http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
안율균,장기창,김천환 (사) 한국생물환경조절학회 2013 생물환경조절학회지 Vol.22 No.1
This study was conducted to investigate the general characteristics of raw okra seeds and the functional componentsof roasted okra seeds. The number of okra seed per pod was 78 in ‘Greensod’ and 88 in ‘Beny’. The weight ofokra seed per pod of ‘Greensod’ and ‘Beny’ were 4.4 g and 6.3 g, respectively. Free amino acid contents of the stir-fryand fresh okra seeds were measured as 2.69 mg · g−1 and 0.31 mg · g−1. Total polyphenolic compound content of the stirfryokra seeds was estimated as 12.61 mg CGA · g−1, compared to 2.54 mg CGA · g−1 fresh okra seeds, Thus, free aminoacid and total polyphenolic compound contents in the stir-fry okra seeds were higher than fresh one, Antioxidant activities,such as DPPH and ABTS radical scavenging in the stir-fry okra seeds was the higher than fresh okra seeds. 본 연구는 오크라 종자를 볶았을 때의 기능성을 성분을 분석하여 용도 다양화를 위한 볶음차로의 이용 가능성을 검토하기 위하여 수행하였다. 꼬투리 당 오크라 종자의 수는 ‘그린소드’ 품종이 78개, ‘베니’ 품종이 88개이었고, 과실 당 종자무게는 각각 4.4g과 6.3g이었다. 볶은 오크라 종자의 유리아미노산 함량은 2.69mg · g−1으로생체종자의 0.31mg · g−1에 비해 8.7배나 높은 함량을 나타내었다. 총 페놀성 화합물 함량은 볶은 오크라 종자가12.61mg CGA로 서 생체 상태보다 5배 높은 함량을 나타내었다. 오크라 종자의 항산화 활성은 DPPH 및ABTS의 경우 볶은 오크라 종자가 생체 상태의 오크라종자 보다 약 2배 이상 월등히 높게 나타내었다.
S-haplotypes and Genetic Diversity in ‘Danji’ Radish (Raphanus sativus L. var. hortensis)
안율균,김혁준,한동엽,박영훈 한국원예학회 2014 원예과학기술지 Vol.32 No.2
The distribution of S-haplotypes and genetic relationships were evaluated for 47 accessions of ‘Danji’ radish (Raphanussativus L. var. hortensis Baker f. gigantissimus Makino) originating from Jeju Island in South Korea. A total of 22 S-haplotype-specificSCAR markers for the S locus glycoprotein (SLG) and S receptor kinase (SRK) loci were tested, and six primer sets amplifiedlocus-specific PCR fragments from at least one ‘Danji’ radish accession. S5 and S21 alleles atthe SLG locus were the most frequentlydistributed, and detected from 87.5% and 64.6% of the accessions, respectively. The frequency of the class-II haplotype at theSLG locus was 75%, more frequent than the class-I haplotype. The S23 allele at the SRK locus was detected from 7 accessions. Grouping of the accessions based on S-allele composition revealed three major groups, while 8 accessions showed a unique alleliccomposition. The genetic diversity of 47 ‘Danji’ radishes and 1 ‘Gwandong’ radish were also evaluated with 38 RAPD primers. A total of 312 bands were scored, and showed that 138 bands (44.2%) were monomorphic among the accessions, whereas 174(55.8%) bands were polymorphic. Polymorphism rates ranged from 0.2 to 1.0, indicating significant variations in detectingpolymorphism across RAPD primers. The genetic similarity coefficients among all pairs of the 48accessions varied from 0.62to 0.93, and 42% of the comparisons exhibited values higher than 0.85. All the cultivars could be distinguished based on theDNA fingerprints revealed by RAPD. The comparisons between the dendrograms based on S-haplotypes and RAPDs indicate anunrelated and sporadic distribution for several accessions; however, there was a tendency for accessions with the same S-alleliccomposition to group into the same cluster.
안율균,김도선,윤무경 한국식물생명공학회 2009 식물생명공학회지 Vol.36 No.4
본 연구는 마늘의 형질전환 체계를 확립하기 위해 캘 러스 형성과 식물체 재분화에 효과적인 방법을 개발하고 자 수행되었다. 가장 적합한 캘러스 형성 배지는 ‘단양 종’과 의성종 마늘 모두 1.0 mg/L 2,4-D 와 0.2 mg/L IAA 였다. 캘러스 형성율은 ‘단양종’ 마늘은 81.2% 였고 의성 종 마늘은 76.1%였다. 캘러스 형성을 유도한지 8주 후 형 성된 캘러스를 재분화 배지에서 7주 동안 배양하였다. 가 장 적합한 식물체 재분화 배지는 ‘단양종’ 마늘의 경우 5 mg/L kinetin와 1 mg/L NAA 였고 ‘의성종’ 마늘의 경우는 10 mg/L BAP였다. 재분화된 식물체는 하우스에 이식하 였는데, 거의 모두가 생존하였다. 기내뿌리를 이용한 식 물체 재분화 체계는 마늘 형질전환을 위해 유용하게 이 용 될 것이다. This study was conducted to develop an effective production of callus induction and plant regeneration system for garlic transformation. The best callus production occurred on in vitro root segment initially cultured on MS medium with 1.0 mg/L 2,4-D and 0.2 mg/L IAA in both ‘Danyang’ and ‘Euseong’. The frequency of callus formation were 81.2% ‘Danyang’ and 76.1% ‘Euseong’. Eight weeks after callus induction, callus lines were transferred to regeneration medium during 7 weeks. The best shoot regeneration medium was MS supplemented with 5 mg/L Kinetin and 1 mg/L NAA for ‘Danyang’ and MS supplemented with 10 mg/L BAP for ‘Euseong’. The frequency of shoot regeneration were 51.5% ‘Danyang’ and 56.6% ‘Euseong’ The plantlets were acclimatized and transferred to the greenhouse with almost survival. This in vitro regeneration system should be useful for garlic transformation.
안율균,전종성,윤무경 한국분자세포생물학회 2013 Molecules and cells Vol.36 No.2
The genetic improvement of garlic plants (Allium sativum L.) with agronomical beneficial traits is rarely achieved due to the lack of an applicable transformation system. Here, we developed an efficient Agrobacterium-mediated transformation procedure with Danyang, an elite Korean garlic cultivar. Examination of sGFP (synthetic green fluorescence protein) expression revealed that treatment with 2-(N-morpholino) ethanesulfonic acid (MES), L-cysteine and/or dithiothreitol (DTT) gives the highest efficiency in transient gene transfer during Agrobacterium co-cultivation with calli derived from the roots of in vitro plantlets. To increase stable transformation efficiency, a two-step selection was employed on the basis of hygromycin resistance and sGFP expression. Of the hygromycin-resistant calli initially produced, only sGFP-expressing calli were subcultured for selection of transgenic calli. Transgenic plantlets produced from these calli were grown to maturity. The transformation efficiency increased up to 10.6% via our optimized procedure. DNA and RNA gel-blot analysis indicated that transgenic garlic plants stably integrated and expres-sed the phosphinothricin acetyltransferase (PAT) gene. A herbicide spraying assay demonstrated that transgenic plants of garlic conferred herbicide resistance, whilst non-transgenic plants and weeds died. These results indicate that our transformation system can be efficiently utilized to produce transgenic garlic plants with agronomic benefits.