생쥐 간의 Triacylglycerol 생합성에 있어서 Palmitoylcarnitine의 지방산 공여체로서의 역할

이윤경,정진성,조기승,Lee, Youn-Kyung,Chung, Jin-Sung,Cho, Key-Seung 생화학분자생물학회 1986 한국생화학회지 Vol.19 No.3

$^{14}C$-Palmitoylcarnitine을 생쥐 간 균질물과 반응시킨 결과 1시간 후 33%, 3시간 후에 51%, 6시간 후에 최고 61%의 가수분해를 나타냄으로서, 동물체의 간이 acylcarnitine의 중요한 대사부위 임을 나타내 주었다. $^{14}C$-Palmitoylcarnitine ($^{14}C$-pal. carn)에 의한 $^{14}C$-triacylglycerol ($^{14}C$-TG)의 생합성은 0.1 M tris-HCl buffer, pH 7.4 및 반응온도 $20^{\circ}C$에서 각각 최고의 합성율을 나타냈고, ATP, coenzyme A (CoASH), $Ca^{2+}$ 및 $Mg^{2+}$의 cofactor 등은 triacylglycerol 생 합성계에 전혀 영향을 끼치지 않았으며, 이들은 phospholipid 생성계를 활성화시키는 것으로 나타났다. 동일 조건하에서 (U-$^{14}C$)-palmitic acid에 의한 중성지 질의 생합성을 비교하여 본 결과, 반응온도는 $20^{\circ}C$가 적정온도였고, 이 반응계는 ATP, CoASH 및 $Mg^{2+}$의 cofactor를 필요로 했는데 반응시간에 따라 차이를 보여주었다. 즉, 30분 반응에서는 cofactor 존재하에서 $^{14}C$-TG 생성이 증가를 보였으나, 3시간 반응에서는 cofactor가 존재하지 않은 대조구보다 $^{14}C$-phospholipids 생성이 증가함을 나타냈다. 간 균질물의 여러 분획에 있어서 $^{14}C$-pal. carn에 의한 $^{14}C$-TG의 생성을 비교해 본 결과, microsomal cytosol 분획에서 가장 높은 triacylglycerol와 diacylglycerol의 생성율을 보였고, 다음이 mitochondria 분획, nuclei와 cell debris 분획 순이었다. 이상의 결과로 보아 palmitoylcarnitine은 microsome에서 cofactor의 도움 없이 직접 acyl group을 이전시켜 중성지질을 생성하는 것을 확인할 수 있었다. It was confirmed that the important metabolic site of acylcarnitine was liver in mamals from the results of its hydrolysis, in which percent of hydrolysis was 33%, 51 %, and 61% from incubation for 1 h, 3 h, and 6 h, respectively. In the triacylglycerol biosynthesis from (U-$^{14}C$)-palmitolyl-DL-carnitine, the optimum conditions were obtained in 0.1 M tris-HCl buffer, pH 7.4 and $20^{\circ}C$ of incubation temperature. The effect of cofactors, such as $Ca^{2+}$, $Mg^{2+}$, ATP, and coenzyme A reduced the formation of triacylglycerol, rather increased the formation of phospholipid. This result showed that triacylglycerol synthesis from palmitolycarnitine did not need any cofactors. The comparison of the triacylglycerol biosynthesis from (U-$^{14}C$)-palmitic acid with that of (U-$^{14}C$)-palmitoyl-DL-carnitine showed the same optimum conditons in pH and incubation temperature. But in cofactors effect, tracylglycerol synthesis from $^{14}C$-palmitic acid was stimulated in the presence of ATP, CoASH, and $Mg^{2+}$ in 30 min incubation when it was compared with or without of cofactors. When the incubation period was prolonged to 3 h, triacylglycerol synthesis was reduced and, on the other hand, phospholipid synthesis was increased significantly more than 6 times. With the results from the different cell fractions, the highest triacylglycerol biosynthesis was prepresented with microsomal cytosol fraction and next was in order of mitochondrial fraction and nuclei cell debris fraction. As conclusion, firstly, it could be postulated that the direct mobilization of acyl group from palmitoylcarnitine to monoacylglycerol and diacylglycerol resulted triacylglycerol synthesis. Secondly, free fatty acid from hydrolysis of palmitoylcarnitine might from acyl CoA in the presence of ATP and CoASH, and continued the sequencial reactions with diacylglycerol and with Iysophospholipid to form the triacylglycerol and phospholipid, respectively.

간호대학생의 불안 및 지각된 스트레스 경험과 자아탄력성과의 관계

이윤경(Lee, Youn-Kyung),김현리(Kim, Hyunli) 한국산학기술학회 2014 한국산학기술학회논문지 Vol.15 No.4

본 연구는 간호대학생의 자아탄력성, 불안, 주관적 스트레스의 정도를 파악하고 이들 간의 관계를 파악하고자 시도 된 서술적 조사연구이다. K시 일개 간호 대학생을 대상으로 2013년 9월 9일에서 14일까지 자료를 수집하였으며 수집된 자료 526부 중 최종적으로 500부가 분석되었다. SPSS 18.0 통계 프로그램에 의해 t-test, ANOVA, Pearson correlation coefficients를 이용하여 대상자의 일반적 특성과 이에 따른 자아탄력성, 불안, 주관적 스트레스의 차이를 알아보고 세 변수 의 관계를 파악하였다. 분석 결과 대상자의 자아 탄력성 평균점수는 3.36(0.50)점, 불안은 2.26(0.70)점, 스트레스는 2.55(0.55)점이었으며, 자아탄력성은 남학생이 여학생보다, 24세 이상 군에서 23세 이하의 군보다 유의하게(p<0.05) 높았 다. 자아탄력성은 불안 (r=- .383, p=0.000) 및 지각된 스트레스와 (r=- .454, p=0.000) 음의 상관관계를 갖는 것으로 나타 났으며 불안과 지각된 스트레스는 강한 양의 상관관계(r= .688, p=0.000)를 나타내었다. 이상의 연구결과를 바탕으로 자아 탄력성이 갖는 긍정적 측면을 인식하고 이를 강화시킬 수 있는 프로그램이나 교과과정의 개발을 제언하는 바이다. This study was conducted to identify the relationship between ego-resilience, anxiety and perceived stress. The participants were 500 students of nursing college located in K city, Korea. Data were collected from September, 9th to 14th 2013, and were analyzed by t-test, ANOVA, Pearson correlation coefficients. The result were as follows; 1) The mean scores were ego-resilience 3.36(0.50), anxiety 2.26(0.70) and stress 2.55(0.55). 2) Ego-resilience was significantly different according to gender, age, satisfaction for major, subjective career opportunity, subjective health. The score were higher at the male group than the female group, and the group who aged over 24 were higher than the group aged below 23. 3) Significant correlations were found between ego-resilience, anxiety and stress. Ego-resilience has negative relationship with level of anxiety(r=- .383, p<0.000) and perceived stress(r=- .454, p<0.000). While, the positive relationship were significant(r= .685, p<0.000) between level of anxiety and perceived stress. According to these result, ego-resilience promotion program is need to develop as a positive concept to adjust college life and nursing course.

옻나무 추출액 섭취와 운동훈련이 흰쥐의 혈중 지질 및 인슐린 저항성에 미치는 영향

이윤경 ( Youn Kyung Lee ),이수천 ( Soo Chun Lee ),전병덕 ( Byung Duk Jeon ),김세현 ( Sea Hyun Kim ),김판기 ( Pan Gi Kim ),류승필 ( Sung Pil Ryu ) 한국산림과학회 2009 한국산림과학회지 Vol.98 No.6

식품소재 물 추출물이 쥐 혈청과 피부의 Testosterone 및 Dihydrotestosteron 함량에 미치는 영향

이윤경 ( Youn Kyung Lee ),김정기 ( Jung Ki Kim ),조종원 ( Jong Won Cho ),김순동 ( Soon Dong Kim ) 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2003 한국식품저장유통학회지 Vol.10 No.1

지구성 운동시 옻나무 추출액의 지질과산화 및 염증성아이토카인 억제효과

이윤경 ( Youn Kyung Lee ),권오선 ( Oh Seon Kwon ),송영주 ( Young Ju Song ),김세현 ( Sea Hyun Kim ),김판기 ( Pan Gi Kim ),류승필 ( Sung Pil Ryu ) 한국산림과학회 2010 한국산림과학회지 Vol.99 No.1

수준별 대학수학 수업의 학습유형 분석에 관한 연구

이윤경(Lee, Youn-Kyung) 한국산학기술학회 2016 한국산학기술학회논문지 Vol.17 No.3

본 연구는 수준별 대학수학교과목을 수강하는 학생들의 학습유형을 분석하여 그 상관관계를 이용하여 보다 효율적인 대학수학 수업이 이루어지기 위한 기초자료를 얻고자한다. 이를 위하여 Kolb 학습유형 분석을 이용하여 일 개 대학교 공과대학의 수준별로 진행되는 대학수학교과목 수업 상하 각 3반 213명을 대상으로 연구를 진행하였고 유의미한 결과를 분석하였다. 연구 결과 첫째, 수준별 수업 중 상반의 학습유형은 확산자, 적용자, 동화자, 수렴자 순이었다. 둘째, 수준별 수업 중 하반의 학습유형은 적용자, 확산자, 동화자, 수렴자 순이었다. 셋째, 상반 학생들의 효율적인 수업을 위해서 교수자는 학생 스스로 수학적 문제를 분석해 볼 수 있는 충분한 시간을 제공해야 한다. 넷째, 하반의 효율적인 수업을 위해서 교수자는 실험적이고 다양한 교수 방법을 이용하여 학생들의 집중력과 학업성취 욕구를 높여야 한다. 이 연구를 바탕으로 대학수학 수업에서 각각 학생들의 학습유형에 적합하고 대학수학 교육과정의 성격에 부합하는 교수법의 개발이 필요함을 제언하였다. This study examined the level-differentiated mathematic classes to offer basic data for effective college mathematics curriculum. Using the Kolb Learning Style, this study surveyed 213 college engineering students in 6 level-differentiated classes in one university and analyzed the significant consequence. The results showed that the ranking of the Learning Style in a superior mathematic class is Diverger, Accommodator, Assimilator, and Converger. Second, the ranking of the Learning Style in the inferior mathematics class was Accommodator, Diverger, Assimilator, and Converger. Third, for effective class of superior mathematics class, professors need to give sufficient time to analyze mathematics problems by the students themselves. Fourth, for an effective class of inferior mathematic class, professors need to use experimental and diverse teaching method to enhance the students' concentration and learning achievement. Based on this study, professors should develop teaching methods that fit the students' Learning Style and the properties of college mathematics curriculum.

빈혈의 원인동정과 면역매개용혈성빈혈의 감별진단

이윤경 ( Youn Kyung Lee ),이채용 ( Chai Yong Lee ),남향미 ( Hyang Mi Nam ),강문일 ( Mun Il Kang ) 한국가축위생학회 2007 韓國家畜衛生學會誌 Vol.30 No.1

Anemia is a common problem in sick dogs, and immune-mediated hemolytic anemia(IMHA) is one of the most common causes of anemia in dogs. Since death can occur rapidly in dogs with IMHA even with appropriate treatment, it is important to differentiate IMHA from other causes of anemia in its first stages. To diagnose underlying diseases in anemic dogs and differentiate IMHA cases from others, 29 patient dogs suffering from severe anemia that had been referred to Veterinary Medical Teaching Hospital at a National University from June 2004 to April 2005 were examined. The most common cause of anemia in the patient dogs was found to be liver disease accounting for 31.0% (9/29) of all, and the second most common was IMHA with 13.7% (4/29). Four dogs confirmed as IMHA cases all reacted positive to direct anti-globulin test and showed spherocytes and polychromatic erythrocytes in the blood smear. Most of the IMHA cases (3/4) were female aged 2 to 7 years and were in a severe state of anemia with less than 20% of PCV.

어머니의 양육효능감 및 언어통제유형과 유아의 탄력성의 관계

이미경(Mi Kyung Lee),이윤경(Youn Kyoung Lee) 한국열린유아교육학회 2014 열린유아교육연구 Vol.19 No.2

본 연구의 목적은 어머니의 영역별 양육효능감 및 언어통제유형과 유아의 다차원적 탄력성의 관계를 규명하는 데 있다. 연구 대상은 충북 청주시에 소재한 17개 어린이집 24학급에 재원하고 있는 만 5세 유아와 그 어머니 각각 399명으로 연구도구는「한국판 양육효능감 척도(K-EGSCP)」(성지현, 백지희, 2011), 「어머니의 언어통제유형 척도」(이진성, 2006)와「유아의 탄력성 척도」(이연실, 2012)가 사용되었다. 수집된 자료 분석을 위해 상관관계분석(Pearson`s Correlation Analysis)을 실시하였다. 본 연구의 결과, 첫째, 훈육, 일상체계조직, 교육과 같은 양육효능감의 하위영역에서 어머니의 양육효능감이 높을수록 어머니들은 명령지향적 언어통제유형을 적게 사용하는 것으로 나타났다. 둘째, 어머니의 양육효능감 하위영역과 유아의 탄력성의 하위영역은 실제적인 관련성이 없는 것으로 나타났다. 그러나 어머니의 훈육영역과 교육영역에 대한 양육효능감이 높을수록 유아의 능력적 탄력성의 문제해결력이 높게 나타났다. 셋째, 어머니의 언어통제유형과 유아의 탄력성 하위영역과 하위요인은 관련성이 없는 것으로 나타났다. 본 연구 결과와 논의를 종합해 볼 때 어머니의 양육효능감의 훈육, 일상체계 영역 및 교육영역과 어머니의 명령지향적 언어통제유형은 서로 관계 변인이다. 또한 어머니의 양육효능감의 훈육영역과 교육영역은 유아의 능력적 탄력성의 문제해결력과 서로 관련변인임을 알 수 있다. 따라서 어머니의 양육효능감이 갖는 영역특수성은 어머니의 언어통제유형과 유아의 탄력성 발달에 영향을 미치는 중요한 변인임을 알 수 있다. This study aims to determine the relationships among mothers` parenting efficacy, verbal control styles, and children`s resilience. The subjects of the study consist of 399 five-year-olds and their mothers attending a childcare center in Cheongju city, Chongcheongbuk-do, Korea. The research instruments used for the study were「the Echelle Globale du Sentiment de Competence Parentale-Korean version (K-EGSCP)」(Sung & Baek, 2011),「An Inventory for Measuring Mothers` Verbal Control Styles」(Lee, 2006), and「Child`s Resilience Inventory」(Lee, 2012). The collected data were analyzed by Pearson`s Correlation Analysis using SPSS 16.0. The results are as follows. First, the relationship between mothers` parenting efficacy and imperative-oriented verbal control mode showed a negative correlation, especially in the sub-items of disciplines, instrumental care, and teaching. Second, the sub-areas of children`s resilience demonstrated no correlation with the mothers` parenting efficacy. But in particular, there was a positive correlation between the sub-areas of mothers` parenting efficacy (e.g., discipline and teaching) and the sub-construct of competent resilience (e.g., problem solving). Third, there was no correlation between mothers` verbal control styles and children`s resilience.

Rainbow Power Therapy의 경항통에 대한 효과

서정철,서보명,김성웅,이경민,윤종석,이세연,김경운,이윤경,임성철,정태영,황재옥,한상원,Seo, Jung-Chul,Seo, Bo-Myung,Kim, Sung-Woong,Lee, Kyung-Min,Yun, Jong-Seok,Lee, Sea-Youn,Kim, Kyung-Woon,Lee, Yoon-Kyung,Lim, Seong-Chul,Jung, Tae-Young 경락경혈학회 2004 대한침구의학회지 Vol.21 No.2

Objective : This study was designed to estimate the effects of Rainbow Power therapy on neck pain patients by using Visual Analogue Scale(VAS) and pressure algometer. Methods : Rainbow Power therapy group consisted of 18 patients and acupuncture therapy group consisted of 7 patients. The degree of improvement of neck pain was evaluated by VAS and pain pressure threshold(at the points of Jianjing GB21 and Jianwaishu SI14) before treatment, before 3rd treatment and before 5th treatment. Rainbow Power therapy was performed at the points of Jianjing GB21, Zhongfu LU1, Yangxi LI5, Ganshu BL18, Weishu BL21 and Shenshu, BL23. Each points were stimulated with RP-UM103(Rainbow Power therapy instrument) for 20 seconds. The points of acupuncture therapy were Jeonggeun, Jeongjong, Sangbaekn and Hegu LI4 and acupuncture was maintained for 15 minutes. After above therapy dry cupping was performed at the points of Jianjing GB21, Jianwaishu SI14, Gaohuang BL43, Tianzong, SI11 and Bingfeng SI12 for 5 minutes. Results : There was no significant difference between the two groups in VAS, pain threshold of Jianjing GB21 and Jianwaishu SI14 following treatment. In the two groups VAS was significantly decreased. In Rainbow Power therapy group the pain threshold of the two points was significantly increased. But there was no significant increase in acupuncture therapy groups about the pain threshold of Jianwaishu SI14. Conclusions : The effectiveness of Rainbow Power therapy on neck pain was shown through VAS and pressure algometer. These imply that Rainbow Power therapy may be useful for neck pain. Further study is needed about Rainbow Power therapy.

Rainbow Power Therapy의 경항통에 대한 효과

서정철 ( Jung Chul Seo ),서보명 ( Bo Myung Seo ),김성웅 ( Sung Woong Kim ),이경민 ( Kyung Min Lee ),윤종석 ( Jong Seok Yun ),이세연 ( Sea Youn Lee ),김경운 ( Kyung Woon Kim ),이윤경 ( Yoon Kyung Lee ),임성철 ( Seong Chul Lim ) 대한경락경혈학회 2004 Korean Journal of Acupuncture Vol.21 No.2

Objective: This study was designed to estimate the effects of Rainbow Power therapy on neck pain patients by using Visual Analogue Scale(VAS) and pressure algometer. Methods: Rainbow Power therapy group consisted of 18 patients and acupuncture therapy group consisted of 7 patients. The degree of improvement of neck pain was evaluated by VAS and pain pressure threshold(at the points of Jianjing GB21 and Jianwaishu SI14) before treatment, before 3rd treatment and before 5th treatment. Rainbow Power therapy was performed at the points of Jianjing GB21, Zhongfu LU1, Yangxi LI5, Ganshu BL18, Weishu BL21 and Shenshu, BL23. Each points were stimulated with RP-UM103(Rainbow Power therapy instrument) for 20 seconds. The points of acupuncture therapy were Jeonggeun, Jeongjong, Sangbaekn and Hegu LI4 and acupuncture was maintained for 15 minutes. After above therapy dry cupping was performed at the points of Jianjing GB21, Jianwaishu SI14, Gaohuang BL43, Tianzong, SI11 and Bingfeng SI12 for 5 minutes. Results: There was no significant difference between the two groups in VAS, pain threshold of Jianjing GB21 and Jianwaishu SI14 following treatment. In the two groups VAS was significantly decreased. In Rainbow Power therapy group the pain threshold of the two points was significantly increased. But there was no significant increase in acupuncture therapy groups about the pain threshold of Jianwaishu SI14. Conclusions: The effectiveness of Rainbow Power therapy on neck pain was shown through VAS and pressure algometer. These imply that Rainbow Power therapy may be useful for neck pain. Further study is needed about Rainbow Power therapy.