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Neurospora crassa 의 NAD glycohydrolase messenger RNA 정제 및 in vitro translation
김우현,서정선,김형로 ( Uh Hyun Kim,Jeong Sun Seo,Hyung Rho Kim ) 생화학분자생물학회 1986 BMB Reports Vol.19 No.1
The in vitro translation products were obtained by translating the isolated Neurospora messenger RNA from Neurospora crassa cultured in Fries minimal medium and zinc and nitrogen deficient medium and the NAD glycohydrolase (NADase) (EC3.2.2.5) was identified in the translation products by the immunoprecipitation methods. The yields of total RNA and messenger RNA from Neurospora crassa grown in zinc and nitrogen deficient media were lower than those from control media but the function of the synthesis of proteins was not affected. The synthesis of NADase-specific mRNA seemed not to be directly proportional to the increase of the specific activity of the enzyme. The increase of NADase in Neurospora crassa grown in zinc and nitrogen deficient seemed not to be ascribed to the regulation in the level of transcription. The molecular weight and the isoelectric point of NADase in the translational products was around 6,000 dalton and pI 9.0, respectively which were different from the other data obtained from purified Neurospora NADase, suggesting that this translation product was not processed post-translational modification.
김우현,서정선,김형로,Kim, Uh-Hyun,Seo, Jeong-Sun,Kim, Hyung-Rho 생화학분자생물학회 1986 한국생화학회지 Vol.19 No.1
Fries minimal 배지와 zinc-nitrogen 결핍 배지에서 각각 배양한 Neurospora crassa로부터 messenger RNA을 얻었다. cell-free reticulocyte system을 이용하여 Neurospora mRNA로부터 in vitro translation 산물을 얻고 면역침전법을 이용하여 이 단백합성 산물내에서 NAD glycohydrolase (EC 3.2.2.5)를 확인하였다. zinc-nitrogen 결핍배지에서의 Neurospora RNA와 mRNA양은 정상배지에서의 양에 비하여 감소하였으나 mRNA의 단백합성능력에는 이상을 보이지 않았다. zinc-nitrogen 결핍배지에서 자란 Neurospora에서 NAD glycohydrolase 특이적 mRNA의 합성을 효소의 비활성도의 증가에 비례하여 증가되지 않은 것으로 보아 NAD glycohydrolase의 증가는 transcription level에서의 조절이 아닌 것으로 사료된다. SDS-polyacrylamide gel 전기영동과 isoelectrofocusing에 의한 단백 합성 산물내의 NAD glycohydrolase 분자량과 pI는 각각 6,000 dalton과 9.0으로서 cell-free reticulocyte system에서의 in vitro translation에서는 post-translational modification이 일어나지 않았음을 시사해 주었다. The in vitro translation products were obtained by translating the isolated Neurospora messenger RNA from Neurospora crassa cultured in Fries minimal medium and zinc and nitrogen deficient medium and the NAD glycohydrolase (NADase) (EC3.2.2.5) was identified in the translation products by the immunoprecipitation methods. The yields of total RNA and messenger RNA from Neurospora crassa grown in zinc and nitrogen deficient media were lower than those from control media but the function of the synthesis of proteins was not affected. The synthesis of NADase-specific mRNA seemed not to be directly proportional to the increase of the specific activity of the enzyme. The increase of NADase in Neurospora crassa grown in zinc and nitrogen deficient seemed not to be ascribed to the regulation in the level of transcription. The molecular weight and the isoelectric point of NADase in the translational products was around 6,000 dalton and pI 9.0, respectively which were different from the other data obtained from purified Neurospora NADase, suggesting that this translation product was not processed post-translational modification.
Banded Smith - Waterman 알고리즘을 이용하여 정규화된 부분배치를 찾는 새로운 알고리즘
김상태(Sangtae Kim),심정섭(Jeong Seop Sim),박희진(Heejin Park),박근수(Kunsoo Park),박현석(Hyunseok Park),서정선(Jeong-Sun Seo) 한국정보과학회 2001 한국정보과학회 학술발표논문집 Vol.28 No.2Ⅰ
두 문자열의 부분배치(local alignment)를 찾는 대표적인 알고리즘인 Smith-Waterman 알고리즘(SW 알고리즘)은 정규화된 최적 부분배치를 찾지 못하는 단점이 있다. 최근에 fractional programming 기법을 이용하여 여러 변의 SW 알고리즘을 수행함으로써 정규화된 최적부분배치를 찾는 알고리즘이 제시되었지만 이는 매우 많은 시간이 걸린다. 본 논문에서는 fractional programming 기법을 이용하여 정규화된 최적부분배치를 찾는 알고리즘에, 완전매치(Exact Match)를 이용한 휴리스틱 기법인 Banded SW 알고리즘 적용하여, 낮은 오차를 가지면서 실용적으로는 매우 빠른 정규화된 최적부분배치를 찾는 알고리즘을 제시하고 이 알고리즘과 기존의 알고리즘을 직접 구현하여 실험한 결과를 비교 분석한다.
간염 (肝炎) B 바이러스 DNA 혈중농도 (血中濃度)와 HBeAg / Anti - HBe 와의 상관 관계
송인성(In Sung Song),김정룡(Chung Yong Kim),이효석(Hyo Suk Lee),최상운(Sang Woon Choi),서정선(Jeong Sun Seo) 대한소화기학회 1989 대한소화기학회지 Vol.21 No.4
N/A To evaluate the relationship between serum HBV DNA positivity and HBeAg/anti-Hbe status, and also the feasibility of HBeAg titer as an indirect parameter of serum HBV DNA level, 275 HBsAg- positive patients with acute and chronic liver disease were included in this study. The positive rate for serum HBV DNA in case of HBeAg positive/anti-HBe negative, HBeAg negative/anti-HBe negative, and HBeAg negative/anti-HBe positive was 92.5%, 63.3%, and 40.4%, respectively. Although the positive rate of HBV DNA in case of HBeAg positive/anti-HBe negativie was singificantly higher (p < 0.05) than the others, it was as high as about 50% (82/169) even in HBeAg negative cases regardless of anti-HBe positivity. The close correlation between log2 HBeAg titer and HBV DNA level in the serum was not demonstrable. We could, therfore, conclude that either detection of IIBeAg or measurement of HBeAg titer could not replace the determination of serum HBV DNA for the evaluation of infectivity of the HBsAg positive sera.