한국 동해 남부해역 대륙붕에 서식하는 대형저서동물군집 공간분포를 결정하는 환경요인

이정호,이정석,박영규,강성길,최태섭,김병모,류종성,Lee, Jung-Ho,Lee, Jung-Suk,Park, Young-Gyu,Kang, Seong-Gil,Choi, Tae Seob,Gim, Byeong-Mo,Ryu, Jongseong 한국해양학회 2014 바다 Vol.19 No.1

본 연구는 동해 남부 이산화탄소 해저지중저장 후보지 주변 수심 100-500 m 해역에서 저서생태계의 공간분포와 이를 결정하는 환경요인을 이해하기 위해 수행되었다. 2012년 8월-9월 총 16개 정점에서 대형저서동물군집과 환경요인을 조사하였다. 총 158종의 저서동물이 채집되었으며, 평균 서식밀도는 $843indiv/m^2$, 평균 생물량(습중량)은 $26.2g\;WW/m^2$로 나타났으며, 생물다양성이 높은 지역에서 주로 서식밀도가 높았다. 측정된 33개 환경변수를 대상으로 주성분분석을 실시한 결과 조사해역의 저서환경은 크게 퇴적물환경, 저층해수환경, 수심에 의해 지배된다. 저서동물의 분포는 세 가지 주성분과 밀접한 관련을 보였는데, 특히 사질함량이 많은 정점일수록 출현종수가 많아지고 생물량은 적어지는 경향이 관찰되었다. 조개 2종을 포함한 우점종 6종은 ${\Omega}$ aragonite, ${\Omega}$ calcite 농도가 높은 곳을 선호하였는데, 이 종들은 이산화탄소 유입으로 인해 해양산성화가 진행되면 가장 먼저 피해를 입을 수 있는 생물로 판단된다. 저서동물 상위 1% 우점종의 종조성을 바탕으로 집괴분석한 결과 지리적으로 뚜렷하게 구분되는 4개의 정점군이 분류되었다. 각 정점군은 고유의 저층해수 및 퇴적물 환경을 보이고 있으며, 대표적인 특성종으로는 A) Ampelisca miharaensis, (B) Edwardsioides japonica, (C) Maldane cristata, (D) Spiophanes kroeyeri을 들 수 있다. 측정이 용이한 5개의 환경요인(수심, 사질함량, 수온, 염분, pH)을 이용하여 4개의 정점군을 정확도 100%로 예측할 수 있는 판별함수모델을 제시하였다. 조사지역의 저서동물군집은 환경요인과 밀접한 관계를 보이고 있으며, 이는 환경변수에 기반하여 저서동물 분포를 예측할 수 있는 통계적모델 개발 가능성을 시사한다. This study aims to understand environmental factors that determine spatial distribution of macrozoobenthic community in the southern area (ca 100-500 m depth) of East Sea, Korea, known as a candidate site for carbon storage under the seabed. From sixteen locations sampled in the summer of 2012, a total of 158 species were identified, showing density of $843indiv/m^2$ and biomass of $26.2g\;WW/m^2$, with increasing faunal density towards biologically higher diverse locations. Principal component analysis showed that a total of 33 environmental parameters were reduced to three principal components (PC), indicating sediment, bottom water, and depth, respectively. As sand content was increasing, number of species increased but biomass decreased. Six dominant species including two bivalve species favored high concentrations of ${\Omega}$ aragonite and ${\Omega}$ calcite, indicating that the corresponding species can be severely damaged by ocean acidification or $CO_2$ effluent. Cluaster analysis based on more than 1% density dominant species classified the entire study area into four faunal assemblage (location groups), which were delineated by characteristic species, including (A) Ampelisca miharaensis, (B) Edwardsioides japonica, (C) Maldane cristata, (D) Spiophanes kroeyeri, and clearly separated in terms of geography, bottom water and sediment environment. Overall, a discriminant function model was developed to predict four faunal assemblages from five simply-measured environmental variables (depth, sand content in sediment, temperature, salinity and pH in bottom water) with 100% accuracy, implying that benthic faunal assemablages are closed linked to certain combinations of abiotic factors.

신부전증과 신병증이 동반된 렙토스피라증의 임상상

김성숙(Seong Suk Kim),빈기태(Ki Tae Bin),정민수(Min Soo Jeong),이강욱(Kang Wook Lee),김선영(Sun Young Kim),신영태(Young Tai Shin),이정호(Jeong Ho Lee) 대한내과학회 1995 대한내과학회지 Vol.48 No.5

N/A Objectives: Leptospirosis is known to be one of the most frequent zoonosis in Korea in addition to hemorrhagic fever with renal syndrome. The pathologic changes of leptospirosis are widespreaded and most prominent in lung, liver, skeletal muscle, adrenals, stomach, spleen, nervous ystem, and kidney. We evaluated the prevalence and clinical findings of leptospirosis associated renal insufficiency and nephropathy. Methods: This study was performed in 103 patients with leptospirosis admitted in Chungnam National University Hospital from Jan. 1985 to Dec. 1992. We classified three group, as uremic patients (serum creatinine21.4mg/dl) with abnormal urinary finding, non-uremic patients(serum creatinine< 1.4mg/dl) with abnormal urinary finding, and non uremic patients with normal urinary finding. Results: 1) Mean age was 49.2year old(17-75years). Ratio of man to woman was 1.5: l. 2) Eighty two patients(79.6%) showed abnormal urinary findings. Twenty patients(19.4%) showed elevated serum creatinine level above 1.4mg/dl, Sixty two patients(60.2%) showed abnormal urinalysis with normal renal function. 3) Fever and chills, maculopapular rash and myalgia were most frequent symptoms and signs. Dyspnea and confused mentality occrued more frequently in uremic patients than that in non-ruemic patients (p<0.01, p<0.05). 4) Platelet counts of uremic patients were significantly lower than those of patients who showed abnormal urinalysis findings with normal level of serum creatinine(p<0.01). 5) Hypoproteinemia was more prevalent in uremic patients than in non-uremic patients(p<0.05). Elevation of serum CPK and LDH level appeared more frequent in uremic patients than in non-uremic patients(p<0.05, p<0.05). 6) All patients with renal involvement recovered completely without renal replacement therapy. Conclusion: We thout that leptospirosis is a systemic infectious disease which shows renal insufficiency in one fifth of victims, and they manifest severe clinical features such as dyspnea or confused mentality. However the outcome of the patients with renal insufficiency is relatively good.

꽃게 ( Portunus trituberculatus ) Hemocyanin 의 정제 및 특성에 관하여

이강석,김상복,이정호 ( Kang Suk Lee,Sang Bog Kim,Jung Ho Lee ) 생화학분자생물학회 1985 BMB Reports Vol.18 No.2

The hemocyanin from the hemolymph of crab, Portunus trituberculatus which belong to crustacean was purified by a single step of ion-exchange column chromatography after dialysis and ultracentrifugation. The purified hemocyanin was revealed a high degree of polymorphism after analyzed it by Davis-polyacrylamide gel electrophoresis, the peroxidase activity, and copper staining method. On the other hand, the denatured hemocyanin was appeared to be homogeneous polypeptide by SDS-PAGE, and the approximate molecular weight was estimated to be 70,000. It was found that the purified hemocyanin was a glycoprotein in nature that contained 2 males of copper/l mole of hemocyanin, and it was estimated that their contents in normal body fluid were approximately 46%.

꽃게 (Portunus trituberculatus) Hemocyanin의 정제 및 특성에 관하여

이강석,김상복,이정호,Lee, Kang-Suk,Kim, Sang-Bog,Lee, Jung-Ho 생화학분자생물학회 1985 한국생화학회지 Vol.18 No.2

꽃게 (P. trituberculatus) hemolymph를 채취한 후 원심분리 및 DEAE-Sephadex A-50 column chromatgraphy를 행하여 hemocyanin을 정제하였다. 정제된 hemocyanin은 단일 polypeptide로 이루어져 있었으며 monomer의 분자량은 약 70,000으로 추정 되었다. 또한 꽃게 hemolymph 단백질중 hemocyanin의 함량은 46%, Cu는 단일 polypeptide 당 2 mole, 그리고 당 함량은 단백질 mg 당 $10\;{\mu}g$ 이었다. 한편 정제된 hemocyanin은 Ca등 2가 이온을 가하지 않고 pH 5.7~8.9인 상태에서 mono, di, tri, tetra, pentamer등 다양한 polymorphism을 나타내었다. The hemocyanin from the hemolymph of crab, Portunus trituberculatus which belong to crustacean was purified by a single step of ion-exchange column chromatography after dialysis and ultracentrifugation. The purified hemocyanin was revealed a high degree of polymorphism after analyzed it by Davis-polyacrylamide gel electrophoresis, the peroxidase activity, and copper staining method. On the other hand, the denatured hemocyanin was appeared to be homogeneous polypeptide by SDS-PAGE, and the approximate molecular weight was estimated to be 70,000. It was found that the purified hemocyanin was a glycoprotein in nature that contained 2 moles of copper/l mole of hemocyanin, and it was estimated that their contents in normal body fluid were approximately 46%.

Copper 촉매를 이용한 p-phenylenediamine (PPD) 합성에 관한 연구

김정석 ( Jung Suk Kim ),이상용 ( Sang-yong Lee ),이정호 ( Jung Ho Lee ),최원춘 ( Won Choon Choi ),강나영 ( Na Young Kang ),박선영 ( Sun Young Park ),김기웅 ( Ki Woong Kim ),임종성 ( Jong Sung Lim ),박용기 ( Yong-ki Park ),서휘민 ( 한국화학공학회 2016 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.54 No.3

액체 암모니아와 p-diiodobenzene (PDIB)을 반응물로, Cu계 화합물을 촉매로 사용하는Aromatic amination을 이용하여p-phenylenediamine (PPD)을 합성하였다. 촉매의 종류와 양, 환원제의 종류, 암모니아의 양, 반응 온도가 생성물의 분포에 미치는 영향을 조사하였다. Cu(I) 화합물과 Cu 분말은 촉매로서 작용한 반면 Cu(II) 화합물은 촉매로서 작용하지 않았다. 촉매의 양이 증가할수록 반응속도는 빨라지지만 부반응물인 aniline의 생성량도 증가하였다. Aniline 생성량은 또한 사용한 암모니아의 양이 증가할수록 감소하였다. 촉매 사용량을 줄이기 위해 환원제인 ascorbic acid, hydrazine, dihydroxyfumaric acid를 조촉매로 사용하면 반응속도가 크게 향상되었으나 부반응물인 aniline의 생성량 또한 증가하였다. 사용한 조촉매 중에서는 ascorbic acid와 dihydroxyfumaric acid를 사용하였을 경우가 hydrazine을 사용하였을 경우보다 반응속도가 빨랐고, dihydroxyfumaric acid를 사용하였을 경우 가장 적은 양의 aniline이 생성되었다. p-Phenylenediamine (PPD) was synthesized by aromatic amination of p-diiodobenzene (PDIB) using liquid ammonia and Cu-catalysts. The effects of the catalyst, reductant, ammonia quantity and reaction temperature on PPD production were investigated. Cu(I) compounds and Cu powder were selected as catalyst due to a higher selectivity than Cu(II) compounds. As the catalyst quantity increased, rate of PPD production as well as side reaction of aniline decreased with increasing the quantity of ammonia. Reductants such as ascorbic acid, hydrazine and dihydroxyfumaric acid were tested to lower the catalyst loading. The use of reductants resulted in increasing the reaction rate but also increased the amount of aniline The rate of reaction using ascorbic acid or dihydroxyfumaric acid was faster than that using hydrazine. The lowest side reaction of aniline was found in dihydroxyfumaric acid of reductants investigated.

Electrophoretic Heterogeneity of Portunus trituberculatus Hemocyanin Subunits

유병선,김상복,이강석,이정호,양규환,Yoo, Byung-Sun,Kim, Sang-Bog,Lee, Kang-Suk,Lee, Jeong-Ho,Yang, Kyu-Hwan 생화학분자생물학회 1989 한국생화학회지 Vol.22 No.1

본 연구에서는 꽃게 헤모시아닌 monomer의 전기영동적 이질성에 관해서 여러가지 전기영동 방법을 이용하여 연구하였다. 꽃게 헤모시아닌은 SDS를 포함한 전기영동에서 75,000 정도의 분자량을 갖는 단일 밴드를 나타냈다. 따라서 꽃게 헤모시아닌 구성체는 분자량 크기에 따른 이질성은 보이지 않았다. Discontinuous-polyacrylamide gel electrophoresis (PAGE) 방법에 의한 전기영동 결과 EDTA가 첨가되지 않은 5% 젤에서는 단일 monomer 밴드와 polymer 밴드들이 나타났다. 반면에 1 mM EDTA를 첨가한 discontinuous-PAGE에서는 monomer로 보이는 이중 밴드를 나타내어 꽃게 헤모시아닌이 이질적인 성분으로 구성되어 있을 가능성을 보였다. 한편 acrylamide 농도를 높게 하여 설시한 discontinuous-PAGE의 경우 네개의 monomer 밴드를 나타냈다. SDS-PAGE에서 분자량 차이에 의한 monomer의 이질성은 보이지 않았지만 조건을 달리한 discontinuous-PAGE에서 꽃게 헤모시아닌의 monomer가 최소한 네종류인 것이 밝혀졌다. 이러한 사실은 continuous-PAGE에서 확실히 나타났다. Dissociating buffer에서 실시한 continuous-PAGE에서도 네개의 monomer 밴드가 나타났는데 이를 각각 전기영동도에 따라 subunit I, II, III, IV라 명명하였다. Subunit heterogeneity of Portunus trituberculatus hemocyanin was investigated using various electrophoretic systems.SDS polyacrylamide gel electrophoresis (PAGE) was performed to investigate size homogeneity of hemocyanin. The electrophoretic pattern of purified hemocyanin with detergent, SDS in 7.5% gel revealed a single band with a molecular weight of about 75,000. The purified hemocyanin was then subjected to alkaline electrophoresis without detergent. Discontinuous-PAGE of hemocyanin in 5% gel showed single monomer band and higher polymeric bands. Discontinuous-PAGE containing 1 mM EDTA in gel and sample, however, showed two monomeric bands, indicating that the component of hemocyanin might be heterogeneous one. Discontinuous-PAGE in 7.5% acrylamide concentration showed at least four monomeric bands. Inorder to confirm the electrophoretic heterogeneity of Portunus trituberculatus hemocyanin subunits, continuous-PAGE was performed. Continuous-PAGE in a dissociating buffer(Tris/HCl-EDTA buffer pH 8.9)showed that monomeric subunits of Portunus trituberculatus hemocyanin were resolved into four distinct bands, designated as I, II, III, and IV, which agreed with the results of Discontinuous-PAGE in 7.5% gel.

꽃게 헤모시아닌 구성체의 전기영동적 이질성에 관한 연구

유병선,김상복,이강석,이정호,양규환 ( Byung Sun Yoo,Sang Bob Kim,Kang Suk Lee,Jeong Ho Lee,Kyu Hwan Yang ) 생화학분자생물학회 1989 BMB Reports Vol.22 No.1

Subunit heterogeneity of Portunus trituberculatus hemocyanin was investigated using various electrophoretic systems. SDS polyacrylamide gel electrophoresis (PAGE) was performed to investigate size homogeneity of hemocyanin. The electrophoretic pattern of purified hemocyanin with detergent, SDS in 7.5% gel revealed a single band with a molecular weight of about 75,000. The purified hemocyanin was then subjected to alkaline electrophoresis without detergent. Discontinuous-PAGE of hemocyanin in 5% gel showed single monomer band and higher polymeric bands. Discontinuous-PAGE containing 1 mM EDTA in gel and sample, however, showed two monomeric bands, indicating that the component of hemocyanin might be heterogeneous one. Discontinuous-PAGE in 7.5% acrylamide concentration showed at least four monomeric bands. In order to confirm the electrophoretic heterogeneity of Portunus trituberculatus hemocyanin subunits, continuous-PAGE was performed. Continuous-PAGE in a dissociating buffer (Tris/HCI-EDTA buffer pH 8.9) showed that monomeric subunits of Portunus trituberculatus hemocyanin were resolved into four distinct bands, designated as I, II, III, and IV, which agreed with the results of Discontinuous-PAGE in 7.5% gel.

광합성 세균 Rhodobacter capsulata 에 의한 우라늄 흡착

이강석,김진규,김인규,박효국,이정호 ( Kang Suk Lee,Jin Kyu Kim,In Gyu Kim,Hyo Kook Park,Jeong Ho Lee ) 한국하천호수학회 1990 생태와 환경 Vol.23 No.4

Aqueous waste effluents from nuclear fuel processing operations may contain trace quantities of heavy metals such as uranium, Conventional treatment of waste waters may be ineffective or expensive when uranium concentrations in the waste water must be reduced down to 1 ㎍/㎖ or less. The ability of some microorganisms to uptake or adsorb dissolved heavy metals offers an alternative method for waste treatment. The adsorption of uranium by Rhodobacter capsulata DSM 1710, a strain of photosynthetic bacteria, was examined to identify factors that might affect a process for the removal of uranium from aqueous solution. The optimal pH for uranium adsorption was 4.5. Temperature had little or no effect on the process since more than 95% of dissoved uranium was adsorbed to the bacterial biomass within 10 minutes after mixing of cell biomass at 25, 30, and 40 ℃, all the same. Maximum capacity of uranium adsorption by R. capsulata DSM 1710 was equivalent to 1/4 of the dry cell mass (248 ㎍ U/㎎-dry biomass), the highest among the reported so far.

IgA 신병증의 예후인자에 대한 연구

빈기태(Ki Tai Bin),나기량(Gi Ryang Na),오선미(Seoun Mee Oh),김종섭(Jong Sub Kim),김성숙(Seong Suk Kim),이강욱(Kang Wook Lee),신영태(Young Tai Shin),서광선(Kwang Sun Seo),이정호(Jeong Ho Lee) 대한내과학회 1996 대한내과학회지 Vol.51 No.6

N/A Objectives: IgA nephropathy is the commonest glomerulonephritis in the world. In contrast to initial report indicated a favorable prognosis, subsequent reports have shown a highly variable outcome leading to end-stage renal failure in significant proportion of patients. Because IgA nephropathy is a disease with variable rate of progression to chronic renal failure, identification of clinical and pathologic prognostic indicators for these patients is very important. Methods: To evaluate the clinical features of IgA nephropathy and clinicopathological parameters that are associated with deteriorated renal function, we analyzed retrospectively 149 patients who were diagnosed as IgA nephropathy at Chungnam National University Hospital from January, 1986 to June, 1994. We also evaluated the clinicopathological fin- dings of 23 patients who were followed for more than 36 months in order to determine the prognostic signigificance of various renal histopathological alterations and clinical parameters, Results: l) IgA nephropathy was disgnosed to 149 patients(26.5%) for same period among 565 biopsied patients. 2) It is most common in the 3rd and 4th decades. The mean age of the patients was 28.9 years, and male to female ratio ratio was 1: 1,25. 3) Fifty two percents of the patients presented with gross hematuria. Upper respiratory tract infection preceded grosss hematuria in 85% of the patients. 4) At the time of renal biopsy, 9,4% of the patients showed heavy proteinuria and nephrotic syndrome, 5) In Comparison of clinicopathological parameters between 14 patients with normal renal function and 9 patients with deteriorated renal function who had a follow-up period of more than 36 months, the results of the findings are as follows: 24 hours total urine protein more than 2.0 grams, elevated BUN and serum creatinine concentration and severe degree of renal histopathologic findings, such as mesangial proliferation and sclerosis, interstitial fibrosis and tubular atrophy showed poor prognostic results. Conclusion: Clinical features such as 24 hours total urine protein more than 2 grams, higher serum blood urea nitrogen and serum creatinine and severe degree of histopathologic features such as mesangial proliferation and sclerosis, interstitial fibrosis and tubular atrophy were associated with deteriorated renal function.

벼, 콩 및 채소류에 대한 Mn-54, Co-60, Zn-65, Cs-137의 토양-작물체간 전이계수

박찬걸,조용우,최용호,김국찬,이창우,이강석,이정호 대한방사선 방어학회 1991 방사선방어학회지 Vol.16 No.2

벼, 콩, 상치, 당근, 호박의 가식부위에 대하여 몇가지 토양에 있어서 토양-작물체간 Mn-54, Co-60, Zn-65, Cs-137의 전이계수를 포트 재배에 의한 방사성 추적자 흡수실험을 통하여 조사하였다. 핵종간 전이계수는 거의 모든 경우 Zn-65>Mn-54>Cs-137>Co-60의 순이었다. 콩이 벼보다 전반적으로 한 자리 정도 높은 값을 보였고 채소류의 경우에는 대체로 상치에서 가장 높고 호박에서 가장 낮은 값을 보였다. 강산성 토양에서는 약산성 토양에서보다 전이계수가 훨씬 높았다. 본 조사결과에 입각하여 한국인의 섭식경로 피폭선량 평가에 이용하기 위한 각 핵종의 전이계수치가 작물별로 제안되었다. Soil-to plant transfer coefficients of Mn-54, Co-60, Zn-65 and Cs-137 were estimated for the edible parts of the rice, soybean, lettuce, carrot, and squash grown in different soils by radiotracer uptake experiments using pot cultures. The transfer coefficients of radionuclides were n the order of Zn-65>Mn-54>Cs-137>Co-60 in most of the cases studied. The coefficients for soybean were roughly and order of magnitude higher than those for rice. Among vegetables, lettuce mostly showed the highest value and squash, the lowest. In the strongly acidic soils. From the data obtained, crop-specific transfer coefficients of the four nuclides were proposed for the use in Korean food-radiation dose assessment.