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신병섭,김휘곤,최욱환 대한폐경학회 2014 대한폐경학회지 Vol.20 No.1
To examine the effect of diazoxide on hypoxia-induced soluble fms-like tyrosin kinase-1 (sFlt-1) release in JEG-3 choriocarcinoma cells.Methods: Cells were cultured under normoxia (20% O2) or hypoxia (1% O2), and expression of sFlt-1 mRNA and protein release was determined by quantitative real-time reverse-transcriptase polymerase chain reaction (qRT-PCR) assays and enzyme-linked immunosorbent assay (ELISA). Results: Tumor necrosis factor-alpha (TNF-α) as well as hypoxia stimulated sFlt-1 release and diazoxide inhibited both of them. The selective inhibitor of mitochondrial adenosine triphosphat (ATP)-sensitive K+ channel opener (KATP) 5-hydroxydecanoate (5-HD) completely reversed the diazoxide-induced inhibition of hypoxia-stimulated sFlt-1 release. qRT-PCR and Western blot analyses showed that diazoxide up-regulated the heme oxygenase-1 (HO-1) expression. In addition, the HO-1 inducer cobalt protoporphyrin (CoPP) and the metabolic product of HO-1 bilirubin mimicked diazoxide to inhibit sFlt-1 release and reactive oxygen species (ROS) production under hypoxia, whereas the HO-1 inhibitor zinc protoporphyrin IX (ZnPP IX) antagonized the effect of diazoxide. In cells transfected with the HO-1 siRNA, diazoxide did not exert any effect on sFlt-1 release and ROS production under hypoxia.Conclusion: These results, taken together, strongly suggest that up-regulation of the HO-1 expression is the crucial mechanism responsible for the diazoxide-induced inhibition of the sFlt-1 release and ROS production under hypoxia. (J Menopausal Med 2014;20:21-31)
자궁내막암에서 CD44v6의 발현과 임상병리학적 예후인자와의 상관성
신병섭 ( Byung Sub Shin ),정재혁 ( Jae Heok Jeong ),정은주 ( En Ju Jeong ),조무성 ( Mu Sung Joo ),박승섭 ( Seung Seop Park ),김기형 ( Ki Hyung Kim ),윤만수 ( Man Soo Yoon ) 대한산부인과학회 2004 Obstetrics & Gynecology Science Vol.47 No.9
목적 : 병리조직검사로 확진된 자궁내막암 환자에서 CD44v6의 임상적 유용성을 알아보고자 본 연구를 시행하였다. 연구 방법 : 전자궁적출술과 양측 자궁부속기 절제술이 시행된 39예의 자궁내막암환자를 대상으로 의무기록지 검토와 면역조직화학 염색을 통한 후향적 연구를 하였다. 결과 : CD44v6 발현은 자궁내막암이 자궁근층 침범 없이 자궁내막에 국한된 침범을 나타낸 경우에 통계학적으로 유의한 차이로 발현되었고, CD44v6 발현된 경우 유의한 차이로 림 Objective : The purpose of this study was to evaluate the clinical usefulness of CD44v6 in patients with histologically confirmed endometrial cancer. Methods : Immunohistochemistry was used to determine the expression of CD44v6 in 39 hysterectomy specimen