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유경만,조남륜,이원희,이영록,박용근 ( Kyung Man You,Nam Ryun Cho,Won Hee Lee,Yung Nok Lee,Yong Keun Park ) 한국환경생물학회 1995 환경생물 : 환경생물학회지 Vol.13 No.2
Twelve mercury-resistant bacterial strains were isolated from Anyangcheon. The mercury-resistant bacteria grown on the LB agar supplemented with 100㎍/㎖ of mercuric chloride were identified as Pseudomonas sp. and Bacillus sp.. All of the mercury-resistant bacteria exhibited resistance to at least one antibiotic. Among them, PY109. PY110, and PY112 were broad-spectrum strains which showed resistance to not only inorganic mercuric compound such as mercuric chloride(HgCl_2). but also organomercurial compound such as phenyl mercuric acetate (PMA). The remaining nine were narrow-spectrum strains resistant only to inorganic mercuric compound. PY102, PY103, and PY113 were resistant to cadmium(Cd^2+) as well as mercury(Hg^2+). Plasmids were detected in all strains of Pseudomonas sp., while all strains of Bacillus sp. except for PY106 had no plasmid. Growth of Pseudomonas sp. PY109 in the presence of mercury started after an extended lag phase. The growth curve of Pseudomonas sp. PY109 dropped to the lowest level as soon as the bacteria came into contact with HgCl_2 and PMA. However, The bacteria preincubated with a low concentration of HgCl_2 were prevented from early death. The decrease of concentration of Hg^2+ in the culture medium during growth of Pseudomonas sp. PY109 was confirmed by the UV-VIS spectrophotometer and the inductively coupled plasma atomic emission spectrophotometer.