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- 저자 : 유대성(Yoo Dae Sung) (검색결과 15 건)
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Antiaging Effects of Musa sapientum L. (Banana) Leaf Extract
유대성,장수진,박윤정,김승회,황형서,Yoo, Dae Sung,Jang, Su Jin,Park, Yun Jeong,Kim, Seung Hoi,Hwang, Hyung-seo The Korean Society for Biotechnology and Bioengine 2016 KSBB Journal Vol.6 No.2
To examine the possibility of using Musa sapientum L. (Banana) leaf extract as a cosmetic raw material, banana leaves grown in Jeju Island were extracted with 70% ethanol. Polysaccharides present in banana leaf extract were discarded by precipitation with cold ethanol. Polysaccharide-discarded banana leaf extract promoted procollagen and COL1A1 gene expression, but inhibited matrix metalloproteinase (MMP)-1 and MMP-2 gene expression in human skin fibroblasts when examined by real-time reverse-transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). The active compound in banana leaves was identified by fractionation with various solvents. The chloroform fraction showed the highest anti-wrinkle efficacy and the active compound of chloroform fraction was identified as corosolic acid by NMR, FT-IR, EA, and HPLC-MS. In addition, banana leaf extract showed anti-oxidative efficacy with an IC50 value of 67.91 ppm, as determined by DPPH free radical scavenging assay. Finally, the anti-wrinkle efficacy of banana leaf extract-containing cream was confirmed by clinical tests. Based on these results, banana leaves could have an application as a cosmetic raw material with anti-wrinkle efficacy.
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유대성(Yoo Dae-Sung),박원주(Park Won-Ju),김선영(Kim Sun-Young),서동일(Seo Dong-II),오창석(Oh Chang-Suk) 한국콘텐츠학회 2003 한국콘텐츠학회 종합학술대회 논문집 Vol.1 No.2
최근의 해킹 동향은 네트워크 상의 대역폭을 목표로 하는 트래픽 폭주 공격이 증가하고 있는 추세이다. 반면 이러한 위협으로부터 네트워크 내의 공격 트래픽을 추출하는 기술은 아직 부족한 실정이다. 이에 본 논문에서는 네트워크 서비스 제공을 위협하는 트래픽을 추출하는 방법을 제시하고, DDoS 공격을 효과적으로 탐지하는 알고리즘을 제안하였다. The recent hacking trend is a traffic flooding attack against a bandwidth in the network grows more and more. On the other hand, technology, which extracts attack traffic in the network from these threats, is still short. Therefore, we propose methodology which can measure traffic that threaten network services, and algorithm which can detect DDoS attacks effectively.
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유대성(Yoo Dae-sung),구향옥(Koo Hyang-Ohk),오창석(Oh Chang-suk) 한국콘텐츠학회 2004 한국콘텐츠학회 종합학술대회 논문집 Vol.2 No.2
네트워크망의 급속한 발전 속에서 해커에 의한 많은 피해 사례가 증가되고 있다. 최근에는 트래픽 폭주 공격에 의해 많은 네트워크와 시스템 자원들이 피해를 보고 있다. 이에 따라 네트워크상에서 유통되는 트래픽 분석을 통한 자원의 보호가 중요한 이슈로 대두되고 있다. 이에 본 논문에서는 기존의 SNMP를 이용한 트래픽 분석 방법에 있어서 시간과 탐지력을 향상시킨 알고리즘을 제안하고 구현하였다. A rapid development of the network brought increasing of many damage cases by hacker's attack. In recently many network and system resources are damaged by traffic flooding attacks. For this reason, the protection of network resources by analyzing traffic on the network is on the rise. In this paper, algorithm that improves the executing time and detection rate than traffic analysis method using SNMP is proposed and implemented.
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Antiaging Effects of Musa sapientum L. (Banana) Leaf Extract
Dae Sung Yoo(유대성),Su Jin Jang(장수진),Yun Jeong Park(박윤정),Seung Hoi Kim(김승회),Hyung-seo Hwang(황형서) 한국생물공학회 2016 KSBB Journal Vol.31 No.2
To examine the possibility of using Musa sapientum L. (Banana) leaf extract as a cosmetic raw material, banana leaves grown in Jeju Island were extracted with 70% ethanol. Polysaccharides present in banana leaf extract were discarded by precipitation with cold ethanol. Polysaccharidediscarded banana leaf extract promoted procollagen and COL1A1 gene expression, but inhibited matrix metalloproteinase (MMP)-1 and MMP-2 gene expression in human skin fibroblasts when examined by real-time reverse-transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). The active compound in banana leaves was identified by fractionation with various solvents. The chloroform fraction showed the highest anti-wrinkle efficacy and the active compound of chloroform fraction was identified as corosolic acid by NMR, FT-IR, EA, and HPLCMS. In addition, banana leaf extract showed anti-oxidative efficacy with an IC50 value of 67.91 ppm, as determined by DPPH free radical scavenging assay. Finally, the anti-wrinkle efficacy of banana leaf extract-containing cream was confirmed by clinical tests. Based on these results, banana leaves could have an application as a cosmetic raw material with anti-wrinkle efficacy.
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황형서 ( Hyung Seo Hwang ),유대성 ( Dae Sung Yoo ),심중현 ( Joong Hyun Shim ) 대한화장품학회 2016 대한화장품학회지 Vol.42 No.1
바나나잎의 기능성화장품 소재로써 응용 가능성을 규명하고자 바나나잎 추출물이 피부 미백에 미치는 효과를 B16F10 세포주를 활용하여 cell viability, mRNA 발현 및 tyrosinase (Tyr) 활성 저해 실험을 통하여 알아보았다. 10%의 바나나잎 추출물은 tyrosinase 활성을 저해하고, 멜라닌 합성을 65% 감소시키는 것을 확인하였다. 정량적 real-time RT-PCR을 수행한 결과 바나나잎 추출물이 tyrosinase related protein 1 (TRP1)/ tyrosinase related protein 2 (TRP2) / tyrosinase mRNA 발현을 각각 20 / 40 / 60% 가량 억제하는 효과를 확인하였다. 또한 바나나잎 추출물이 함유된 크림제형의 임상시험을 수행하여 자외선 조사 직후 바나나잎 추출물이 함유된 크림의 육안평가 결과 유의한 미백효과가 있는 것을 확인하였다. 본 연구 결과는 바나나잎 추출물이 미백 기능성 화장품 소재 뿐 아니라 향후 기전 연구 수행을 통해 다양한 산업화 소재로 개발될 수 있음을 시사한다. This research was carried out to identify the whitening effect of Banana leaf extract. B16F10 cells were used to measure cell viability, mRNA expression, and tyrosinase activity inhibition assay from B16F10 cell. We also carried out clinical test of the cream product containing banana leaf extract. In this study, we elucidated the effects of banana leaf extract on TRP1 / TRP2 / Tyr mRNA expression and tyrosinase activity inhibition. Quantitative real-time PCR showed that banana leaf extract decreased mRNA level of TRP1, TRP2 and Tyr gene and tyrosinase activity inhibition assay also revealed that banana leaf extract 65% decreased melanin production in B16F10 cell. Banana leaf extract cream can whiten the skin darkness induced by ultraviolet. Therefore, we successfully identified the whitening effect of banana leaf extract, and this finding suggested the banana leaf extract is a considerable potent cosmetic ingredient for skin whitening. Based on this, we anticipated further researches about banana leaf extract for mechanism to develop not only cosmetics but healthcare food or medicines.
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박한상(Park han-sang),유대성(Yoo dae-sung),오창석(Oh chang-suk) 한국콘텐츠학회 2004 한국콘텐츠학회 종합학술대회 논문집 Vol.2 No.1
최근의 트래픽 폭주 공격은 웜의 확산성과 공격 방법의 발달로 인하여 공격 전개시간이 단축되었다. 하지만 기존의 트래픽 분석 방법에서는 최근의 트래픽 폭주 공격의 빠른 진행을 탐지하기에는 시간의 제약점이 있다. 본 논문에서는 이러한 취약점을 보완하기 위해 트래픽을 수집하고 분석하는데 있어 시간을 향상시킨 트래픽 수집 및 분석 알고리즘을 제안하고 구현하였다. Recently traffic flooding attack has happened faster and faster owing to expansion of the worm attack and development of the method of traffic flooding attack. The method in the past time is problematic in detecting the recent traffic flooding attacks, which are running quickly. Therefore, this paper aims to establish the algorithm which reduces the time of detection to traffic flooding attack in collecting and analyzing traffics.
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전지민 ( Ji Min Jeon ),유대성 ( Dae Sung Yoo ),천종우 ( Jong Woo Cheon ),권순식 ( Soon Sik Kwon ),전소하 ( So Ha Jeon ),박수남 ( Soo Nam Park ) 대한화장품학회 2014 대한화장품학회지 Vol.40 No.1
본 연구에서는 추출 온도에 따른 금불초 꽃(I. britannica var. chinensis) 추출물의 항노화 효능을 알아 보았다. 추출 온도에 따른 세포독성을 살펴본 결과, 상온, 45 ℃와 65 ℃ 추출물 모두 0.1% 이하의 농도에서 세포독성이 관찰되지 않았다. 0.1% 이하의 농도에서 항노화 효능평가를 수행한 결과, 0.1% 농도의 상온 추출물 경우에 멜라닌 생성 억제율은 24.5%로 45 ℃와 65 ℃ 추출물과 비교하여 멜라닌 생성 억제율이 큰 것으로 나타났다. 0.1% 농도의 상온 추출물을 처리한 세포의 히알루론산 합성 관련 유전자 HAS-1, HAS-2 및 HAS-3의 발현율은 각각 123.3%, 137.8% 및 133.2%를 나타냈으며, 이는 비교물질인 L-ascorbic acid (115.6%, 121.0% 및 131.4%) 경우 보다 약간 크게 나타났다. 금불초 꽃 45 ℃ 추출물의 경우, 0.1%의 농도에서 염증유발 유전자인 TNF-α, COX-2 및 IL-1α의 발현율이 대조군 대비 각각 30.3%, 12.8%, 25.7%로 감소하였으며, 이는 상온, 65 ℃ 추출물과 비교하여 염증유발 유전자 발현 억제 효과가 더 큰 것으로 나타났다. 이상의 결과들로 볼 때, 금불초 꽃 추출물은 추출 온도에 따라 멜라닌 생성 억제, 히알루론산 합성 관련 유전자 발현 증가 효과, 염증유발 유전자 발현 억제 효과를 나타내며, 그로 인하여 피부에서 미백, 보습, 항염 효능을 통해 피부 항노화 효능을 가질 것으로 사료된다. 이는 금불초 추출물이 항노화 화장품 원료로서 응용 가능성이 있음을 시사한다. In this study, the extracts of Inula britannica var. chinensis (I. britannica) flower were extracted at three different temperatures (room temperature, 45 ℃, and 65 ℃) and their anti-aging effects were studied. Before investigating anti-aging effects of the extracts, their cytotoxicity was tested on B16F10, Hs683, and HaCaT cells. All extracts showed no cytotoxicity at the concentration less than 0.1% (v/v). Melanin synthesis inhibitory activities in B16F10 cells and reverse transcriptase polymerase chain reaction (RT-PCR) in Hs683 and HaCaT cells were used to see their anti-aging effects. The room temperature extract at 0.1% showed 24.5% melanin synthesis inhibition, which was better than the 45 ℃ and 65 ℃ extracts. In addition, expression rates of the room temperature extract at 0.1% on HAS-1, HAS-2, and HAS-3 related to hyaluronan synthase genes were 123.3%, 137.8%, 133.2%, respectively. which were higher than reference material of L-ascorbic acid. Expression rates of the 45 ℃ extract at 0.1% on TNF-α, COX-2, and IL-1α, which are inflammatory related genes, was suppressed to 30.3%, 12.8%, 25.7%, respectively. It was better in anti-in flammatory effect than the room temperature and 65 ℃ extracts. As results, we showed that I. britannica var. chinensis flower extarcts decreased melanin production and expression of inflammatory related genes and increased the expression rate of hyaluronan synthase genes. Thus, it is believed that the extracts affect anti-aging effects of skin through whitening, moisturizing, and anti-inflammatory processes and could be applicable to cosmetics as a functional cosmetic ingredient.
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