Integrated genetic linkage maps for Korean pears ( Pyrus hybrid) using GBS-based SNPs and SSRs

한현대,오영재,Keumsun Kim,Sewon Oh,Sunheum Cho,김윤경,Daeil Kim 한국원예학회 2019 Horticulture, Environment, and Biotechnology Vol.60 No.5

Integrated linkage maps are a valuable tool for comparative mapping between and within genus and species. Novel singlenucleotidepolymorphism (SNP) linkage maps integrated with simple sequence repeats (SSRs) based on pseudo-chromosomesof the Chinese pear reference genome of ‘Dangshansuli’ ( Pyrus bretschneideri ) were constructed. In total, 4801 qualifi edSNP markers were obtained using a customized pipeline. The consensus map for ‘Whangkeumbae’ and ‘Minibae’ contained321 SNP and 30 SSR markers spanning 1511.1 cM with an average genetic distance of 4.3 cM. A total of 30 SSR markersmade it possible to compare our consensus map to other pear and apple maps. SSR markers originating from pear and applemaps showed high co-linearity. SNPs coordinated with pseudo-chromosomes, provide information on physical length coveragefor 17 corresponding linkage groups, and enable easier genome annotation for genomic regions detected by quantitativetrait loci analysis. Genotyping-by-sequencing-based SNP maps integrated with SSRs in the interspecifi c mapping populationillustrate the genomic structure of Korean pear resources and will be used as our reference maps for tribe Maleae.

Assessment of Genetic Relationships in Korean Bred Pears (Pyrus spp.) Using Simple Sequence Repeat Markers

오영재, 박지영, 신현숙, 한현대, 박준형, 김대일 忠北大學校 農業科學硏究所 2016 農業科學硏究 Vol.32 No.1

Genetic relationships of 34 pear cultivars, including 23 Korean bred cultivars and 11 cultivars used as their breeding parents, was analyzed using 12 SSR markers. The total number of alleles was 178 and the average number of alleles was 14.8. The mean of PIC was 0.839. When genetic relationship was analyzed by the neighbor-joining cluster method, 34 cultivars were classified into a total of 4 groups. Group I consisted of only one cultivar, ‘Waseaka’. Group II consisted of ‘Shinsui’, ‘Kimisukawase’, and ‘Kosui’. Group III consisted of 13 cultivars, and divided into three subgroups. Subgroup III-1 included ‘Soowhangbae’, ‘Geumchonjosaeng’, ‘Danbae’, and ‘Youngsanbae’. Subgroup III-2 consisted of ‘Nijisseiki’, ‘Shinil’, ‘Shinko’, and ‘Josaengwhangkeum’. Subgroup III-3 includes ‘Gamro’, ‘Niitaka’, ‘Hanaruem’, ‘Shincheon’, and ‘Whangkeumbae’. The group IV divided into 6 subgroups. The first subgroup consisted of ‘Manhwang’, ‘Chuwhangbae’, ‘Cheongshilli’, and ‘Imamuraaki’. The second, third, and fourth group consisted of only one cultivar, ‘Sunhwang’, ‘Wonhwang’, and ‘Chojuro’, respectively. The fifth subgroup consisted of ‘Whasanbae’, ‘Manpungbae’, ‘Mihwang’, and ‘Hosui’. The sixth subgroup contained ‘Okusankichi’, ‘Mansoo’, ‘Gamcheonbae’, ‘Minibae’, ‘Jinhwang’, and ‘Noksu’. Applying a minimum of six SSR combinations allowed all Korean bred pear cultivars and their parents to be identified.

종자형성능과 기능성 성분이 높은 대추 유전자원 ‘일본’의 형태적 특성

오하경,오세원,한현대,박희순,이경희,신현만,김대일 한국자원식물학회 2020 한국자원식물학회지 Vol.33 No.4

Morphological characteristics of Ziziphus jujuba accessions, including ‘Ilbon’, were investigated to verify the possibility of ‘Ilbon’ as a breeding material. ‘Ilbon’ has smaller leaves and fruits and a lower total soluble solids content compared to the major Z. jujuba accessions. However, total polyphenol content and antioxidant capacity were highest in ‘Ilbon’ among the Z. jujuba accessions. In addition, ‘Ilbon’ could produce intact seeds and seed productivity of ‘Ilbon’ was highest compared with major Z. jujuba accessions. Principal component analysis and clustering analysis using the morphological characteristics of Z. jujuba accessions have been classified them to geographical origin. In particular, ‘Ilbon’ was clearly distinguished from the major Z. jujuba accessions. Therefore, ‘Ilbon’, which has the highest seed productivity and functional component, will be used as a material for cross breeding and will promote genetic study of Z. jujuba. 본 연구는 충청북도농업기술원 대추연구소에서 보유 중인대추 유전자원 중 형태적 다형성을 나타내는 ‘일본’과 주요 대추유전자원의 형태형질을 조사하고, 대추 육종 소재로서 ‘일본’의가능성을 확인하기 위해 수행되었다. ‘일본’은 주요 대추 유전자원과 비교하여 엽과 과실의 크기가 뚜렷하게 작고 당도가 낮았다. 하지만 과실에 폴리페놀 함량이 가장 높았으며, 항산화능력 또한 가장 높았다. 또한 ‘일본’은 종자형성능이 가장 높았다. 대추 유전자원들의 형태형질을 이용하여 분석한 결과, 지리적 기원에 따라 유전자원들이 분류되었다. 특히 ‘일본’은 한국및 중국 유전자원들과 명확히 구분되었다. 따라서 종자형성능이 우수하고 기능성 성분이 높은 ‘일본’은 추후 대추 유전연구및 교배육종을 통한 품종 육성에 유용하게 이용될 수 있을 것으로 생각된다.

Use of Polyhedral Oligomeric Silsesquioxane as a Cross-Linking Reagents in Organic-Inorganic Hybrid Photopolymer

이승우,정용철,한현대,박정기 한국고분자학회 2006 한국고분자학회 학술대회 연구논문 초록집 Vol.2006 No.4

토양 수분 조건에 따른 블랙쵸크베리 ‘Nero’ 과실의 품질 및 세포 발달 비교

원정연,신현석,오영재,한현대,김금선,오세원,김대일 한국자원식물학회 2017 한국자원식물학회지 Vol.30 No.1

Effects of different soil water conditions on fruit characteristics were investigated in 5-year-old ‘Nero’ black chokeberry trees (Aronia melanocarpa). Three kinds of drought stresses, including low water deficit, severe water deficit, and very severe water deficit, due to decline of soil water decreased the fruit quality of weight of 10 berries, soluble solid content, and anthocyanin, compared with the control (consistent water supply) during the harvest period. After longer drought stress, supply of soil water could induce berry cracking because cell size of epidermis of fruits contracted, whereas cell size of sub-epidermis and flesh expanded. Thus periodic water supply using water supply facility is needed for yield and quality of black chokeberry ‘Nero’ fruits Key words – Aronia melanocarpa, Black chokeberry, Cell enlargement, Drought 토양수분의 변화가 블랙쵸크베리 ‘Nero’의 과실 특성에 미치는 영향을 조사하여 고품질 블랙쵸크베리 재배 연구의 기초자료를 제공하고자 수행하였다. 토양수분 감소로 인한 건조스트레스는 수확기 동안 블랙쵸크베리의 과실의 과중, 당도, 안토시아닌 함량 등의 품질을 저하시켰다. 또한 건조기간이 길수록 이후 토양수분 공급시, 과실 표피 세포 크기는 감소하는 반면 아표피, 과육 세포의 크기가 증가하여 열과를 유발할 수 있다. 따라서 관수시설을 통한 일정 주기의 관수가 블랙쵸크베리 과실 생산량 및 품질 향상을 위해 필요할 것으로 생각된다.

질소시비농도에 따른 1년생 사과 ‘Fuji’/M.9 포트묘목의 수체 생장

하웅용,신현석,임헌규,오영재,한현대,김금선,오세원,권의석,김대일 한국자원식물학회 2019 한국자원식물학회지 Vol.32 No.5

The study was carried out to investigate growth of 48.6-L pot-cultivated 1-year-old ‘Fuji’/M.9 apple treesdepending on different levels of nitrogen concentration. While rise in tree growth was paralleled with increase of nitrogenconcentration, more than 32 mM of nitrogen rather restrained tree growth. In particular, growth of 16 mM of nitrogen treatedtrees was satisfied with criteria for production of high-quality pot-cultivated nursery stocks. Although mineral contents ofleaves were higher in 8 and 16 mM nitrogen treatments than commonly recommended mineral contents in apple orchards,such somewhat surplus minerals could be helpful for tree growth after transplanting to apple orchards. In addition, our resultindicated that soils of 8 and 16 mM of nitrogen treated pots met appropriate criteria for soil chemical property of appleorchards. Thus, in the light of tree growth, mineral contents of leaves, and soil chemical property in the pots, 16 mM ofnitrogen treatment is considered to be suitable for production of 1-year-old ‘Fuji’/M.9 apple potted trees. 사과 ‘후지’/M.9 포트묘목 개발을 위해 질소시비농도에 따른생장특성을 조사하고, 포트 우량묘목 생산에 적합한 질소시비농도와 잎의 무기영양성분 함량 및 토양 화학성의 안정성을 확인하였다. 질소시비농도가 높아질수록 묘목의 생장은 증가되었고, 특히16 mM 처리가 수체 생장에 가장 좋았으며, 우량묘목판단기준에 부합하였다. 32 mM 이상의 고농도는 오히려 생장을 감소시켰다. 잎의 무기영양성분 함량은 8, 16 mM 처리구에서 기존 사과과원의 적정수준보다 높았고, 이러한 무기영양성 분은 정식 후 수체 생장에 도움이 될 것으로 생각되었다. 토양화학성 또한 8, 16 mM 처리구에서 안정적이었다. 따라서 수체생육, 잎의 무기영양성분, 포트 내 토양화학성을 고려한 결과,사과 ‘후지’/M.9 우량 포트묘목 생산을 위한 적정 질소시비량은16 mM로 판단되었다.

재배지역 및 고도에 따른 블랙초크베리 ‘Nero’의 수체 생육 및 과실 특성

원정연,신현석,오영재,한현대,권의석,김대일 한국자원식물학회 2018 한국자원식물학회지 Vol.31 No.2

The study was performed to investigate basic tree growth and fruit characteristics of ‘Nero’ black chokeberry (Aronia melanocarpa) depending on the different cultivation regions and altitudes in 2014, 2015. Tree growth and change of developmental stages of ‘Nero’ were similar regardless of meteorological environment of the four cultivation regions (Danyang, Okcheon, Yeongdong, and Geumsan) in 2014. Fruit characteristics of ‘Nero’ were significant differences in berry weight and anthocyanin content among the four orchards due to difference in cultivation techniques and relatively wide daily temperature range just before harvest, respectively. Tree growth and change of developmental stages of ‘Nero’ grown at the two orchards with different altitudes appeared to be similar during the successive years 2014 and 2015. Soluble solids content of the berries cultivated at low altitude (117 m) was higher than at high altitude (342 m). Acidity showed an inverse pattern with soluble solids content. Anthocyanin content increased progressively until at the middle of August, 2014-15 and then it decreased. Our results showed that black chokeberry is a species adaptive to the domestic environment as there were no differences in tree growth and change of developmental stages of ‘Nero’. Considering fruit quality of black chokeberry such as soluble solids content, acidity, and anthocyanin content, our results suggest that optimal harvest period of black chokeberry ‘Nero’ is August 8 to 19. 재배지와 고도에 따른 블랙초크베리 ‘Nero’의 생육 시기, 수체 생육 및 과실 특성을 구명하여 육종 및 재배·생리 연구에기초자료를 제공하고자 수행하였다. 2014년 재배지별 블랙초크베리 생육 시기 및 수체 생육은 네 재배 지역(단양, 옥천, 영동, 금산)의 기상 환경에 관계없이 유사했다. 재배지별 과실특성은 십립중과 안토시아닌 함량에서 유의적인 차이가 나타났는데 이는 각각 전정방법과 관수 등의 재배 기술과 수확 전 일교차 급증의 영향으로 추측되었다. 2014년과 2015년 고도별 블랙초크베리 ‘Nero’의 생육 시기 및 수체 생육은 유사하게 나타났지만, 과실 특성은 기온이 높은 저지대(117 m)에서 생산된 과실의 가용성고형물이 고지대(342 m)보다 높았던 반면, 산도는 고지대가 높았다. 안토시아닌 함량은 2014년과 2015년 8월 중순까지 증가하다가 고도별로 차이는 있으나 이후부터 감소하는 경향을 보였다. 재배지와 고도별 블랙초크베리 ‘Nero’의 수체 생육및 생육 시기는 차이가 없어 국내 환경에서 재배가 용이한 것으로 생각되며, 수확 시기별 과실 특성을 고려할 때 단양 지역의 블랙초크베리의 최적 수확 시기는 8월 8-19일로 판단되었다.

배나무(Pyrus spp.) 유전체 연구 현황

오영재,신현석,김금선,한현대,김윤경,김대일 한국식물생명공학회 2015 식물생명공학회지 Vol.42 No.4

Based on the place of its origin, pear tree (Pyrus spp.) is largely divided into European pears (P. communis, cultivated mainly in Europe and the U.S.) and Asian pears (P. pyrifolia, P. bretschneideri, and P. ussuriensis, distributed and grown in East Asian countries including China, Japan, and Korea). Most pear trees have 17 chromosomes (diploidy, 2n=2x=34). Their genetic studies and precise cultivar breeding are highly restricted by conditions such as self-incompatibility controlled by S-locus and juvenility as one major character of fruit crops. Genetic studies on Pyrus have been promoted by the development of various molecular markers. These markers are being utilized actively in various genetic studies, including genetic relationship analysis, genetic mapping, and QTL analysis. In addition, research on pear genetic linkage maps has been extended to studies for the identification of QTL for target traits such as disease resistance and genetic loci of useful traits. NGS technology has radically reduced sequencing expenses based on massive parallel reactions to enable high-capacity and high-efficiency. NGS based genome analyses have been completed for Chinese pear ‘Danshansuli’ and European pear ‘Bartlett’. In Korea, GWAS for agricultural valuable traits such as floral structure, ripening, and total soluble contents have been conducted through resequencing. GBS has been performed for ‘Whangkeumbae’, ‘Cheongsilri’, and ‘Minibae’.

단양 재배지에 따른 ‘Nero’ 아로니아의 수체 생육 및 과실 특성 비교

원정연(Jungyeon Won),오영재(Youngjae Oh),김금선(Keumsun Kim),오세원(Sewon Oh),한현대(Hyeondae Han),권의석(Yeuseok Kwon),박난수(Nansu Park),김대일(Daeil Kim) 한국원예학회 2015 한국원예학회 학술발표요지 Vol.2015 No.10

토양 수분 변화에 따른 아로니아의 수체 생육 및 과실 특성

원정연(Jungyeon Won),오영재(Youngjae Oh),김금선(Keumsun Kim),오세원(Sewon Oh),한현대 (Hyeondae Han),안수지(Suji Ahn),하웅용(Woongyong Ha),김대일(Daeil Kim) 한국원예학회 2016 한국원예학회 학술발표요지 Vol.2016 No.5