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우진석,Chung-Hyun Cho,Do Han Kim,이은희 생화학분자생물학회 2010 Experimental and molecular medicine Vol.42 No.9
During membrane depolarization associated with skeletal excitation-contraction (EC) coupling, dihydropyridine receptor [DHPR, a L-type Ca2+ channel in the transverse (t)-tubule membrane] undergoes conformational changes that are transmitted to ryanodine receptor 1 [RyR1, an internal Ca2+-release channel in the sarcoplasmic reticulum (SR) membrane] causing Ca2+ release from the SR. Canonical-type transient receptor potential cation channel 3 (TRPC3), an extracellular Ca2+-entry channel in the t-tubule and plasma membrane, is required for full-gain of skeletal EC coupling. To examine additional role(s) for TRPC3 in skeletal muscle other than mediation of EC coupling,in the present study, we created a stable myoblast line with reduced TRPC3 expression and without α1SDHPR (MDG/TRPC3 KD myoblast) by knock-down of TRPC3in α1SDHPR-null muscular dysgenic (MDG) myoblasts using retrovirus-delivered small interference RNAs in order to eliminate any DHPR-associated EC coupling-related events. Unlike wild-type or α1SDHPR-null MDG myoblasts, MDG/TRPC3 KD myoblasts exhibited dramatic changes in cellular morphology (e.g., unusual expansion of both cell volume and the plasma membrane, and multi-nuclei) and failed to differentiate into myotubes possibly due to increased Ca2+ content in the SR. These results suggest that TRPC3 plays an important role in the maintenance of skeletal muscle myoblasts and myotubes.
Mitochondrial Transplantation Ameliorates the Development and Progression of Osteoarthritis
이아람,우진석,이선영,나현식,조권형,이연수,이정수,김선애,박승환,김석중,조미라 대한면역학회 2022 Immune Network Vol.22 No.2
Osteoarthritis (OA) is a common degenerative joint disease characterized by breakdown of joint cartilage. Mitochondrial dysfunction of the chondrocyte is a risk factor for OA progression. We examined the therapeutic potential of mitochondrial transplantation for OA. Mitochondria were injected into the knee joint of monosodium iodoacetate-induced OA rats. Chondrocytes from OA rats or patients with OA were cultured to examine mitochondrial function in cellular pathophysiology. Pain, cartilage destruction, and bone loss were improved in mitochondrial transplanted-OA rats. The transcript levels of IL-1β, TNF-α, matrix metallopeptidase 13, and MCP-1 in cartilage were markedly decreased by mitochondrial transplantation. Mitochondrial function, as indicated by membrane potential and oxygen consumption rate, in chondrocytes from OA rats was improved by mitochondrial transplantation. Likewise, the mitochondrial function of chondrocytes from OA patients was improved by coculture with mitochondria. Furthermore, inflammatory cell death was significantly decreased by coculture with mitochondria. Mitochondrial transplantation ameliorated OA progression, which is caused by mitochondrial dysfunction. These results suggest the therapeutic potential of mitochondrial transplantation for OA.
A focus on extracellular Ca2+ entry into skeletal muscle
조정현,우진석,Claudio F Perez,이은희 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-
The main task of skeletal muscle is contraction and relaxation for body movement and posture maintenance. During contraction and relaxation, Ca2+ in the cytosol has a critical role in activating and deactivating a series of contractile proteins. In skeletal muscle, the cytosolic Ca2+ level is mainly determined by Ca2+ movements between the cytosol and the sarcoplasmic reticulum. The importance of Ca2+ entry from extracellular spaces to the cytosol has gained significant attention over the past decade. Store-operated Ca2+ entry with a low amplitude and relatively slow kinetics is a main extracellular Ca2+ entryway into skeletal muscle. Herein, recent studies on extracellular Ca2+ entry into skeletal muscle are reviewed along with descriptions of the proteins that are related to extracellular Ca2+ entry and their influences on skeletal muscle function and disease.
Streptomyces sp. GCA0001로 부터의 신규 항생물질 Cystocin의 구조분석, 생물활성 및 유도체제조
김자용,이희찬,우진석,송재경 호서대학교 반도체제조장비국산화연구센터 2001 반도체장비학술심포지움 Vol.2001 No.-
본 발명의 Puromycin 유도체인 Cystocin 화합물은 유기 합성에 의해 제조된 물질이 아니라 방선균계열의 신균주인 Streptomyces sp GCA0001로부터 추출된 신규 물질로서, 항박테리아, 항종양 및 항바이러스 활성 등의 생물학적 미생물 활성면에서 종래의 Puromycin 화합물에 비해 현저히 뛰어난 효과를 지니고 있고 Streptomyces sp GCA0001로부터 추출, 분리 및 정제 과정을 통해 제조된 자연의 선택의 과정을 거친 화합물이므로, Puromycin을 대체할 수 있는 획기적인 물질로 볼 수 있다. Cystocin, a derivative of Puromycin, is a new material derived from Streptomyces sp GCA0001, new strain of Actinomycetes spiecies.This compound has outstanding biological activities in anti-bacteria, anti-tumor and anti-virus than former Puromycin compounds.And it is chosen by natural selection processing through extraction, isolation and purification from, so it may replace old Puromycins in most applications.
Jhun JooYeon,우진석,Kwon Ji Ye,Na Hyun Sik,Cho Keun-Hyung,김선애,Kim Seok Jung,문수진,Park Sung-Hwan,조미라 대한면역학회 2022 Immune Network Vol.22 No.4
Osteoarthritis (OA) is the most common form of arthritis associated with ageing. Vitamin D has diverse biological effect on bone and cartilage, and observational studies have suggested it potential benefit in OA progression and inflammation process. However, the effect of vitamin D on OA is still contradictory. Here, we investigated the therapeutic potential of vitamin D in OA. Six-week-old male Wistar rats were injected with monosodium iodoacetate (MIA) to induce OA. Pain severity, cartilage destruction, and inflammation were measured in MIA-induced OA rats. Autophagy activity and mitochondrial function were also measured. Vitamin-D (1,25(OH)2D3) and celecoxib were used to treat MIA-induced OA rats and OA chondrocytes. Oral supplementation of vitamin D resulted in significant attenuations in OA pain, inflammation, and cartilage destruction. Interestingly, the expressions of MMP-13, IL-1β, and MCP-1 in synovial tissues were remarkably attenuated by vitamin D treatment, suggesting its potential to attenuate synovitis in OA. Vitamin D treatment in OA chondrocytes resulted in autophagy induction in human OA chondrocytes and increased expression of TFEB, but not LC3B, caspase-1 and -3, in inflamed synovium. Vitamin D and celecoxib showed a synergistic effect on antinociceptive and chondroprotective properties in vivo. Vitamin D showed the chondroprotective and antinociceptive property in OA rats. Autophagy induction by vitamin D treatment may be a promising treatment strategy in OA patients especially presenting vitamin D deficiency. Autophagy promoting strategy may attenuate OA progression through protecting cells from damage and inflammatory cell death.
Streptomyces sp GCA0001로부터 Cystocin의 생산 및 분리 정제
김동현,이희찬,우진석,송재경,류광경,김대희,강선엽 호서대학교 반도체제조장비국산화연구센터 2001 반도체장비학술심포지움 Vol.2001 No.-
Cystocin을 생산하는 Streptomyces sp GCA0001균주를 screenig하였으며, Streptomyces sp GCAO001를 배양하고 분리정제를 통하여 흰색 분말의 Cystocin을 얻을 수 있었다.Cystocin은 Streptomyces alboniger에서 생산되는 Puromycin과 매우 유사한 구조의 항생물질로 확인되었다.Cystocin은 Puromycin과 마찬가지로 단백질 합성시 폴리펩티드 사슬의 elongation을 조기 종결함으로써 생체내에서 항박테리아, 항종양 및 항바이러스 활성을 나타낸다. Cystocin is identified from the screening of Streptomyces sp GCAO001.It is isolated from the culture of Streptomyces sp GCAO001.A white coloured cystocin is structurally similar to puroinycin, an antibiotic produced by streptomyces alboniger.Like puromycin, cystocin possesses an antibacterial, antitumour and antiviral activity.It impairs the protein synthesis by inhibiting polypeptide chain elongation.
임재걸 ( Jaegeol Yim ),우진석 ( Jin-seok Woo ) 한국정보처리학회 2012 한국정보처리학회 학술대회논문집 Vol.19 No.1
본 논문에서는 스마트폰에 장착된 여러 가지 센서들의 센서 값을 수집하는 안드로이드 애플리케이션을 소개하고, 실제로 수집한 데이터를 분석한다. 분석을 위하여 일반적인 통계 분석 도구인 R을 사용한다. 분석 결과 어떤 센서 (Azimuth를 비롯한 orientation 센서) 값은 실용성이 있는 수준으로 정확하다는 것을 알 수 있었으나 또 다른 센서는 (밝기 센서 등) 잡음이 매우 심함을 보인다.