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rpoB 염기서열 분석을 이용한 응고효소 음성 포도알세균 분자 동정
성원진,김단일,김은경,고대성,노영혜,김재홍,권혁준,Seong, Won-Jin,Kim, Danil,Kim, Eun-Kyung,Ko, Dae-Sung,Ro, Younghye,Kim, Jae-Hong,Kwon, Hyuk-Joon 대한수의학회 2018 大韓獸醫學會誌 Vol.58 No.1
Bovine mastitis (BM) has resulted in enormous economic loss in the dairy industry and coagulase-negative staphylococci (CNS) have caused subclinical BM. Although VITEK 2 GP ID card (VITEK 2) has been used for CNS identification, the probability of identification varies. The rpoB sequence typing (RSTing) method has been used for molecular diagnosis and epidemiology of bacterial infections. In this study, we undertook RSTing of CNS and compared the results with those of VITEK2 and 16S rRNA gene sequencing. As compared VITEK2, the molecular-based methods were more reliable for species identification; moreover, RSTing provided more molecular epidemiological information than that from 16S rRNA gene sequencing.
젖소 유방염에서 16S rRNA 파이로시퀀싱을 이용한 우유 내 마이크로바이옴의 동정과 난백의 항균효과
김단일,김은경,성원진,노영혜,고대성,김남형,김재홍,권혁준,Kim, Danil,Kim, Eun-Kyung,Seong, Won-Jin,Ro, Younghye,Ko, Dae-Sung,Kim, Nam-Hyung,Kim, Jae-Hong,Kwon, Hyuk-Joon 대한수의학회 2017 大韓獸醫學會誌 Vol.57 No.2
Bovine mastitis is an important microbial disease in the dairy industry. We investigated the frequencies of bacterial pathogens in 62 farms and pathogen antibiotic resistance from mastitis samples (n = 748). We tested the antimicrobial activity of chicken and duck egg white and lysozyme purified from chicken egg white. Moreover, we compared the microbiomes of normal and mastitic raw milk obtained by 16S rRNA gene pyrosequencing and culture methods. The results showed that the frequencies of Gram-positive pathogens (Enterococcus faecalis 37% and Staphylococcus aureus 36%) were higher than that of a Gram-negative pathogen (Escherichia coli 15%). Resistance frequencies to ampicillin and norfloxacin were lowest in Staphylococcus aureus (21%), Enterococcus faecalis (23%), and Escherichia coli (33%), and the antimicrobial activity of chicken egg white was higher than those of lysozyme and duck egg white. Pyrosequencing results revealed clear differences between the microbiomes of mastitic and normal raw milk samples and revealed a slightly similar, but clearly different, composition of pathogens compared to that from the culture method. Thus, pyrosequencing may be useful for elucidating changes in microbiomes during mastitis progression and treatment. A chicken egg white and antibiotic combination may help with mastitis treatment; however, further studies are needed.
Chlamydophila psittaci에 감염된 앵무새의 임상병리학적 특징과 원인균의 ompA 유전자 비교분석
김일환,장진욱,이수형,김대용,성원진,권혁준,김재홍,Kim, Il-Hwan,Jang, Jin-Wook,Lee, Su-Hyung,Kim, Dae-Yong,Seong, Won-Jin,Kwon, Hyuk-Joon,Kim, Jae-Hong 대한수의학회 2012 大韓獸醫學會誌 Vol.52 No.3
Avian chlamydiosis is caused by Chlamydophila psittaci and considered as one of an important zoonotic disease throughout the world. Among more than 400 avian species including poultry and pet birds susceptible to the disease, psittacine birds were known to be mostly susceptible hosts. In Korea, no outbreak of the disease and genetic analysis of the agent in poultry and pet birds have been reported. With histopathological findings and genetic identification of a causative agent, avian chlamydiosis was identified in parrots submitted from the same pet bird farm in 2006 and 2009 for the diagnosis. Based on genetic sequences and phylogenetic analysis of ompA gene, the two isolates of Chlamydophila psittaci showed 100% of genetic similarity and belonged to genotype A, suggesting that the same agent might be continuously circulated in the farm. This result indicates that serological survey of the disease in pet bird farms and impact of the disease on significance in public health may be further studied.