Topical application of epidermal growth factor accelerates wound healing by myofibroblast proliferation and collagen synthesis in rat

이장헌,Young-Bae Kwon,Hyun-Woo Kim,노대현,윤서연,Rong-Min Baek,Jeum -Yong Kim,권해용,이광길,박영환 대한수의학회 2006 Journal of Veterinary Science Vol.7 No.2

미성숙 쥐에 이식한 뇌하수체의 경시별제거가 배란유도와 Estrogen 및 Progesterone수준에 미치는 영향

이장헌,김영훈,이완 韓國受精卵移植學會 1987 한국동물생명공학회지 Vol.2 No.1

The present work was designed to understand the mechanism of superovuiation and the cause of early embryo loss and Implantation failure in the superovulating immature female rats which were elaborated by a pituitary gland transplantation. A pituitary gland obtained from the orchidectomized rats was transplanted under the right kidney capsule of 28 day old female rats (PGT group) on the starting day of experiment which was designated as Day 2. The grafted pituitary glands were removed at 6h (RPGT 6h group), 12h (RPGT 12hgroup) and 24h (RPGT 24h group) after the transplantation. Control rats were treated with 41U PMSG on Day 2 (PMSG group). The estrous cycle and the levels of plasma progesterone and estradiol-17 were observed on Day 0, Day 5, respectively. The implantation sites, the weights of ovary and uterus, and the number of corpora lutea were examined in all group on Day 8. The resuft obtained were summarized as follows: 1. The percentages of the number of the rats in proestrus and estrus were 93.0%, 82.6%, 0%, 90.7% and 89.5% in PMSG, PGT, RPGT6h, RPGT12h and RPGT24h group, respectively. The synchronization of estrus cycle was dchieved in all groups. 2. The mating rates of each group were 80.2, 75.0, 0, 56.4, 57.8% in PMSG, PGT, RPGT6h, RPGT12h, RPGT24h group, respectively. 3. The numbers of copora lutea on Day 8 were 47.1 i 4.9, 18.1 0.5, 14.1 i 0.3 and 8.9 0.3 in PGT, RPGT24h, RPGTl2h and PMSG group, respectively. There were signIficantly difference between all groups (P<0.05). 4. The numbers of implantation sites (18.1 +- 4.0) in PGT group on Day 8 were higher than those of PMSG (8.5 2.5), RPGT 12h (9.8 i 0.2) and RPGT 24h group (10.8 i 0.2) (P<0.05). 5. The ovarlan weights in PGT (95.2 14.3mgIlOOg BW), RPGT 12h (51.7 0.6mgIlOOg BW), and RPGT24h (57.9 0.9mg/l00g 8W) groups were significantly higher than those of PMSG group (30.4 7.4mg/l00g BW) (P<0.05). 6. The uterine weights in PMSG (672.4 4.7mg/l00g 8W), and PGT (660.7 7.8mg/l00g BW) groupswere greater than those of RPGT 12h (403.0 1.lmg/lOOg BW) and RPGT 24h (490.1 0.9mg/l00g BW) group (P<0.05). 7. The plasma progesterone levels in PGT groups (15 lng/ml) on Day 5 were higher than those of PMSG (83ng/ml), RPGT 12h (S7ng/ml), RPGT24h (8lng/ml) group (P<0.05). 8. The plasma estradiol-17 levels in PMSG group (lBSpg/ml) on Day S were higher than those of RPGT 24h (l3pg/ml) group (P<0.05). But estradiol-17 levels in PGT and RPGT 12h group were too low to discuss.

Synthetic peptide를 이용한 mu-opioid receptor에 대한 항혈청의 생산과 검정

이장헌,권영배,한호재,Lee, Jang-hern,Kwon, Young-bae,Han, Ho-jae 대한수의학회 1999 大韓獸醫學會誌 Vol.39 No.1

In the present study we have analyzed the characteristics and distribution of the mu-opioid receptor(MOR) by raising anti-peptide antisera to the C-terminal peptide of MOR. The antisera against MOR was produced in New Zealand White rabbit against 15 residue corresponding to amino acids, 384-398 of the cloned rat MOR. The antigenic peptide was synthesized using an Applied Biosystems 432 solid-phase peptide synthesizer. The specificity and identification of the antisera were tested by analysis of transfected cells, epitope mapping and immunohistochemical method. COS-7 cells electroporated with MOR cDNA were used to evaluate the characteristics and subcellular distribution of MOR. MOR immunoreactivity was prodominent in the plasmalemma and subcellular compartments such as endoplasmic reticulum, Golgi apparatus and vesicle like structure. Furthermore, both tissue sections and transfected cell lines could be immunostained with these antisera and the immunoreactivity was abolished when anti-MOR sera were preincubated with the peptide against which they were raised. Based on epitope mapping analysis, all antisera appeared to have a similar epitope, which was determined to be within the last amino acid, 391-398. Moreover, immunohistochemistry showed that MOR immunoreactivity was observed in many brain areas including cerebral cortex, striatum, hippocampus, locus coeruleus and the superficial laminae of the dorsal horn. These stained spinal cord and brain areas showed the mirrored pattern observed in auto radiographic studies of mu-opioid binding as well as a pattern similar to that seen by is situ hybridization for MOR. Thus, several lines of evidence support the conclusion that the antisera produced in the present study most likely recognize mu-opioid receptor. These results suggest that MOR antisera may be utilized as useful tool to analyze the physiological and pharmacological studies for mu-opioid receptor in the future.

Morphine을 전신투여한 랫드의 뇌에서 분비되는 amino acid 성 신경전달물질 측정을 위한 미세투석법의 개선에 관한 연구

이장헌,Lee, Jang-hern,Beitz, Alvin J 대한수의학회 1998 大韓獸醫學會誌 Vol.38 No.3

In the present study, we designed and constructed new microdialysis probe in order to improve the efficacy and accuracy of microdialysis method. In addition, extracellular concentrations of GABA, glutamate, aspartate and glycine were monitored with new designed probe in the lateral portion of the ventrocaudal periaqueductal gray using unanesthetized and unrestrained rats. Furthermore, the effect of opiates on release of these amino acids, especially GABA, was analyzed by measuring their concentration in PAG dialysates following veratridine administration in the presence of systemic morphine. The results were summerized as follow : 1. The damaging rates of 1.0mm or 1.5mm window probe were 12.5% or 42.8%, respectively. In the group using 1.5mm window probe, the damaging area was extended into mesencephalic aqueduct because of microdialyzing pressure. 2. Because of the unique design of our probes with an opening facing one side, dialysis occurs in a hemisphere($600{\mu}m$ in mediolateral direction and $100{\mu}m$ in opposite side of the dialysis probe) around the opening rather than in a spherical shaped configuration which is typical of most commercially available probe designs. 3. Glutamate, taurine and glycine were present in the highest concentration in the dialysate sample obtained before treatment with veratridine, whereas, aspartate and GABA were present in the lowest concentration. 4. The concentration of all 5 amino acids increased significantly following $75{\mu}m$ veratridine perfusion into lateral ventrocaudal PAG. 5. There was no significant difference between basal and peak amino acid concentrations according to window sizes. 6. Morphine had no effect on baseline concentrations of amino acids in dialysates obtained from the lateral PAG as compared to saline treated controls. However, following veratridine treatment, morphine selectively affected GABA release in the lateral ventrocaudal PAG as compared to saline treated controls. These results suggest that GABAergic interneurons in the PAG are inhibited by opioids. Therefore, endogenous enkephalins or endorphins may directly inhibit intrinsic GABAergic intemeurons and block their tonic inhibition of PAG-NMR projection neurons. Moreover, new designed probes demonstrate improved efficiency and accuracy in collecting samples as compared to commercial types of microdialysis probes.

2007 한국건축문화대상 준공건축물부분 우수상-중앙하이츠

이장헌,Lee, Jang-Heon 대한건축사협회 2008 建築士 Vol.2008 No.1

Chronic cannula implantation 및 microdialysis가 periaqueductal gray내 신경세포 활성에 미치는 영향

이장헌,한호재,양일석,Lee, Jang-hern,Han, Ho-jae,Yang, Il-suk 대한수의학회 1998 大韓獸醫學會誌 Vol.38 No.4

Immunohistochemical technique of the c-fos primary gene protein, Fos, was used to analyze the effects of external factors on the neuronal activities in the periaqueductal gray(PAG) of the intact rats, sham-operated rats and post-operated stress control rats. In addition, the number of Fos positive neurons has been evaluated to verify the effects of cannula implantation and veratridine treatment on the neuronal activities in PAG area. The results were summerized as follow : 1. There was no significant difference in the number of Fos positive neurons observed in the caudal and middle portion of lateroventral PAG from cannula implanted rats and sham operated rats. 2. The number of Fos positive neurons in the PAG was not changed by the stress induced by connection of collecting tube to rats for 12 hours as compared to that of intact rats. 3. In the saline treated group, the Fos immunoreactivity in the PAG did not changed at 30 minutes and 1 hour after saline treatment as compared to that of intact rats. However, the number of Fos positive neurons was significantly increased at 2 hours after treatment compared to that of saline treated rats at 30 minutes after treatment. 4. The Fos immunoreactivity was dramatically increased at 30 minutes, 1 hour and 2 hours after veratridine treatment as compared to those of saline treated groups. The number of Fos immunoreative neurons showed the maximal level at 2 hours after veratridine treatment. 5. The Fos positive neurons induced by saline and veratridine treatment were mainly distributed in front of the microdialysis window. These results suggest that new microdialysis demonstrated in this study improves efficiency and accuracy to confine the neuronal activity in front of microdialysis window site. Moreover, this directional specificity allows us to locate probe tips adjacent to the brain area of the interest site rather than centering the probes within that brain area. Finally, This microdialysis method can be used to dialyse the neurotransmitters using concious and freely moving rats.

전기사용합리화의 실태와 문제점 및 대책

이장헌 대한전기협회 1989 대한전기협회지 Vol.- No.147

Etorphine을 만성투여한 쥐에서 나타나는 μ-opioid 수용체의 숫적 감소에 관한 면역조직화학법적 연구

이장헌(Jang-Hern Lee),Robert Elde(Robert Elde) 한국독성학회 1999 Toxicological Research Vol.15 No.3

A compensatory decrease in the number of active receptor is one possible mechanism for the development of drug tolerance. This agonist induced down-regulation has been reported in several hormonal or neurotransmitter systems. However, there was a lack of correlation between the time course of receptor down-regulation and the loss of pharmacological effects of the drug. In this study, we utilized immunohistochemical technique to investigate the modulatory effect of chronic etorphine treatment to mu opioid receptor levels. Male Sprague-Dawley rat was rendered tolerant to etorphine by s.c. implantation of osmotic minipumps containing 3 mg/ml of etorphine HCl for 1 or 5 days. During this period, there was a time-dependent increase in the AD50 values of etorphine to inhibit the tail-flick response and an increase in naloxone-precipitated withdrawal signs. Rat brains were removed, frozen, coronally sectioned (14 ㎛) and processed for mu opioid receptor immunohistochemistry by the avidin-biotin complex (ABC) method. Significant decreases in mu opioid receptor immunodensity were observed in many brain regions such as cauputamen (CPu) , thalamic nucleus (TN), raphe nucleus (RN) and amygdalohippocampal area (Amy). Time dependent decreases in mu opioid receptor immunoreactivity were detected and reached a plateau around 5 days after chronic treatment of etorphine. No significant change in immunoreactivity of leuenkephalin after chronic treatment with etorphine was found. Our conclusion is that chronic treatment with etorphine of rats down regulates mu opioid receptors in the brain mediated by cellular internalization of receptor. This may be an important mechanism for etorphine tolerance.

신경회로망을 이용한 비정상적인 패킷탐지

이장헌(Jang-hun Lee),김성옥(Sung-Ok Kim) 한국정보보호학회 2001 정보보호학회논문지 Vol.11 No.5

Bee Venom Acupoint Stimulation Increases Fos Expression in Catecholaminergic Neurons in the Rat Brain

권영배,이장헌,한호재,Alvin J Beitz 한국분자세포생물학회 2004 Molecules and cells Vol.17 No.2

Fos immunocytochemistry was combined with tyrosine hydroxylase (TH) or dopamine-beta-hydroxylase (DBH) immunolabeling to examine brainstem catecholaminer-gic neuronal activation resulting from bee venom (BV) stimulation of the Zusanli acupoint (ST36) in Sprague-Dawley rats. BV injection into the Zusanli acupoint caused increased Fos expression in catecholaminergic neurons located in the hypothalamic arcuate nucleus (Arc), the dorsal raphe (DR), the A5 cell group (A5) and the locus coeruleus (LC). BV acupoint stimulation significantly increased Fos-TH double-labeled neurons in the Arc, LC and DR. Fos-DBH positive neurons were also increased by BV acupoint stimulation in the LC and A5. In contrast BV stimulation of a non-acupoint only increased Fos expression and Fos-TH double-labeled neurons in the Arc. These data indicate that BV acupoint stimulation activates brainstem cate-cholaminergic neurons and that this activation under-lies BV acupoint-induced antinociception.