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Airway Smooth Muscle에 미치는 자울의 효과
나경상,권의광,소응향,서은미,한종현,Na Kyung-Sang,Kwon Eui-Kwang,Soo Yeung-Hyung,Suh Eun-Mi,Han Jong-Hyun 경락경혈학회 2001 대한침구의학회지 Vol.18 No.1
Radix Asteris has been used in Korea for many centuries as a treatment for respiratory disease. The effect of Radix Asteris on tracheal smooth muscle is not known. The purpose of the present study is to determine the effect of Radix Asteris on histamine induced tracheal smooth muscle contraction in rats and guinea pigs. Guinea pig(500g, male) and Sprague Dawley rats (250g, male) were killed by $CO_2$ exposure and a segment (8-10mm) of the thoracic trachea from each rat and guinea pig was cut into equal segments and mounted 'in pairs' in a tissue bath. Contractile force was measured with force displacement transducers under 0.5g loading tension. The dose of histamine (His) which evoked 50% of maximal response ($ED_{50}$) was obtained from cumulative dose response curves for histamine $(10^{-7}{\sim}10^{-4}M)$. Contractions evoked by His ($ED_{50}$) were inhibited significantly by Radix Asteris. In guinea pig tracheal smooth muscle, the mean percent inhibition of histamine induced contraction was 120.5% (p<0.01) after $100{\mu}l/ml$ Radix Asteris. In rat tracheal smooth muscle, the mean percent inhibition of histamine induced contraction was 135.4% (p<0.01) after $100{\mu}l/ml$ Radix Asteris. Propranolol $(10^{-7}M)$ slightly but significantly attenuated the inhibitory effects of Radix Asteris. Following treatment with propranolol, the mean percent inhibition caused by $100{\mu}l/ml$ Radix Asteris fell to 44.6% in guinea pig induced by histamine contraction and by $100{\mu}l/ml$ Radix Asteris fell to 18.7% (p<0.05) in rat induced by histamine contraction. Indomethacin and methylene blue$(10^{-7}M)$ did not significantly alter the inhibitory effect of Radix Asteris. These results indicate that Radix Asteris can relax histamine induced contraction of guinea pig and rat tracheal smooth muscle, and that this inhibition involves sympathetic effects.
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황경택,경은호,나한일,소응향,최병화,한종현 한국전통의학연구소 2003 한국전통의학지 Vol.13 No.1
The purpose of this study is to measure the changes of regional cerebral blood flow (rCBF) and blood pressure (BP) in rats, following the intravenous injection of Catalpa Ovata Water Extract (COWE). The measurement was continually monitored by laser-doppler flowmeter (Transonic Instrument, USA) and pressure tranducer(Grass, USA) in anesthetized adult Sprague-Dawley rats through the data acquisition system composed of MacLab and Macintosh computer. The result of this experiment was as following : COWE increased the changes of rCBF and BP significantly. After pretreatment with propranolol and indomethacin, the changes of rCBF were inhibited significantly, but not by L-NNA and methylene blue. Also, after pretreatment with propranolol, the changes of BP were inhibited significantly, but not by L-NNA, methylene blue and indomethacin. According to above result, it is considered that Catalpa Ovata increased the rCBF and BP, related to adrenergic β-receptor.
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Airway Smooth Muscle에 미치는 자울(紫울)의 효과
나경상 ( Kyung Sang Na ),권의광 ( Eui Kwang Kwon ),소응향 ( Yeung Hyung Soo ),서은미 ( Eun Mi Suh ),한종현 ( Jong Hyun Han ) 대한경락경혈학회 2001 Korean Journal of Acupuncture Vol.18 No.1
Radix Asteris has been used in Korea for many centuries as a treatment for respiratory disease. The effect of Radix Asteris on tracheal smooth muscle is not known. The purpose of the present study is to determine the effect of Radix Asteris on histamine induced tracheal smooth muscle contraction in rats and guinea pigs. Guinea pig(500g, male) and Sprague Dawley rats (250g, male) were killed by CO2 exposure and a segment (8-10mm) of the thoracic trachea from each rat and guinea pig was cut into equal segments and mounted `in pairs` in a tissue bath. Contractile force was measured with force displacement transducers under 0.5g loading tension. The dose of histamine (His) which evoked 50% of maximal response (ED50) was obtained from cumulative dose response curves for histamine (10(-7)~10(-4)M). Contractions evoked by His (ED50) were inhibited significantly by Radix Asteris. In guinea pig tracheal smooth muscle, the mean percent inhibition of histamine induced contraction was 120.5% (p<0.01) after 100㎕/㎖Radix Asteris. In rat tracheal smooth muscle, the mean percent inhibition of histamine induced contraction was 135.4% (p<0.01) after 100㎕/㎖Radix Asteris. Propranolol (10(-7)M) slightly but significantly attenuated the inhibitory effects of Radix Asteris. Following treatment with propranolol, the mean percent inhibition caused by 100㎕/㎖Radix Asteris fell to 44.6% in guinea pig induced by histamine contraction and by 100㎕/㎖Radix Asteris fell to 18.7% (p<0.05) in rat induced by histamine contraction. Indomethacin and methylene blue(10(-7)M) did not significantly alter the inhibitory effect of Radix Asteris. These results indicate that Radix Asteris can relax histamine induced contraction of guinea pig and rat tracheal smooth muscle, and that this inhibition involves sympathetic effects.
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