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- 저자 : 박용근 ( Kyung Man You (검색결과 3 건)
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유경만,조남륜,이원희,이영록,박용근 ( Kyung Man You,Nam Ryun Cho,Won Hee Lee,Yung Nok Lee,Yong Keun Park ) 한국환경생물학회 1995 환경생물 : 환경생물학회지 Vol.13 No.2
Twelve mercury-resistant bacterial strains were isolated from Anyangcheon. The mercury-resistant bacteria grown on the LB agar supplemented with 100㎍/㎖ of mercuric chloride were identified as Pseudomonas sp. and Bacillus sp.. All of the mercury-resistant bacteria exhibited resistance to at least one antibiotic. Among them, PY109. PY110, and PY112 were broad-spectrum strains which showed resistance to not only inorganic mercuric compound such as mercuric chloride(HgCl_2). but also organomercurial compound such as phenyl mercuric acetate (PMA). The remaining nine were narrow-spectrum strains resistant only to inorganic mercuric compound. PY102, PY103, and PY113 were resistant to cadmium(Cd^2+) as well as mercury(Hg^2+). Plasmids were detected in all strains of Pseudomonas sp., while all strains of Bacillus sp. except for PY106 had no plasmid. Growth of Pseudomonas sp. PY109 in the presence of mercury started after an extended lag phase. The growth curve of Pseudomonas sp. PY109 dropped to the lowest level as soon as the bacteria came into contact with HgCl_2 and PMA. However, The bacteria preincubated with a low concentration of HgCl_2 were prevented from early death. The decrease of concentration of Hg^2+ in the culture medium during growth of Pseudomonas sp. PY109 was confirmed by the UV-VIS spectrophotometer and the inductively coupled plasma atomic emission spectrophotometer.
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Intracellular Cadmium Transport and Change of Fatty Acids by Cadmium Stress in Azomonas agilis PY101
You, Kyung Man,Park, Yong Keun 한국환경생물학회 1998 환경생물 : 환경생물학회지 Vol.16 No.4
Azomonas agilis PY101에서 카드뮴(Cd^2+)의 세포내 수송에 대한 양이온들의 효과와 카드뮴 스트레스에 의한 지방산의 변화를 관찰하였다. A. agilis PY101에 의한 Cd^2+의 수송이 Mn^2+ 또는 Zn^2+의 존재 하에서 약 40∼50% 정도 감소했으나, Co^2+ or Ni^2+의 존재 하에서는 거의 변화가 없었다. 또한 카드뮴에 의한 A. agilis PY101 초기 성장의 억제 효과가 Co^2+ 또는 Ni^2+의 첨가시 회복되지 않았지만, Mn^2+ 또는 Zn^2+의 첨가시에는 현저하게 회복되었다. 이러한 결과는 카드뮴이 A. agilis PY101의 Mn^2+ 또는 Zn^2+의 수송계를 통하여 세포내로 들어간다는 것을 보여준다. 가스 크로마토그래피를 사용하여 카드뮴 스트레스에 대한 A. agilis PY101의 지방산 조성과 함량 변화를 분석한 결과 15:0과 16:0을 제외한 대부분의 포화 지방산들이 카드뮴 스트레스에 의해 증가되었으며, 불포화 지방산들은 18:1 w9c가 증가되었고, anteiso 17:1 w9c는 검출되지 않았다. 그리고 모든 하이드록시 포화 지방산들은 카드뮴 스트레스에 의해 모두 증가하였으나, 사이클로프로판 포화 지방산들은 모두 감소하였다. 이 결과에서, A. agilis PY101은 카드뮴 스트레스에 의한 지방산들의 변화를 통하여 세포막의 견고성을 증가시켜 카드뮴의 투과를 감소시킴으로써 내성을 지니는데 기여할 것으로 사료된다. Azomonas agilis PY101 was tested for the effect of divalent cations on intracellular transport of cadmium, and the change of fatty acids by cadmium stress was also observed. This study showed that Cd^2+ transport was reduced about 40∼50% in the presence of Mn^2+ or Zn^2+ and was not almost effected in the presence of Co^2+ or Ni^2+. Early gorwth of A. agilis PY101 is inhibited by the addition of Cd^2+. Although A. agilis PY101 was not recovered from the growth inhibition effect of Cd^2+ by the addition of Co^2+ or Ni^2+, however the strain was recovered from the inhibitory effect of Cd^2+ by the addition of Mn^2+ or Zn^2+. The changes of cellular fatty acids prepared from the cells cultured with or without cadmium was observed in the profiles of fatty acids. In saturated fatty acids, 10:0, 12:0, 14:0, 17:0, and 18:0 were increased, but 15:0 and 16:0 were decreased by cadmium stress. However, in unsaturated fatty acids, 18:1 w9c was increased, and anteiso 17:1 w9c was not detected. All the hydroxy fatty acids(10:0 3OH, 12:0 2OH, 12:1 3OH, 12:0 3OH, and 16:0 2OH) were increased by cadmium stress, but all the cyclopropane fatty acids(17:0 cyclo and 19:0 w8c) were decreased by cadmium stress. In summed features, summed feature 6 was increased slightly by cadmium stress, but summed feature 4 and summed feature 7 were decreased slightly by cadmium stress. Summed feature 3 was not detected by cadmium stress. In these results, it is showed that the increased the rigidity of cell membrane resulting from changes in fatty acid composition and content, contributes to the cadmium tolerance of A. agilis PY101.
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대전 지역의 공단 폐수에서 분리한 수은과 카드뮴 내성 세균의 분리 및 특성
유경만,전희근,박준봉,이인수,박진숙,하남주,박용근 한국환경과학회 1997 한국환경과학회지 Vol.6 No.3
Mercury- and cadmium-resistant bacteria were isolated from an industrial complex wastewater of Taejon area. All of them were motile, gram negative rods. In the results of physicochemical test and VITEK card test, HMI was identified with Acinetobacter calcoaceticus, CM3 was identified with Comamonas acidovorans, HM2, HM3, CM1, and CM4 were Pseudomonas sp., but HM4 and CM2 were unidentified. They were tested for susceptibility to 14 heavy metals. Mercury-resistant bacteria(HM1, HM2, HM3, and HM4) were sensitive to low concentration(100∼400ppm) of Cd^2+, Co^2+, Zn^2+, and Ni^2+ while cadmium-resistant bacteria(CM1, CM2, CM3, and CM4) showed resistance up to the high concentration(600∼1,200ppm) of these metal ions. As a result of resistance spectrum test of mercury-resistant bacteria, HM1 was broad-spectrum strain, HM2, HM3, and HM4 were narrow-spectrum strains. Transmission electron microscopic examination of cell wall of HM1 culture grown with and without 100ppm of HgCl_2 showed remarkably morphological abnormalities. In the result of atomic absorption spectrometric analysis of cadmium-resistant bacteria grown at 200ppm of CdCl_2 for 6h, all of them accumulated cadmium(14ppm∼57ppm) in cell. In cadmium-resistant bacteria, CM1, CM2, and CM4 were spared from the inhibitory effect of Cd^2+ by the addition of Mn^2+, CM4 were also spared from the inhibitory effect of Cd^2+ by the addition of Mn^2+ as well as Zn^2+.
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