액체크로마토그래피-질량분석기를 이용한 쌀 중 스테리그마토시스틴과 아플라톡신 시험법 개발

이봄내 ( Bom Nae Lee ),권진욱 ( Jin-wook Kwon ),정은아 ( Eun A Chong ),천소영 ( So Young Chun ),강길진 ( Gil Jin Kang ) 한국환경농학회 2019 한국환경농학회 학술대회집 Vol.2019 No.-

Sterigmatocystin is a mycotoxin produced by fungi of the genus Aspergillus (mainly A. nidulans and A versicolor). Sterigmatocystin shares its biosynthetic pathway with aflatoixns. The aim of this study is to optimize the liquid chromatography-tandem mass spectrometry method for determination of sterigmatocystin and aflatoxins (B₁,B₂,G₁,G₂)inrice, simultaneously. Targetsampleswereextractedwith0.1 % formic acid in 50 % acetonitrile then purified with Isolute cartridge (solid phase extraction). Separations by HPLC were mainly conducted with a Cadenza C18 analytical column using 0.1% formic acid and 5 mM ammonium formate under gradient condition with ultrapure water and methanol. The mycotoxins were detected by electrospray ionization in positive or negative ion with multiple reaction monitoring (MRM) and mass spectrometric conditions were optimized in order to increase selectivity and sensitivity. The developed method was validated in terms of linearity, trueness, precision, limits of detection and quantification. Matrix-matched calibration was used for quantification, with regression coefficients (R2) ≥ 0.98.

뇌졸중 환자의 자가간호 수행정도와 가족의 교육적 요구에 관한 연구 : Care and Their Family Members' Needs for Education

이은화,김진숙,박은주,박혜진,최봄내 이화여자대학교 간호과학연구소 2002 Health & Nursing Vol.14 No.2

About 25% of the stroke patients die within a month after attack, and even the patients who survive will have motor, sensory, cognitive dysfunction or speech defect, and therefore, they should continue to be taken care of. Under such circumstances, this study was aimed at reviewing how the stroke patients requiring a long-term nursing were caring themselves and what were their family members’ needs for education and thereby, explore the ways for the nurse to intervene in their care. To this end, a questionnaire survey was conducted by sampling the stroke patients hospitalized at‘E’University hospital in Seoul for 3 or 4 days their 75 family members. The data were collected for 57 days form July 14 to September 8, 2001. The stroke patients’self-care measured b using and ADL scale developed b Kang Hyeon-sook(1984), while their patients’needs were measured by using a scale developed by Han Jeong-seok and Seo Mi-hye(1992) and complemented by Kim Mi-hee (1997) and adapted by the researcher for this study. The collected data were analyzed using SPSS/PC+ 10.0 program for real numbers, percentages, means, standard deviations, ANOVA and Pearson’s coefficients. The results of this study can by summarized as follows; 1) The stroke patients' self-care scored 2.52(±1.02) out of 4 perfect score. 38.1% of the patients were 'completely dependent’on their family members for treatment, while 64.2% of them felt it difficult to nurse themselves. 2) Patients’family members’needs for education scored 4.11(±.78) out of 5 perfect score. The factor which scored highest was 'information related with treatment’(4.39±.76), followed by ‘nursing requirement due to immovability’(4.05±.91), 'nursing related with ordinary life’(3.99±.85) and‘social, psychological and financial problems’3.92±.04) in their order. 3) When analyzed in terms of patients’demographic variables and characteristics of the disease, patients’self-care was significantly higher when there were no grown-up children[F(1, 73)=4.115, p=0.46], but there was found some significant difference depending on the number of troubles[F(5,73)=2.562, p=0.35]. But the patients'self-care was significantly lower when they suffered from a paralysis[F(1, 73)=4.072, p=.047] or when they suffered form a cerebral troubles[F(1, 73)=19.410, p=.000]. When analyzed in terms of patients’demographic variables and disease types, their family members’needs for education were highest when the patients suffered from both Middle Cerebral artery(MCA) and Brain Stem(B.S.) symptom, followed by when they suffered form MCA and when they suffered from B.S. trouble in their order.[F(2, 73)4.092, p=.021]. On the other hand, their needs for education were also high when the patients suffered from a paralysis[F(1, 73)=5.983, p=.017] and a speech defect.[F(1. 73)=4.572. p=.036]. 4) When analyzed in terms of family members’demographic variables, it was found that their educational needs was positively correlated with their educational background[F(3, 73)=3.731, p=.015] and religion, in particular Buddhism.[(4, 73=4.208, p=.004). 5) The stroke patients’self-care was negatively but not significantly correlated with their family members’needs for education.(r=-.113, p=.334). As discussed above, it was confirmed through this study that stroke patients and their family members were much concerned about the prognosis of the disease and self-care, while the family members' needs for education were very high beginning form the time when the patients were hospitalized. Therefore, it is necessary to develop an stroke patients' educational program in consideration of their family members' needs for education and priorities, while exploring an effective way to educate their family members about self-care at the initial stage of hospitalization. All in all, it is also deemed necessary to review the current nursing curriculum about nursing intervention and education of family members for stroke patients and thereby, perform and effective nursing intervention for them.

융기성 피부 섬유육종 1예

이정회,박찬호,한상훤,김태균,윤기현,최동진,문봄내,양윤식,손창학 인제대학교 백병원 2001 仁濟醫學 Vol.22 No.2

Dermatofibrosarcoma protuberans(DFSP) is an uncommon cutaneous tumor, which is characterised by slow invasive growth, marked tendency of local recurrence after excision and infrequent metastasis. Initially, most DFSPs are noticed as plaque like areas of cutaneous thickening and frequently have a reddish at the margins. As the tumor increases in size it is typically described as a raised, firm, multinodular mass that is fixed to the overlying skin, but movable over the deeper tissues. The authors report a case of recurrent DFSP, developed on the right anterior chest of a 32-year-old man. There was no evidence of metastasis.

Methamphetamine 중독증상을 보인 환자에서 발생한 횡문근 융해증 및 급성 신부전 1예

권태경,문봄내,최동진,윤기현,김태균,김경욱,김철홍,김민호,이성주,김영훈 인제대학교 백병원 2001 仁濟醫學 Vol.22 No.2

Drugs are important causes of rhabdomyolysis. In Korea, however, there is no report of rhabdomyolysis developed by methamphetamine intoxication. We experienced a case of rhabdomyolysis developed in a methamphetamine poisoning patient. The patient was a 25-year-old man presenting with altered mental status. Results of further investigations showed evidence of acute renal failure and rhabdomyolysis. He was treated with general supportive care including hydration, urine alkalization and hemodialysis, but this patient died eventually. This case shows the importance of the suspiction of illicit drugs including methamphetamine as a cause of rhabdomyolysis with an unclear etiology.

알콜성 간염을 동반한 만성 음주자에 병발된 횡문근융해증 및 급성신부전증 3예

김희승,문봄내,김태균,윤기현,최동진,한상훤,박찬호,김민호,이성주,김영훈 인제대학교 백병원 2001 仁濟醫學 Vol.22 No.2

Acute alcoholic myopathy may present as a fulminant syndrome of muscular pain, swelling and tenderness in extremities associated with rhabdomyolysis and acute renal failure. Acute renal failure caused by rhabdomyolysis and myoglobinuria is one of the serious complications of alcohol and may be lethal occasionally. We report 3 cases of acute renal failure caused by rhabdomyolysis and myoglobinuria associated with alcoholic intoxication, treated in Pusan Medical Center between August 1999 and July 2000.

자궁내 피임 장치와 동반된 복부 및 골반 방선균증 1예

최동진,윤기현,김태균,문봄내,박찬호,한상훤,이민재,윤중근,이세용,설상영 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.2

Actinomycosis is an uncommon chronic, suppurative granulomatous disease, caused by an anaerobic bacterium. Actinomyces israeli, which is a component of human oral and gastrointestinal flora. The cervicofacial region is the commonest site of disease, and the abdominopelvic region is the second commonest site. In most cases the diagnosis is made postoperatively because of its unusual clinical presentation. Although the incidence of actinomycosis has decrease, the abdominal-pelvic form has been increasing over the past several years secondary to increased prolonged use of the intrauterine device. We report here the case of a patient with the most severe rare form of abdominal wall and pelvic involvement, characterized by dense adhesions between large and small bowel and large bowel and uterus, adnexa, and bladder associated with intrauterine contraceptive device.