H9c2 심근세포에서 제주모시풀(Boehmeria quelpaertense)로부터 분리된 flavonoids의 H2O2로 유도된 독성 보호 효과

우경완,심미옥,박호,정호경,안병관,함성호,박종혁,조현우 한국자원식물학회 2018 한국자원식물학회지 Vol.31 No.1

제주모시풀에서 각종 column chromatography법을 이용하여 2종의 flavonoid를 분리하여, 1H-, 13C-NMR, LC ESI-ITTOF MS를 통해 구조동정 할 수 있었다. 분리된 물질이 심장세포에서 높은 항산화 활성을 가지고 있으며, 활성 산소종의 작용기전 연구 및 심근경색 질환의 예방과 치료에 효과적으로 사용할 수 있는 기초자료가 될 것으로 사료된다. As a part of an infrastructure project on medicinal herb-based remedies, we conducted a phytohemical investigation of the 100% MeOH extract from the aerial part of Boehmeria quelpaertense; our findings resulted in the isolation of flavonoids (1-2), isoquercitrin (1) and hyperoside (2). The identification and structural elucidation of these compounds were based on 1 H-, 13 C-NMR, and LC ESI IT-TOF MS data. All the compounds isolated from this plant were reported for the first time. In this study, we examined the antioxidant activity of the 1 and 2 on the hydrogen peroxide (H2O2)-induced oxidative stress in a Rat Cardiomyoblast cell line (H9c2). The pretreatment of the flavonoids showed that it protects against H2O2-mediated cell death in the H9c2 cell line. Also, it decreases the intracellular reactive oxygen species (ROS) levels by the flavonoids in the H2O2-treated H9c2 cell line. These results showed that the 1 and 2 are a source of antioxidants. As a result, they might be helpful in preventing the progress of various oxidative stress mediated diseases, including myocardial infarction.

Phytochemical Constituents from the Rhizomes of Osmunda japonica Thunb and Their Anti-oxidant Activity

우경완,정자균,이현주,김태묵,김민석,정호경,안병관,함성호,전병훈,조현우 한국생약학회 2017 Natural Product Sciences Vol.23 No.3

Eleven compounds (1-11) were isolated from the rhizomes of Osmunda japonica, and their structures were elucidated based on 1H, 13C-NMR and LC-IT-TOF MS data. Of these compounds, all compounds (1 - 11) have been previously reported, although five (6 - 9, 11) have not previously been isolated from this plant. The antioxidant activities of isolated compounds (1 - 11) were measured by DPPH and ABTS assays, and compound 10 showed the high antioxidant activity.

Phytochemical Constituents of Allium victorialis var. platyphyllum

우경완,이강노 한국생약학회 2013 Natural Product Sciences Vol.19 No.3

Phytochemical investigation of the 80% MeOH extract from the leaves of Allium victorialis var. platyphyllum resulted in the isolation of seventeen compounds; two terpenes, three norsesquiterpenes, one furofuran lignan, and eleven phenolic derivatives. Their chemical structures were characterized by spectroscopic methods to be trans-phytol (1), phytene-1,2-diol (2), icariside B2 (3), (6S,9S)-roseoside (4), sedumoside G (5), pinoresinol-4-O-glucoside (6), 2-methoxy-2-(4'-hydroxyphenyl)ethanol (7), 2-hydroxy-2-(4'-hydroxyphenyl)ethanol (8), Benzyl b-D-glucopyranoside (9), methyl ferulate (10), trans-ferulic acid (11), methyl-p-hydroxycinnamate (12), glucosyl methyl ferulate (13), linocaffein (14), siringin (15), 2-(4-hydroxy-3-methoxyphenyl)-ethyl-O-b-D-glucopyranoside (16), and pseudolaroside C (17). All compounds were isolated for the first time from this plant.

떡윤노리나무로부터 분리된 Lyoniside의 함량분석과 항염증 효과

우경완,안병관,성태경,이혜미,장지훈,이기호,조현우,조정희 한국생약학회 2016 생약학회지 Vol.47 No.1

In this study, the purification of the MeOH extract from the stems and leaves of Pourthiaea villosa var. brunnea using column chromatography furnished a main compound, lyoniside. The structure was elucidated on the basis of 1H and 13C NMR spectroscopic data. Quantitative analysis of lyoniside was conducted by HPLC method and the highest content of lyoniside was found in 50% MeOH reflux extraction. To investigate the anti-inflammatory effect of the lyoniside, we measured nitric oxide and pro-inflammatory cytokines such as TNF-a, IL-1b, IL-6 levels in lipopolysaccharide-induced murine macrophage cell line RAW 264.7. As a results, lyoniside decreased the level of nitric oxide and IL-6 in concentration dose dependent manner in RAW 264.7 cells.

배롱나무의 항산화 활성 성분

우경완,이강노,심미옥,박종일,김민석,서원세,조현우,권학철,박종철 한국생약학회 2016 생약학회지 Vol.47 No.3

Phytochemical investigation of the 80% MeOH extract from the stems of Lagerstroemia indica resulted in the isolation of eighteen compounds; four norsesquiterpenes, fourteen phenolic derivatives. Their chemical structures were characterized by spectroscopic methods to be tachioside (1), isotachioside (2), 2,4,6-trimethoxyphenyl b-D-glucopyranoside (3), gallic acid 4-methyl ether (4), protocatechuic acid (5), gallic acid (6), vanillic acid (7), vanillin (8), 2-methoxy-5-hydroxymethyl-phenyl-1-O-(6''-galloyl)-b-D-glucopyranoside (9), 2,4,6-trimethoxyphenol-1-O-b-D-(6'-O-galloyl)-glucopyranoside (10), 4-hydroxy-3-methoxyphenyl-1-O-(6'-O-galloyl)-b-D-glucopyranoside (11), vomifoliol (12), vomifoliol 9-O-b-D-glucopyranoside (13), 6R,​9R-​3-​oxo-​α-​ionol-​9-​O-​b-​D-​glucopyranoside (14), dihydrophaseic acid 4'-O-b-D-glucopyranoside (15), b-hydroxypropiovanillone 3-O-b-D-glucopyranoside (16), myrciaphenone A (17), and coumaric acid (18). Compounds 1-5 and 7-18 were isolated for the first time from this plant. Compounds 1-18 were investigated for their antioxidant properties using DPPH and ABTS radical scavenging capacity assay, Fe2+ chelating, and FRAP assay. It was found that 4, 6, and 11 possessed the highest antioxidant capacities.

자란초에서 분리된 Luteolin 5-glucoside의 함량분석과 신경세포 보호 활성

우경완,조현우,심미옥,김아현,강병만,정호경,안병관,조정희 한국생약학회 2016 생약학회지 Vol.47 No.3

In the course of our continuing search for biologically active components from Korean medicinal plants, we isolated the main compound, luteolin 5-glucoside from aqueous fraction of Ajuga spectabilis. The structure was elucidated by the basis of 1H and 13C NMR and TOF ESI-MS data. Quantitative analysis of luteolin 5-glucoside was carried out on a XBridge C18 column (S-5 mm, 4.6×250 mm) with gradient elution composed of acetonitrile:water. The results exhibit that the average content of main compound in A. spectabilis were 0.048%. Oxidative stress plays a major role Alzheimer’s disease (AD) and other neurodogenerative disease. AD is major health problem and there is currently no clinically accepted treatment to cure or stop its progression. Pretreatment with luteolin 5-glucoside markedly attenuated H2O2-induced cell viability loss in a dose-dependent manner. Luteolin 5-glucoside also inhibited the formation of intracellular reactive oxygen species in SH-SY5Y. The results suggest that luteolin 5-glucoside from A. spectabilis has protective effects against oxidative stress-induced cytotoxicity, which might be a potential therapeutic compound for treating and/or preventing neurodegenerative disease implicated with oxidative stress.

A New Lignan Glycoside from Juniperus rigida

우경완,최상운,박종철,이강노 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.12

A new lignan glycoside, named juniperigiside (1) was isolated from the CHCl3 soluble fraction of the MeOH extract of stems and leaves of Juniperus rigida S.et Z. Compound 1 was identified by 1D- and 2D-NMR spectroscopy as well as CD analysis as (2R,3S)-2,3-dihydro-7-methoxy-2-(4'-hydroxy-3'-methoxyphenyl)-3-hydroxymethyl-5-benzofuranpropanol 4'-O-(3-O-methyl)-α-L-rhamnopyranoside. Five known lignans, icariside E4 (2), desoxypodophyllotoxin (3), savinin (4), thujastandin (5), and (−)-nortrachelogenin (6) in addition to five known labdane diterpenes including trans-communic acid (7), 13-epi-torulosal (8), 13-epi-cupressic acid (9), imbricatoric acid (10), and isocupressic acid (11) were also isolated and their structures were characterized by comparing their spectroscopic data with those in the literature. All compounds were isolated for the first time from this plant, and 5 and 6 were first reported from the genus Juniperus. The isolated compounds were tested for cytotoxicity against four human tumor cell lines in vitro using a Sulforhodamin B bioassay. Compounds 3, 4, 7, and 8 showed considerable cytotoxicity against four human cancer cell lines in vitro.

Two New ent-Kaurane Diterpenoids from the Roots of Fritillaria thunbergii

박종열,이성영,우경완,이제현,이강노 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.5

결명자로부터 분리된 Aurantio-obtusin의 항염증 활성

이기호,장지훈,우경완,노종현,정호경,용주현,안병관 한국생약학회 2019 생약학회지 Vol.50 No.1

Cassia tora L. have been used as a folk medicine in Korea. This study investigated anti-inflammatory effect of aurantio-obtusin isolated from C. tora. We isolated aurantio-obtusin from 50% ethanol extracts of C. tora L. We investigated the anti-inflammatory effects of aurantio-obtusin on the lipopolysaccharide (LPS)-stimulated inflammatory response in murine macrophage cell line (Raw 264.7). To investigate the cytotoxicity of aurantio-obtusin on RAW 264.7 cells, MTS assay was performed. RAW 264.7 cells were treated with aurantio-obtusin at different concentrations (12.5, 25, 50, 100 μM) for 30 h. The result showed that aurantio-obtusin had no cytotoxic effect in a concentration range of 12.5 – 100 μM. To determine the effect of aurantio-obtusin on LPS-induced NO production, the NO concentration measurement was performed. RAW 264.7 cells were treated with aurantio-obtusin at 12.5, 25, 50 and 100 μM for 24 h, and the results showed that the NO production of aurantio-obtusin-treated cells compared to LPS alone treated group was significantly decreased in a dose-dependent manner. Pretreatment of aurantio-obtusin inhibited LPS-induced NO production in a dose-dependent manner. To find out inhibitory mechanisms of aurantio-obtusin on inflammatory mediators, we examined the PGE2 pathways. As a result, PGE2 were decreased in a dose-dependent manner by aurantio-obtusin. The release of interleukin-1b (IL-1b) and IL-6 were also reduced. Moreover, aurantio-obtusin suppressed LPL-induced IκB-a degradation. These results suggest that the down regulation of NO, PGE2, IL-1b and IL-6 expression by aurantio-obtusin are achieved by the downregulation of NF-κB activity.

노루오줌으로부터 Bergenin의 함량 분석 및 에탄올 추출물의자궁경부암세포 성장 억제 효과

김아현,노종현,우경완,강주은,심미옥,김민석,함성호,조현우 한국생약학회 2018 생약학회지 Vol.49 No.1

Astilbe rubra (AR) is a perennial, belongs to the Saxifragaceae family, it contains tannin and triterpene. AR has been used in republic korea to improve toxication, fever, pain and convulsion. Recently, number of natural products have been analyzed for potential pharmacological activities including anti-cancer, anti-obesity and anti-diabetic medication. Consequently, we investigated the growth inhibition effect of Astilbe rubra water extract (WAC), ethanol extract (EAC) and bergenin on Hela cell (human adenocarcinoma cell). From whole plant of A. rubra, bergenin was isolated by column chromatography and its structures were identified by 1H, 13C NMR and IT TOF-ESI MS. High extraction efficiency of bergenin was shown at 0.95% under 60 min reflux extraction with 50% MeOH. The MTS assay showed that EAC (ethanol extract) treatment increased cell death in a dose-dependent manner. Moreover, EAC treatment on Hela cell increased apoptotic cell death and caspase-3 activity. Results suggest that EAC has growth inhibition effect on Hela cells, but not WAC and bergenin. 500 μg/mL EAC treatment inhibited Hela cell at 60.2±1.5%.