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pBRG-4 를 이용한 Metarhizium anisopliae 의 형질전환
이동규,예완해,황철원,권석태,강선철 ( Dong Gyu Lee,Wan Hae Yeh,Cher Won Hwang,Suk Tae Kwon,Sun Chul Kang ) 한국응용생명화학회 1998 Applied Biological Chemistry (Appl Biol Chem) Vol.41 No.3
We have established a transformation system for entomopathogenic fungus, Metarhizium anisopliae, in order to develop mycoinsecticide by recombinant DNA techniques. Protoplasts of M. anisopliae would be transformed to a benomyl-resistant by introducing pBRG-4 plasmid DNA, which contains a β-tubulin gene of Aspergillus flavus conferring resistance to benomyl and a pyr4 gene of Neurospora crassa, in the presence of 5% polyethylene glycol and 10 mM calcium chloride. Transformants occuring at a frequency of 10 colonies per 50 ㎍ pBRG-4 DNA grew on the 5 ㎍/㎖ concentrations, of benamyl, while the wild type was inhibited by 2.5 ㎍/㎖. From the Southern analysis using genomic DNAs isolated from M. anisopliae transformants, the positive signals suggested that the β-tubulin gene had integrated in the M. anisopliae genome by homologous recombination.
이동규,손승원,오창석 ( Dong Kyu Lee,Sung Won Sohn,Chang Suk Oh ) 충북대학교 산업과학기술연구소 1996 산업과학기술연구 논문집 Vol.10 No.2
Abstract_Roman In this paper, the adaptive prefiltering method to compensate CDV for VBR service in AAL5 layer is proposed. To transfer the isochronous video data in ATM network, there are many important factors to be considered, such as selection of AAL
이동규,김동식,김재설 ( Dong Kyu Lee,Dong Sik Kim,Jae Surl Kim ) 충북대학교 산업과학기술연구소 1996 산업과학기술연구 논문집 Vol.10 No.2
Abstract_Roman Low temperature effluent gases from combustion systems are generally hard to recover economicaUy since the sour gases(Nox and Sox) contained in the flue gas are condensed during the cooling process and thus produce the sour solution which
이동규,김해봉,양병도,김영석,이형규,Lee, Dong-Kyu,Jin, Hai-Feng,Yang, Byung-Do,Kim, Young-Suk,Lee, Hyung-Gyoo 한국전기전자재료학회 2010 전기전자재료학회논문지 Vol.23 No.8
The devices based on electrically erasable programmable read-only memory (EEPROM) structure are proposed for the detection of external electric charges. A large size charge contact window (CCW) extended from the floating gate is employed to immobilize external charges, and a control gate with stacked metal-insulator-metal (MIM) capacitor is adapted for a standard single polysilicon CMOS process. When positive voltage is applied to the capacitor of CCW of an n-channel EEPROM, the drain current increases due to the negative shift of its threshold voltage. Also when a pre-charged external capacitor is directly connected to the floating gate metal of CCW, the positive charges of the external capacitor make the drain current increase for n-channel, whereas the negative charges cause it to decrease. For an p-channel, however, the opposite behaviors are observed by the external voltage and charges. With the attachment of external charges to the CCW of EEPROM inverter, the characteristic inverter voltage behavior shifts from the reference curve dependent on external charge polarity. Therefore, we have demonstrated that the EEPROM inverter is capable of detecting external immobilized charges on the floating gate. and these devices are applicable to sensing the pH's or biomolecular reactions.
방향성 결합기 및 핀 다이오드 스위치를 이용한 10 비트 임피던스 튜너
이동규,이상효,권영우,Lee, Dong-Kyu,Lee, Sang-Hyo,Kwon, Young-Woo 한국전자파학회 2007 한국전자파학회논문지 Vol.18 No.7
본 논문에서는 방향성 결합기를 이용한 새로운 형태의 임피던스 튜너를 제안하였다. 이 튜너를 signal flow graph를 이용하여 해석하였고, 이 방식은 직렬 라인의 길이 변화가 어려운 single stub 방식에 비해 구현이 용이하고 double stub 방식에 비해 넓은 튜닝 범위를 가진다. 10개의 핀 다이오드를 이용하여 제작된 튜너는 10개의 스위치가 비트 형태로 동작하면서 Smith 차트 전 영역에서 $2^{10}$개의 균일한 임피던스 분포를 가지도록 설계되었다. 제작된 튜너는 $1.8{\sim}2.2GHz$의 대역폭에서 넓은 튜닝 영역을 가지며, 반사 계수 크기는 최대 0.9까지 측정되었다. 측정된 임피던스 값을 바탕으로 load-pull 시스템을 구성하였고, 전력 소자용 트랜지스터의 최적 출력단 임피던스를 찾아내었다. In this paper, a novel impedance tuner using a directional coupler is proposed. The design topology is analyzed by signal flow graph(SFG) and shows advantages compared with conventional single and double stub methods from the view points of easy implementation and wide tuning range, respectively. This impedance tuner consists of ten switches and its $2^{10}$ tuning points are distributed uniformly on the whole Smith chart. The measured maximum magnitude of the reflection coefficient is 0.9. And the fabricated impedance tuner has a wide bandwidth from 1.8 to 2.2 GHz. Using this impedance tuner, we did a load-pull measurement of a power transistor.
수중에서 고유수용성신경근촉진법 하지 패턴이 근력 및 유연성에 미치는 영향
이동규,김용남,Lee, Dong-Kyu,Kim, Yong-Nam 대한물리치료학회 2013 대한물리치료학회지 Vol.25 No.2
Purpose: This study was conducted in order to analyze the effect of proprioceptive neuromuscular facilitation lower extremity pattern on muscular strength and flexibility in an aquatic environment. Methods: Ten members of the experimental group and 10 members of the control group were randomly selected from 20 college students who are in their 20s. Bilateral symmetry pattern among proprioceptive neuromuscular facilitation lower extremity patterns was applied in an aquatic environment in the experimental group three times per week for a period of six weeks, and a set consisted of 10 times, which was repeated 10 times. On the other hand, subjects in the control group did not receive any treatment while maintaining daily life. Muscular strength was measured using a dynamometer and electromyogram system for maximum voluntary isometric contraction of hip flexor, hip extensor, knee flexor, and knee extensor. Cervical flexibility was measured using cervical range of motion instrument for cervical flexion, rotation, and lateral flexion. Lumbar flexibility was measured using back range of motion instrument II for lumbar flexion. Results: For the experimental group, significance was observed for hip flexor, extensor, knee flexor, extensor, cervical flexion, rotation, lateral flexion, and lumbar flexion. For the control group, no significance was observed for any other variables except for cervical flexion. As a result of observation of difference between the two groups, relatively high significance was observed in the experimental group, compared with the control group. Conclusion: In conclusion, proprioceptive neuromuscular facilitation lower extremity pattern had a positive effect on muscular strength and flexibility in an aquatic environment.