항MRSA 활성을 보이는 Paenibacillus incheonensis YK5의 분리 및 특성

윤영준,김혜영,이태수,김정완,Yoon, Young-Jun,Kim, Hye-Yoong,Lee, Tae-Soo,Kim, Jung-Wan 한국미생물학회 2008 미생물학회지 Vol.44 No.4

국내 토양으로부터 MRSA에 대해 항균활성을 보이는 세균들을 분리하였다. 이들 가운데 가장 높은 항MRSA 균 활성을 보인 YK5 균주는 호기성 간균으로 운동성이 있고, 내생포자를 형성하였으며, 인돌을 형성하지 않았고, mannitol을 분해하여 산을 생성시키지 않았다. 이 균주의 16S rRNA 유전자 염기서열은 Paenibacillus 속 세균들의 유전자와 $95{\sim}98%$의 상동성을 나타냈는데 특히 P. elgii와 가장 높은 상동성을 보였으나, 기타 생리 생화학적 특성에서 다른 점들이 관찰되어 P. incheonensis YK5로 명명되었다. P. incheonensis YK5 를 SST 배지에 접종하여 $37^{\circ}C$에서 96 시간 배양한 다음 그 상등액으로 부터 부탄올로 추출된 항균물질은 그람 양성균인 MRSA 20 균주들과 Streptococcus pneumoniae, 그람 음성균인 Pseudomonas aeruginosa 10균주들, Salmonella spp., Shigella spp., Escherichia coli와 Klebsiella pneumoniae 및 인체병원성 진균인 Cryptococcus neoformans와 Trichophyton spp.에 대하여 광범위한 항균효과를 나타냈다. 또한 이 항균물질은 $20{\sim}100^{\circ}C$ 사이의 온도구간에서 안정한 활성을 유지하였을 뿐만 아니라, pH $3.0{\sim}7.0$의 넓은 pH범위에서 활성을 나타내 MRSA를 비롯한 다양한 병원균에 대한 항생물질로서의 가능성을 보였다. Various bacteria were isolated from Korean soil samples based on their capability inhibiting the growth of MRSA strains. Among them, strain YK5 with the highest activity was a Gram positive sporulative bacillus with motility. It did not produce indole and no acid was formed from mannitol by the bacterium. The 16S rRNA sequence of the strain showed $95{\sim}98%$ homology with those of Paenibacillus spp.. The bacterial isolate shared the highest homology with that of P. elgii (98%), but was named as Paenibacillus incheonensis YK5 due to differences in physiological properties. Butanol extract of the P. incheonensis YK5 culture grown in SST medium at $37^{\circ}C$ for 96 hr showed a broad antimicrobial activity against Gram-positive (MRSA and Streptococcus pneumoniae) and negative (Pseudomonas aeruginosa, Salmonella spp., Shigella spp., Escherichia coli, Klebsiella pneumoniae) pathogenic bacteria and fungi (Cryptococcus neoformans and Trichophyton). The antimicrobial activity in the crude extract was stable in a broad range of temperature and pH, $20{\sim}100^{\circ}C$ and $3.0{\sim}6.0$, respectively. Therefore, the antimicrobial activity of P. incheonesis YK5 had potential as a novel antibiotics for pathogens including MRSA.

멸종위기 야생생물 I 급 털복주머니란 서식지 복원을 위한 서식지 적합성 지수(HSI) 개발

윤영준,김선령,장래하,한승현,이동진,심윤진,박용수,Yoon, Young-Jun,Kim, Sun-Ryoung,Jang, Rae-Ha,Han, Seung-Hyun,Lee, Dong-Jin,Shim, Yun-Jin,Park, Yong-Su 한국환경복원기술학회 2020 한국환경복원기술학회지 Vol.23 No.4

This study aimed to develop the HSI (Habitat Suitability Index) model of Cypripedium guttatum. and to verify this model by applying to the candidate sites for replacement habitat. The development of HSI and SI (Suitability Index) model was conducted based on the existing literature, field surveys, and expert opinions for information on ecological habitat characteristics. Seven variables were selected as habitat variables including mean maximum temperature in Jul.-Aug., lighting, slope, altitude, effective soil depth, soil texture, and artificial overexploitation (i.e. protected areas). HSI model was developed for C. guttaum based on these variables. This HSI model showed high applicability to selection and evaluation of replacement habitats for C. guttaum. Our findings could provide the basic information on habitat assessment to prevent the extinction of endangered C. guttatum. However, since there is a limitation that the survey data were insufficient, further field surveys should be conducted on several habitat types to improve the accuracy of the HSI model.

복합재료역학을 이용한 콜라겐 단백질 마이크로피브릴의 탄성율 예측 개선

윤영준,배철수,Yoon, Young-June,Bae, Cheol-Soo 대한의용생체공학회 2010 의공학회지 Vol.31 No.3

The effective Young’s modulus of a microfibril surrounded by water may be simply calculated by using the upper (Voigt) and lower (Reuss) bounds, which is one way to estimate the Young’s modulus in composite materials. The Steered Molecular Dynamics (SMD) has been used for estimating the Young’s modulus of a microfibril surrounded by water. In this paper, the result estimated by the upper (Voigt) and lower (Reuss) bounds shows 9.2% to 21.8% discrepancy from the result estimated by SMD, but introducing “efficiency of reinforcement parameter” removes the discrepancy and shows good agreement with the result estimated by SMD. We found the best fit for the Young’s modulus against the size of the gap between microfibrils. Also the steps using these bounds are much simpler than SMD.

한양방 치료를 병행한 남성형 탈모 환자 4례 증례 보고

윤영준,권나현,신현진,장진영,Yoon, Young-Joon,Kwon, Na-Hyun,Shin, Hyun-Jin,Jang, Jin-Young 대한한방안이비인후피부과학회 2017 한방안이비인후피부과학회지 Vol.30 No.2

Objectives : The purpose of this study is to report the effectiveness of the combination of herbal medicine and western medicine in androgenetic alopecia patients. Methods : This case study was conducted for four androgenetic alopecia patients who have visited Korean medicine clinic. Gagamcheongyoung-tang($Ji{\bar{a}}ji{\check{a}}nq{\bar{i}}ngy{\acute{i}}ng-t{\bar{a}}ng$) was prescribed to all four patients who were already taking finasteride over 1 year. Improvements of patients were evaluated through photographs. Results : As a result of examining photographs, symptoms of alopecia in four cases were improved. Conclusions : Gagamcheongyoung-tang($Ji{\bar{a}}ji{\check{a}}nq\;{\bar{i}}ngy{\acute{i}}ng-t{\bar{a}}ng$) has advantages of treating a androgenetic alopecia patients who are taking finasteride longer than 1 year.

알긴산 분해능을 갖는 Pseudoalteromonas 및 Vibrio 속 해양세균들의 분리 및 특성분석

윤영준,김정완,Yoon, Young-Jun,Kim, Jung-Wan 한국미생물학회 2015 미생물학회지 Vol.51 No.4

알긴산의 응용을 위하여 인천지역에서 수집한 다양한 패류와 해수로부터 알긴산 분해효소 활성이 우수한 103개의 균주를 분리하고 그 중 M1-2-1, M6-1, C8-15 등 분해능이 가장 우수한 3균주를 선발하여 그 특성을 분석하였다. 이들은 모두 그람 음성 간균이었고, 운동성이 있는 호염성 세균이었다. 또한 생리 생화학적 특성 분석과 16S rRNA 유전자의 염기서열 분석으로 M1-2-1과 M6-1은 Pseudoalteromonas 속, C8-15은 Vibrio 속에 속하는 세균으로 동정되었다. 이들의 알긴산 분해효소 활성은 알긴산이 유일한 탄소원인 APY 배지에 접종하여 $25^{\circ}C$에서 6-8시간 배양했을 때 최대로 나타났고, NaCl을 가했을 때 생장 및 효소 활성 모두 증진되었다. 이 분리 균주들의 알긴산 분해 조효소들은 $45^{\circ}C$와 pH 7.0-8.0에서 가장 높은 활성을 나타냈으며, Pseudoalteromonas sp. M1-2-1과 M6-1 균주는 각각 2.7232 g/L와 1.976 g/L의 환원당 생성능을 보여, 산업적으로 활용 가능성이 높은 것으로 사료되었다. As an effort to utilize alginate, 103 bacterial isolates that were positive for the alginate lyase activity were isolated from various clams and seawater samples collected in Incheon coastal area. Among them, 3 strains (M1-2-1, M6-1, and C8-15) were finally selected for further analysis based on their activities at higher levels than others. These isolates were all Gram-negative and rod shaped halophilic bacteria with motility. According to their physiological and biochemical properties as well as DNA sequence of their 16S rRNA genes, M1-2-1 and M6-1 were identified as a member of genus Pseudoalteromonas and C8-15 belonged to genus Vibrio. They exhibited the alginate degrading activity at the maximal level when they were cultured in APY broth for 6-8 h at $25^{\circ}C$. Both their growth and the enzyme activity were greatly enhanced when NaCl was added to the growth medium. The crude alginate lyases from the supernatants of the bacterial cultures showed the highest activity at $45^{\circ}C$ and pH 7.0-8.0. M1-2-1 and M6-1 produced 2.723 and 1.976 g/L of reducing sugar from alginate, respectively, suggesting that they have potential for commercial application.

압력에 따른 맥파의 푸리에 분석

윤영준,이명화,신학수,정현민,조정현,소광섭,Yoon Young-Jun,Lee Myeong-Hwa,Shin Hak-Soo,Johng Hyun-Min,Cho Johng-Hyun,Soh Kwang-Sup 대한예방한의학회 1999 대한예방한의학회지 Vol.3 No.1

압력을 증가시켜가며 맥파를 측정하여 맥파의 진동수와 푸리에 진폭이 가해준 압력에 따라 어떠한 양상을 보이는지 조사하였다. 맥파의 기본 진동수는 압력에 따른 변화를 거의 보이지 않았으며, 푸리에 진폭은 개인의 체질에 따라 몇 가지 유형으로 분류될 수 있음을 보였다. 이 유형들을 다시 푸리에 진폭이 최고점에 이를 때의 압력의 크기와 최고점에서의 상태를 유지할 수 있는 압력의 범위에 따라 맥상의 여러 형태와 연관지을 수 있음을 보였다. We investigate how the frequency and the Fourier amplitude of the pulse wave behave as the contact pressure of the sensor on the skin increases. The results are that the fundamental frequency of the pulse wave hardly changes and the Fourier amplitude has several different patterns depending on the personal constitution, and its patterns are classified. We also show that the classified patterns can be related to the different pulse types by the magnitude of the pressure required to reach the maximum Fourier amplitude and the range of the pressure at which the maximum Fourier amplitude is maintained.

간세포암과 감별을 요하는 간결절 간경변에서 발생한 국소결절증식양 결절

윤영준 ( Young Joon Yoon ),윤기태 ( Ki Tae Yoon ),박준용 ( Jun Yong Park ),이현웅 ( Hyun Woong Lee ),김화숙 ( Hwa Sook Kim ),김자경 ( Ja Kyung Kim ),박영년 ( Young Nyun Park ),한광협 ( Kwang Hyub Han ),전재윤 ( Chae Yoon Chon ) 대한간암학회 2007 대한간암학회지 Vol.7 No.-

국제보험회계기준 도입에 따른 책임준비금 평가에 관한 연구 -생명보험회사를 중심으로-

윤영준 ( Yeong Jun Yoon ) 한국계리학회 2011 계리학연구 Vol.3 No.1

국제회계기준위원회는 국제적으로 통일된 회계기준을 정하기 위해 국제회계기준을 제정·발표하였으며, 우리나라 또한 201ㄷ1년부터 국제회계기준을 전면 도입하여 모든 상장사 및 금융기관에 적용할 예정이다. 이에, 본 논문에서는 책임준비금과 국제회계기준의 국내 도입시의 영향 및 대응방안 등에 대하여 검토하였으며, 현재 국제회계기준위원회가 2단계로 검토하고 있는 보험부채의 공정가치 평가방안의 주요 내용(Discussion Paper)을 기반으로 사례분석을 실시하였다. 사례분석은 실제 보험회사의 표준계약(보장성보험)을 대상으로 장래요구자본의 대용치로 보험금을 사용하여 시장가치마진을 계산한 후 책임준비금 공정가치 산출금액과 현재의 법정준비금을 비교하였으며, 각 기초율별 민감도 테스트를 실시하였다. International Accounting Standard Board(IASB) has issued International Financial Reporting Standards(IFRS) in order to improve and converge financial reporting standards. Korea has also scheduled to fully introduce IFRS in 2011 for all listed companies and financial institutions. Accordingly, this thesis analyzed Life Insurance reserve and IFRS and studied how IFRS4 effects on the local insurance accounting systems and how local companies correspond to IFRS4. And then, The case analysis discussed valuation models using fair value measurement which was discussed as a main issue of IFRS Phase Ⅱ. The models are tested for standard real contracts(specifically, non-deposit type), are compared with the current reserve amount. The models include sensitivity tests on basic assumptions such as discount rate, surrender rate, etc. As a result, the amount of reserve measured by fair value assumptions is less than that of current legal reserve, and rate of claim payment is the most sensitive regarding reserve volatility. The other side, surrender rate doesn`t have a big impact on amount of fair valued reserve.

망상골에서의 골조직의 재생성 예측

윤영준(Young June Yoon) 대한기계학회 2022 대한기계학회 춘추학술대회 Vol.2022 No.11

망상골에서의 골조직의 재생성 예측

윤영준(Young June Yoon) 대한기계학회 2022 대한기계학회 춘추학술대회 Vol.2022 No.11