Inductions of Caspase-, MAPK- and ROS-dependent Apoptosis and Chemotherapeutic Effects Caused by an Ethanol Extract of Scutellaria barbata D. Don in Human Gastric Adenocarcinoma Cells

심지환,김희진,이수진,김병주 대한약침학회 2016 Journal of pharmacopuncture Vol.19 No.2

Objectives: The crude extracts of Scutellaria barbata D. Don (SB) have traditionally demonstrated inhibitory effects on numerous human cancers both in vitro and in vivo. Gastric cancer is one of the most common types of cancer on world. The authors investigated the effects of an ethanol extract of Scutellaria barbata D. Don (ESB) on the growth and survival of MKN-45 cells (a human gastric adenocarcinoma cell line). Methods: The MKN-45 cells were treated with different concentrations of ESB, and cell death was examined using an MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay. Analyses of sub-G1 peaks, caspase-3 and -9 activities, and mitochondrial membrane depolarizations were conducted to determine the anti-cancer effects of SB on MKN-45 cells. Also, intracellular reactive oxygen species (ROS) generation was investigated. Results: ESB inhibited the growth of MKN-45 cells, caused cell cycle arrest, and increased the sub-G1 population. In addition, ESB markedly increased mitochondrial membrane depolarization and the activities of caspase-3 and -9. ESB exerted anti-proliferative effects on MKN-45 cells by modulating the mitogen-activated protein kinase (MAPK) signaling pathway and by increasing the generation of ROS. Furthermore, combinations of anti-cancer drugs plus ESB suppressed cell growth more than treatments with an agent or ESB, and this was especially true for cisplatin, etoposide, and doxorubicin. Conclusion: ESB has a dose-dependent cytotoxic effect on MKN-45 cells and this is closely associated with the induction of apoptosis. ESB-induced apoptosis is mediated by mitochondria- , caspase- and MAPK dependent pathways. In addition, ESB enhances ROS generation and increases the chemosensitivity of MKN-45 cells. These results suggest that treatment with ESB can inhibit the proliferation and promote the apoptosis of human gastric adenocarcinoma cells by modulating the caspase-, MAPK- and ROS-dependent pathway.

인체 위암세포주에서 半枝蓮의 항암 효능에 관한 연구

심지환(Ji Hwan Shim),이수진(Soojin Lee),김희진(Huijin Gim),박현수(Hyun Soo Park),김병주(Byung Joo Kim) 한의병리학회 2015 동의생리병리학회지 Vol.29 No.2

iGLAD를 통한 국내 보행자 교통사고 유형 분석

심지환(Jihwan Sim),이화수(Hwasoo Lee),임종현(Jonghyun Yim),이지민(Jimin Lee),송봉섭(Bongsob Song) 한국자동차공학회 2017 한국자동차공학회 부문종합 학술대회 Vol.2017 No.5

능동안전장치의 소비자 인식 연구

심지환(Jihwan Sim),이화수(Hwasoo Lee),임종현(Jonghyun Yim) 한국자동차안전학회 2016 자동차안전학회지 Vol.8 No.2

Airbnb 숙소 유형에 따른 호스트의 자기소개 텍스트가 공유성과에 미치는 영향

심지환 ( Sim Ji Hwan ),김소영 ( Kim So Young ),정여진 ( Chung Yeojin ) 한국지식경영학회 2020 지식경영연구 Vol.21 No.4

최근 빠르게 성장하고 있는 숙박 공유경제 시장에서 품질에 대한 불확실성은 사용자의 만족도에 영향을 미치는 위험 요소지만, 이는 시설 제공자가 공개하는 정보를 통해 완화될 수 있다. 그 중 시설 제공자의 본인에 대한 자기소개는 사용자와의 정서적 교류를 통해 심리적 거리를 제거함으로써 공유 성과에 긍정적 영향을 미친다. 본 연구는 대표적인 숙박 공유경제 플랫폼인 Airbnb에서 호스트의 자기소개가 포함하는 정보의 종류에 따라 공유성과에 미치는 영향을 분석하고, Airbnb의 숙소 유형에 따라 차이를 분석하였다. 이를 위해 호스트가 공개하는 자기소개 텍스트를 문장별로 분리하고 비지도 학습기반의 딥러닝 방법인 Attention-Based Aspect Extraction 방법을 활용하여 각 문장이 포함하는 의미를 추출하였다. 추출된 의미를 토대로 자기소개 텍스트가 포함하는 의미가 공유성과에 미치는 영향과 숙소 유형에 따른 교호작용 효과를 분석하였다. 연구결과, 숙소 유형별로 호스트의 특정 성향이 공유성과에 긍정적인 영향을 미치는 것을 확인하였고, 이를 통해 숙소 유형에 따라 공유성과를 극대화하기 위한 마케팅 전략에 대한 실증적인 함의를 제공한다. In accommodation sharing economy, customers take a risk of uncertainty about product quality, which is an important factor affecting users' satisfaction. This risk can be lowered by the information disclosed by the facility provider. Self-presentation of the hosts can make a positive effect on listing performance by eliminating psychological distance through emotional interaction with users. This paper analyzed the self-presentation text provided by Airbnb hosts and found key aspects in the text. In order to extract the aspects from the text, host descriptions were separated into sentences and applied the Attention-Based Aspect Extraction method, an unsupervised neural attention model. Then, we investigated the relationship between aspects in the host description and the listing performance via linear regression models. In order to compare their impact between the three facility types(Entire home/apt, Private rooms, and Shared rooms), the interaction effects between the facility types and the aspect summaries were included in the model. We found that specific aspects had positive effects on the performance for each facility type, and provided implication on the marketing strategy to maximize the performance of the shared economy.

구인공고에 나타난 숙련수요 : IT 직종을 중심으로

장지연,심지환 한국노동연구원 2023 노동리뷰 Vol.225 No.-

전문대학 학생의 학업중단 예측에 관한 연구 : 초기 학업 성적의 중요성

오상조,심지환 대한산업경영학회 2024 산업융합연구 Vol.22 No.2

본 연구에서는 서울 소재 한 전문대학 학생들을 대상으로 하여 최소한의 인구통계학적 변수와 1학년 1학기 성적을 활용하여 학생들의 최종 학적 상태를 예측하고자 하였다. XGBoost와 LightGBM 모델을 사용한 결과, 이러한 변수들이 학생 들의 제적 여부 예측에 유의미한 것을 발견하였다. 이는 학업 시작 초기의 성적이 학업 중단의 중요한 지표가 될 수 있음을 시 사한다. 또한, 전문대학의 학제가 최종 학적에 영향을 미칠 가능성을 확인하였으며, 이는 학업 기간이 학생들의 학업 중단 결 정에 중요한 요소임을 나타낸다. 전문대학에서 조기 학업 중단 의도를 파악하는 데 있어 심리적, 사회적, 경제적 요인에 의존 하지 않고 학업 성취도만을 기준으로 모델링을 시도하였다. 이는 향후 학업 중단에 대한 조기 경보 시스템 구축에 도움이 될 것으로 기대된다. This study utilized minimum number of demographic variables and first-semester GPA of students to predict the final academic status of students at a vocational college in Seoul. The results from XGBoost and LightGBM models revealed that these variables significantly impacted the prediction of students' dismissal. This suggests that early academic performance could be an important indicator of potential academic dropout. Additionally, the possibility that academic years required to award an associate degree at the vocational college could influence the final academic status was confirmed, indicating that the duration of study is a crucial factor in students' decisions to discontinue their studies. The study attempted to model without relying on psychological, social, or economic factors, focusing solely on academic achievement. This is expected to aid in the development of an early warning system for preventing academic dropout in the future.

Ethanol Extract of Oldenlandia diffusa – an Effective Chemotherapeutic for the Treatment of Colorectal Cancer in Humans -Anti-Cancer Effects of Oldenlandia diffusa-

이수진,심지환,김희진,박현수,김병주 대한약침학회 2016 Journal of pharmacopuncture Vol.19 No.1

Objectives: Oldenlandia diffusa is traditionally used to relieve the symptoms of and to treat various diseases, but its anti-cancer activity has not been well studied. In the present study, the authors investigated the anti-cancer effects of an ethanol extract of Oldenlandia diffusa (EOD) on HT-29 human adenocarcinoma cells. Methods: Cells were treated with different concentrations of an EOD, and cell death was assessed by using a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Analyses of the sub G1 peak, the caspase-3 and -9 activities, and the mitochondrial membrane depolarizations were conducted to confirm cell death by apoptosis. Also, intracellular reactive oxygen species (ROS) generation was determined using carboxy-H2DCFDA (5-(and-6)-carboxy-20,70-dichlorodihydrofluorescein diacetate). Results: EOD inhibited the proliferation of HT-29 cells for 24 hours by 78.6% ± 8.1% at 50 μg/mL, 74.4% ± 4.6% at 100 μg/mL, 65.9% ± 5.2% at 200 μg/mL, 51.4% ± 6.2% at 300 μg/mL, and by 41.7% ± 8.9% at 400 μg/mL, and treatment for 72 hours reduced the proliferation at the corresponding concentrations by 43.3% ± 8.8%, 24.3 ± 5.1 mV, 13.5 ± 3.2 mV, 6.5 ± 2.3 mV, and by 2.6 ± 2.3 mV. EOD increased the number of cells in the sub-G1 peak in a dose-dependent manner. The mitochondrial membrane depolarization was elevated by EOD. Also, caspase activities were dose-dependently elevated in the presence of EOD, and these activities were repressed by a pan-caspase inhibitor (zVAD-fmk). The ROS generation was significantly increased by EOD and N-acetyl-L-cysteine (NAC; a ROS scavenger) remarkably abolished EOD-induced cell death. In addition, a combination of sub-optimal doses of EOD and chemotherapeutic agents noticeably suppressed the growth of HT-29 cancer cells. Conclusion: These results indicate that EOD might be an effective chemotherapeutic for the treatment of human colorectal cancer.

제한속도정보제공장치(SLIF)에 대한 한국 환경 평가 분석

이화수,심지환,임종현,이홍국,장경진,유송민,Lee, Hwasoo,Sim, Jihwan,Yim, Jonghyun,Lee, Hongguk,Chang, Kyungjin,Yoo, Songmin 한국자동차안전학회 2015 자동차안전학회지 Vol.7 No.4

Exceeding the speed limit during vehicle driving is a key factor in the severity of lots of road accidents, and SLIF(Speed Limit Information Function) application is in the initial phase in Korea. SLIF helps the drivers to observe a speed limit when they are driving by providing alert and informing the current limit speed information based on external data using camera and/or digital map, for that reason, environmental conditions could be causes of SLIF malfunctions. In this study, design adequacy analysis of SLIF in respect of false recognition as the Korea traffic environment has been performed. As tentative results, road conditions and structure of speed limit sign as well as system performance often caused misrecognition.

숙련분류체계 구축을 위한 시론 : IT 직종을 중심으로

백승렬,장지연,심지환 한국노동연구원 2023 노동리뷰 Vol.225 No.-