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Methylobacterium extorquens AM1의 메탄올을 이용한 성장과 세포내 폴리아민 구성에 미치는 배양조건의 영향
엄치용,박기정,강빈구,김영민 한국미생물학회 1991 미생물학회지 Vol.29 No.6
Methylobacterium extorquens AM1 growing on methanol as a sole source of carbon and energy was found to grow most rapidly (t$t_{d}$ =6h) at 30.deg.C in a mineral medium (pH 7.0) containing 0.5% (v/v) methanol which was agitated at 200 rpm (optimal cultivation condition). Cells grown under the optimal cultivation condition contained more spermidine, but less putrescine, than the cells grown on 2.5%(v/v) ( $t_{d}$ =8h ) or at 20.deg.C ( $t_{d}$ =8h ). Cells cultivated under the optimal condition was found to contain more spermidine than the cells grown at pH 6.0 (( $t_{d}$ =7h ) and pH 8.0 ($t_{d}$ =7.3h). the cells growing at the stationary phase under the optimal condition accumulated more spermine or putrescine than the cells growing at the same phase on 2.5%(v/v) methanol or at pH 6.0 and pH 8.0, respectively. M. extorquens AM1 grown in a medium agitated at 100 rpm ( $t_{d}$ =8.8h ) contained less spermidine and spermine than the cells grown under the optimal cultivation condition.
상이한 에너지원을 이용하여 성장한 methylobacterium extorquens AM1내의 폴리아민
엄치용,이순희,김영민 한국미생물학회 1990 미생물학회지 Vol.28 No.4
Putrescine, spermidine, and spermine were found to persent in Methylobacterium extorquens AM1 growing on methanol, succinate, glucose, or nutrient broth as an energy source. Spermidine was found to be a major polyamine in cells growing on methanol or succinate, while putrescine to be the one in nutrient broth-grown cells. The overall content of polyamines in cells growing on glucose was less than that in cells growing on other substrates. Spermine was the most abundant polyamine in glucose-grown cells. Accumulation of polyamines in M. extorquens AM1 was maximal at the mid-exponential or early stationary phase during growth on each substrate. The effect of polyamines added into the medium on the polyamine composition in M. extorquens AM1 was variable. Each polyamine added into the nutrient broth medium was found to increase the amount of the respective polyamine in the cell. Exogeneously added polyamines had no effect on the growth of M. extorquens AM1.
Biogenic Nano-Synthesis; towards the Efficient Production of the Biocompatible Gold Nanoparticles
Gajanan Ghodake,엄치용,김시욱,진언선 대한화학회 2010 Bulletin of the Korean Chemical Society Vol.31 No.10
We present a rapid biogenic method for the production of nanoscale gold particles using pear extract. The formation and stability of pear-derived gold nanoparticles (Pear-AuNPs) were monitored by ultraviolet-visible spectroscopy. Their morphology, elemental composition and crystalline phase were determined by transmission electron microscopy, energydispersive X-ray spectroscopy and selected area electron diffraction. The average core size of crystalline Pear-AuNPs was in the range of 10 ± 5 nm and the observed morphology was spherical. The X-ray photoelectron spectrum showed a strong peak for the pure ‘Au’ phase. The circular dichroism spectrum indicated the natural capping ability of the pear extract, which generated peptide-gold nanoparticles. These nanoparticles were stable in aqueous solution for two months. A cell viability assay of Pear-AuNPs showed biocompatibility with human embryonic kidney 293 cells. Accordingly,this eco-friendly process for the bio-mimetic production of Pear-AuNPs is nontoxic in nature; consequently, it will find potential application in nano-biotechnology.
Isolation and Taxonomic Characterization of a Novel Type I Methanotrophic Bacterium
김희곤,한귀환,엄치용,김시욱 한국미생물학회 2008 The journal of microbiology Vol.46 No.1
A methane-oxidizing bacterium was isolated from the effluent of manure and its molecular and biochemical properties were characterized. The isolate was aerobic, Gram-negative, and non-motile. The organism had a type I intracytoplasmic membrane structure and granular inclusion bodies. The outer cell wall surface (S-layers) was tightly packed with cup-shaped structures. Colonies were light yellow on nitrate mineral salt agar medium. In addition, the organism was catalase and oxidase positive. The isolate used the ribulose monophosphate (RuMP) pathway for carbon assimilation, and was able to utilize methane and methanol as a sole carbon and energy source, however, it could not utilize any other organic compounds that were tested. The cells grew well in a mixture of methane and air (methane:air=1:1, v/v) in a compulsory circulation diffusion system, and when grown under those conditions, the optimum pH was approximately 7.0 and the optimal temperature was 30°C. In addition, the specific growth rate and generation time were 0.13 per h and 5.43 h, respectively, when grown under the optimum conditions. The major ubiquinone was Q-8, and the G+C mol% of the DNA was 55.3. Phylogenetic analyses based on the 16S rRNA gene sequence comparisons showed that this bacterium belongs to a group of type I methanotrophs, and that it is most closely related to Methylomicrobium, with a sequence similarity of 99%. Therefore, the isolate was named Methylomicrobium sp. HG-1.
임미나,최시영,이성은,엄치용,윤인선,황용식 한국식물학회 2016 Journal of Plant Biology Vol.59 No.1
An efficient monitoring of cytoplasmic vacuolation by fluorescein diacetate (FDA) staining of protoplasts revealed that the major populations of suspension-cultured rice cells undergo a rapid vacuolation upon glucosedepletion. As aquaporin-family proteins, tonoplast intrinsic proteins (TIPs) are known to play in regulating the water balance across the vacuolar membrane. Glucose starvation increased expression of every member of OsTIP family, leading to an enhancement of the total expression by up to 110-fold, which is well matched with an expansion of the vacuolar structure induced by starvation. OsTIPs 1;1, 2;2 and 3;1 are the three most prominently expressed OsTIPs in starved conditions due to their highest responsiveness to sugar deprivation. Feeding experiments with various sugars and glucose analogs indicated that sugar regulated expression of the three major OsTIPs is likely mediated by a hexokinasedependent pathway. Alleviation of sugar-induced suppression of OsTIP expression by co-treatment with the uncoupler of ATP synthesis suggests that sugar signaling for OsTIP regulation is also cross-talked by the energy-deficit conditions. Intriguingly, starvation-induced central vacuolation was effectively prevented by mannose and 2-deoxyglucose, but not by 3-O-methylglucose. These results imply that the hexokinase is able to trigger the signaling to suppress the central vacuolation, independently of fueling the energy metabolism.
배현정,이하나,백미나,박은진,엄치용,고인정,강호영,오정일 한국분자세포생물학회 2017 Molecules and cells Vol.40 No.9
The DevSR (DosSR) two-component system, which is a major regulatory system involved in oxygen sensing in mycobacteria, plays an im-portant role in hypoxic induction of many genes in mycobacteria. We demonstrated that overex-pression of the kinase domain of Mycobacterium tuberculosis (Mtb) PknB inhibited transcriptional activity of the DevR response regulator in Mycobacterium smegmatis and that this inhibitory effect was exerted through phosphorylation of DevR on Thr180 within its DNA-binding domain. Moreover, the purified kinase domain of Mtb PknB significantly phosphorylated RegX3, NarL, KdpE, TrcR, DosR, and MtrA response regulators of Mtb that contain the Thr residues corresponding to Thr180 of DevR in their DNA-binding domains, implying that transcriptional activities of these response regulators might also be inhibited when the kinase domain of PknB is overexpressed.
Molecular Cloning of a Putative Gene Encoding Phospholipase B (plbA) from Aspergillus nidulans
Hong, SaHyun,Eun-Min Cho,송승은,엄치용 한국미생물·생명공학회 2008 한국미생물·생명공학회지 Vol.36 No.3
The phospholipase B (PLB) families are enzymes sharing phospholipase (PL), lysophospholipase (LPL) and lysophospholipase-transacylase (LPTA) activities. In this study, we report the putative gene encoding phospholipase B (plbA) containing lipase motifs was cloned for the first time from the filamentous fungus,Aspergillus nidulans. plbA was isolated from A. nidulans genomic DNA library using a PCR-amplified probe, which is designed on the basis of sequence information derived from the conserved lipase regions of various PLBs. The deduced product of plbA is of 626 amino acids. From the assigned sequence, PlbA showed 72% identity with Penicillium notatum PLB but have low similarity with phospholipase A of other organisms.