同軸 對向제트의 亂流特性 硏究 : 동축 대향제트의 난류특성 연구

이상수 釜山大學校 工科大學 1980 機械技術硏究所 硏究報告 Vol.2 No.-

K11 RNA 중합효소의 Cloning 및 발현

이상수,Lee, Sang-Soo 배재대학교 자연과학연구소 1997 自然科學論文集 Vol.9 No.1

PCR 방법을 이용하여 K11 RNA 중합효소를 coding하는 Klebsiella phage gene 1을 cloning 하였고 lac 전사촉진제 조절 하에 발현시켰다. K11 RNA 중합효소는 DAEA-sephacel과 Affigel blue column chromatographies를 사용하는 상용 방법으로 분리하였다. DAEA-sephacel의 0.2-0.3 M $NH_4Cl$ 분획에서 K11 RNA 중합효소의 활성을 보였고, 다음 단계의 Affigel blue column에서 SDS-polyacryl amide gel 상의 단일 band로 분리되었다. K11 RNA 중합효소는 T7 그룹 phage RNA 중합효소로 다른 T7 그룹phage RNA 중합효소와 많은 상동성을 보인다. (대장균 phage T7, T3과 Salmonella tyhimurium phage SP6 RNA 중합효소). 이미 우리는 T7과 SP6 전사촉진제 변이체를 제조한 바 있고 T7과 SP6 RNA 중합효소의 전사촉진제 특이성을 연구한 바 있다 (이상수와 강창원, 1993). K11 RNA 중합효소의 전사촉진제 특이성을 알아보기 위해 SP6 전사촉진제 변이체를 사용하여 in vitro K11 RNA 중합효소의 활성을 측정하였다. 이 변이체 중 K11 전사촉진제와 가장 유사한 것이 가장 높은 K11 RNA 중합효소 활성을 보였다. Using the PCR(polymerase chain reaction method), gone 1 of phage K11 coding for K11 phage RNA polymerase has been cloned and expressed under the control of lac promoter. K11 phage RNA polymerase was conventionally purified through the DEAE-sephacel and Affigel blue column chromatographies. The 0.2-0.3 M $NH_4Cl$ fractions of DAEA-sephacel column chromatography showed K11 phage RNA polymerase activity and further purification with Affigel blue column chromatography showed nearly single protein band on SDS-polyacryl amide gel. K11 phage RNA polymerase, which is one of the T7 group phage RNA polymerase (E. coil phage T7, T3 and Salmonella tyhimurium phage SP6 RNA polymerase), shares high degrees of homology with the other T7 group phage RNA polymerase. Previously we constructed T7 and SP6 promoter variants and revealed promoter specificity of T7 and SP6 RNA polymerase (Lee and Kang, 1993). To investigate the promoter specificity of K11 RNA polymerase in vitro K11 promoter activity was measured with SP6 promoter variants. The SP6 promoter variant share highest degrees of sequence homology with K11 promoter sequence show strongest promoter activity.

The Synchronous Prevalence of Colorectal Neoplasms in Patients with Stomach Cancer

이상수,정운태,김차영,하창윤,민현주,김현진,김태효 대한대장항문학회 2011 Annals of Coloproctolgy Vol.27 No.5

Purpose: The association between stomach cancer and colorectal cancer is controversial. The purpose of this study was to determine the synchronous prevalence of colorectal neoplasms in patients with stomach cancer. Methods: A total of 123 patients with stomach cancer (86 male) and 246 consecutive, age- and sex-matched persons without stomach cancer were analyzed from July 2005 to June 2010. All of them underwent colonoscopy within 6 months after undergoing gastroscopy. Results: The prevalence of colorectal neoplasms was significantly higher in the stomach cancer group (35.8%) than in the control group (17.9%) (P < 0.001). Colorectal neoplasms were more prevalent in the patients with stomach cancer (odds ratio [OR], 3.10; 95% confidence interval [CI], 1.71 to 5.63). In particular, the difference in the prevalence of colorectal neoplasms was more prominent in the patients above 50 years old (OR, 3.54; 95% CI, 1.80 to 6.98). Conclusion: The results showed that the synchronous prevalence of colorectal neoplasms was higher in patients with stomach cancer than in those without stomach cancer. Therefore, patients with stomach cancer should be regarded as a high-risk group for colorectal neoplasms, and colonoscopy should be recommended for screening.

코비디엔혁신센터로 살펴본 의료기기산업의 외국직접투자

이상수,이무열 대한의사협회 2015 대한의사협회지 Vol.58 No.10

Medical device industry has characteristics of multi-disciplinary convergence and is easy to apply with information communication technology. Such nature makes Korea medical device industry more globally competitive than the pharmaceutical industry. The foreign direct investment (FDI) of service industry in Korea continues to be at a standstill and the medical device industry is not an exception. Among this stagnant FDI situation in Korea, Covidien, a global medical device company has established the Covidien Center of Innovation as both an education and training facility for domestic and foreign healthcare professionals and a medical device research and development (R&D) center in August 2013. Along with the global attractiveness of superior clinical outcomes generated by healthcare professionals, the future medical device industry development will be fueled by continued R&D investments, researchoriented hospital R&D activities, and clinical studies, etc. It is important to provide attractive investment environments to embrace FDI similar to Covidien Center of Innovation into Korea in order to leverage healthcare professionals’ competency at maximum and lead collaboration between medical device companies and healthcare professionals. It is imperative that ‘innovative medical device enterprise’ certification system similar to the curent ‘innovative pharmaceutical enterprise certification’ is created. If additional premium reimbursement prices are granted to medical devices developed and manufactured by the innovative enterprises which contribute to domestic R&D and healthcare professional education and training through FDI in Korea, it will help FDI activation in Korea to a great extent. And the reimbursement coverage for routine care costs in clinical trial will stimulate FDI in Korea.

ドラマを活用した日本語教育 - 自己評価とコミュニケーション教育を目指して

이상수 동북아시아문화학회 2014 동북아 문화연구 Vol.1 No.39

This study solved Japanese communication to Japanese learner of Korean. In addition, this research studied introducing drama and this is influential to Japanese communication. This research sought for effective Japanese education and suggested definite goal. 1) Japanese communication between grammar education and Japanese education: Grammar and Japanese education make effective education of communication true. 2) communication of Japanese education by suitable grammar education: Making education which would be exploited in actual communication by mixing correct expression of grammar. 3) communication of Japanese education which is able to use correct vocabulary: Rendering vocabulary education and using correct word in situation of communicating. 4) Japanese education of communication in relationship with others: Educating language distinction in situation which same vocabulary is exploited in different way. 5) Japanese education of communication in suitable situation: Educating distinction about same expression can be used for different ways. 6) Further assignment: researching Japanese education of communication vertically and build definite and actual Japanese education.

The Six-Party Talks at the Cross-roads: Implications from Eight Years of Experience

이상수,백찬영 국방대학교 국가안전보장문제연구소 2011 The Korean Journal of Security Affairs Vol.16 No.1

The objective of this article is to analyze the Six-Party Talks process using James Mahoney’s path dependence theory as its theoretical framework. The “path” of the Six-Party Talks process will be divided into two types of sequences to explain the timing and the events of each sequence. The sequences include various phases of the talks in process and during the period of the discontinuation of the talks. This article presents an overview of the theoretical background of path dependence theory. This article then demonstrates that path dependence theory can explain how the Six-Party Talks have been maintained and discontinued. This theoretical approach utilizes two elements for analysis: self-reinforcing sequences and negative feedback. In self-reinforcing sequences, an institutional pattern, once adopted, delivers increasing benefits with its continued adoption, and thus over time it becomes more and more difficult to transform the pattern or select previously available options, even if these alternative options would have been more “efficient.” Negative feedback is one of non-repeating sequences in a wider set of mechanisms behind path dependence. Each element can be categorized in terms of utilitarian, functional, power, and legitimation explanations. This article highlights how negative feedbacks have influenced the dynamics between the two self-reinforcing sequences that defined the process to this day. A path dependence approach to the Six-party Talks emphasizes the importance of timing in a path dependent process and the limitations of self-reinforcing sequences. Lastly, this article accounts for the limitations of path dependence theory and the current Six-Party Talks in terms of multilateralism in Northeast Asia.

사법부에 바란다

이상수 한국사법행정학회 1988 사법행정 Vol.29 No.8

MgO 박막의 전자 특성

이상수,유스 라마 데니,이선영,이강일,강희재,박남석 한국물리학회 2012 새물리 Vol.62 No.5

In an AC-PDP (Alternating Current-Plasma Display Panel), a MgO thin film has been used as a protective layer in the discharge cell of the PDP because of its high secondary electron emission. In this work, the electronic properties of MgO thin films grown on Si substrates by using electron beam evaporation were investigated by using X-ray photoelectron spectroscopy (XPS), Reflection electron energy loss spectroscopy (REELS) and ultra violet photoelectron spectroscopy (UPS). MgO thin films grown at room temperature showed F-center defect states due to oxygen vacanc, which were not observed in the MgO thin films grown at 300℃. The band gap obtained for MgO thin films grown at 300℃ was 7.5 eV in both the surface and the bulk while the band gaps obtained for MgO thin film growns at room temperature were 5.9 eV in the surface and 7.5 eV in the bulk. The work functions of the MgO thin films grown at room temperature and 300 ℃ were 4.7 eV and 5.2 eV, respectively. AC-PDP(Alternating Current-Plasma Display Panel) 에서 MgO 박막은이차 전자 방출이 높고 이온 스퍼터링에 의한 표면 손상이 적어보호막으로 이용된다. 이번 연구에서 전자 빔 증착 방식으로 Si 기판위에 MgO 박막을 성장시킨 후, 전자 특성을 XPS, REELS, UPS 실험을통하여 측정하였다. 실온에서 성장한 박막에서는 산소의 결함에 의한F-Center의 준위가 관찰되었으나, 시료를 300 ℃이상에서 가열한것으로 인해 F-Center의 준위가 사라지는 것이 관찰되었다. 실온에서성장시킨 박막의 경우 표면과 내부에서 띠틈은 각각 5.9 eV와 7.5eV이며, 일함수는 4.7 eV이다. 한편 300 ℃에서 성장시킨박막에서 표면과 내부에서 조성비가 균질하며, 띠틈은 7.5 eV 이며,일함수는 5.2 eV 이다.

DTNB oxidation effects on T-type Ca^(2+) channel isoforms

이상수,강호원,박진용,이정하 한국통합생물학회 2011 Animal cells and systems Vol.15 No.2

Redox regulation is one of the ubiquitous mechanisms to modulate ion channels. We here investigated how 5,5'-dithio-bis (2-nitrobenzoic acid), a cysteine specific oxidizing reagent, modulates Ca_v3.1 and Ca_v3.2 T-type Ca^(2+)channels expressed in Xenopus oocytes. Application of the reagent inhibited Ca_v3.1 and Ca_v3.2 currents in a dosedependent manner. The oxidizing reagent (1 mM) reduced the peak amplitude of Ca_v3.1 and Ca_v3.2 currents by 50% over 23 minutes and the decreased currents were fully recovered upon washout of it. The reagent slowed the activation and inactivation kinetics of Ca_v3.1, Ca_v3.2, and Ca_v3.3 channel currents. Notably, the reagent positively shifted both activation and steady-state inactivation curves of Ca_v3.1, while it did not those of Ca_v3.2. Utilizing chimeric channels from Ca_v3.1 and Ca_v3.2, we localized the domains III and IV of Ca_v3.1 responsible for the positive shifts of channel activation and steady-state inactivation. These findings provide hints relevant to the electrophysiological and molecular mechanisms accounting for the oxidative regulation of T-type channels.

Omnivorous Consumer or Omnivorous Producer?: Patterns of Cultural Participation in Korea

이상수,최샛별,이명진 서울대학교 사회발전연구소 2015 Journal of Asian Sociology Vol.44 No.1

Studies on cultural omnivores have focused on consumption activities, largely overlooking cultural production activities. However, consumption and production activities are both essential fields of cultural participation. Through the use of comprehensive data about cultural consumption and production activities, this study attempts to explore patterns of cultural participation in Korea. It also investigates the determinants of these different patterns. Four major findings resulted from the study. First, four different types of cultural participation were derived: omnivorous prosumers, omnivorous consumers, omnivorous producers, and univores. Second, members of the new middle class, with higher educational levels and higher household incomes, are more likely to be omnivorous consumers or omnivorous prosumers. Third, demographic factors such as gender and age also have effects on the patterns of cultural participation in Korea. Lastly, it was revealed that the larger an individual’s social network, the greater the probability of being a cultural omnivore of any type. This study contributes to the expansion of the boundaries of prior discussions on cultural omnivores.