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폐암환자의 객담 및 암 조직에서 MAGE A 1-6와 SSX 1-9 유전자의 발현
이연재 ( Yeun Jae Lee ),이장훈 ( Jang Hoon Lee ),이정철 ( Jung Cheul Lee ),이관호 ( Kwan Ho Lee ),LungNeoplasmsGenes 대한결핵 및 호흡기학회 2011 Tuberculosis and Respiratory Diseases Vol.70 No.4
Background: A variety of diagnostic modalities for lung cancer have been developed. To achieve efficient and early detection of lung cancer, we tried to measure the expression rates of the melanoma associated gene (MAGE) and synovial sarcoma on X chromosome (SSX) genes. Methods: We designed primers for the SSX gene. In addition to the pre-developed MAGE A primer, using an SSX gene primer was attempted to increase the detection rate. We obtained cancer tissues and cancer-free lung tissues from resected lung, sputum from lung cancer patients who had not undergone surgery, and sputum from healthy people and patients with benign intrathoracic diseases. Results: The sensitivity of the MAGE or SSX gene RT-PCR to identifying cancer tissue of the 69 lung cancer patients was 95.2% for squamous cell carcinoma (scc), 87.0% for adenocarcinoma, and 100% for small cell carcinoma. The mean sensitivity value was 94.2% (p=0.001). For adenocarcinoma, the additional use of the SSX gene resulted in a higher expression rate than MAGE alone (87% vs. 69.6%). The expression rate for the cancer-free lung tissue was 14.3% in scc, 17.4% in adenocarcinoma, and 25.0% in small cell carcinoma. In the induced sputum of 49 lung cancer patients who had not undergone surgery, the expression rate for one of the two genes was 65.5%. The expression rate for the sputum of healthy people and benign intrathoracic diseases by MAGE or SSX gene reverse transcription polymerase chain reaction (RT-PCR) was 3.8% and 17.7%. Conclusion: Detecting lung cancer using the expression of MAGE and SSX genes in lung cancer tissue has high sensitivity.
한국인 역류 식도염 환자의 식도 상피 세포 사이 간격 확장과 Esomeprazole 투여 후의 변화
김재환 ( Jae Hwan Kim ),지삼룡 ( Sam Ryong Jee ),박성재 ( Sung Jae Park ),양성연 ( Sung Yeun Yang ),박은택 ( Eun Taik Park ),이연재 ( Youn Jae Lee ),이상혁 ( Sang Hyuk Lee ),설상영 ( Sang Yong Seol ),정정명 ( Jung Myung Chung ) 대한소화기기능성질환·운동학회 2006 Journal of Neurogastroenterology and Motility (JNM Vol.12 No.1
목적: 서구에서 역류 식도염 환자의 식도 상피는 세포 사이 간격이 확장되어 있으며, 이는 프로톤 펌프 억제제 치료 후 호전된다고 한다. 이번 연구의 목적은 우리나라 역류 식도염 환자에서 식도 상피 세포 사이 간격이 확장되어 있는지 여부와 프로톤 펌프 억제제 치료 후 세포 사이 간격이 호전되는지 알아보았다. 대상 및 방법: 역류 식도염 증상이 있으며 상부위장관내시경 검사에서 점막 손상이 있는 역류 식도염 환자 10명과 위식도역류의 증상이 없고, 상부위장관내시경 검사에서 점막 손상이 없으며 24시간 보행 식도 산도 검사에서 음성인 정상인 10명을 대상으로 하였다. 모든 대상자에서 상부위장관내시경 검사를 시행하였으며, 위식도 경계부 5㎝ 상방에서 4군데 조직을 생검하여 전자 현미경으로 세포 사이 간격을 측정하였다. 모든 식도 조직의 전자 현미경 소견에서 100 번씩 세포 사이 간격을 측정하였다. 역류 식도염 환자는 8주간 esomeprazole로 치료한 후 내시경 검사를 하고 식도 상피 조직을 생검하여 세포 사이 간격을 다시 측정하였다. 결과: 역류 식도염 환자의 세포 사이 간격(평균 1.49±0.39 ㎛)은 대조군의 식도 상피 세포 사이 간격(평균 0.49±0.10 ㎛)보다 통계적으로 유의하게 확장되었다(p<0.01). 역류 식도염 환자에서 esomeprazole로 치료 후 세포 사이 간격(평균 0.56±0.19 ㎛)은 호전되었다(p<0.01). 결론: 우리나라 역류 식도염 환자의 식도 상피 세포 사이 간격은 정상인에 비해 확장된 소견을 보이며, 프로톤 펌프 억제제 치료 후 호전되었다. Background/Aims: It has been demonstrated that dilated intercellular spaces of esophageal epithelium are a marker of tissue injury in Western gastroesophageal reflux disease (GERD); which heals after proton pump inhibitor (PPI) treatment. The aim of this study was to determine whether dilated intercellular spaces are also a feature of acid damage in Korean GERD patients and are also healed after PPI treatment. Methods: Ten GERD patients with heartburn and esophageal mucosal breaks on endoscopy, and 10 controls with no symptoms, normal mucosa on endoscopy and negative 24-hour pH monitoring were enrolled. During upper gastrointestinal endoscopy, four biopsies from normal mucosa were taken within the lower 5 ㎝ of the esophagus for transmission electron microscopy (TEM) analysis. One hundred computer measurements were taken on TEM photomicrographs of the specimens in each patient. After eight weeks of esomeprazole 40 ㎎/day, patients with erosive esophagitis had another endoscopy with biopsies and the intercellular spaces were measured again. Results: The mean intercellular space diameter, in the erosive esophagitis patients, was greater than in controls (1.49±0.10 ㎛ vs 0.49±0.10 ㎛, p<0.01). The mean intercellular space diameter, in erosive esophagitis patients, improved after PPI treatment. Conclusions: Korean erosive esophagitis patients have dilated intercellular spaces of esophageal epithelium and dilated intercellular spaces can be reversed after esomeprazole treatment. (Kor J Neurogastroenterol Motil 2006;12:19-24)
한국인 B형 간염 바이러스 관련 간질환에서 HBV C 및 S 유전자 영역 돌연변이의 면역학적 중요성
정정명 ( Jung Myung Chung ),조환진 ( Hwan Jin Cho ),이연재 ( Yeon Jae Lee ),장윤식 ( Yeun Sik Jang ),박은택 ( Eun Taek Park ),최봉기 ( Bong Ki Choi ),모혜경 ( Hye Kyoung Mo ),박영홍 ( Yeong Hong Park ) 대한소화기학회 2002 대한소화기학회 춘계학술대회 Vol.2002 No.-
<목적> 본 연구의 목적은 HBV DNA의 C와 S 유전자 영역에서 돌연변이의 빈도를 관찰하고 면역 상태와 C와 S 유전자 영역의 돌연변이 사이에 상관관계를 알아보고자 한다. <방법> HBV 관련 간질환 소아와 성인 34명으로부터 추출한 혈청이나 생검 간조직으로부터 DNA을 추출하였다. 추출한 DNA로부터 HBV의 C와 S 유전자 영역을 증폭하고 클로닝하여 염기서열을 분석하였다. <결과> 34례의 adr type의 분석 결과 32례(94.1%)에서 co
조경임(Kyoung Im Cho),윤철(Cheol Yoon),이홍기(Hong Gi Lee),김석주(Seok Joo Kim),장윤식(Yeun Sik Jang),이연재(Youn Jae Lee),이상혁(Sang Hyuk Lee),설상영(Sang Young Seol),정정명(Jung Myung Chung),강미선(Mi Seon Kang) 대한소화기학회 2000 대한소화기학회지 Vol.36 No.3
Peripheral cholangiocarcinoma which was originated from the small bile ducts and generally presents an infiltrative growth, intraductal growth in the peripheral large duct tributaries, is very unusual. Mucin-producing cholangiocarcinoma showing intraductal spread has been reported previously, and it is difficult to diagnose this type of cholangiocarcinoma definitely in early stage in spite of jaundice due to mucin impaction in the common bile duct. We experienced a 49-year-old male patient who had hepatic mass observed incidentally without any specific symptom. This patient was diagnosed as having mucin-producing cholangiocarcinoma by abdominal ultrasonography, computed tomography scan and ERCP. He received hepatic resection successfully. It was diagnosed as mucin-producing cholangiocarcinoma, intrahepatic variant. He is expected to have long term survival. We report this case because the case of asymptomatic mucin-producing cholangiocarcinoma diagnosed at such an early stage is rare. (Kor J Gastroenterol 2000;36:431 - 436)
B형 만성 간질환 환자에서 HBV core promoter 변이에 대한 고찰
김호철,이연재,장윤식,이상혁,설상영,박영흥,정정명 인제대학교 2000 仁濟醫學 Vol.21 No.1
Objectives: Chronic hepatitis B virus(HBV) infection may cause various kinds of liver disease. The pathophysiologic mechanisms of such clinical manifestations are still not clearly understood. According to the recent reports, mutations in the HBV core promoter may have an impact on the progression of HBV-related liver disease. The aim of this study was to clarify the frequency and location of naturally occurring mutations in the core promoter region of HBV, and their possible effects on the clinical course of HBV -associated chronic liver diseases. Methods: and Materials: HBV DNA was extracted from the sera of 31 patients who had been HBsAg positive for more than 6 months. The core promoter/precore sequence was amplified via polymerase chain reaction, and direct sequenced. And in this study. 31 patients were divided into 3 groups according to the histopathological severity of liver disease i.e. Group 1 as minimal change and chronic persistent hepatitis, Group 2 as chronic active and lobular hepatitis, Group 3 as liver cirrhosis and hepatocellular carcinoma, respectively. Results: 1.Frequent mutated nucleotides(nt) were 1764(77.4%), 1762(58.1%) and 1753 (25.8%). 2.A double-nucleotide mutation at nt1762 and nt1764 (M1) was observed in 18 cases of 31 patients(58.1%), a single-nucleotide mutation at nt1764(M2) in 5 cases. But a single-nucleotide mutation at nt1753 or nt1762 were not observed at all. 3.M1 had nothing to do with the severity of hepatitis. In case of M2. there was association with severity of hepatitis only in comparisons among Group 2 vs. Group 3(p<0.05) 4.The frequency of mutation in core promoter region had little association with the status of HBeAg/Anti-HBe. 5.The frequency of mutation at nt1896(G→A) in precore region was related with status of HBeAg/Anti-HBe(p<0.01) rather than the severity of disease. Conclusion: We could not find the definite relationship between core promoter mutation and the histopathologic severity of HBV associated liver disease. Further investigations are needed with larger number of patients.
설상훈,엄수정,이연재,최봉기,박은택,장윤식,이상혁,설상영,정정명 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.1
■ Objectives The aim of this study was to evaluate the biochemical and virological responses to one year lamivudine therapy in HBV-associated chronic liver diseases including interferon(IKF) -naive. INF-failed chronic hepatitis and liver cirrhosis. ■ Methods This study was conducted as an one-year trial of lamivudine in 60 patients with HBV-associated liver disease(INF-naive chronic hepatitis 20. INF-failed chronic hepatitis 18. liver cirrhosis 22). We classifed the patients as INF-naive chronic hepatitis(Group A), as INF-failed chronic hepatitis(Group B) and as liver cirrhosis(Group C). ■ Results The mean normalization rate of serum ALT was 71.7%: 65.0% in Group A. 61.1% in Group B and 86.4% in Group C. The negative conversion rate of HBV-DNA was 73.3%: 60.0% in Group A, 83.3% in Group B and 77.3% in Group C. The seroconversion rate of HBeAg was 30.0%: 22.2% in Group A, 35.3% in Group B and 26.3% in Group C. The breakthrough was occurred in 26.7% of patients: 40% in Group A, 16.7% in Group B and 22.7% in Group C. However, they did not deteriorate clinically in spite of ALT elevation and HBV-DNA reappearance. In case of anti-HBe positive chronic liver disease, ALT was normalized and HBV-DNA became undetactable in all 6 patients after 1 year lamivudine treatment. ■ Conclusions One year lamivudine treatment resulted in biochemical and virological improvements in patients with HBV- associated liver disease irrespective of the severity of liver disease and the status of HBeAg/Anti-HBe. Further studies are required to determine the effects of long-term suppressive therapy and optimal duration of therapy in patients with chronic HBV infection.
김호철,장윤식,이연재,이상혁,설상영,정정명,최하진,양영일 인제대학교 1996 仁濟醫學 Vol.17 No.2
원발성 담즙성 간경변증은 원인 미상의 담즙정체 질환으로 만성적인 간내 담관의 염증을 보이면서 간경변증으로 진행하는 드문 질환이다. 저자들은 황달과 소양증을 주소로 내원한 55세의 여자 환자에서 원발성 담즙성 간경변증 1례를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Primary biliary cirrhosis is rare and a disease of unknown origin. It is a chronic cholestatic liver disease characterized by the presence of chronic inflammation, necrosis of hepatic bile duct and progression to liver crirhosis. We experienced a 55 year- old woman complained of jaundice and itching for 3 years. Her clinical symptoms, biochemistry, liver function test and liver biopsy were compatible with primary biliary cirrhosis. Thus we report a case of primary biliary cirrhosis with literature review.