 대두발효식품으로 발효 중 생성된 다양한 펩타이드가 들어 있다. 이들 펩타이드가 포함된 청국장추출물을 세포에 처리하면 세포 신호전달에 영향을 미친다. 인간 유방암 MDA-MB-231 세포에 청국장추출물을 처리해 주었을 때 세포의 성장은 농도의존적으로 억제되었다. 청국장추출물이 유방암세포의 증식을 억제하였고 암과 염증이 관련이 있으므로, 청국장추출물이 염증 유전자의 하나인 $TNF{\alpha}$ 발현의 억제 여부를 알아 보았다. 인간유방암 MDA-MB 231 세포에 청국장 추출물을 처리하면 $TNF{\alpha}$ 발현은 억제되었다. $TNF{\alpha}$ 저해제는 자가면역질환 치료제로 개발되어 있다. 본 청국장추출물도 $TNF{\alpha}$ 억제 효과가 있으므로, 이들 자가면역 질환의 치료제로 개발될 수 있을 것이다. Chungkookjang, fermented soybeans, contains diverse peptides produced during fermentation. Fermented soybean extracts containing the peptides can affect cellular signal transduction. Proliferation of human breast cancer MDA-MB-231 cells were repressed dependent on concentrations of fermented soybean extracts. Since fermented soybean extracts inhibited breast cancer cell's growth, and inflammation is related to cancer, it is determined whether it can suppress inflammatory $TNF{\alpha}$ expression. $TNF{\alpha}$ expression in MDA-MB 231 cells treated with fermented soybean extracts was repressed by that extracts. $TNF{\alpha}$ inhibitors were developed as drugs for autoimmune diseases. Since fermented soybean extracts suppressed $TNF{\alpha}$ expression, it can be developed as those drugs.

Draft genome sequence of Blautia sp. KGMB01111 isolated from a healthy Korean human faeces

박잠언,김지선,최승현,한국일,강세원,이주혁,오병섭,유승엽,이근철,엄미경,서민국,김한솔,이동호,윤혁,김병용,이제희,이정숙,박승환 한국미생물학회 2020 미생물학회지 Vol.56 No.1

Blautia sp. KGMB01111 was isolated from fecal samples obtained from a healthy Korean. The whole-genome sequence of Blautia sp. KGMB01111 was analyzed using the PacBio Sequel platform. The genome comprises a 3,738,517 bp chromosome with G + C content of 44.6 mol%, 3,390 total genes, 3,154 protein-coding genes, 18 rRNA genes, and 63 tRNA genes. Blautia sp. KGMB01111 was confirmed to produce short chain fatty acid such as acetic acid by fermentation. We found that strain KGMB01111 had some genes for synthesis of acetic acid using galactose and amino acid in its genome based on the result of genome analysis. Those genes of KGMB01111 may be related to regulation of human health and digest by promoting gut epithelial lining.

Suppression of metastasis-related ERBB2 and PLAU expressions in human breast cancer MCF 7 cells by fermented soybean extract

박잠언,김한복,Park, Jameon,Kim, Han Bok The Microbiological Society of Korea 2018 미생물학회지 Vol.54 No.4

발효대두 청국장에는 대두단백질이 발효에 의해 분해 형성된 다양한 펩타이드류가 들어 있다. 청국장 추출물이 처리된 유방암세포의 microarray data와 잘 알려진 유방암 전이 마커를 합쳐서 새로운 연결망이 제조되었으며 이를 이용해 전이 마커와 발현 차이가 있는 단백질 사이의 상호작용을 체크하였다. 연결망 분석을 통해 PLAU (plasminogen activator, urokinase, uPA)와 ERBB2 (epidermal growth factor receptor 2)를 실제 전이 가능성을 보여주는 유전자로 선택하였다. MCF7 암세포를 청국장추출물로 처리하고 PLAU와 ERBB2 발현정도를 측정하였다. 청국장 추출물은 PLAU와 ERBB2 발현을 상당히 억제하였다. 청국장 추출물을 처리한 암세포에서 염증 마커인 NO의 생산이 감소하였다. 인간 유방암세포에서 PLAU와 ERBB2 발현을 특이적으로 감소시키는 펩타이드를 찾아내는 것은 흥미로운 일일 것이다. Chunkookjang, fermented soybean is rich in diverse oligopeptides which derived from cleavage of soybean proteins during fermentation. Microarray data containing differently expressed genes in breast cancer cells treated with fermented soybean extract and well known breast cancer metastasis markers were combined, and a new network was constructed. It is used to check interactions between the marker proteins and the differently expressed genes. Based on the network analysis, PLAU (plasminogen activator, urokinase, uPA) and ERBB2 (epidermal growth factor receptor 2) are chosen as possible metastasis genes. We treated breast cancer MCF7 cells with fermented soybean extract and measured expression levels of PLAU and ERBB2. Fermented soybean extract suppressed PLAU and ERBB2 expressions conspicuously. In the cancer cells treated with fermented soybean extracts, an inflammation marker, NO production was also reduced. It will be interesting to find specific peptides to suppress PLAU and ERBB2 expressions in human breast cancer cells.

인간유방암 MDA-MB-231세포에서 peptide H에 의한 TNFα 발현 억제

성대일,박잠언,강충경,김한복,Sung, Dae Il,Park, Jameon,Kang, Choong Kyung,Kim, Han Bok 한국미생물학회 2015 미생물학회지 Vol.51 No.3

청국장은 다양한 peptide류를 포함한다. 청국장 유래의 peptide H를 인간유방암 MDA-MB-231 세포에 처리했을 때, $TNF{\alpha}$ 발현은 뚜렷하게 억제되었다. $TNF{\alpha}$에 의해 유도되는 IL6 발현 역시, peptide H에 의해 감소될 수 있음을 시사해 준다. Peptide H구조는 glucocorticoid, dexamethasone과 전혀 유사하지 않아 그들과 다른 기작으로 $TNF{\alpha}$ 발현억제에 작용할 것을 시사해 준다. Peptide H는 $TNF{\alpha}$ 발현 억제 효과가 있으므로 보다 깊이 있는 연구를 바탕으로, 류마티스 관절염, 크론병 등의 치료제로 개발될 수 있기를 기대해 본다. Chungkookjang (fermented soybeans) contains diverse peptides. Human breast cancer MDA-MB-231 cells were treated with peptide H derived from Chungkookjang, and $TNF{\alpha}$ expression in the cells was conspicuously repressed, suggesting peptide H's regulation of IL6 since IL6 is induced by $TNF{\alpha}$. The structure of peptide H was different from those of glucocorticoid and dexamethasone, suggesting different mechanisms of $TNF{\alpha}$ expression suppression. Peptide H which reduces $TNF{\alpha}$ expression can be developed as drugs for cancer, rheumatoid arthritis and Crohn's disease, after more investigation.

한우 분변에서 분리된 Anaerosporobacter 속의 신종 균주 AGMB01083의 유전체 염기서열 초안

최승현,박잠언,최지영,강세원,이문수,이강현,이미경,이지영,이주혁,이정숙,박승환 한국미생물학회 2020 미생물학회지 Vol.56 No.2

The genus of Anaerosporobacter was isolated from a forest-soil sample. The Anaerosporobacter sp. nov. strain AGMB01083 was isolated from faeces of Korean cow. The strain describes a novel species according to whole-genome sequence of strain AGMB01083 using the Illumina platform. According to the 16S rRNA sequence similarity, the strain AGMB01083 was most closely related to Anaerosporobacter mobilis HY-37-4T (95.7%). The genome assembly consists of a 4,589,105 bp chromosome with a DNA G + C content of 36.2%, 3,981 total genes, 27 rRNA genes, and 70 tRNA genes.

인간 유방암 MDA-MB-231 세포에서 Peptide H에 의한 IL-6 발현 억제효과

성대일,박잠언,김한복,Sung, Dae Il,Park, Jameon,Kim, Han Bok 한국미생물학회 2014 미생물학회지 Vol.50 No.3

암, 류마티스, 크론병 등은 만성염증과 관련되어 있다. Interleukin-6 (IL-6)는 염증의 주요 매개인자이다. 청국장은 콩 발효식품으로 대두단백질이 분해되어 다양한 peptide가 생성되면서, 생리활성물질이 될 수 있다. 본 연구에서는 청국장에서 분리한 peptide (Gly-Val-Tyr-Tyr-Met-Tyr)를 가공한 6mer H, [(Glu-Val-Tyr-Tyr-Met-Tyr(EVYYMY)]가 유방암세포 MDA-MB-231에서 IL-6 발현을 억제할 수 있는지 여부를 결정하였다. MDA-MB-231 세포에 peptide H를 처리해 주면, IL-6 발현은 peptide를 처리하지 않은 control에 비해, 크게 억제되었으며, 세포의 성장은 농도의존적으로 억제되었다. 암 이외에, 류마티스, 크론병 등 만성염증 질환에서 IL-6 신호의 차단은 염증개선에 유효한 것으로 알려져 있다. Peptide H는 염증과 관련된 IL-6 발현의 감소효과가 있으므로, IL-6 관련 암, 류마티스, 크론병 등의 치료제 개발로 응용, 연결될 수 있을 것이다. Chronic inflammation is involved in cancers, rheumatoid arthritis, and Crohn's disease. Inerleukin-6 (IL-6) plays major roles in inflammation. Chungkookjang, fermented soybean contains diverse peptides produced by cleavage of soybean proteins. The peptides can be bioactive compounds. Peptide (Gly-Val-Tyr-Tyr-Met-Tyr was purified from Chungkookjang, and modified to be 6mer H, Glu-Val-Tyr-Tyr-Met-Tyr (EVYYMY). Peptide H's activity to suppress IL-6 expression in a human breast cancer cell, MDA-MB-231 was determined. IL-6 Expression was reduced in the cell treated with peptide H 25 times less than controls which were not treated with peptide H. Proliferation of MDA-MB-231 cells was inhibited by peptide H, which is concentration-dependent. Blocking of IL-6 signals is known to be effective in reducing inflammation in rheumatoid arthritis, Crohn's disease, and cancers. Since peptide H can reduce inflammatory IL-6 expression, application of this study will contribute to drug development for diseases which are caused by excessive IL-6.

Draft genome sequence of Anaerotignum faecicola KGMB03357 isolated from human faeces

최승현,김지선,박잠언,오병섭,유승우,유승엽,강세원,한국일,서민국,김한솔,이근철,엄미경,이동호,윤혁,김병용,이제희,이주혁,이정숙,박승환 한국미생물학회 2019 미생물학회지 Vol.55 No.4

The genus of Anaerotignum was isolated from a methanogenic reactor, black mud, anoxic digestor sludge, chicken caeca, and human faeces. Anaerotignum faecicola KGMB03357 was isolated from faeces of a healthy Korean. The whole-genome sequence of Anaerotignum faecicola KGMB03357 was analyzed using the PacBio Sequel platform. The genome consists of a 2,520,658 bp chromosome with a DNA G + C content of 47.3 mol%, 2,357 total genes, 28 rRNA genes, and 74 tRNA genes. Also, we found that strain KGMB03357 had various genes for biosynthesis of amino acid and antibiotic resistance in its genome based on the result of genome analysis.

Draft genome sequence of Bacteroides sp. KGMB 02408 isolated from a healthy Korean feces

유승엽,김지선,오병섭,유승우,박승환,강세원,박잠언,최승현,한국일,이근철,엄미경,서민국,김한솔,이동호,윤혁,김병용,이제희,이정숙,이주혁,Yu, Seung Yeob,Kim, Ji-Sun,Oh, Byeong Seob,Ryu, Seoung Woo,Park, Seung-Hwan,Kang, Se Won,Park, Jam-Eon,Choi, Seung-Hyeon,Han, The Microbiological Society of Korea 2019 미생물학회지 Vol.55 No.3

Bacteroides 속 균주들은 척추동물의 분변 등에서 분리된 것으로 알려져 있다. 본 연구에서는 건강한 한국인 분변으로부터 Bacteroides sp. KGMB 02408 균주를 분리하였으며 PacBio Sequel 플랫폼을 이용하여 Bacteroides sp. KGMB 02408 균주의 유전체서열을 분석하였다. 유전체는 G + C 구성 비율이 39.5%이고, 5,005개의 유전자와 rRNA 18개 tRNA 74개로 구성되었으며, 염색체의 크기는 5,771,427 bp였다. 또한, 유전체 분석 결과를 통해 산화-환원 효소와 메나퀴논 생합성 및 그와 관련된 다양한 유전자를 발견하였다. The genus of Bacteroides has been isolated from vertebrate animal feces. Bacteroides sp. KGMB 02408 was isolated from fecal samples obtained from a healthy Korean. The wholegenome sequence of Bacteroides sp. KGMB 02408 was analyzed using the PacBio Sequel platform. The genome comprises a 5,771,427 bp chromosome with a G + C content of 39.50%, 5,005 total genes, 18 rRNA genes, and 74 tRNA genes. Furthermore, we found that strain KGMB 02408 had some genes for oxidoreductases and menaquinone biosynthesis in its genome based on the result of genome analysis.

Complete genome sequence of Sutterella sp. KGMB03119 isolated from healthy Korean feces

유승우,오병섭,유승엽,박승환,강세원,김지선,한국일,이근철,엄미경,박잠언,최승현,서민국,김한솔,이동호,윤혁,김병용,이제희,이정숙,이주혁 한국미생물학회 2020 미생물학회지 Vol.56 No.1

The genus of Sutterella was isolated from human and dog feces, except Sutterella wadsworthensis was isolated from human appendiceal and peritoneal fluid. Sutterella sp. KGMB03119 was isolated from healthy human feces. The whole-genome sequence of the isolate was analyzed by PacBio Sequel platform. The genome consists a 2,987,884 bp chromosome with a G + C content of 58.30%, 2,436 total genes, 18 rRNA genes and 63 tRNA genes. In addition, we found that strain KGMB03119 had several genes for antibiotic resistance and bile acid resistance in its genome based on the result of genome analysis.