Functional characterization of Clonorchis sinensis choline transporter

원정연,Johnsy Mary Louis,Eui Sun Roh,차석호,한진희 대한기생충학ㆍ열대의학회 2023 The Korean Journal of Parasitology Vol.61 No.4

Clonorchis sinensis is commonly found in East Asian countries. Clonorchiasis is prevalent in these countries and can lead to various clinical symptoms. In this study, we used overlap extension polymerase chain reaction (PCR) and the Xenopus laevis oocyte expression system to isolate a cDNA encoding the choline transporter of C. sinensis (CsChT). We subsequently characterized recombinant CsChT. Expression of CsChT in X. laevis oocytes enabled efficient transport of radiolabeled choline, with no detectable uptake of arginine, α-ketoglutarate, p-aminohippurate, taurocholate, and estrone sulfate. Influx and efflux experiments showed that CsChT-mediated choline uptake was time- and sodium-dependent, with no exchange properties. Concentration-dependent analyses of revealed saturable kinetics consistent with the Michaelis–Menten equation, while nonlinear regression analyses revealed a Km value of 8.3 μM and a Vmax of 61.0 pmol/oocyte/h. These findings contribute to widen our understanding of CsChT transport properties and the cascade of choline metabolisms within C. sinensis.

Ion Beam Assisted Deposition법에 의한 TiN박막 제작 및 특성연구

원정연,김종호,강희재,백창현,방상렬,홍주화,위병용 한국진공학회 1997 한국진공학회 학술발표회초록집 Vol.- No.-

99mTc-Pertechnetate 섭취곡선을 이용한 갑상선 기능판별에 대한 연구

원정연 대한영상의학회 1984 대한영상의학회지 Vol.20 No.3

By using 99mTc-Pertechnetate, we evaluated the thyroid function of 136 persons with uptake slope index (U.S.I) which was calculated by computterized dynamic flow study. Also, we compared our result of U.S.I with those of established 1311-24 hr uptake% in given materials by comparative analysis of their correlation with the hormonal values of T3, T4, Free T4. The results were as follows: 1. The U.S.I of euthyroidismal group and hyperthyrodismal group were 4.87$\pm$2.26, 27.67$\pm$9.56 respectively. The 1311-24 hr uptake% of above groups were 29.22$\pm$10.23, and 71.45$\pm$15.51 So the differentiation of the two groups could be done more easily by using 99mTc-Pertechnetate U.S.I than by using 1311-24 hr uptake %. 2. The correlation rates between 99mTc-Pertechnetate U.S.I and other laboratory hormon levels, T3, T4, Free T4, are almost parallel with those between 1311-24 hr uptake % and the values of T3, T4, Free T4, Also the direct correlation rate between 99mTc-Pertechnetate U.S.I, and 1311-24 hr uptake % was 0.898 So, the method of thyroidal function evaluation by 99mTc-Pertechnetate U.S.I isvery reliable. 3. The 99mTc-Pertechnetate U.S.I is very helpful to evaulate the functions of each lobe respectively in cases of having obviously different radioactivity between both lobes. 4. 99mTc-Pertechnetate used dynamic thyroid function study and scan can be performed during the short time without preparation and especially helpful in debilitating patient and patients and patients under antithyroid drug therapy, who need repetitive follow-up examination.

토양 수분 조건에 따른 블랙쵸크베리 ‘Nero’ 과실의 품질 및 세포 발달 비교

원정연,신현석,오영재,한현대,김금선,오세원,김대일 한국자원식물학회 2017 한국자원식물학회지 Vol.30 No.1

Effects of different soil water conditions on fruit characteristics were investigated in 5-year-old ‘Nero’ black chokeberry trees (Aronia melanocarpa). Three kinds of drought stresses, including low water deficit, severe water deficit, and very severe water deficit, due to decline of soil water decreased the fruit quality of weight of 10 berries, soluble solid content, and anthocyanin, compared with the control (consistent water supply) during the harvest period. After longer drought stress, supply of soil water could induce berry cracking because cell size of epidermis of fruits contracted, whereas cell size of sub-epidermis and flesh expanded. Thus periodic water supply using water supply facility is needed for yield and quality of black chokeberry ‘Nero’ fruits Key words – Aronia melanocarpa, Black chokeberry, Cell enlargement, Drought 토양수분의 변화가 블랙쵸크베리 ‘Nero’의 과실 특성에 미치는 영향을 조사하여 고품질 블랙쵸크베리 재배 연구의 기초자료를 제공하고자 수행하였다. 토양수분 감소로 인한 건조스트레스는 수확기 동안 블랙쵸크베리의 과실의 과중, 당도, 안토시아닌 함량 등의 품질을 저하시켰다. 또한 건조기간이 길수록 이후 토양수분 공급시, 과실 표피 세포 크기는 감소하는 반면 아표피, 과육 세포의 크기가 증가하여 열과를 유발할 수 있다. 따라서 관수시설을 통한 일정 주기의 관수가 블랙쵸크베리 과실 생산량 및 품질 향상을 위해 필요할 것으로 생각된다.

재배지역 및 고도에 따른 블랙초크베리 ‘Nero’의 수체 생육 및 과실 특성

원정연,신현석,오영재,한현대,권의석,김대일 한국자원식물학회 2018 한국자원식물학회지 Vol.31 No.2

The study was performed to investigate basic tree growth and fruit characteristics of ‘Nero’ black chokeberry (Aronia melanocarpa) depending on the different cultivation regions and altitudes in 2014, 2015. Tree growth and change of developmental stages of ‘Nero’ were similar regardless of meteorological environment of the four cultivation regions (Danyang, Okcheon, Yeongdong, and Geumsan) in 2014. Fruit characteristics of ‘Nero’ were significant differences in berry weight and anthocyanin content among the four orchards due to difference in cultivation techniques and relatively wide daily temperature range just before harvest, respectively. Tree growth and change of developmental stages of ‘Nero’ grown at the two orchards with different altitudes appeared to be similar during the successive years 2014 and 2015. Soluble solids content of the berries cultivated at low altitude (117 m) was higher than at high altitude (342 m). Acidity showed an inverse pattern with soluble solids content. Anthocyanin content increased progressively until at the middle of August, 2014-15 and then it decreased. Our results showed that black chokeberry is a species adaptive to the domestic environment as there were no differences in tree growth and change of developmental stages of ‘Nero’. Considering fruit quality of black chokeberry such as soluble solids content, acidity, and anthocyanin content, our results suggest that optimal harvest period of black chokeberry ‘Nero’ is August 8 to 19. 재배지와 고도에 따른 블랙초크베리 ‘Nero’의 생육 시기, 수체 생육 및 과실 특성을 구명하여 육종 및 재배·생리 연구에기초자료를 제공하고자 수행하였다. 2014년 재배지별 블랙초크베리 생육 시기 및 수체 생육은 네 재배 지역(단양, 옥천, 영동, 금산)의 기상 환경에 관계없이 유사했다. 재배지별 과실특성은 십립중과 안토시아닌 함량에서 유의적인 차이가 나타났는데 이는 각각 전정방법과 관수 등의 재배 기술과 수확 전 일교차 급증의 영향으로 추측되었다. 2014년과 2015년 고도별 블랙초크베리 ‘Nero’의 생육 시기 및 수체 생육은 유사하게 나타났지만, 과실 특성은 기온이 높은 저지대(117 m)에서 생산된 과실의 가용성고형물이 고지대(342 m)보다 높았던 반면, 산도는 고지대가 높았다. 안토시아닌 함량은 2014년과 2015년 8월 중순까지 증가하다가 고도별로 차이는 있으나 이후부터 감소하는 경향을 보였다. 재배지와 고도별 블랙초크베리 ‘Nero’의 수체 생육및 생육 시기는 차이가 없어 국내 환경에서 재배가 용이한 것으로 생각되며, 수확 시기별 과실 특성을 고려할 때 단양 지역의 블랙초크베리의 최적 수확 시기는 8월 8-19일로 판단되었다.

한국의 신경병성 동통 환자에 대한 역학조사

원정연,김기석,Won, Jung-Yeon,Kim, Ki-Suk 대한안면통증구강내과학회 2008 Journal of Oral Medicine and Pain Vol.33 No.4

The descriptive epidemiology of specific neuropathic pain disorders has not been well-des-cribed, although the burden of neuropathic pain is well recognized. The true incidence of neuro-pathic pain disorder is unknown, but it is believed to be under diagnosed and treated inade-quately, despite the development of various diagnostic system. The purpose of this study was to report the epidemiology of specific neuropathic pain as managed by all kinds of hospital in Korea. A descriptive analysis of the epidemiology of prevalent trigeminal neuralgia(TN)(n-=77,053 27,6%), atypical facial pain(AFP)(n=12,382 4.4%), glossopharyngeal neuralgia(GN)-(n=1,319 0.5%), post-herpetic neuralgia(PHN)-(n=84,598 30.3%), diabetic neuropathy(DN)-(n=85,989 30.8%), atypical odontalgia(AO)-(n=16,001 5.7%) and glossodynia(GD)(n=2,133 0.8%) and treatment departments and treatment durations from computerized Health Insurance Review and Assessment Service(HIRA) of Korea: January 2003 to December 2005, are reported with rates increasing over time for PHN and DN and decreasing for the other neuropathic pain disorders. Most patients were treated at private clinic record for 57.6-72.8% of patients except OA for 10.3%. The percentage of Dept of dentistry for outpatients was 3.2% for TN, 34.7% for AO and 15.4% for GD. Other neuropathic pain patients visited nearly medical clinic.

Effect of Pulse Energy and Pulse Repetition Rate at the Identical Total Power During Enamel Ablation Using an Er:YAG Laser

원정연,김미은,김기석,Won, Jung-Yeon,Kim, Mee-Eun,Kim, Ki-Suk Korean Academy of Orofacial Pain and Oral Medicine 2006 Journal of Oral Medicine and Pain Vol.31 No.3

The objectives of this study was to investigate the amount of tooth ablation and the change of intrapulpal temperature by Er:YAG laser as it relates to pulse energy and pulse repetition rate at the identical power and, thereby, to reveal which of the two parameters strongly relates with ablation efficiency and intrapulpal temperature. Extracted healthy human molar teeth were sectioned into two pieces and each specimen was irradiated within the combination of pulse energy and pulse repetition time at the same power of 3W; $300mJy{\times}10Hz$ group, $200mJy{\times}15Hz$ group, and $150mJy{\times}20Hz$ group. Each specimen comprised ten tooth specimens. A laser beam with conjunction of a water flow rate of 1.6 ml/min was applied over enamel surfaces of the specimens during 3 seconds and the ablation amount was determined by difference in weight before and after irradiation. To investigate the temperature change in the pulp according to the above groups, another five extracted healthy human molar teeth were prepared. Each tooth was embedded into resin block and the temperature-measuring probes were kept on the irradiated and the opposite walls in the dental pulp during lasing. When the power was kept constant at 3W, ablation amount increased with pulse energy rather than pulse repetition rate (p=0.000). Although intrapulpal temperature increased with pulse repetition rate, there were no significant differences among the groups and between the irradiated and the opposite pulpal walls, except at a condition of $150y{\times}20Hz$ (p=0.033). Conclusively, it is suggested that ablation efficacy is influenced by pulse energy rather than pulse repetition rate.

이익유연화(利益柔軟化)에 관한 실증적(實證的) 연구(硏究)

원정연 한국회계학회 1988 회계학연구 Vol.8 No.1

시각적 공간의 경계와 표현 : 상상력에 의한 서랍공간의 존재론적 특성변화

원정연 덕성여자대학교 대학원 2006 덕성여자대학교 대학원 논문집 Vol.8 No.-

인터넷기업의 웹 트래픽 정보와 기업가치

원정연,김경호 한양대학교 디지털경제연구소 2004 디지털경제연구 Vol.9 No.-