농가의 병해충 관리 현황 이해를 위한 설문조사 결과

권덕호,Kwon, D.H. 국립한국농수산대학교 교육개발센터 2021 현장농업연구지 = Journal of practical agricultural resear Vol.23 No.2

한국농수산대학 재학생 및 졸업생 151명을 대상으로 병해충 관리 현황을 조사하기 위해 설문조사를 수행하였다. 설문 내용은 기본문항과 병해충 관리 문항으로 구성되어 있다. 기본문항에는 응답자의 연령, 학적상태, 재배작물, 재배면적등을 포함하고 있으며, 병해충 관리 문항에는 병해충 방제 방법, 농약 선택 근거, 병해충 예찰 방법 등을 포함하고 있다. 기본문항의 응답을 요약하면 전체 응답자 중에서 20대 응답자가 91.2%로 가장 높았고, 응답자의 재배 면적은 3헥터 이상이 34.5%로 가장 높은 비중을 차지하고 있었다. 병해충 관리 문항의 응답을 요약하면 병해충 관리 방식에서는 화학적 방제법이 66%로 가장 높은 비중을 차지하였다. 농약 선택 근거에서는 본인 스스로 기존의 방제 기술을 토대로 결정(30%)하거나 농약 판매상(29%)의 결정을 따랐다. 병해충 예찰 방법은 농촌진흥청 소속기관(29%)과 국가농작물병해충관리시스템(27%)을 활용하는 것으로 나타났다. 농약 판매상의 병해충 진단과 농약 처방에 대한 질문에 응답자의 97%가 보통 이상의 신뢰도를 보였으나 강한 신뢰도를 선택한 경우는 전혀 없었다. 병해충 진단 및 농약 처방을 위한 전문인력 양성 필요성에 대해서는 응답자의 79%가 높은 필요성이 있는 것으로 응답하였는데, 특히, 응답자의 47%는 매우 강한 필요성이 있다고 응답하였다. 이러한 결과는, 병해충 진단과 농약 처방을 위한 정교한 기술을 지닌 병해충 관리를 위한 전문인력이 농업 현장에 필요함을 의미한다. 농민의 시각에서 조사한 설문 결과는 병해충 관리 현황을 이해하고 향후 발전 방향을 제시하는 데 중요한 정보를 제공해 줄 것이다. To investigate the current pest management status in Korea, a survey was conducted from 151 students and graduates in the Korea National College of Agriculture and Fisheries (KNCAF) by on-line. The questionnaire consists of two divisions, basic questions and pest control questions. The basic questions were including the respondent's age, academic status, cultivating crops and cultivating area. The pest control questions were including pest control methods, pesticide selection rationale, and pest forecasting methods. As a summary of basic questions, the respondents in their 20s accounted for 91.2%. Moreover, 34.5% of the respondents had over 3 hectares of cultivating area. The cultivating methods were differed by cultivating crops. As a summary of pest control questions, major control methods were using the conventional chemicals (>66%). To understand the pesticide selection rationale, farmers/respondents made their own decisions based on existing control techniques (30%) or depended on the decisions of pesticide vendors (29%). As for the pest forecasting method, it was mainly conducted by the Rural Development Administration affiliated organization (29%) and the National Crop Pest Management System (27%). Regarding the reliability of the pest diagnosis and pesticide prescription of pesticide vendors, 97% of the respondents marked above average. However, there was no choice on strong reliability. Interestingly, 79% of the respondents agreed to train experts for pest diagnosis and pesticide prescription with high necessity and, in particular, 47% of respondents were very strongly supported. These results suggest that the farmers might be need more qualified experts in pest diagnosis and pesticide prescriptions. Taken together, these survey results would provide important information to understand the current status of pest management by farmers' point of view and useful to set the direction of pest control.

가루이류의 충해 관리용 농자재에 대한 약효 반응

권덕호,권민정,유기렬,박준현,김선형,안율균 한국농약과학회 2023 한국농약과학회 학술발표대회 논문집 Vol.2023 No.11

Genetic diversity and structure in apple-infesting pests of Carposina sasakii, Grapholita dimorpha and Grapholita molesta in Korea

권덕호,김동환,김형환,이시혁,양창열 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.1

The genetic diversity of three apple-infesting pests, Carposina sasakii, Grapholita dimorpha and Grapholitamolesta, found in four local regions in Korea,was investigated using the mitochondrial cytochrome c oxidase I (COI) gene as amarker. Six, seven, and three larval haplotypes fromC. sasakii, G. dimorpha, and G. molesta, respectively,were observed. The haplotype and nucleotide diversitywere ranged as 0.31–0.69 and 0.0006–0.0040, respectively. The estimates of genetic differentiation (FST) among local populations in C. sasakii and G. dimorpha exhibited very narrowrange differentiation as 0 to 0.025 and 0 to 0.075, respectively,whereas G. molesta revealed amoderate range of genetic distance (FST=0–0.461). In the AMOVA analysis, low percent variations (0–0.98%) and FST values (0 and 0.01, respectively) observed in C. sasakii and G. dimorpha, respectively,whereas G. molesta revealedmoderate diversity as 31.3% (FST=0.313, p=0.038). These results would provide the basic information to understand the difference of genetic structure in species level and further be utilized to estimate the temporal and spatial distribution of these species in apple orchard.

Plasma-based organism evaluation equipment using atmospheric-pressure plasma jets: Efficacy for controlling insect pests

권덕호,김현승,박미리 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.3

Alternative insect pest control methods are necessary for efficient pest management with reduced dependency on pesticides. Here, we report the biological responses of several insect species to, and the insecticidal efficacy of, reactive oxygen species (ROS) generated using atmospheric-pressure plasma jets. Plasma-based organism evaluation equipment (PBOEE) consisting of a plasma-generating acrylic chamber, plasma-maintaining acrylic chamber, and plasma efficacy evaluation container was developed. The PBOEE system enabled determination of the insecticidal efficacy of ROS free from the adverse effects of high temperature; moreover, four different exposures could be achieved within one run. The biological responses of five major insect pests (Aphis gossypii, Bemisia tabaci, Helicoverpa armigera, Tetranychus kanzawai, and Thrips palmi) were assessed, with a focus on knockdown time, recovery time, and median lethal time (LT 50 ). With short-term exposure (< 3 min), B. tabaci showed the fastest knockdown time (38.4 ± 2.7 s) and the slowest recovery time (699 ± 133 s), and no mortality was noted in any of the five species. On the other hand, with long-term exposure (< 21 min), insecticidal efficacy was observed in B. tabaci and T. palmi, which showed LT 50 values of 6.3 and 9.6 min, respectively. The PBOEE system can be used to determine the optimal exposure time for evaluating the insecticidal efficacy of plasma against insect pests, and plasma can be used for future control of some insect pests.

Xylose Fermentation Was Improved by Kluyveromyces marxianus KHM89 through Up-regulation of Nicotinamide Adenine Dinucleotide (NAD+) Salvage Pathway

권덕호,하석진 한국생물공학회 2022 Biotechnology and Bioprocess Engineering Vol.27 No.4

Xylose fermentation has been reported to be improved in Kluyveromyces marxianus via strain improvement by overexpressing xylose reductase (XYL1) and xylitol dehydrogenase (XYL2). This study performed directed evolution to further enhance xylose consumption in a K. marxianus mutant following transcriptomic analysis to determine genes associated with enhanced characteristics. KmXYL1 and KmXYL2 genes were overexpressed in K. marxianus 17555ΔURA3 for improving xylose fermentation. By performing directed evolution, a mutant K. marxianus KHM89 showing enhanced ethanol production was isolated from xylose medium. K. marxianus KHM89 consumed 47.39 g/L of xylose and produced 22.62 g/L of xylitol and 10.59 g/L of ethanol while the parental strain consumed 25.15 g/L of xylose and produced 7.36 g/L of xylitol and 2.05 g/L of ethanol. RNA sequencing-based transcriptomic analysis showed that alcohol dehydrogenases, aldehyde dehydrogenases, and NAD+ salvage pathway enzymes were upregulated in K. marxianus KHM89. These results were achieved via a combinatorial approach of rational design and directed evolution. The findings of this study contribute to the improvement of xylose fermentation by K. marxianus at an industrial scale.

채소 육묘장에서 발생하는 충해 진단 및 방제를 위한 인공지능 학습 데이터 수집 현황

권덕호,유기렬,신범수,안율균 한국농약과학회 2023 한국농약과학회 학술발표대회 논문집 Vol.2023 No.11

딸기 시설 하우스 점박이응애에 대한 살비 효과 평가

권덕호,안율균,홍규현,Kwon, D.H.,Ahn, Y.K.,Hong, K.H. 국립한국농수산대학교 교육개발센터 2020 현장농업연구지 = Journal of practical agricultural resear Vol.22 No.2

딸기 재배지에서 점박이응애는 엽록소를 직접적으로 흡즙하여 생산량을 낮추는 주요 난방제 해충이다. 본 연구에서는 간이 약효 검정법을 개발하여 친환경유기농자재와 화학합성살비제가 포함된 10종의 약효를 평가하였다. 해당 기법은 4개의 약제를 3반복으로 실험할 수 있는 장점이 있어 시료 전처리 시간을 단축시킬 수 있다. 감수성 계통(SL_YS)을 포함한 전북 정읍(2020_JB_01), 충북 청주(2020_CB_01), 강원 고성(2020_GW_01)에서 채집한 지역 계통을 대상으로 약효를 평가한 결과 감수성 계통은 약 90-100% 사충률을 보인 반면에 지역 계통은 약제별로 상이하게 나타났다. 우수 약제를 선발하기 위해 약효 지수를 감수성 계통을 기준으로 산출하였다. 2020_JB_01 계통에 대한 우수 약제는 Test_02, Test_03 그리고 Test_10 약제가 선정되었다. 2020_CB_01 계통에 대해서는 Test_03과 Test_04가 선정되었다. 2020_GW_01 계통에서는 Test_01, Test_02, Test_03, Test_06 그리고 Test_10이 선정되었다. 계통 별 약제 반응이 다른 이유는 농가별 사용 약제와 살포 횟수가 다르기 때문인 것으로 사료된다. 약효 간이 검정법은 우수 약제 선발을 가능하게 하여 딸기 재배지 점박이응애 방제 효율을 증대시킬 것으로 기대된다. In the strawberry cultivation areas and domestic farms, two-spotted spider mites are the most serious pests. It decreases the product yield due to the direct feeding of chlorophyll by the mites resulting in reduced photosynthetic ability of host plant. In this study, a simplified acaricidal effect evaluation system (SAEES) was employed to choose the effective acaricidal products among 10 items based on leaf-dipping bioassay methods. SAEES had the advantage of being able to screen four commercial products with three replications at the same time in the recommended concentration. The susceptible strains (SL_YS) showed a high mortality rate of about 90-100%, whereas the mortality of local strains was differed by each acaricides. It suggests that the acaricide responses of field populations might differ due to spray frequencies and acaricide product. An efficacy index (0.8-1.0) was determined based on the mortality of susceptible strain, which would allow the most effective commercial products to be selected by the range of this index. In summary, SAEES will enable the selection of effective commercial products and contribute to increasing control against T. urticae in strawberries.

담배가루이 유인용 LED 선발과 기주식물이 초기 유인력에 미치는 영향

권덕호,권민정,양다영,안율균,홍규현,박미리,Kwon, D.H.,Kwon, M.J.,Yang, D.Y.,Ahn, Y.K.,Hong, K.H.,Park, M.R. 국립한국농수산대학교 교육개발센터 2020 현장농업연구지 = Journal of practical agricultural resear Vol.22 No.2

시설 재배지 내 담배가루이의 밀도 예찰을 위하여 여러 종류의 LED등(청색등, 녹색등, 적색등, 백색등)에 대한 유인력을 평가하였다. 유인력 평가는 황색끈끈이트랩에 부착된 담배가루이의 포집 수를 대상으로 하였다. 평가용 케이지 내에서 기주식물이 없는 조건에서 유인력은 청색등(107.3±2.5마리) > 백색등(83.0±12.1마리) > 적색등(58±21.8마리) > 녹색등(39.7±8.1마리) 순서로 나타났다. 먹이를 주입한 상태에서 유인력은 청색등(52±17.4) > 적색등(38.7±5.8) > 녹색등(12.7±1.5) > 백색등(11.7±5.0) 순서로 관찰되었다. 두 실험 조건에서 모두 청색등에서 유인력이 가장 높은 것으로 나타났다. 기주식물을 넣었을 때 LED등 유인력이 감소하는 것을 확인하였는데, 백색등(85.9%) > 녹색등(68.1%) > 청색등(51.6%) > 적색등(33.3%) 순서로 나타나 기주식물이 LED 등 유인력 감소에 영향이 있음을 확인하였다. 상대적인 방제력 평가에서는 기주식물을 처리한 6시간 이후에 적색등(66.7%) > 청색등(48.5%) > 녹색등(31.9%) > 백색등(14.1%) 순으로 높았다(F_3,8 = 14.7, P = 0.001). 따라서 청색등은 초기 유인력이 매우 높은 특징을 보였고, 적색등은 기주식물의 투입에 영향을 받지 않아 담배가루이 유인에 적합성이 있음을 확인하였다. 야외 실증실험에서는 낮은 온도조건에 의해 높은 유인력은 관찰되지 않았지만, 청색등과 적색등에서 대조구 대비 높은 유인력이 관찰되었다. 향후 적색과 청색을 활용한 LED등은 시설재배지 내 담배가루이의 예찰에 활용할 수 있을 것으로 기대된다. Four LEDs (blue, green, red, and white light) were tested to identify the most attractive wave length to utilize as the forecasting tools for the B. tabaci in glass houses. Attractiveness was evaluated by the total number of the B. tabaci attached to a yellow sticky trap. In the condition of no host plant supplement, the attraction efficacy was ordered from high to low as blue light (107.3±2.5), white light (83.0±12.1), red light (58±21.8), and green light (39.7±8.1). In the supplement of the host plant, the attraction was observed in the order of blue light (52±17.4), red light (38.7±5.8), green light (12.7±1.5), and white light (11.7±5.0). In both experimental conditions, blue light showed the highest attraction. In terms of the host plant effect to LED attraction, it varied following as white light (85.9%), green light (68.1%), blue light (51.6%), and red light (33.3%). This result suggests that red light is the least affected by the host plant. In the evaluation of the relative control efficacy, it was determined following as red light (66.7%), blue light (48.5%), green light (31.9%) and white light (14.1%) (F_3,8 = 14.7, P = 0.001). Taken together, blue light had a very high initial attraction, and red light was revealed low attraction effect by the supplement of the host plant. In field demonstration experiments, a high attractive efficacy was not observed due to low-temperature conditions, but similar higher attractive efficacy was observed in blue and red lights compared to the control. The commercialization of LEDs using red and blue in the future is expected to provide important information regarding B. tabaci population density forecast in glass house.

농약 사용자 관련 해외 법 소개

권덕호,유기렬,안율균 한국농약과학회 2023 한국농약과학회 학술발표대회 논문집 Vol.2023 No.4

F331H mutation and reduced mRNA in type-1 acetylcholinesterase is associated with carbofuran resistance development in the small brown planthopper (Laodelphax striatellus Fallén)

권덕호,김형범,정인홍,이시혁 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.1

Molecular diagnostic markers are necessary for establishing high-throughput screening systems to support insecticide-resistant population management. Here, we identified single amino acid substitution mutations related to carbamate resistance in Laodelphax striatellus Fallén type-1 acetylcholinesterase (LsAChE1) using carbofuranselected strains. The phenotypic resistance profiles of the final selection strain (SEL9) compared to the susceptible strain revealed a 14-fold higher resistance ratio based on topical application, 1.2-fold higher general esterase activity, and 4.3-fold higher acetylcholinesterase insensitivity based on the 50% inhibitory concentration (I 50 ), suggesting that insensitivity of the target site could occur as a resistance factor. Comparison of the nucleotide sequences of Lsace1 of five strains (SUS, SEL0, SEL3, SEL6, and SEL9) revealed two amino acid substitutions (F330Y and F331H). To understand the roles of these mutations, we determined the allele frequency of both point mutations in the selected strains using quantitative sequencing methods. In addition, several quantitative genotypic traits (e.g., gene copy numbers and transcript levels of Lsace1, Lsace2, and LS.CarE1) were assessed. A correlation analysis of genotypic and phenotypic traits revealed strong correlations between resistance level and I 50 with F331H allele frequency. Interestingly, the F331H mutation was negatively correlated with transcript levels of Lsace1, suggesting that selection pressure might result in a reduction of the target gene. Overall, the F331H mutation and reduced mRNA are important factors in the development of carbamate resistance. Furthermore, the point mutation can be used to monitor rapid carbofuran resistance in conjunction with molecular diagnostic methods such as quantitative sequencing.