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연제형,최한석,정준휘,고영준,김다연,안시현,안재형,원항연 한국미생물학회 2023 미생물학회지 Vol.59 No.4
Rhizobacter sp. SBD 7-3, a polybutylene adipate terephthalate (PBAT)-degrading strain, was isolated from upland soil that has been installed by biodegradable mulching films in Yeongwol province, Republic of Korea. The genome analysis revealed strain SBD 7-3 genome comprised one chromosome (5,594,180 bp) and two plasmids (200,976 bp; 142,941 bp), with G + C contents of 67.6%, 60.7%, and 61.4% respectively. The genome contained 5,540 protein-encoding genes, 58 tRNA genes, and 3 rRNA genes. Several genes linked to the breakdown of PBAT are found throughout the genome.
미생물제(Rhodococcus sp. 3-2) 처리에 따른 토양 중 카벤다짐의 분해효과
연제형 ( Jehyeong Yeon ),김현수 ( Hyeon-su Kim ),안재형 ( Jae-hyung Ahn ),한귀환 ( Gui Hwan Han ),오영곤 ( Young Goun Oh ),조일규 ( Il Kyu Cho ),박인철 ( In-cheol Park ) 한국환경농학회 2021 한국환경농학회지 Vol.40 No.4
BACKGROUND: The fungicide of benomyl, a benzimidazole group, has been commonly used for pesticides against fungal diseases in the world. However, benomyl is rapidly hydrolyzed in the environment after using to control plant diseases and has adverse effects by generating carbendazim, which is toxic to plants, humans, and the environment. METHODS AND RESULTS: In this study, the decomposition effect of carbendazim, a degradation product of benomyl was conducted in pot and field after making a prototype of benomyl-degrading microbial agent (BDMA). We found that the carbendazim-degrading microbial agent (CDMA) (10<sup>5</sup>, 10<sup>6</sup>, and 10<sup>7</sup> cfu/g soil) decomposed carbendazim by 50% or more in all the treatments, compared to the untreated control in the pot tests after four weeks. The effect of 100% decomposition of carbendazim was observed at 7 days after treatment, when the prototype of BDMA was apllied at 10-folds dilution in the field. The decomposition effect at more than 60% and plant growth promoting effect were observed after 7 days of the treatment, compared with the untreated group in the second field experiment,treated with commercially available concentrations of 500-folds and 1,000-folds. CONCLUSION(S): These results might represent that the BDMA would decompose carbendazim effectively, a decomposition product of the fungicide benomyl, remaining in agricultural area, and it could be utilized practically by using a low dilution rate.
쥐 해마에서 글루타메이트의 시간에 따른 변화가 급성 스트레스 연구에 미치는 영향 : 자기공명분광학을 이용한 연구
연제형(Jehyeong Yeon),윤창수(Chang-Soo Yun),황윤호(Yoonho Hwang),백현만(Hyeon-Man Baek),김동윤(DongYoun Kim),한봉수(Bong Soo Han) 대한전자공학회 2021 대한전자공학회 학술대회 Vol.2021 No.6
It was reported that rodents were significantly influenced by stress from experimental procedure and by isoflurane anesthesia. Therefore, in MRS experiments in the hippocampus of normal mice, the concentrations of Glu and tCho were observed to decrease over time. Glu is a potential stress biomarker and its concentration increases with stress. The purpose of this study is to investigate the effect of the time delay that may occur during 1H-MRS experiments on acute stress studies by comparing the control group and the stress group. 20 C57BL/6N male mice were grouped into the control group and the stress group. The stress group mice were exposed to acute constrain stress, and 1H-MRS data were obtained from the left hippocampus, and the difference in the concentration of metabolites due to stress was compared. We found that the statistical significance of the difference in Glu concentration between the two groups changed if there was a difference of about 17 minutes in the data collection time between the two groups. In conclusion, the results of this study suggest that it is necessary to avoid the difference in the timing of data collection between the control and experimental groups in stress studies.
미생물제(Sphingobium sp. Cam5-1) 처리에 따른 토양 중 카두사포스의 분해효과
연제형 ( Jehyeong Yeon ),정준휘 ( Joon-hui Chung ),최한석 ( Han Suk Choi ),고영준 ( Young-joon Ko ),김다연 ( Dayeon Kim ),안시현 ( Sihyun An ),안재형 ( Jae-hyung Ahn ),한귀환 ( Gui Hwan Han ),원항연 ( Hang-yeon Weon ) 한국환경농학회 2023 한국환경농학회지 Vol.42 No.4
Cadusafos, an organophosphorus insecticide, has been commonly used against various pests worldwide. Organophosphorus pesticides have shorter half-lives and lower toxicities than organochlorine pesticides. However, excessive use of Cadusafos can increase pest resistance and issues with acetylcholine biomagnification, potentially resulting in human toxicity. In this study, we investigated the effect of a Cadusafos-degrading microbial agent (CDMA) prepared using Sphingobium sp. Cam5-1, which was previously reported to effectively degrade residual Cadusafos in soil. Experiments were conducted under both controlled laboratory and greenhouse field conditions. Under laboratory conditions, CDMA (10<sup>6</sup> cfu/g soil application rate) decomposed 97% of Cadusafos in the soil in the untreated control after 21 days. Additionally, when CDMA (10<sup>6</sup> cfu/g soil) was mixed with quicklime, 99% of Cadusafos was decomposed within 3 days. Under greenhouse field conditions, the combined effect of CDMA (10<sup>6</sup> cfu/g soil) and quicklime was not observed. However, CDMA (10<sup>6</sup> cfu/g soil) application alone was capable of decompos- ing 91% of Cadusafos after 3 days. These results indicate that CDMA can effectively decompose high residual levels of Cadusafos in soils under field conditions using a low inoculum rate.
박효정,김재윤,이창묵,안시현,연제형,안재형,김다연,원항연 한국미생물학회 2022 미생물학회지 Vol.58 No.4
Janibacter terrae strain COS04-44, a bacterium that degrades polycaprolactone (PCL), was isolated from crude oil-contaminated tidal flat in Taean, Republic of Korea. Janibacter terrae strain COS04-44 has a genome that is 98.85% identical to that of J. terrae NBRC 107853T and consists of one circular chromosome with 3,394,906 bp. J. terrae COS04-44 has 3,299 protein-coding genes and 58 non-coding RNAs. The G + C content of the genome is 71.9%. These findings provide information on various biodegradable polymer-degrading enzymes and compare differences among the complete genome of the current members of the Janibacter genus.
이제일,박승혜,권용은,Eugeney Oh,김동운,김환욱,연제형,김진철,최준식 한국고분자학회 2021 Macromolecular Research Vol.29 No.9
Nonviral vectors have the potential for gene delivery comparable to viral vectors given their productive and economic advantages. However, the low transfection efficiency and specificity of nonviral vectors limit their application in gene medicine. To increase specificity, we selected cystamine core polyamidoamine (PAMAM) generation 3 (cPAMAM G3), a bioreducible polymer, and synthesized the FKFL sequence, as a cathepsin B-cleavable linker to cPAMAM G3. In addition, we simultaneously introduced RH dipeptides to FKFL-cPAMAM G3 to improve transfection efficiency. The synthesized RHFKFL-cPAMAM G3 showed similar transfection efficiency and low cytotoxicity compared with that of PEI 25 kDa in HepG2 and SW480 cells, which are cathepsin B enzyme overexpression cell lines. These results imply that a combination of enzyme- and reduction-responsive linkers can be used to solve the problems associated with the use of nonviral vectors.
반응표면분석법을 이용한 diazinon 분해 미생물 Paenibacillus tritici PH55-1의 생산 배지 최적화
김예은,송재경,안재형,안시현,원항연,김정준,정준휘,연제형,김다연 한국미생물학회 2022 미생물학회지 Vol.58 No.3
본 연구는 반응표면분석법을 이용하여 Paenibacillus tritici PH55-1 균주의 생장량 증진을 위한 최적 배지 조성과 배양 조건을 탐색하기 위해 수행되었다. PH55-1 균주는 diazinon 분해능이 있는 균주로서, diazinon 생분해와 관련된 유전자를 갖는 미생물로 보고된 바 있다. 총 6개의 배지 조성 및 5개의 배양조건 중 P. tritici PH55-1 균주의 생장에 가장 큰 영향을 미치는주요 인자를 분석하기 위한 방법으로 Plackett-Burman design (PBD)을 설계했다. 실험 결과, 6개의 배지 조성 중 균주의 생장에 영향을 주는 탄소원으로 D-mannitol, 질소원으로 탈지 대두분말(defatted soy flour, DSF) 그리고 미량 원소로 Na2MoO4을최종 선정하였으며, 온도를 5개의 배양 조건 중 배양에 영향을미치는 주 요인으로서 최종 선정하였다. Paenibacillus tritici PH55-1 균주의 배양 최적화를 위한 각 요인들의 최적조건은중심합성계획법(central composite design; CCD)에 기반하였으며, 실험 모델에 의한 최적 조건은 D-mannitol 3.7%, 탈지 대두 분말 5.5%, Na2MoO4 2.2 mM, 온도는 33.8°C으로 예측되었다. 이 때 P. tritici PH55-1 균주의 생장량은 9.67 × 109 CFU/ml 으로 예측되었으며, ANOVA분석을 통해 실험 모델의 적합성과 유의성을 확인하였다. 최종적으로 실험 모델의 검증을 위한 실제 배양 결과, 최적 배양 조건에서 균주 생장량이 실험 모델에 의해 예측된 생장량과 통계적으로 차이가 없음을 확인하였다(p > 0.05). 또한, 최적 배지에서 PH55-1 균주의 생장량이상용 배지인 TSB, LB와 비교하여 각각 13.3배, 54.4배 향상된것을 검증하였다. 본 연구는 diazinon을 분해하는 균주인 P. tritici PH55-1을 산업적으로 이용하여 농경지 토양과 작물에서의 잔류가 문제 되고 있는 diazinon의 분해에 적용할 수 있는연구자료로 유용하게 활용 할 수 있을 것으로 사료된다.