Gemcitabine의 세포사멸 기전 연구

설재원,이유진,강동원,강형섭,김남수,김인식,박상열,Seol, Jae-Won,Lee, You-Jin,Kang, Dong-Won,Kang, Hyung-Sub,Kim, Nam-Soo,Kim, In-Shik,Park, Sang-Youel 대한수의학회 2005 大韓獸醫學會誌 Vol.45 No.4

The nucleoside analogue gemcitabine (2', 2-difluorideoxycytide) is potential against a wide variety of solid tumors and considered to be one of the most active drugs in the treatment of non-small cell lung cancer (NSCLC). In this study, we investigated the signals of gemcitabine-induced apoptosis, especially in point of caspase pathway in A549. We exposed A549 cells to gemcitabine for dose/time dependent manner and the results showed that gemcitabine induced apoptotic cell death in a time/dose-dependent manner. We also treated to gemcitabine and Z-VAD-fmk as a pan-caspase inhibitor for 24 hours. Gemcitabine alone induced 35.3% cell death, and co-treatment with gemcitabine and Z-VAD-fmk induced 15.1% apoptotic cell death. Our results demonstrated that Z-VAD-fmk as a pan-caspase did not completely block the gemcitabine-induced apoptosis. Western blotting analysis showed that gemcitabine increased caspase-3, active caspase-8, p21 and p53 protein expressions in A549. Co-treatment with Z-VAD-fmk completely blocked caspase-3 and active caspase-8 protein expressions, but did not change the level of p21 and p53 protein expressions. Our data indicate that gemcitabine induced apoptosis through caspase-dependent and -independent pathways in A549.

Tea Tree Oil의 여드름균에 대한 항균활성

설재원,송근호,이광래,Seol, Jae-Won,Song, Kun-Ho,Lee, Kwang-Rae 강원대학교 산업기술연구소 2011 産業技術硏究 Vol.31 No.2

The purpose of this study is to determine the antimicrobial effect of tea tree oil a against pathogens relate to acne. Tea tree oil extracted from Australian Melaleuca alterifolia has been studied extensively for their antimicrobial properties against different type of bacteria and fungi. Tea tree oil has been reported to have antibacterial, antiinflammatory and antitumor activities, among others. By evaluating the antibacterial effect of Tea Tree Oil of Australia, the MIC value of Tea Tree oil against P. acnes was 0.05% and 0.5% against aerobic bacteria. This study showed that Tea Tree Oil has antibacterial effect against bacteria which induce acne. Tea Tree Oil had better antibacterial effect against P. acnes compared to aerobic bacteria. It is promising to develop a nature-based functional material for makeup product, which can cure and prevent acne.

산삼배양액 급여에 따른 육계의 생산성 및 질병 저항성 효과

설재원,박재홍,채준석,강형섭,류경선,강춘성,박상열,Seol, Jae-Won,Park, Jae-Hong,Chae, Joon-Seok,Kang, Hyung-Sub,Ryu, Kyeong-Seon,Kang, Chun-Seong,Park, Sang-Youel 대한수의학회 2010 大韓獸醫學會誌 Vol.50 No.2

The large amount of tissue culture medium (TCM), which contains some of the active secretory components of Korean wild ginseng (KWG; Panax ginseng) such as saponins, is usually discarded after harvest of KWG. The present study was aimed to investigate the efficacy of oral administration of the TCM-KWG on growth performance and diseases resistance in broiler chickens. A day old broiler chickens randomized in 6 groups (n = 60/groups) were administered orally with 0, 2, 4, 8, 16 and 32 mL/L TCMKWG through drinking water for 5 weeks and examined the change of weight gain, feed intake and blood components. Also, five weeks old broiler chickens (n = 15/groups) were challenged orally with Salmonella (S.) gallinarum and investigated the mortality in broiler chickens. An average weight gain and feed intake significantly didn't change in TCM-KWG administration groups as compared to control group. The concentration of calcium (Ca), phosphate (Pi) and potassium (K) in serum were increase by TCM-KWG administration in broiler chickens. We also found that oral administration of TCM-KWG through drinking water significantly reduced the mortality in broiler chickens experimentally infected with virulent S. gallinarum. The results of this study indicated that TCM-KWG administration may elevate the resistance on disease and improved the skeleton formation and body homeostasis of chickens, and TCM-KWG can be used as a cost-effective and environmentally alternative additives to control of the disease and growth.

산삼 배양액을 급여한 육계에서 근육의 프로테옴 분석

설재원,황인호,채준석,강형섭,류경선,강춘성,박상열,Seol, Jae-Won,Hwang, In Ho,Chae, Joon-Seok,Kang, Hyung-Sub,Ryu, Kyeong-Seon,Kang, Chun-Seong,Park, Sang-Youel 대한수의학회 2005 大韓獸醫學會誌 Vol.45 No.2

Proteomics is a useful approach to know protein expression, post-translational modification and protein function. We investigated the protein expression pattern and identity in chickens fed with the tissue culture medium waste after harvest of Korean wild ginseng (TCM-KWG) (Panax ginseng). Two groups (n=60/group) of day old broiler chickens were administered with 0 (control) and 0.8% (treatment) TCM-KWG through drinking water. After 5 weeks, we examined the protein expression pattern of fibularis longus and superficial pectoral muscle by Two-dimensional electrophoresis analysis. Interestingly, TCM-KWG treatment significantly increased five spot's density, and markedly reduced five spot's density in the muscles. We identified 10 proteins (desmin, myosin light chain 1, heat shock 25 kDa protein, collapsin response mediator protein-2A, alpha enolase, vimentin, actin alpha 1, my023 protein, pyruvate kinase and troponin T) by the matrix-assisted laser desorption ionization time of flight (MALDI-TOF).

Proteasome 억제에 의한 P53의 발현과 미토콘드리아 막 전압의 소실로 TRAIL에 저항하는 폐암세포의 사멸 강화

설재원,박상열,Seol, Jae-Won,Park, Sang-Youel 대한수의학회 2009 大韓獸醫學會誌 Vol.49 No.1

The ubiquitin-proteasome mediated protein degradation pathway plays an important role in regulating both cell proliferation and cell death. Proteasome inhibitors are well known to induce apoptosis in various human cancer cell lines. We investigated the effect of combined treatment with proteasome inhibitor and TRAIL, and a possible mechanism of the enhancing apoptosis by the both treatment, on TRAIL-resistant non-small cell lung cancer. A549 cells were exposed to the N-Acetyl-Leu-Leu-Norleu-al (ALLN) as a proteasome inhibitor and then treated with recombinant TRAIL protein. In A549 cells under proteasome inhibition conditions by pretreatment with ALLN, TRAIL treatment significantly decreased cell viability compared to that ALLN and TRAIL alone treatment. Also, the both treatment induced cell damage through DNA fragmentation and p53 expression. In addition, the combined treatment of both markedly increased caspase-8 activation, especially the exposure for 2 h, and Bax expression and induced the dissipation of mitochondrial transmembrane potential in A549 cells. Taken together, these findings showed that proteasome inhibition by ALLN enhanced TRAIL-induced apoptosis via DNA degradation by activated P53 and mitochondrial transmembrane potential loss by caspase-8 activation and bax expression. Therefore, our results suggest that proteasome inhibitor may be used a very effectively chemotherapeutic agent for the tumor treatment, especially TRAIL-resistant tumor cell.

산삼 배양액 급여가 육계의 육질에 미치는 영향 연구

설재원(Jae-Won Seol),박재홍(Jae-Hong Park),채준석(Joon-Seok Chae),강형섭(Hyung-Sub Kang),류경선(Kyeong-Seon Ryu),강춘성(Chun-Seong Kang),박상열(Sang-Youel Park) 韓國家禽學會 2010 韓國家禽學會誌 Vol.37 No.2

본 연구에서는 산삼의 조직 배양 후 폐기되는 산삼 배양액을 육계에 음수를 통하여 다양한 농도로 급여한 후 계육의 일반 성분, 물리화학적인 성상 및 이화학적 성상 변화를 분석하여 산삼 배양액의 효능과 첨가제로서의 가능성을 규명하였다. 산삼 배양액을 급여한 육계 가슴육의 수분 함량, 조단백질과 조지방의 함량에 유의적인 차이는 보이지 않았지만 조회분의 함량은 산삼 배양액 32 mL/L 급여군이 다른 처리군에 비하여 유의적으로 증가하는 것을 볼 수 있었다. 또한, 산삼 배양액 급여군에서 육즙 손실, 조리 감량, 전단력과 저장성은 감소되는 경향을 보이나 유의적인 차이는 보이지 않았지만 산삼 배양액 급여군의 가슴육 pH는 유의성 있게 감소하였다. 이러한 결과는 산삼 배양액의 급여에 의해 육제품에서 미생물의 번식을 억제하고 보존이나 내구성을 강화시키거나, 육계의 골격 형성과 육질 내 삼투압이나 항상성 유지에 도움을 줄 것으로 사료된다. TCM-KWG (tissue culture medium waste after harvest of Korean wild ginseng) (panax ginseng) is left over of tissue culture medium used to grow Korean wild ginseng (KWG). The present study was conducted to investigate the effect of TCM-KWG on meat quality and the possible of application as additives in broiler chickens. A day old broiler chickens randomized in 6 groups (n=60/groups) were administered orally with 2, 4, 8, 16, 32 mL/L TCM-KWG through drinking water with one untreated control group. After administration for five weeks, we analyzed chemical composition and meat quality. Crude ash increased approximately 20% in TCM-KWG treatment groups as compared to control group whereas the concentration of moisture, crude protein and crude fat did not show any significant difference. Crude ash is essential to enhance skeleton formation and physiological function. TCM-KWG treatment gradually decreased the pH value of breast meat whereas it did not change the purge loss and cooking loss. The pH value of meat is important for preservation of meat for longer periods and high durability strength. These results suggest that TCM-KWG treatment may improve the quality of meat and can be apply as food additives in chickens.

Tea Tree Oil의 여드름균에 대한 항균활성

설재원(Seol, Jae-Won),송근호(Song, Kun-Ho),이광래(Lee, Kwang-Rae) 강원대학교 산업기술연구소 2011 産業技術硏究 Vol.31 No.1

The purpose of this study is to determine the antimicrobial effect of tea tree oil a against pathogens relate to acne. Tea tree oil extracted from Australian Melaleuca alterifolia has been studied extensively for their antimicrobial properties against different type of bacteria and fungi. Tea tree oil has been reported to have antibacterial, antiinflammatory and antitumor activities, among others. By evaluating the antibacterial effect of Tea Tree Oil of Australia, the MIC value of Tea Tree oil against P. acnes was 0.05% and 0.5% against aerobic bacteria. This study showed that Tea Tree Oil has antibacterial effect against bacteria which induce acne. Tea Tree Oil had better antibacterial effect against P. acnes compared to aerobic bacteria. It is promising to develop a nature-based functional material for makeup product, which can cure and prevent acne.

개 연골세포의 손상에 의한 Tartrate Resistant Acid Phosphatase 활성의 변화 측정

설재원 ( Jae Won Seol ),이해범 ( Hae Beom Lee ),김남수 ( Nam Soo Kim ),김인식 ( In Shik Kim ),강형섭 ( Hyung Sub Kang ),이영훈 ( Young Hoon Lee ),강동원 ( Dong Won Kang ),박상열 ( Sang Youel Park ) 한국임상수의학회 2006 한국임상수의학회지 Vol.23 No.2

TRAIL 유도 세포사멸에 있어서 IFN-γ의한 증가 기전 연구: IRF-1과의 관련성

박상열,설재원,이유진,강석진,김인식,강형섭,채준석,조종후,Park, Sang-Youel,Seol, Jae-Won,Lee, You-Jin,Kang, Seog-Jin,Kim, In-shik,Kang, Hyung-sub,Chae, Joon-seok,Cho, Jong-Hoo 대한수의학회 2004 大韓獸醫學會誌 Vol.44 No.2

Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a member of the TNF family and potent inducer of apoptosis. TRAIL has been shown to effectively limit tumor growth in vivo without detectable cytotoxic side effects. Interferon (IFN)-${\gamma}$ often modulates the anti-cancer activities of TNF family members including TRAIL. We previously reported that IFN-${\gamma}$ enhanced TRAIL-induced Apoptosis in HeLa cells without the unknown mechanism. In this study, we investigated whether IRF-1 involves in IFN-${\gamma}$-enhanced TRAIL-induced apoptosis. We exposed HeLa cells to IFN-${\gamma}$ for 12 hours and then treated with recombinant TRAIL protein. No apoptosis was induced in cells pretreated with IFN-${\gamma}$, and TRAIL only induced 30% apoptosis after 3 hours treatment. In HeLa cells pretreated with IFN-${\gamma}$, TRAIL induced cell death to more than 75% at 3 hours, showed that IFN-${\gamma}$-pretreatment enhanced HeLa cell death to TRAIL-induced apoptosis. To investigate the functional role of IRF-1 in IFN-${\gamma}$-enhanced TRAIL-induced apoptosis, IRF-1 was overexpressed by using an adenoviral vector AdIRF-1. IRF-1 overexpression increased apoptotic cell death and significantly enhanced apoptotic cell death induced by TRAIL when infected cells were treated with TRAIL. Our findings show that IFN-${\gamma}$ enhances TRAIL-induced apoptosis by IRF-1 in HeLa cells.

생물다양성을 보존하기 위한 토대로서 생태다양성 분석 및 복원 전략

임봉순,김동욱,김아름,설재원,이창석,Lim, Bong Soon,Kim, Dong Uk,Kim, A Reum,Seol, Jae Won,Lee, Chang Seok 한국하천호수학회 2020 생태와 환경 Vol.53 No.4

This study aims to establish the national strategy for biodiversity conservation by analyzing the current status of ecodiversity as the foundation of biodiversity conservation. Furthermore, this study has another purpose of preparing the measures for conservation and restoration of biodiversity. Ecodiversity was discussed as the basis for conserving biodiversity. Five climate zones and 14 climatic regions, eight plant geographic regions, three massifs and major geologic series, horizontal and vertical topographic conditions, 16 ecoregions, major ecosystems including forest, river and streams, wetlands, coast and marine, agriculture, and urban esosystems, and land use types were discussed as the element of the ecodiversity. In terms of biodiversity conservation, the actual conditions of each ecological unit were reviewed and measures were proposed to reduce biodiversity loss. Destruction and fragmentation of habitat, poor ecosystem management due to socioeconomic changes, the effects of exotic species and chemicals, and climate change were discussed as the major factors causing biodiversity loss. Systematic monitoring based on scientific principles and ecological restoration based on those monitoring results were recommended as measures for biodiversity conservation.