Polydioxanone/pluronic F127 담체에 유입된 골막기원세포의 조골활성

이진호,오세행,박봉욱,하영술,김덕룡,김욱규,김종렬,변준호,Lee, Jin-Ho,Oh, Se-Heang,Park, Bong-Wook,Hah, Young-Sool,Kim, Deok-Ryong,Kim, Uk-Kyu,Kim, Jong-Ryoul,Byun, June-Ho 대한악안면성형재건외과학회 2009 Maxillofacial Plastic Reconstructive Surgery Vol.31 No.6

Three-dimensional porous scaffolds play an important role in tissue engineering strategies. They provide a void volume in which vascularization, new tissue formation, and remodeling can occur. Like any grafted materials, the ideal scaffold for bone tissue engineering should be biocompatible without causing an inflammatory response. It should also possess biodegradability, which provides a suitable three-dimensional environment for the cell function together with the capacity for gradual resorption and replacement by host bone tissue. Various scaffolds have already been developed for bone tissue engineering applications, including naturally derived materials, bioceramics, and synthetic polymers. The advantages of biodegradable synthetic polymers include the ability to tailor specific functions. The purpose of this study was to examine the osteogenic activity of periosteal-derived cells in a polydioxanone/pluronic F127 scaffold. Periosteal-derived cells were successfully differentiated into osteoblasts in the polydioxanone/pluronic F127 scaffold. ALP activity showed its peak level at 2 weeks of culture, followed by decreased activity during the culture period. Similar to biochemical data, the level of ALP mRNA in the periosteal-derived cells was also largely elevated at 2 weeks of culture. The level of osteocalcin mRNA was gradually increased during entire culture period. Calcium content was detactable at 1 week and increased in a time-dependent manner up to the entire duration of culture. Our results suggest that polydioxanone/pluronic F127 could be a suitable scaffold of periosteal-derived cells for bone tissue engineering.

인간 골막기원세포와 Polydioxanone/Pluronic F127 담체를 이용한 골형성

박봉욱,이진호,오세행,김상준,하영술,전령훈,맹건호,노규진,김종렬,변준호,Park, Bong-Wook,Lee, Jin-Ho,Oh, Se-Heang,Kim, Sang-June,Hah, Young-Sool,Jeon, Ryoung-Hoon,Maeng, Geun-Ho,Rho, Gyu-Jin,Kim, Jong-Ryoul,Byun, June-Ho 대한악안면성형재건외과학회 2012 Maxillofacial Plastic Reconstructive Surgery Vol.34 No.6

Purpose: The purpose of this study is to examine in vivo osteogenesis of cultured human periosteal-derived cells and polydioxanone/pluronic F127 scaffold. Methods: Two one-year-old miniature pigs were used in this study. $2{\times}10^6$ periosteal-derived cells in 1 mL medium were seeded by dropping the cell suspension into the polydioxanone/pluronic F127 scaffold. These cell-scaffold constructs were cultured in osteogenic Dulbecco's modified Eagle's medium for 7 days. Under general anesthesia with azaperone and tiletamine-zolazepam, the mandibular body and ramus of the pigs were exposed. Three bony defects were created. Polydioxanone/pluronic F127 scaffold with periosteal-derived cells and the scaffold only were implanted into each defect. Another defect was left empty. Twelve weeks after implantation, the animals were sacrificed. Results: New bone formation was clearly observed in the polydioxanone/pluronic F127 scaffold with periosteal-derived cells. Newly generated bone was also observed in the scaffold without periosteal-derived osteoblasts and empty defect, but was mostly limited to the periphery. Conclusion: These results suggest that cultured human periosteal-derived cells have good osteogenic capacity in a polydioxanone/pluronic F127 scaffold, which provides a proper environment for the osteoblastic differentiation of these cells.

탈세포 장기 지지체의 제조 및 분석기법

전소영 ( So Young Chun ),오세행 ( Se Heang Oh ),유지 ( James J Yoo ),권태균 ( Tae Gyun Kwon ) 한국조직공학과 재생의학회 2015 조직공학과 재생의학 Vol.12 No.1s

Organ transplantation has often been successful for treatment of end-stage organ failure. However, the shortage of donor organ still remains problematic in clinical practices. As an alternative, the tissue-engineering approach for functional organ replacement has been extensively studied. More recently, decellularized organs have been emerged as a promising scaffold for reconstruction of the complicated organs (e.g., heart, liver, lung and kidney). The ideal decellularized organ scaffolds need to contain extracellular matrix (ECM), bioactive molecules, vascular systems and tissue microarchitecture. To fulfill these requirements, physical, chemical, and biological techniques have been adapted in the process of organ decellularization. In this review, the representative techniques for the organ decellularization and their characterization as well as considerations for implantation are discussed.

기상 UV 그라프트 반응에 의한 고분자 필름의 표면 개질

오승희 ( Seung Hee Oh ),오세행 ( Se Heang Oh ),이진호 ( Jin Ho Lee ) 한국 접착 및 계면학회 2000 접착 및 계면 Vol.1 No.1

원심분리법을 이용한 다공 크기 구배를 가지는 알지네이트 지지체의 제조 및 분석

박일규 ( Il Kyu Park ),오세행 ( Se Heang Oh ),이진호 ( Jin Ho Lee ) 한국조직공학과 재생의학회 2004 조직공학과 재생의학 Vol.1 No.2

It is well recognized that the pore size of scaffolds plays an important role for tissue ingrowth and regeneration: different kinds of cells were shown to have different optimal pore size ranges in the scaffolds for effective cell growth. So, if the tissue scaffold with pore size gradient (i. e., the scaffold with gradually increasing pore sizes along one direction) can be prepared, it will be of particular interest for basic studies of the interaction between tissue cells and scaffolds since the effect of pore size can be examined in a single experiment using one scaffold (pore size gradient scaffold). In recent years, several techniques have been used to fabricate porous polymer scaffolds having 3-dimensional pore structure. However, it is not possible to fabricate scaffolds with pore size gradient from those techniques. In this study, we developed a new method to fabricate pore size gradient scaffolds by a simple centrifugation. We fabricated alginate cylindrical scaffolds with gradually increasing pore size (80~310 μm) along the longitudinal direction by the centrifugation method. In this method, the pore size ranges of the scaffold could be easily controlled by adjusting centrifugal force. The prepared alginate scaffolds were impregnated into 1 wt% chitosan solution to improve cell adhesiveness as well as mechanical strengths. This study demonstrate that the centrifugation method is a simple and effective method to prepare tissue scaffolds with controllable pore size ranges.

Porous Matrices with Leaf-Stacked Structure for Tissue Engineering Applications

김호용,김민지,박진현,안보슬,이재훈,이진호,변준호,오세행(Se Heang Oh) 한국고분자학회 2019 한국고분자학회 학술대회 연구논문 초록집 Vol.44 No.1

반월상 연골 재생을 위한 친수화 처리 폴리다이옥사논 지지체의 제조 및 분석

조광준 ( Kwang Joon Cho ),송대근 ( Dae Kun Song ),오세행 ( Se Heang Oh ),고영주 ( Young Joo Koh ),이상훈 ( Sahng Hoon Lee ),이명철 ( Myung Chul Lee ),이진호 ( Jin Ho Lee ) 한국조직공학과 재생의학회 2005 조직공학과 재생의학 Vol.2 No.2

Biodegradable polymers have been widely used to fabricate porous scaffolds for three-dimensional (3-D) cell cultures to regenerate tissue-based artificial organs. Until now, poly(L-lactic acid), poly(glycolic acid), and poly(lactic-co-glycolic acid) as synthetic biodegradable polymers have been most widely used to fabricate 3-D scaffolds. However, these polymers have limitations for some applications because of their brittleness, hydrophobicity, and low cell adhesiveness. Recently polydioxanone (PDO) has been interested in the applications for tissue regeneration since this polymer has good elasticity as well as biocompatibility and biodegradability. However, porous scaffold fabrications from PDO were limited due to its poor solubility (solvents to dissolve PDO are few). In this study, we firstly fabricated porous PDO scaffolds (disc and meniscus shapes) using a melt-molding particulate-leaching method developed by our laboratory. This method does not need any solvents during the fabrication process. To improve the hydrophilicity and cell compatibility of the scaffolds, PDO was blended with polyvinyl alcohol (PVA) with different ratio. The hydrophilicity, morphology, porosity, and mechanical properties of the prepared PDO scaffolds were investigated. The PDO/PVA (5 wt%) blend scaffold was found to have sufficient hydrophilicity, mechanical strength, and elasticity for tissue engineering applications including meniscus regeneration.

설포베타인 키토산의 실크 블렌드 필름의 제조 및 그들의 성질

거자성(Ja Sung Koo),차재령(Jae Ryung Cha),오세행(Se Heang Oh),공명선(Moung Seon Gong) 한국고분자학회 2014 폴리머 Vol.38 No.1

Bombyx mori silk fibroin(SF)과 블렌드 필름을 만들기 위하여 키토산에 1,3-propanesultone을 반응시켜 수용성 sulfobetaine chitosan(SCs)을 제조하였다. 여러 가지 비율의 SF/SCs 블렌드 필름을 B. mori SF와 SCs의 수용액을 혼합하여 제조하였다. 수용액으로부터 얻어진 SF/SCs 블렌드 필름의 구조와 형태 변화는 분광학적 및 열적 분석을 통해 규명하였다. SF와 SCs의 혼합 비율에 따른 인공 피부나 화상치료 목적의 비이오재료로서의 물리적 및 기계적 성질에 미치는 영향을 조사하였다. X-선 분석으로 두 생체고분자 사이에 좋은 친화성을 보여주고 있음을 알 수 있었으며 기계적 성질도 SCs의 함량이 증가하면 크게 증가하였다. 37 oC에서 phosphate buffered saline solution용액 중에서 in vitro 분해 실험을 8주 동안 시행한 결과 46.4%가 분해됨을 알 수 있었다. MC3T3-E1 세포에 의한 독성 실험 결과 무독성을 나타내 주었으며, 3일의 배양 후 SF/SCs 필름의 상대 세포 수는 최적화된 tissue cultureplastic보다 약간 낮게 나타남을 알 수 있었다. Water-soluble sulfobetaine chitosan (SCs) was prepared for a blending film with Bombyx mori silk fibroin (SF)by reacting chitosan with 1,3-propanesultone. A series of SF/SCs blended films were successfully prepared by mixingaqueous solutions of B. mori SF and SCs. The SF/SCs blended films were examined through spectroscopic and thermalanalysis to determine the morphological changes of SF in the SCs. The effects of the SF/SCs blend ratios on physicaland mechanical properties were investigated to discover the feasibility of using these films as biomedical materials suchas artificial skin and wound dressing. X-ray analysis showed good compatibility between the two biopolymers. The invitro degradation behavior of the SF/SCs blended films was systematically investigated for up to 8 weeks in phosphatebuffered saline solution at 37 oC and showed a mass loss of 46.4% after 8 weeks. All films showed no cytotoxicity byMC3T3-E1 assay. After 3 days of culture, the relative cell number on all the SF/SCs films was slightly lower than thatof an optimized tissue culture plastic.

성장인자 탑재 주사주입형 생분해성 미립구의 제조 및 이의 조직수복 효능 평가

강준구 ( Jun Goo Kang ),김태호 ( Tae Ho Kim ),오세행 ( Se Heang Oh ),이진호 ( Jin Ho Lee ) 한국조직공학과 재생의학회 2014 조직공학과 재생의학 Vol.11 No.1s

Growth factor [basic fibroblast growth factor (bFGF) or epidermal growth factor (EGF)]-immobilized polycaprolactone (PCL)/Pluronic F127 microspheres were fabricated to investigate their potential use as an injectable bioactive filler for enhancing soft tissue augmentation. It was expected that the microspheres may stimulate the regeneration of soft tissues by the sustained release of the growth factors as well as provide a bulking effect by the injected volume of the microspheres, and thus allow more effective long-term filling effect. The PCL/F127 microspheres were fabricated by an isolated particle-melting method, and the growth factors were easily immobilized onto the surfaces of the PCL/F127 microspheres via heparin binding. From an in vivo animal study, it was observed that the mixture of both bFGF-immobilized and EGF-immobilized microspheres lead to effective soft tissue augmentation.

VEGF/Laminin Co-Immobilized Membrane for Enhanced Differentiation of Muscle-derived Stem Cells into Smooth Muscle Cells

So Ri Lee(이소리),Tae Ho Kim(김태호),Se Heang Oh(오세행),Seong Keun Kwon(권성근),Jin Ho Lee(이진호) 한국고분자학회 2018 폴리머 Vol.42 No.5

본 연구에서는 생리활성인자[vascular endothelial growth factor(VEGF) 및 laminin]가 탑재된, 비대칭구조를 가지는 다공성 polycaporlactone(PCL) 기반의 맴브레인을 제조하였으며, 맴브레인에 탑재된 생리활성인자에 의한 근육유래 줄기세포(muscle-derived stem cells, MDSCs)의 평활근세포(smooth muscle cells, SMCs)로의 분화거동을 연구하였다. PCL 기반 맴브레인에 탑재된 VEGF 및 laminin은 맴브레인으로부터 각각 28일과 14일 동안 지속적으로 방출되었다. 각 생리활성인자가 단독 혹은 공동으로 탑재된 PCL 기반 맴브레인에서 MDSCs의 SMCs로의 분화거동을 DNA 정량화, RT-PCR 및 면역염색을 통해 분석하였으며, 두 생리활성인자가 공동으로 탑재된 PCL 기반 맴브레인에서 가장 우수한 평활근으로의 분화거동을 보임을 확인하였다. 이러한 결과로부터, VEGF/laminin이 탑재된, 비대칭구조를 가지는 다공성 PCL 기반의 맴브레인은 평활근 재생을 위한 재생막으로 사용이 가능할 것이라 판단되었다. It is important to control the stem cell differentiation into target cell types for clinical use of the stem cells. In this study, asymmetrically porous polycaprolactone (PCL)/Pluronic F127 membranes immobilized with bioactive molecules [vascular endothelial growth factor (VEGF) and/or laminin] were prepared to investigate the effect of musclederived stem cells (MDSCs) on smooth muscle cell (SMC) differentiation. The VEGF and laminin immobilized on the PCL/F127 membrane surface were released with a sustained manner for 28 and 14 days from the membrane, respectively. The SMC differentiation behavior of MDSCs on the membranes immobilized with single or dual bioactive molecules was compared by DNA quantification, RT-PCR, and immunohistochemical analyses. The dual VEGF/laminin-immobilized membrane group showed higher cell growth and more effective SMC differentiation than the single VEGF- or lamininimmobilized group. From our findings, we suggest that the dual VEGF/laminin-immobilized membrane may be applicable to use as a guided smooth muscle regeneration membrane.