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이탈리안라이그라스 예취시기가 종자 등숙 및 발아에 미치는 영향
徐錫埼,金永斗,朴昊基,朴文洙 韓國作物學會 1997 한국작물학회지 Vol.42 No.1
이탈리안라이그라스의 청초 이용 및 종자 생산가능성을 검토코자 1986년 10월부터 이듬해 6월까지 Tetrone 등 7품종을 공시하여 예취시기를 각각 4월 10일 예취와 4월 30일 예취 및 무예취로하여 시험을 실시한 결과를 요약하면 다음과 같다. 1. 출수기는 K-11, T.N.T 등 품종이 늦었는데 각 품종 모두 예취시기가 늦을수록 출수기가 지연되고, 천입종은 직선적으로 감소하였다. 2. 입형은 입장, 입폭 및 입후 모두 무예취구에 비하여 예취시기가 늦을수록 작아지는 경향이며, 품종간에는 4배체 품종들이 2배체 품종들 보다 컸다. 3. 최초 발아율 및 발아세는 25℃ 에서 15℃ 보다 높았으며, 최종 발아율은 예취시기가 늦어질수록 낮았는데, 온도간에는 15℃ 가 25℃ 보다 다소 높았으며, 품종간에는 출수기가 빨랐던 품종들의 발아율이 높았다. 4. 발아 균일도는 25℃ 가 15℃ 보다 다소 좋았으며, 예취시기가 늦어질수록 낮아졌는데, 품종간에는 K-11 등 출수기가 빨랐던 것들이 발아가 균일 한 경향이며, 평균 발아일수는 25℃ 보다 15℃ 에서 길었고, 예취가 늦을수록 길었다. 5. 등숙관련 형질과 발아율 관계는 예취가 늦어질수록 출수가 늦어 발아율은 직선적으로 낮아지고, 천입종이 무거울수록 발아율이 높아지는 경향이었다. Since seeds of Italian ryegrass should be imported every year, it is necessary to investigate the prossible production of seeds in Italian ryegrass field after rice. Seven Italian ryegrass varieties were planted on October 8, 1986 at National Honam Crop Experiment Station at Iksan and clippings were made on April 10 and April 30 in 1987. Headings of K-11 and T.N.T were earlier than those of Tetrone and Bettina. For all varieties tested, heading date was delayed and 1,000 grain weight was decreased linearly as the clipping was made later. Length, width and thickness of a grain were reduced by clipping and later clipping, while tetraploid varieties produced larger grains than diploid varieties. The germination percentage and germination speed at 25~circC were higher than at 15~circC . The final germination percentage was lower at the later clippings and was higher at 15~circC , and earlier varieties showed higher germination percentage. Uniformity of germination was greater at 25~circC and was decreased as the clipping was made later, and the eariler varieties such as K-11 were germinated more uniformly. Average number of days to germination was longer at 15~circC than at 25~circC and was longer at later clipping. The germination percentage was decreased linearly as the heading was delayed, while it increased linearly as the 1,000 grain weight was increased.
A New Soybean Cultivar, "Sunam" Suitable for Sprout with Early Maturity and Small Seed
서석기,박호기,김경호,오영진,김학신,서덕용,박문수,이문희 한국육종학회 2004 한국육종학회지 Vol.36 No.3
A new soybean variety, Sunam was developed from the cross between Kosuzu and Bukwangkong by soybean breeding laboratory of the National Honam Agricultural Experiment Station (NHAES) in 2002. The preliminary, advanced, and regional yield trials for evaluation and selection were carried out from 1998 to 2002. Sunam had a determinate growth habit, purple flower, grey pubescence, yellow seed coat, brown hilum, and ovoid leaf shape. The maturity date of Sunam was 15 days earlier than the check variety, Pungsan. It has good seed quality for soybean sprout, 100-seed weight being 9.3g and resistance to lodging. It has also been identified to have resistance to soybean mosaic virus. Average yield of Sunam in the regional yield trials for double cropping carried out for three years (2000-2002) was 2.22 ton per hectare.
Kim, Young Jin,Suh, Sug Kee,Oh, Young Jin,Kim, Kyong Ho,Park, Ho Ki,Kim, Seok Dong,Yun, Song Joong 한국콩연구회 2003 韓國콩硏究會誌 Vol.20 No.1
This study was conducted to introduce a gamma-tocopherol methyl transferase gene (γ-TMT) into soybean (Glycien max) to improve tocopherol (vitamin E) composition in soybean seeds. One-day-old cotyledon after germination induced adventitious shoots (98%. 2.6 shoots/cotyledon) directly from cotyledon. The optimal medium for the induction of multiple shoots from cotyledon in 'Pungsmnamulkong'. 'Lx 16'. and 'Ilpumgeomjeongkong'was MS medium supplemented with 2 ㎎/ℓ BAP. but for 'Alchankong. MS medium supplemented with 1 ㎎/ℓ zeatin and 1 ㎎/ℓ IAA 3% sucrose. 4% phytagel and pH 5.8. Somatic embryos were induced from immature cotyledons and cultured on a MS medium containing 40 ㎎/ℓ 2.4-D. The maximum induction of embryos was obtained from cotyledons in a sue of 3-4 ㎜ at pH 7.0. The most efficient system for multiplication and development of somatic embryos is CP6 prcedure with the media MSM6AC/FNL OS3S3GM. Soy I solution (EC 2.9 mS/㎝) was proved to be the most effective solution for the acclimation. Soybean transformation with γ-TMT gene was conducted through somatic embryogenesis. Nineteen regenerated plantlets obtained on the selection medium from 1.460 cotyledons. However, only 9 plantlets were transformed. Putative selected transformants were confirmed for the stable integration of the transgene into the soybean genomic DNA by PCR and Southern blot analyses, and for the expression of the transgene by HPLC analysis. HPLC analysis showed that the content of α-tocopherol in transgenic soybean seeds (AT-I) was approximately 4-fold higher than that of non-transgenic plants. The result indicated the expression of y-TMT transgene and conversion of a part of the γ-tocopherol pool to α-tocopherol by the expressed γ-TMT.Although transformation frequency from the initial explant to whole plant was extremely low (0.7%) until now, the system can be used to transfer foreign genes into soybean cells. Gene transformation was also accomplished by in planta procedures using Agrobacterium tumefaciens. The procedure requires no tissue culture processes as it involves only direct infection of Agrobacterium at the cotyledonary nodes and adjacent regions of germinating soybean seeds or at the leaf axil of soybean seedlings. The length of time that the seeds take to germinate prior to Agrobacterium infection greatly affected infection efficiency of meristematic cells. The optimum time for the infection of germinating seeds was 24 hr after germination.