쥐하지부 동종이식에서 FK-506과 Rapamycin을 사용하여 이식부 생존기간에 대한 효과의 비교관찰

서인석,Suh, In-Suck,Lineaweaver, William C. 대한미세수술학회 1995 Archives of reconstructive microsurgery Vol.4 No.1

Free vascularized composite tissue transfer is more frequently underwent for reconstruction of complicated tissue defects with the recent advance of microsurgery. But postoperative result was not satifactory because of donor site morbidity, flap bulkiness and cosmetic problem. So would no longer be a problem if we can obtain the exact donor tissue required for the recipient site as allotransplantation and designing the flap. Allotransplantation has been resolved with the recent development of immunosuppressive agents, while reconstruction has made great progress with the refinement of microsurgical techniques in the last 20 years. The final sucess or failure of the operative procedure in transplantation is so utterly dependent no the availability of strategies that can control the immune system effectively, selectively, safely to allow allotransplantation of a nonvital body part. 1 used 2 strains of rats, BUF and LEW, for the limb allotransplantation as a composite tissue transfer. The primary goal of this program is to improve results in clinical transplantation by accelerating the transformation of new immunological knowledge into useful medicine. Two of the most promising new immunosuppressive compounds are FK-t06(FK) and rapamycin(RPM). Both drugs are antibiotic macrolide fungal fermentation products that presumably suppress the immune system in ways similar to cyclosporin(CyA). This study shows that two new immunosuppressive drugs compare the immunosuppressive activity and effectiveness of FK-506 and RPM for prevention of the limb allograft rejection in the rat. Additional experiments investigate the dose, route of administration and histologic findings. These data demonstrates that rapamycin is far more potent and effective than FK-506 when both compounds are administered by the intraperitoneal route, as well as prolonged graft survival significantly in a dose-route dependent manner. These results lead to the view that vascularized allograft composite tissue transfer can become a reality with the expectation of possible future application in reconstructive surgery of humans.

Electrodeposition of some Alpha-Emitting Nuclides and its Isotope Determination by Alpha Spectrometry

정기석,서인석,Key-Suck Jung,In-Suck Suh Korean Chemical Society 1983 대한화학회지 Vol.27 No.4

몇가지의 알파입자를 방출하는 핵종, 즉 악티늄족 원소들, $^{207}Bi$ and $^{210}Po$을 전해석출하는 장치를 만들었다. 스텐레스 원판으로된 환원전극에 이 동위원소들을 석출했으며(석출부분 직경=18mm), 산화전극으로는 백금선을 썼다. 전해질로 염화암모늄을 쓰고, 초기 pH=4, 염소이온농도=0.6M이하, 용액부피=15ml로 하여 1.5암페어(전류밀도=0.59A/$cm^2$)의 전류를 100분간 흘려주어 98.3%의 석출 회수율과 ${\pm}$0.7%의 재현도를 얻었다. 석출된 시료의 알파스펙트럼을 측정한 결과 에너지 분리도로서 $^{210}Po=18.3keV, ^{234}U=21.8keV$ 및 $^{239}Pu=36.0keV$인 반치전폭(full width at half maximum)을 얻었다. 국산 천연우라늄(충북·괴산) 시료를 전해석출하여 그의 알파스펙트럼을 구한 결과 $^{238}U\;:\;^{234}U\;=\;:\;6.1{\times}10^{-5}$을 얻었으며 $1.8{\sim}10^{13} neutrons/cm^2{\cdot}sec$인 중성자속으로 144일 동안 쪼여준 238U 시료를 전해석출하여 그의 알파스펙트럼을 구한 결과 $^{238}U\;:\;^{239}Pu\;:\;241Am\;=\;100\;:\;0.0263\;:\;5.20{\times}10^{-5}$을 얻었다. 조사시료중의 $^{238}U$에 대한 본실험의 정량결과는 고체형광측정법 및 질량 스펙트럼법에 의한 결과들과 상대오차 1.6% 이내에서 일치하였으며, $^{239}Pu$의 경우는 음이온교환분리-알파스펙트럼 측정 및 삼불화테노일아세톤(thenoyltrifluoroacetone)을 쓴 용매추출-알파스펙트럼 측정에 의한 정량결과들과 상대오차 ${\pm}$4.0%이내에서 일치하였다. An apparatus was made for the electrodeposition of alpha emitting actinide nuclides, $^{207}Bi$ and $^{210}Po$. The electrodeposition was made on a polished stainless steel plate cathode. The anode was made of platinum wire and to stir the solution. With the ammonium chloride as electrolyte initial pH = 4, chloride concentration = 0.6M and solution volume = 15ml, a current of 1.5 ampere(current density = 0.59A/$cm^2$) was flowed for 100 minutes for the quantitative recovery of electrodeposition and on average recovery of 98.3% was obtained within ${\pm}$0.7% uncertainty. Alpha spectrometry of the electrodeposited sample showed alpha peaks from $^{210}Po, ^{234}U$ and $^{239}Pu$ having energy resolution (FWHM) of 18.3, 21.8 and 36.0 keV respectively. The electrodeposition and alpha spectrometry for a natural uranium sample of domestic origin gave $^{238}U : ^{234}U = 1 : 6.1{\times}10^{-5}$ and for a neutron-irradiated uranium sample did $^{238}U : ^{239}Pu : ^{241}Am = 100 : 0.0263 : 5.20{times}10^{-5}$. The result of $^{238}U$ determination in the irradiated sample by electrodeposition-alpha spectrometry was in accord within ${\pm}1.6%$ of relative error with the results of solid fluorimetry and mass spectrometry. For $^{239}Pu$ the result of electrodeposition-alpha spectrometry was in accord within ${\pm}$4.0% of relative error with the results of anion exchange separation and the thenoyltrifluoroacetone(TTA) extraction both followed by alpha spectrometries.

성형외과 영역에서 이물질 주사에 대한 고해상초음파 검사의 유용성

고응열,성하민,조건,박영규,탁경석,서인석,양익,Ko, Eung-Yeol,Sung, Ha-Min,Cho, Geon,Park, Young-Kyu,Tak, Kyoung-Seok,Suh, In-Suck,Yang, Ik 대한성형외과학회 2010 Archives of Plastic Surgery Vol.37 No.4

Purpose: The purpose of this study is to demonstrate the usefulness of the high resolutional ultrasonographic features in patients with foreign body. Methods: From September 2007 to August 2009, we retrospectively reviewed high resolutional ultrasonogram using 5~12 MHz linear transducer of 13 patients presenting with inflammation after foreign body injection. They were referred for complications after foreign body injection. Injected foreign bodies were 4 silicone, 4 paraffin, 2 artecoll, and 3 unknown. We treated them with foreign body removal (7), foreign body removal and corrective plastic surgery (4), and conservative treatment with antibiotics and steroid injection (2). Results: High resolutional ultrasonography well demonstrated the existence of foreign body and it's overall size, location within the tissue layer, and vascularity. Comparison between preoperative and postoperative ultrasonographic findings was useful not only to evaluate the prognosis but also to plan the treatment. These ultrasonographic findings aided in precise assessment of the contour and location of the foreign body and led to an accurate surgery. We were able to acquire various information in order to set a detailed plan for the operation which in turn, led to a precise, successful surgery. After the treatment, complication did not occur in 12 patients, except 1 patient. But this patient was also treated after reoperation. Postoperative high resolutional ultrasonography shows almost foreign body removed and inflammation disappeared. Conclusion: Considering the usefulness of highresolution ultrasonography in foreign body injection, highresolution ultrasonography would be necessary for both the patient and the doctor. Preoperative and postoperative high resolutional ultrasonography is highly accurate, safe, inexpensive and easy. It can be a useful modality in foreign body after plastic surgery.

Electrodeposition of some Alpha-Emitting Nuclides and its Isotope Determination by Alpha Spectrometry

정기석(Key Suck Jung),서인석(In Suck Suh) 대한화학회 1983 대한화학회지 Vol.27 No.4

요배부에 발생한 광범위 연부조직결손부의 조직확장술

정영기(Yung Khee Chung),서인석(In Suck Suh),주의탁(Eui Tak Chu) 대한정형외과학회 1992 대한정형외과학회지 Vol.27 No.1

수부의 연부조직 결손 재건을 위한 유리 피판의 선택

김택규,김한수,최상묵,정찬민,서인석,Kim, Taek-Kyu,Kim, Han-Su,Choi, Sang-Mook,Chung, Chan-Min,Suh, In-Suck 대한미세수술학회 1997 Archives of reconstructive microsurgery Vol.6 No.1

Traumatic injury to the hand often leads to soft tissue defects with exposed tendons, bones, or joints. Though many new flap have been introduced, the choice of flap that would be best for the patient depends on such factors as the site, size, and degree of wounds. Additionally the selected surgical method should be yielded cosmetic and functional superiority by the one-staged reconstruction. In our experience, small to medium sized soft tissue defect with bone and tendon exposure of hand can be resurfaced with an arterialized venous free flap from the volar aspect of distal forearm. Wide and deep defects of the hand can be covered with a sensory cutaneous free flap such as the medial plantar free flap, dorsalis pedis free flap, and radial forearm free flap. Specialized flap such as wrap-around flap, toe-to-finger transfer, onychocutaneous free flap can be used for the recontruction of defect on the thumb and finger. Based on the above considerations and our clinical experience of 60 free flap cases of the hand, the various methods for the proper repair of soft tissue defects of the hand are described. And we obtained satisfactory functional and cosmetic results with 95% success rate of free flap.

조직확장법,술중 조직확장법 및 술전 봉합법의 임상적 응용에 관한 비교 연구

오석준,하지운,김응춘,서인석,조세흠 大韓成形外科學會 1993 Archives of Plastic Surgery Vol.20 No.3

The purpose of soft tissue defect reconstruction is minimal scar with aesthetic and functional good results. Reconstruction of head and neck, upper extremity, chest, and lower extremity defects has been reported with standard available tissue expansions. Various surgical methods have been used in an attempt to achieve a normallooking skin color, texture, sensation, thickness and same skin adenexa. Therefore, in accordance with defect size and location, the operative method was considered so we select adequate method. Authors achieved good results from 48 cases of tissue expansion, 18 cases of ISLE (intraoperative sustained limited expansion), and 35 cases of presuturing technique. 1)The most common cause of reconstruction for soft tissue defect was post grafted scar 51cases(51%), next was tattoo 20 cases(19.6%), traumatic soft tissue defect 16cases(15.7% ) and nevus 8 cases(7.8%)in order. 2)Males(55.9%)were affected more often than females(44.1%)in the ratio 1, 3 : 1 and the most frequently affected group was the age of 20 to 30 years as 36% . 3)The distribution of incidence and location were as follow : face(39.2%), upper extremity(25.5%), scalp(17.6%), lower extremity(9.8%) and trunk(7.8%). 4)The cause and distribution were scalp in 20 cases(39.2%), face in 40 cases(29.4%) in the post grafted scar, upper extremity 18 cases(90.0%) in the tattoo, upper extremity 4 cases(50.0%), face 2 cases(25.0%) in the nevus and even distribution in the traumatic soft tissue defects. 5)The presuturing technique was best recommended method for soft tissue defect in 4cm diameter lesion on extremity, but ISLE method was used in the open traumatic soft tissue defect. Over 9cm wide scar was resurfaced by expanded skin using tissue expander.

고령환자 성형재건술에 대한 분석

김택규,서인석 大韓成形外科學會 1997 Archives of Plastic Surgery Vol.24 No.2

In modern society we have been gradually interested in the elderly and easily come into contact with many medical favors to enhance the quality of life, and the elderly are gradually increasing owing to the development of the basic science such as new diagnostic machinery, operative technique and development of the therapeutics. However, the variation of the environment has increased the possibility to be exposed to the dangerous factors, and it has given rise to the problems about the quality of the elderly life. It has also greatly enlarged in medical as well as social. Therefore, the frequency of the elderly reconstructive surgery to increase the quality of the elderly life by operation without life of death has increased in plastic surgery. So authors thought that the safe operative study is necessary. We reviewed the case of 102 elderly patients (65 years of age), who were operated during the period from 1988 to 1995, and analyzed the age & sex distribution, classification & distribution of diseases in elderly, operation of plastic & reconstructive surgery in elderly, anesthetic methods, underlying diseases, and postoperative complications through the patient records. We also examined postoperative function, aesthetic improvement, progression of the quality of lift by telephone and analyzed the elderly classification and elderly palstic & reconstructive surgery. The results are as follows : 1. There were begin neoplasm, facial bone fracture, aesthetic surgery, skin and soft tissue defect, complicated diabetic mellitus, malignant ulcer, malignant neoplasm. pressure sore in elderly diseases of plastic & reconstructive surgery. 2. We mainly performed skin graft, flap surgery, ORIF, aesthetic surgery. 3. The recovery rate after postoperative evaluation shows that; 1) 52% of total interviewee feel that their QOL has improved. 2) 24% of total interviewee feel that their QOL has not changed. 3) 24% of total interviewee feel that QOL has deteriorated. We report the point at issue and statistical examination with record investigattion.

악안면 손상 환자의 창상감염에 대한 고찰

양현진,하지운,서인석 大韓成形外科學會 1994 Archives of Plastic Surgery Vol.21 No.2

This study comprised 203 patients with maxillofacial injury treated in the Department of Plastic and Reconstructive Surgery at the Kan Nam Sacred Heart Hallym University Hospital between September 1991 and August 1992. With the increasing incidence of high-speed motor vehicle accidents and violent instrument-aided assaults, there has been a concomitent increase in maxillofacial injury. One of the greatest enemies to treat without noticeable cosmetic and functional problems is wound infection. We studies 203 patients, and retrospective study was done for the review and analysis of following issues: sex and age distribution, cause of injury, type of injury, prevallent site of injury, length of laceration, duration from injury to treatment, wound culture and antibiotic sensitivity. The following results were obtained. 1. Of 204 cases, 26 were contaminated: and 38.5% of these contaminated cases were caused by coagulase negative staphylococcus, and in face of healthy persons, 72% of the normal flora is coagulase negative staphylococcus. therefore, most of the causative organism of wound contamination in the facial injury corresponded with the normal flora of face. 2. The influencing factors on the positive culture study were type of maxillo-facial trauma, duration from injury to treatment and presence of gross contamination. 3. In antibiotic sensitivity test, kanamycin, gentamicin and cefazoline were sensitive, minocyclin, cefoperazone and bactrim were relatively sensitive and amphicillin and chloram-phenicol were resistant. 4. Among 191 cases of simple soft tissue injury, authors treated 142 patients of grossly clean mixillofacial soft tissue injury without antibiotics and only 4 patients(2.8%) suffered from minor infection considering these low infection rate, it seems that prevfentive antibiotic therapy is not necessary to treat maxillofacial soft tissue injuries which are simple and grossly clean. 5. we suggest that antibiotics should be given judiciously in selected cases with positive indications: i.e. grossly contaminated injuries, injuries associated with facial bone fracture, and in which there is delay above 3 hours after injury.

각종 성장인자 (GROWTH FACTORS)가 골아 세포주 MC 3T3-E1에 미치는 효과

서인석,이두형 大韓成形外科學會 1991 Archives of Plastic Surgery Vol.18 No.2

Bone formation is a complex process which involves a large number of cellular functions directed toward the synthesis and mineralization of bone matrix. Bone formation is regulated by systemic hormones and by systemic and local growth factors which influence bone replication, differentiation and the actual performance of specialized activities. The effects of hormones on bone metabolism have been studied extensively by a number of investigators and, recently, information about growth factors and their role in bone formation and bone resorption has become available. Our laboratory has been interested in the study of factors produced locally by the skeletal tissue. The aim of this study is to investigate the effects of various growth factors on the growth of osteoblastic clone MC 3T3-El cells. Maximum stimulation of proliferation of osteoblastic cells, relative to corresponding controls, was seen at 0.1%-1% serum and the effects was dose-dependent. With various growth factors, such as IGF-Ⅰ,IGF-Ⅱ,EGF, and FGF, sustained the growth of calvarial cells in culture during multiple passages at 0.1% serum. In DNA synthesis assays, all growth factors stimulate cell proliferation. Stimulation of DNA synthesis peaked at 20ng/㎖ of IGF-Ⅰ, 50ng/㎖ of IGF-Ⅱ, and 20ng/㎖ of EGF, while higher concentrations were inhibitory or stationary. PTH-sensitive adenylate cyclase was stimulated by all growth factors. But the alkaline phosphatase activity decreased slowly in cells grown in all growth factors.