http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
전소영 ( So Young Chun ),오세행 ( Se Heang Oh ),유지 ( James J Yoo ),권태균 ( Tae Gyun Kwon ) 한국조직공학과 재생의학회 2015 조직공학과 재생의학 Vol.12 No.1s
Organ transplantation has often been successful for treatment of end-stage organ failure. However, the shortage of donor organ still remains problematic in clinical practices. As an alternative, the tissue-engineering approach for functional organ replacement has been extensively studied. More recently, decellularized organs have been emerged as a promising scaffold for reconstruction of the complicated organs (e.g., heart, liver, lung and kidney). The ideal decellularized organ scaffolds need to contain extracellular matrix (ECM), bioactive molecules, vascular systems and tissue microarchitecture. To fulfill these requirements, physical, chemical, and biological techniques have been adapted in the process of organ decellularization. In this review, the representative techniques for the organ decellularization and their characterization as well as considerations for implantation are discussed.
천연 냉동보존첨가제를 이용한 골수유래 중간엽 줄기세포의 냉동보존
조현정 ( Hyun Jung Cho ),이승희 ( Seung Hee Lee ),서장수 ( Jang Soo Suh ),유지 ( James J Yoo ),손윤희 ( Yun Hee Shon ) 한국조직공학·재생의학회 2013 조직공학과 재생의학 Vol.10 No.2s
Mesencymal stem cells have been used as an “off the shelf” cellular therapeutics due to their immunomodulatory properties. However, cryoprotectants used to store these cells have not been adequately studied, in terms of their safety and efficacy. In this study, we investigated whether use of natural cryoprotectants free of animal products would be effective in the cryopreservation of human bone marrow-derived mesenchymal stem cells (BMSCs). Cryopreservation medium containing disaccharides, antioxidants and caspase inhibitors combined with a reduced concentration of dimethylsulfoxide (DMSO) was used to cryopreserve BMSCs. The cells were tested for viability with cck-8 assay and a growth curve was generated to measure population doubling. In addition, flow cytometry analysis for cell surface antigens, and RT-PCR for mRNA expression of stem cell markers were performed. The solutions containing trehalose and catalase with 5% or 2.5% (v/v) DMSO produced results similar to those for that of the control (10% (v/v) DMSO and 30% FBS), in terms of cell viability, culture growth, expression of cell surface antigens and mRNA expression of stem cell markers in BMSCs cryopreserved for 3 weeks. Thus, these results show that BMSCs can be cryopreserved with a reduced concentration of DMSO with the addition of disaccharides, antioxidants and caspase inhibitors. This study suggests that human BMSCs can be effectively cryopreserved using natural cryoprotectants combined with reduced concentration of DMSO for cell therapy applications.
CD274와 Indoleamine 2,3-Dioxygenase를 통한 양수줄기세포의 면역조절능
이승희 ( Seung Hee Lee ),조현정 ( Hyun Jung Cho ),서장수 ( Jang Soo Suh ),유지 ( James J Yoo ),손윤희 ( Yun Hee Shon ) 한국조직공학·재생의학회 2013 조직공학과 재생의학 Vol.10 No.2s
Abstract :Amniotic fluid-derived stem cells (AFSCs) might be an alternative and less-invasive source of mesenchymal stem cells (MSCs) that could be used in regenerative medicine. AFSCs share the similar immunosuppressive properties with MSCs derived from bone marrow. However, the mechanisms behind the immune responses of AFSCs remain unclear. In this paper, we investigated inhibitory effect of AFSCs on immune cells proliferation induced by mitogen. Measurement of IFN-γ levels in the culture supernatants revealed a significant decrease of the cytokine when mitogen-activated peripheral blood mononuclear cells (PBMCs) were cultured in the presence of AFSCs. AFSCs constitutively expressing HLA-ABC and CD274 increased the expression levels of them with the treatment of IFN-γ. Whereas, HLA-DR, HLA-G and co-stimulatory molecules such as CD80, CD86 were not expressed. Moreover, IFN-γ treatment also induced the expressions of indoleamine 2,3-dioxygenase (IDO), transforming growth factor-β (TGF-β) and IL-10 in AFSCs. Treatment with IDO inhibitor and anti-CD274 reverted the modulatory effect of the AFSCs. From these data, we suggest that AFSCs carry the immunomodulatory characteristics mediated by CD274 and IDO. Findings obtained from this study could be utilized for the development of amniotic fluid-derived stem cell therapy products with the further study.
손미영 ( Mi Yeung Sohn ),서지민 ( Ji Min Seo ),조현정 ( Hyun Jung Cho ),서장수 ( Jang Soo Suh ),성원준 ( Won Joon Seong ),박일수 ( Il Soo Park ),( Anthony Atala ),유지 ( James J Yoo ),손윤희 ( Yun Hee Shon ) 한국조직공학·재생의학회 2012 조직공학과 재생의학 Vol.9 No.1s
Human stem cells are typically obtained for clinical application of cell therapy by isolating and subculturing adherent cells. The expansion of stem cells depends on the culture conditions and requires medium supplemented with fetal bovine serum (FBS) to generate relevant numbers of cells. However, the presence of FBS is a major obstacle for clinical use of the stem cells. Therefore, we have evaluated the capacity of expansion of amniotic fluid-derived stem cells (AFSCs) in a commercial serum-free medium in comparison with a conventional FBS-containing medium. AFSCs were expanded in two different culture media. Proliferation rate of the cells was higher in serum-free medium than in FBS-containing medium. Stem cells isolated in the both media were morphologically similar, and they were expressed identical surface antigens and mRNA of stem cell markers. AFSCs in the both media were also able to differentiate into the myogenic cells. These results suggest that a commercial serum-free medium (MesemCult-XF Medium) is an optimal medium for expansion of AFSCs. It allows a better cell expansion, preserves cell characteristics, and reduces the culture period. This medium seems suitable for clinical scale expansion of AFSCs.