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CD40L 발현 K562 세포주를 이용한 시험관내 B 세포 증식과 항원제공능 증가
박정용,윤성희,김은경,윤선옥,손현정,김태규,Park, Jung-Yong,Yoon, Sung-Hee,Kim, Eun-Kyung,Yun, Sun-Ok,Sohn, Hyun-Jung,Kim, Tai-Gyu 대한면역학회 2007 Immune Network Vol.7 No.2
Background: CD40-activated B (CD40-B) cells might be an attractive source of autologous antigen-presenting cells (APCs) for immunotherapy due to the convenience to obtain from peripheral blood and expand in vitro. Moreover, CD40-B cells were found to be comparable with DCs in their capacity to raise antigen-specific CD8+ T cells. Here, we have established K562 cells expressing CD40L to expand CD40-activated B cells used for APCs. Methods: After activation of B cell by K562/CD40L, CD40-B cells were examined by counting B cell numbers. Surface expression of CD54, CD80, CD86 and HLA class II was measured by flow cytometry. The CD40-B cells were tested for its function as APC by mixed lymphocyte reactions (MLR) and by induction of T cell responses specific for pp65 peptide in vitro. Results: The expansion of B cells by K562/CD40L increased about 6-folds compared with anti-CD40 or K562. Furthermore, the expression of CD54, CD80, CD86 and HLA class II was up-regulated by K562/CD40L. B cells by K562/CD40L showed comparable antigen presentation activity with mature DCs as shown in MLR, INF-${\gamma}$ ELISPOT assay. Conclusion: These results suggest that K562/CD40L could be used to generate activated B cells as potent APCs which could be useful for cellular vaccination and adoptive immunotherapy.
임신 중 난소갑상선종에서 기원하여 복막 파종된 형태로 진단된 여포상암종
김영태 ( Young Tae Kim ),김재훈 ( Jae Hoon Kim ),윤선옥 ( Sun Ok Yoon ),이사라 ( Sa Rah Lee ),최보람 ( Bo Ram Choi ),김보욱 ( Bo Wook Kim ) 대한산부인과학회 2012 Obstetrics & Gynecology Science Vol.55 No.12
Struma ovarii is defined as a rare monodermal form of ovarian teratoma that occupies more than 50% of thyroid tissue. Only 2% of struma ovarii display malignant histology, most of which is revealed as papillary carcinoma type. Follicular carcinoma originating from malignant struma ovarii is rarely reported. We report a case of a pregnant woman diagnosed with peritoneal dissemination of follicular carcinoma arising from struma ovarii.