“한의 사제: `한의 소리`에 응답하신 서남동 목사”

서광선(Kwang Sun Seo),최영실(Young Sil Choi) 한국여신학자협의회 2016 한국여성신학 Vol.- No.83

방사선 조사된 고불포화지방산 식이를 섭취한 Mouse의 산화적 스트레스

서대영(Seo Dae Young),박선영(Park Sun Young),강명희(Kang Myoung Hee),서광선(Suh Kwang Sun),이선영(Ly Sun Yung) 韓國營養學會 2006 Journal of Nutrition and Health Vol.39 No.7

대두 함유 방사선 조사식이를 섭취한 Mouse의 산화적 스트레스

박선영(Park Sun Young),서대영(Seo Dae Young),서광선(Suh Kwang Sun),이선영(Ly Sun Yung) 韓國營養學會 2007 Journal of Nutrition and Health Vol.40 No.2

Food irradiation has been steadily increased in many countries concomitantly with increasing international trades. Harmful contaminants naturally occurred from foods which contain high levels of unsaturated fatty acids that are easily oxidized can affect the human anti-oxidation system through the generation of free radicals. Moreover, previous studies proved that r-irradiation may cause production of free radicals in food. We investigated the effect of r-irradiated soybeans in relation to oxidative stress in mice. Oxidative index of mice was evaluated by TBARS, DNA fragmentation in various organs such as blood lymphocytes, liver and kidney. Forty male ICR mice were equally divided into 4 groups and fed control diet or r-irradiated diet containing 50% soybeans (5, 10, and 20 kGy, respectively) for 8 weeks. Peroxide values of the irradiated diets were higher than that of the non-irradiated one and increased according to the storage period. There was no significant difference in weight gain as well as in TBARS value in plasma and kidney of all groups. Liver TBARS value of the group fed with irradiated diet at 20 kGy increased significantly compared with the control group (p<0.05). DNA oxidative damage as measured by alkaline comet assay showed that % tail DNA in the blood lymphocytes of 5 kGy and 10 kGy groups increased significantly over the control group (p<0.05). Also, tail moments of 5 kGy and 10 kGy groups were higher than that of the control group. Ultrastructural examination shows myeline figures and swollen mitochondria in parietal and intestinal epithelial cells of the group fed with irradiated diet. Therefore, considering unsaturated fatty acid content, consumption of soybeans r-irradiated with over 20 kGy or repeatedly may decrease the body's antioxidant mechanism. (Korean J Nutrition 40(2): 138~146, 2007)

증례 : 소화기 ; 급성 A형 간염에서 우연히 발견된 만성 B형 간염과 연관된 신조직 소견

정재진 ( Jae Jin Jeong ),김굉배 ( Goeng Bae Kim ),박설 ( Seol Park ),윤형식 ( Hyung Sik Yoon ),서광선 ( Kwang Sun Seo ),이계성 ( Gye Sung Lee ),강현모 ( Hyoun Mo Kang ) 대한내과학회 2011 대한내과학회지 Vol.80 No.1

저자들은 비전격성 형태로 발현된 급성 A형 간염으로 인한 급성 신부전을 경험하였다. 환자의 임상 경과와 병리 소견을 알기 위해 실시한 신조직 검사에서 급성 신부전의 특징적인 조직 소견인 급성 신세뇨관 괴사를 확인하였고, 광학 현미경 소견에서 IgA신병증 그리고 면역 형광 염색에서 과막증식 사구체 신염을 확인하여 역으로 만성 B형 간염으로 인한 신병증도 함께 진단할 수 있었다. 이후 실시한 혈액 투석으로 신기능이 회복된 예를 경험하였기에 이를 보고하는 바이며 이런 환자에서 특별한 금기가 없는 한 신조직 검사를 실시하는 것이 환자의 임상경과를 알고 치료하는데에 도움이 된다 하겠다. Hepatitis A is usually a self-limited liver disease that is treated conservatively. Acute renal injury complicating acute hepatitis A in the absence of fulminant hepatic failure is rare. We experienced a 33-year-old man who was healthy, and did not know that he was a hepatitis B virus (HBV)carrier with non-fulminant hepatitis A. He developed an acute kidney injury that necessitated dialysis therapy and a renal biopsy. He recovered within about 1 month. Pathologically, the renal biopsy showed acute tubular necrosis. It also showed IgA nephropathy and mesangium proliferation with glomerular nephritis, which are seen in chronic viral hepatitis B. We describe the association of acute hepatitis A with acute kidney injury and report the pathological findings of a renal biopsy related to chronic hepatitis B in an acute hepatitis A patient discovered by chance. (Korean J Med 2011;80:87-91)

성인 원발성 국소성 분절성 사구체경화증의 임상적 고찰

장윤경(Yoon Kyung Chang),최대은(Dae Eun Choi),이상주(Sang Ju Lee),박기현(Gi Hyun Park),양종오(Jong Oh Yang),나기량(Ki Ryang Na),서광선(Kwang Sun Seo),이강욱(Kang Wook Lee),신영태(Young Tai Shin) 대한신장학회 2002 Kidney Research and Clinical Practice Vol.21 No.4

목 적 : 원발성 국소성 분절성 사구체경화증(Focal and segmental glomerular sclerosis, FSGS)은 신사구체의 국소적인 분절성경화나 초자변성을 특징으로 하는 사구체 질환으로서, 성인 환자 중 50-70%가 신증후군으로 발현하며 신증후군의 경과와 예후는 미세변화형 신증후군에 비해 좋지 않고 신장이식 후에도 재발이 잦은 것으로 알려져 있다. 저자들은 원발성 FSGS의 임상적 특징과 신증후군 환자군의 치료 및 예후인자를 규명하고자 하였다. 방 법: 충남대학교병원에서 1989년 12월부터 2000년 1월까지 원발성 FSGS로 진단을 받은 환자 54명을 대상으로 후향적으로 임상상 및 신장조직검사결과를 분석하였다. 결 과: 추적관찰기간의 중앙값은 48.7(6-123)개월이었고, 평균 연령은 36.8±14.6(15-75)세, 전체 남녀비는 1.08 : 1이었고 30세 이하에서는 2.8 : 1이었다. 원발성 FSGS 신증후군의 남녀비는 1.4 : 1이었다. 진단당시 혈뇨를 보인 예는 24명(44%), 고혈압이 동반된 예는 21명(39%), 고질소혈증을 보인 예는 8명(15%)이었고, 전체 54명 중 31명(57%)이 신증후군 환자였다. 신장조직의 소견에서 간질의 섬유화는 평균 16.89±16.2(3-70)%이었고 신증후군과 비신증후군 환자군간에 간질의 섬유화정도에 유의한 차이는 없었다. 신증후군과 비신증후군 환자군간에 만성신부전으로의 진행정도도 유의한 차이가 없었다. 신증후군을 보인 환자군에서 첫 치료에 대한 반응은 완전관해가 16명(52%), 부분관해가 7명(22%), 무반응이 8명(26%)이었고, 관해군 23명 중 18명 (78%)이 재발하였다. 관해군(23명)과 무반응군(8명)의 비교에서 무반응군이 진단시 신기능이 좋지 않았으며, 사구체의 구상경화가 더욱 심했고, 심한 간질의 섬유화를 보였다. 추적조사에서 관해군과 무반응군에서 만성신부전으로 진행한 예는 각각 2명(9%), 6명(75%)으로 유의하게 무반응군이 만성신부전으로의 진행이 많았다. 결 론 : 원발성 FSGS에 의한 신증후군 환자에서 진단당시 고질소혈증, 사구체 여과율의 감소, 사구체 구상 경화의 증가, 간질 섬유화가 증가된 환자들은 불량한 예후를 보였으며, 특히 첫 스테로이드치료에 대해 무반응을 보인 경우도 좋지 않은 예후를 보였다. N/A

IgA 신병증의 예후인자에 대한 연구

빈기태(Ki Tai Bin),나기량(Gi Ryang Na),오선미(Seoun Mee Oh),김종섭(Jong Sub Kim),김성숙(Seong Suk Kim),이강욱(Kang Wook Lee),신영태(Young Tai Shin),서광선(Kwang Sun Seo),이정호(Jeong Ho Lee) 대한내과학회 1996 대한내과학회지 Vol.51 No.6

N/A Objectives: IgA nephropathy is the commonest glomerulonephritis in the world. In contrast to initial report indicated a favorable prognosis, subsequent reports have shown a highly variable outcome leading to end-stage renal failure in significant proportion of patients. Because IgA nephropathy is a disease with variable rate of progression to chronic renal failure, identification of clinical and pathologic prognostic indicators for these patients is very important. Methods: To evaluate the clinical features of IgA nephropathy and clinicopathological parameters that are associated with deteriorated renal function, we analyzed retrospectively 149 patients who were diagnosed as IgA nephropathy at Chungnam National University Hospital from January, 1986 to June, 1994. We also evaluated the clinicopathological fin- dings of 23 patients who were followed for more than 36 months in order to determine the prognostic signigificance of various renal histopathological alterations and clinical parameters, Results: l) IgA nephropathy was disgnosed to 149 patients(26.5%) for same period among 565 biopsied patients. 2) It is most common in the 3rd and 4th decades. The mean age of the patients was 28.9 years, and male to female ratio ratio was 1: 1,25. 3) Fifty two percents of the patients presented with gross hematuria. Upper respiratory tract infection preceded grosss hematuria in 85% of the patients. 4) At the time of renal biopsy, 9,4% of the patients showed heavy proteinuria and nephrotic syndrome, 5) In Comparison of clinicopathological parameters between 14 patients with normal renal function and 9 patients with deteriorated renal function who had a follow-up period of more than 36 months, the results of the findings are as follows: 24 hours total urine protein more than 2.0 grams, elevated BUN and serum creatinine concentration and severe degree of renal histopathologic findings, such as mesangial proliferation and sclerosis, interstitial fibrosis and tubular atrophy showed poor prognostic results. Conclusion: Clinical features such as 24 hours total urine protein more than 2 grams, higher serum blood urea nitrogen and serum creatinine and severe degree of histopathologic features such as mesangial proliferation and sclerosis, interstitial fibrosis and tubular atrophy were associated with deteriorated renal function.

특발성 및 이차성 막성 신병증의 임상적 및 병리학적 고찰

서광선 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.2

A clinicopathological analysis for 83 cases of membranous nephropathy(MN) has been carried out from a series of renal biopsy specimens which were studied at Department of Pathology, College of Medicine, Chungnam National University, during a period of 8 years from October 1983 to September 1991. The following results were obtained : 50 cases(60.2%) were classified as an idiopathic MN and 33 cases(.39.8%) had an associated condition, such as hepatitis B virus or hepatitis C virus infection 18 cases(54.5%), systemic lupus erythematosus 9 cases(27.3%), pulmonary tuberculosis 3 cases(9.1%), syphilis 2 cases(6.1%), and diabetes mellitus 1 case (3.0%). The latter group was considered as a secondary MN. Although secondary MN is generally indistinguishable in all categories from idiopathic MN, on comparison of histopathologic features, it can be concluded that the following findings such as mesangial proliferation, mesangial expansion, deposits in multiple(mesangial and subendothelial) sites, and predominant IgA deposits are characteristic of secondary MN.

자발적 초점성분절성사구체경화증 Mouse 모델에서 TGF-β및 MCP-1 유전자발현에 미치는 Renin Angiotensin System 의 영향에 관한 연구

신영태,서광선,이강욱 충남대학교 의과대학 지역사회의학연구소 1999 충남의대잡지 Vol.26 No.1

The role of angiotensin Ⅱ in the pathogenesis of the progressive renal injury process has been studied in many human renal diseases and animal renal injury models. The inhibition of angiotensin Ⅱ also has been reported to be effective to attenuate renal injury process in many human renal diseases and animal models. The activation of TGF-β and MCP-1 gene is thought to be related with renal injury process. A mutant strain FGS/Nga/Kist mouse is reported to develop focal and segmental glomerulosis, spontaneously. In order to evaluate renal pathology, MCP-1 and TGF-β gene expressions and the effect of renin-angiotensin system inhibition, 8 FGS/Nga/Kist mice were treated by angiotensin converting enzyme inhibitor, enalapril (150 mg/L in drinking water) for 12 weeks. Another non-treated, agematched 8 FGS/Nga/Kist mice and 6 nomal CBA/Nga mice were also included in this study. The urinary protein excrtion, magnitude of glomerular sclerosis, and renal express of MCP-1 and TGF-β were evaluated by uristick, light microscopic examination and competitive RT-PCR, respectively. CBA/Nga mice did not show significant proteinuria. However, urinary protein scores of enalapril-treated FgS/Nga/Kist mice was significantly lower than that of non-treated FGS/Nga/Kist mice (2.0±0.3, 4.1±0.5, respectively, p <0.05). Focal and segmental glomerular scierosis index of enalapril treated FGS/Nga/Kist mice was significantly lower than that of non-treated FGS/Nga/Kist mice (120±13, 189±15, respectively, p < 0.05). The level of renal TGF-β gene expression of enalapril treated FGS/Nga/Kist was significantly higher than that of CBA/Nga mice (4.7±1.3, 1.0±0.3, respentively, p< 0.05). But, enalapril treated FGs/Nga/Kist mice showed significantly lower level of TGF-β expression than that of non-treated FGS/Nga/Kist mice(p<0.05). MCP-1 gene expression of untreated FGS/Nga/Kist mice was significantly lower than that of CBA/Nga mice. However, enalapril treated FGS/Nga/Kist mice showed markedly increased level of MCP-1 gene expression compared to the untreated FGS/Nga/Kist mice(9.8±1.8, 3.6±1.2, respectively, p<0.05). With the above result, we speculate that angiotensin converting enzyme ingibition attenuates the renal injury process in this model and the regulation pathway of MCP-1 by renin-angiotensin system may be different from other animal renal injury models.

일측 요관폐쇄에 의한 실험적 수신증에서 신조직내 MCP-1 및 TNF-α유전자 발현

나가량,황평주,김종학,구영선,강민규,서광선,이강욱,신영태 충남대학교 의과대학 지역사회의학연구소 1999 충남의대잡지 Vol.26 No.2

Early cellular and molecular derangements have been suggested as potential pivotal factors for the development of renal injury, such as interstitial fibrosis after the experimental hydronephrosis. Several recent studies have been demonstrating that one of the initial events taking place in the progressive renal injury process is the mononuclear cell infiltration in the glomeruli and tubulointerstitiuim. In order to evaluate the renal light microscopic findings as well as the renal cortical MCP-1 and TNF-α gene expressions which are modulating inflammatory process and the recruitment of mononuclear cells in many experimental models of renal injury, Sprague-Dawley rats underwent unilateral ureteral obstruction (UUO) and sham operation under the thiopental sodium anesthesia(50 mg/kg intraperitoneal injection). Three and 7 days after surgery, rats were sacrificed. By the competitive RT-PCR, the levels of TNF-α and MCP-1 mRNA in renal cortical tissues were measured. The magnitude of mononuclear cell infiltration and interstitial widening were evaluated by standard point counting method. The level of MCP-1 gene expression was significantly increased in UUO group compared to sham group at 3 and 7 days after surgery(UUO 3 days 8.99 ± 0.45, UUO 7 days 11.2 ± 0.26, sham 3 days 1.00 ± 0.08, sham 7 days 0.94 ± 0.05, p < 0.01, p < 0.01, mean ± SEM, respectively). The level of MCP-1 mRNA at 7 days after surgery was significantly higher than that at 3 days in UUO group. The TNF-α gene expression level of UUO group was also significantly higher than that of sham group at 3 and 7 days after surgery(UUO 3 days 1.73 ± 0.16, UUO 7 days 2.03 ± 0.21, sham 3 days : 1.00 ± 0.06, sham 7 days 1.09 ± 0.03, p < 0.01, p < 0.01, respectively). But there was no significant difference between the levels at 3 and 7 days after surgery in UUO group(p>0.05). On the light microscopic examination, the relative volume of interstitium (RVI) and the total count of infiltrated mononuclear cells(MCC) at 3 and 7 days after surgery in UUO group were also significantly higher than those of sham group(RVI ; UUO 7 days 14.9±1.0%,sham 6.0±0.7%, MCC; UUO 7days 34.0± 1.6, sham 3.7± 0.6, p < 0.01, p < 0.01, respectively). In conclusion, we speculated that the early up-regulation of MCP-1 and TNF-α genes of renal cortical tissue in this experimental hydronephrosis model is closely related to the mononuclear cell infiltration and tubulointerstitial fibrosis.