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Microalgal Biotechnology: Carotenoid Production by the Green Algae Dunaliella salina
진언선,Anastasios Melis 한국생물공학회 2003 Biotechnology and Bioprocess Engineering Vol.8 No.6
Unicellular green algae of the genus Dunaliella thrive in extreme environmental conditions such as high salinity, low pH, high irradiance and subzero temperatures. Species of Dunaliella are well known in the alga biotechnological industry and are employed widely for the production of valuable biochemicals, such as carotenoids. Some strains of Dunaliella are cultivated commercially in large outdoor ponds and are harvested to produce dry algal meals, such as polyunsaturated fatty acids and oils for the health food industry, and coloring agents for the food and cosmetic industries. During the past decade, the advances in molecular biology and biochemistry of microalgae, along with the advances in biotechnology of microalgal mass cultivation, enabled this microalga to become a staple of commercial exploitation. In particular, the advent of molecular biology and mutagenesis in Dunaliella has permitted enhancements in the carotenoids content of this green alga, making it more attractive for biotechnological applications. Accordingly, the present review summarizes the recent developments and advances in biotechnology of carotenoid production in Dunaliella.
Biological carbon capture and utilization system using enzymes from microalgae
진언선 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.0
In microalgae, carbonic anhydrase (CA) is an important biocatalyst that captures CO2 and produces bicarbonate. Another biocatalyst is phosphoenolpyruvate carboxylase (PEPCase) to produce C4 chemical, oxaloacetate, from bicarbonate. The development and implementation of strategies for CO2 mitigation technology is necessary to offset the greenhouse gas effect of carbon dioxide emissions. There is the possibility of the capture and utilization of CO2 (CCU) in which waste CO2 is recycled and converted into value added chemicals. To introduce the possibility of bio-based CCU, integrated systems using CA and PEPCase to mimic the in vivo process of carbon dioxide concentration mechanism has been developed in our laboratory. The genetically engineered CA of Dunaliella sp. and PEPCase of Phaeodactylum tricornutum successfully catalyzed a consecutive reaction to generate C4 di-carboxylicacid (OAA). Thus, combined CA-PEPCase specially derived from microalgae could open a possible platform technology for simultaneous CO2 capture and utilization.
박승혜,이유,진언선 한국조류학회I 2013 ALGAE Vol.28 No.2
Dunaliella salina and Dunaliella bardawil are well known for carotenogenesis, the overproduction of carotenoids, under stress conditions. The effect of high light (HL) and low light (LL) on the growth, morphology, photosynthetic efficiency, and the β-carotene and zeaxanthin production of D. salina CCAP 19/18 and D. bardawil was investigated and compared. Both strains showed similar growth kinetics under LL growth condition, but D. salina CCAP 19/18 was faster. As the light intensity increased, D. salina CCAP 19/18 cells were elongated and D. bardawil cells became larger. Both strains showed decrease of the maximum quantum yield of PSII (Fv/Fm) and election transport rate (ETR) under HL growth condition and D. salina CCAP 19/18 was less liable to the light stress. Both strains had about 1.8 and 5 times difference in the O2 evolution rate at LL and HL conditions, respectively. The β-carotene and zeaxanthin production were increased as the light intensity increased in both strains. D. bardawil was more sensitive to light intensity than D. salina CCAP 19/18. The possible application of D. salina CCAP 19/18 as a carotenogenic strain will be discussed.
남온유,Yoshihiro Shiraiwa,진언선 한국조류학회I 2018 ALGAE Vol.33 No.2
Emiliania huxleyi (a haptophyte) is the most abundant coccolithophore species that produces delicate calcite scales called coccoliths. In this study, we identified several candidate genes associated with coccolith production by comparing the transcriptomes of the calcifying (CCMP 371) and non-calcifying (CCMP 2090) strains of E. huxleyi. Among the candidates, genes highly expressed in CCMP 371 were identified. To confirm whether these genes are associated with calcification, we modulated coccolith production in CCMP 371 by culturing it at different calcium concentrations. At an ambient (10 mM) concentration of calcium in the growth medium, CCMP 371 sustained its calcifying ability. However, at a low (0.1 mM) concentration or absence of calcium, there was no calcite formation, demonstrating that calciumlimiting conditions negatively affect calcification. We also evaluated the expression patterns of the putative genes in cells grown at different calcium concentrations by quantitative reverse transcription polymerase chain reaction. In addition, we showed that the growth rate of cells cultured under calcium-limiting conditions does not differ from that under ambient conditions. Further studies are required to investigate the roles of the putative calcification-associated genes at the molecular level.
Biogenic Nano-Synthesis; towards the Efficient Production of the Biocompatible Gold Nanoparticles
Gajanan Ghodake,엄치용,김시욱,진언선 대한화학회 2010 Bulletin of the Korean Chemical Society Vol.31 No.10
We present a rapid biogenic method for the production of nanoscale gold particles using pear extract. The formation and stability of pear-derived gold nanoparticles (Pear-AuNPs) were monitored by ultraviolet-visible spectroscopy. Their morphology, elemental composition and crystalline phase were determined by transmission electron microscopy, energydispersive X-ray spectroscopy and selected area electron diffraction. The average core size of crystalline Pear-AuNPs was in the range of 10 ± 5 nm and the observed morphology was spherical. The X-ray photoelectron spectrum showed a strong peak for the pure ‘Au’ phase. The circular dichroism spectrum indicated the natural capping ability of the pear extract, which generated peptide-gold nanoparticles. These nanoparticles were stable in aqueous solution for two months. A cell viability assay of Pear-AuNPs showed biocompatibility with human embryonic kidney 293 cells. Accordingly,this eco-friendly process for the bio-mimetic production of Pear-AuNPs is nontoxic in nature; consequently, it will find potential application in nano-biotechnology.
김동건,홍성주,배재한,임나래,진언선,이철균 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.4
In this study, a cDNA microarray was developed from over 60,000 mRNA readings to analyze the expression profiles of transcriptomes of Haematococcus lacustris under astaxanthin-inducing culture conditions,high irradiance and nitrate starvation. Among 20,033 genes on the cDNA microarray, 2,675 genes exhibited a twofold or greater difference in expression. Of these, 1,333 genes were up-regulated and 1,342 genes were down-regulated. A significant decrease in the expression of chlorophyll biosynthesis and light harvesting complex (LHC) related genes were observed under astaxanthin inducing conditions (forming red cyst cells). On the other hand, respirationrelated genes, lipid metabolism-related genes and stress response-related genes were activated in the red cyst cells under stress conditions. These results enabled a better understanding of the cell responses during stress induction of H. lacustris such as photosynthesis, respiration, and some biopathways.
Enhanced Production of Biomass and Lipids by Supplying CO2 in Marine Microalga Dunaliella sp.
전한철,이유,장광석,이철균,진언선 한국미생물학회 2013 The journal of microbiology Vol.51 No.6
Non-food-based biofuel feedstocks are in high demand worldwide. Among the various feedstocks, microalgae are the most promising feedstock for mitigating atmospheric CO2 and producing biodiesel. In this study, various concentrations of CO2, from 0.03 to 12%, were used to investigate their effect on the cell growth, biomass and lipid production and fatty acid composition of Dunaliella sp. in a closed photobioreactor. The results showed that the highest biomass and total lipids, 521 mg/L/d and 40 mg/L/d, respectively,were produced with 5% CO2 aeration during the logarithmic growth phase. The oleic acid (18:1n9c) and elaidic acid (18:1n9t) contents were increased approximately two fold. The physiological responses of Dunaliella sp. at 10% CO2 were similar to those at 5% CO2. Therefore, the present results suggest that 5–10% is a suitable CO2 concentration range for Dunaliella sp. growth to mitigate atmospheric CO2 and increase biofuel production.
민병술(Min Byoung-Sul),진언선(Jin Eon-Seon),이미영(Lee Mi-Young) 한국산학기술학회 2004 한국산학기술학회 학술대회 Vol.- No.-
본 연구에서는 미세조류에 광스트레스를 가해서 특정 색소의 증가를 유도하였다. 광스트레스를 미세조류인 N.oculata에 각 각 가했을 때, 안세라크산틴 6.6 배 제아크산틴 8.6 배, 증가함을 HPLC로 분석하였다. 이러한 광스트레스에 노출되면 크산토필 회로에서 비올라산틴이 안세라크산틴과 제아크산틴으로 역위되어 광보호를 하기 때문이다.