조각자(주엽) 나무의 생리활성물질 Gleditschia이 비육돈육의 이화학적 성상에 미치는 영향

서종립,허정호,정명호,조명희,이국천,김국헌,하대식,류재두,김충희,김곤섭,김의경,김종수,Seo, Jong-Lip,Heo, Jung-Ho,Jung, Myung-Ho,Cho, Myung-Heui,Lee, Kuk-Cheon,Kim, Kuk-Hun,Hah, Dae-Shik,Ryu, Jae-Doo,Kim, Chung-Hui,Kim, Gon-Sup,Kim, Eui-Gy 대한수의학회 2005 大韓獸醫學會誌 Vol.45 No.4

The effects of dietary Gleditschia on fatty acid composition, lipid oxidation, and pork quality were investigated. Pigs (n=40) were fed a diet containing 0, 0.1, 0.2, 0.4, and 1% Gleditschia for 14 weeks and slaughtered at 110 kg average. The longissimus throracis et lumborum muscle was collected at 24 hr postmortem. Pork loin chops (3 cm thick) were packaged aerobically and stored at $4^{\circ}C$ for 7 days. Samples were analyzed for fatty acid composition, ultimate pH, thiobarbituric acid-reactive substance (TBARS), color ($L^*$, $a^*$, $b^*$), drip loss and water-holding capacity. There was no significant difference of moisture and crude fat percent in between treatment and control group, and the color stability of pork loin better in all treat groups was more improved during cold storage. The change of pH was significantly increased (p<0.05) in 0.2, 0.4, 1% treatment groups compare to the control group. Water-holding capacity of pig loins was significantly higher (p<0.05) in all treat groups than in control group; 0.2% treat group was the highest in the water holding capacity followed by 0.4, 1, and 0.1% treat groups. Less drip loss of pig loin was observed with samples from Gleditschia-fed pigs except 1% treat group. Unsaturated fatty acid were tend to be decreased and saturated fatty acid were tend to be increased in Gleditschia-treated group. Thiobarbituric acid-reactive substance value of control was significantly higher than that of the Gleditschia-fed group (p<0.05).

호흡기 증상을 나타낸 소에서 Pasteurella spp의 분리 및 약제감수성

김종수,허정호,정명호,조명희,김남철,이국천,서종립,손성기 한국임상수의학회 2001 한국임상수의학회지 Vol.18 No.2

The present study was conducted to investigate isolation and antimicrobial drug susceptibility of Pasteurella spp from pneumonic calves and cows (pneumonic lungs) in Gyeongnam south area from January to November 2000. Pasteurella multocida and P haemolytica were isolated 136 (53.8%) and 22(8.7%), respectively, from 158 of 253 pneumonic calves of 1 to 12 months of age and from pneumonic lungs and simultaneous isolation rate of P mutocida and P haemolytica was 4 (1.6%). Seasonal isolation rate of Pasteurella spp in calves and pneumonic lungs varied from 3 to 65.2%, and it was higher in Fall. The majority of biochemical and cultural properties of P multocida and P haemolytica isolated from calves and pneumonic lung were identical to those of the reference strains employed. Pneumonic lesions of the examined lungs were histologically identified as purulent bronchopneumonia and fibrinous pneumonia. All isolates were resistant to penicillin, amikacin, lincomicin, colistin, cloxacilling, sulfonamide, kanamycin, sulfamethoxasoletrimetoprim, and gentamicin(>80%), but some of them were susceptible to amoxicillin, norfloxacin, cephalexin, cefazolin, orbifloxacin, enrofloxacin, and ciprofloxacin(>60%).

屠鷄場 出荷닭에 대한 구포자충 感染 實態 調査

허정호,정명호,김국헌,조명희,이국천,서종립,김충희,하대식,류재두,김곤섭,김의경,김종수 한국임상수의학회 2004 한국임상수의학회지 Vol.21 No.2

The present study was conducted to investigate the prevalence of Eimeria infection in 1,300 slaughtered chickens from 130 farms in Gyeongnam, Jeonnam, Jeonbuk, Gyeongbuk and Chungnam during the period from October 2002 to August 2003. The results obtained are summarized as follows : 1. The prevalence of Eimeria infection in 130 chicken farms was 76.9% and Eimeria infection rates of Gyeongnam, Jeonbuk, Gyeongbuk, Jeonnam and Chungnam were 80%, 75%, 70%, 42.9%, respectively. That of Tongyoung was the highest infection(94.4%) than others districts in the Gyeongnam. 2. The prevalence of oocysts infection level in 1,300 slaughtered chickens from 5 districts was 33.4% and that of Jeonbuk was the highest rate(42%) and Gyeongbuk has the lowest rate(20%). Prevalence of OPG level of slaughtered chicken in Tongyoung was the highest rate(42.8%) in Gyeongnam. 3. Mixed infection rate of over types 2 was 79% and triple infection was shown the highest infection(33%) among the infection types. 4. Kinds of E. spp. were E. acerulina, E. mivati, E. necatrix, E. maxima, and E. tenella. E. tenella has the highest infection rate(78%) and E. necatrix has the lowest infection rate(36%) among the Eimeria spp. 5. The monthly prevalence of Eimeria infection was shown higher in July(94.4%) compared with other months and Jun was shown lower(57.9%). But, level of OPG was shown higher in summer season(July, August; over 40%) than that of winter season(January, February; 23.1%, 16%).

경남지역 야생들쥐에서 Orientia tsutsugamushi에 대한 항체 조사 및 PCR에 의한 검색

하대식,김영훈,박정웅,박재갑,김충희,류재두,정명호,허정호,서종립,조명희,이국천,김곤섭,김의경,김종수,Hah, Dae-Sik,Kim, Young-Hoon,Park, Jung-Ung,Park, Jae-Kap,Kim, Chung-Hui,Ryu, Jae-Doo,Jong, Myung-Ho,Heo, Jung-Ho,Shu, Jong-Lip,Cho, Myung-Heui 대한수의학회 2004 大韓獸醫學會誌 Vol.44 No.2

As a part of epidemiologic investigation of tsutsugamushi disease, the wild rodents which were captured in Gyeongnam area were diagnosed with indirect immunofluorescent antibody assay (IFA) and Polymerase Chain Reaction (PCR) to find if they have an antibody against Orientia tsutsugamushi. The conclusion was drawn as followings. (1) The captured 58 wild rodents showed that the subspecies distribution of Apodemus agrarius was 86.2%, Microtus fortis was 8.6% and Crocidura lasiura was 5.2%. (2) The antibody positive rate of O. tsutsugamushi Gilliam, Karp, Karto and Boryong by IFA method was 32.0% in Apodemus agrarius among 50 wild rodents and 40.0% in Microtus fortis among 5 wild rodents, respectively. It was negative in the case of all the 3 Crocidura lasiura. (3) The antibody titers on Apodemus agrarius, Microtus fortis and Crocidura lasiura against Gilliam, Karp, Karto and Boryong were measured between 1:20 and 1:640. The antibody titer against each antigen was in the order Boryong>Gilliam>Karp. (4) O. tsutsugamushi was detected from the blood, spleen and kidney from the artificially infected mice by IFA and PCR. IFA showed the positive response between 3 and 18 days after inoculation. On the other hand, positive response was found from all the samples by PCR. (5) From PCR of the genomic DNA extracted from the blood, spleen and kidney samples of the captured wild rodents, Boryong-specific amplification product with size of 210 bp, which is particular in Boryong, was detected from spleen and kidney samples, but not detected in the blood. (6) Boryong-specific amplification product was detected from spleen and kidney samples which were obtained at 3, 6, 12, 18 and 24 days after the infection with Boryong. But, it wasn't detected from the uninfected samples. (7) From PCR of spleen and kidney samples of the captured wild rodents, it was found that positive rate of O. tsutsugamushi in Apodemus agrarius and Microtus fortis were 25.0% (4/16) and 20.0% (1/5), respectively. From the above results, it can be concluded that Apodemus agrarius resided in Gyeongnam area carried O. tsutsugamushi and PCR method might be a simple, precise, rapid and useful diagnostic tool than IFA for the diagnosis of O. tsutsugamushi.

항구포자충제제의 사용 여부에 따른 구포자충 감염실태 조사

허정호 ( Jung Ho Heo ),정명호 ( Myung Ho Jung ),김국헌 ( Kuk Hun Kim ),조명희 ( Myung Heui Cho ),이국천 ( Kuk Cheon Lee ),서종립 ( Jong Lip Seo ),김충희 ( Chung Hui Kim ),하대식 ( Dae Sik Hah ),류재두 ( Jae Doo Ryu ),김곤섭 ( Gon 한국가축위생학회 2005 韓國家畜衛生學會誌 Vol.28 No.3

The present study was conducted to investigate the prevalence of Eimeria infection according to anti-coccidiosis drugs treatment in 1,300 slaughtered chickens from 130 farms in Gyeongnam, Jeonnam, Jeonbuk, Gyeongbuk and Chungnam during the period from October 2002 to August 2003. The prevalence of Eimeria infection by drug administration for treatment and prevention or non-administration was shown 71.4%, 82.6% and 72.3%, respectively, and so infection rate of chicken farms was similar independent on drug administration or not. The prevalence of Oocysts infection level of chicken farms by administrated drug for treatment was shown lower (25.3%) than for prevention (35.4%) and non-treatment (36.2%). The prevalence of Oocysts infection level by drug administration under 2 days for treatment was shown higher infection rate (32.2%) than over 3 days (20%) and also under 2 days for prevention has similar tendency of that of treatment and so, it was conclusion that administration of drug over 3 days has the effect for treatment and prevention of Eimeria infection. The prevalence of Oocysts infection level by age for prevention has various level from 48.8% to 22.9% but on 22~25 days was shown the most higher ratio (48.8%) and on 19∼21 days was shown the lowest rate (22.9%) among the all age groups.